|
|
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result sql = Extracellular calcium-sensing receptor (CaR) expression and its potential role in parathyroid hormone-related peptide (PTHrP) secretion in the H-500 rat Leydig cell model of humoral hypercalcemia of malignancy.
Extracellular calcium-sensing receptor (CaR) expression and its potential role in parathyroid hormone-related peptide (PTHrP) secretion in the H-500 rat Leydig cell model of humoral hypercalcemia of malignancy.char = H;code = 72
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char = p;code = 112
char = e;code = 101
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char = c;code = 99
char = e;code = 101
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char = H;code = 72
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char = H;code = 72
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char = s;code = 115
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result sql = Humoral hypercalcemia of malignancy (HHM) occurs when secretion of parathyroid hormone-related peptide (PTHrP) by cancer cells causes hypercalcemia in the absence of skeletal metastases. High extracellular calcium (Ca(2+)(o)) increases secretion of PTH-like bioactivity by rat H-500 leydig cells, a transplantable model of HHM, an action potentially mediated by the Ca(2+)(o)-sensing receptor (CaR). In this study we investigated whether H-500 cells express the CaR and, if so, whether CaR agonists modulate PTHrP secretion. Northern blot analysis and RT-PCR revealed bona fide CaR transcript(s), and immunocytochemistry and Western analysis with a specific anti-CaR antiserum demonstrated CaR protein expression in H-500 cells. Furthermore, high Ca(2+)(o) and neomycin stimulated PTHrP secretion dose-dependently with maximal 2.7- and 3.3-fold increases at 5 mM Ca(2+)(o) and 300 microM neomycin, respectively. Thus in HHM caused by H-500 cells, the CaR could participate in a vicious cycle whereby PTHrP-induced increases in Ca(2+)(o) further stimulate PTHrP release and exacerbate hypercalcemia.
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result sql = Extracellular calcium-sensing receptor (CaR) expression and its potential role in parathyroid hormone-related peptide (PTHrP) secretion in the H-500 rat Leydig cell model of humoral hypercalcemia of malignancy.
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char = l;code = 108
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char = d;code = 100
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char = P;code = 80
char = ;code = 32
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char = e;code = 101
char = c;code = 99
char = r;code = 114
char = e;code = 101
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = o;code = 111
char = s;code = 115
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char = d;code = 100
char = e;code = 101
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char = t;code = 116
char = h;code = 104
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char = x;code = 120
char = i;code = 105
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = 2;code = 50
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char = 7;code = 55
char = -;code = 45
char = ;code = 32
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char = d;code = 100
char = ;code = 32
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char = 3;code = 51
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char = f;code = 102
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char = r;code = 114
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char = e;code = 101
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = 5;code = 53
char = ;code = 32
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char = ;code = 32
char = C;code = 67
char = a;code = 97
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char = +;code = 43
char = );code = 41
char = (;code = 40
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char = );code = 41
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = 3;code = 51
char = 0;code = 48
char = 0;code = 48
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char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = M;code = 77
char = ;code = 32
char = n;code = 110
char = e;code = 101
char = o;code = 111
char = m;code = 109
char = y;code = 121
char = c;code = 99
char = i;code = 105
char = n;code = 110
char = ,;code = 44
char = ;code = 32
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char = s;code = 115
char = p;code = 112
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char = t;code = 116
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char = v;code = 118
char = e;code = 101
char = l;code = 108
char = y;code = 121
char = .;code = 46
char = ;code = 32
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char = h;code = 104
char = u;code = 117
char = s;code = 115
char = ;code = 32
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char = n;code = 110
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char = H;code = 72
char = M;code = 77
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char = s;code = 115
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char = 0;code = 48
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char = l;code = 108
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char = o;code = 111
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char = o;code = 111
char = );code = 41
char = ;code = 32
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char = u;code = 117
char = r;code = 114
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = i;code = 105
char = m;code = 109
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = P;code = 80
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char = P;code = 80
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char = l;code = 108
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char = ;code = 32
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char = c;code = 99
char = e;code = 101
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char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = h;code = 104
char = y;code = 121
char = p;code = 112
char = e;code = 101
char = r;code = 114
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = c;code = 99
char = e;code = 101
char = m;code = 109
char = i;code = 105
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result sql = Humoral hypercalcemia of malignancy (HHM) occurs when secretion of parathyroid hormone-related peptide (PTHrP) by cancer cells causes hypercalcemia in the absence of skeletal metastases. High extracellular calcium (Ca(2+)(o)) increases secretion of PTH-like bioactivity by rat H-500 leydig cells, a transplantable model of HHM, an action potentially mediated by the Ca(2+)(o)-sensing receptor (CaR). In this study we investigated whether H-500 cells express the CaR and, if so, whether CaR agonists modulate PTHrP secretion. Northern blot analysis and RT-PCR revealed bona fide CaR transcript(s), and immunocytochemistry and Western analysis with a specific anti-CaR antiserum demonstrated CaR protein expression in H-500 cells. Furthermore, high Ca(2+)(o) and neomycin stimulated PTHrP secretion dose-dependently with maximal 2.7- and 3.3-fold increases at 5 mM Ca(2+)(o) and 300 microM neomycin, respectively. Thus in HHM caused by H-500 cells, the CaR could participate in a vicious cycle whereby PTHrP-induced increases in Ca(2+)(o) further stimulate PTHrP release and exacerbate hypercalcemia.
Publication: Biochem. Biophys. Res. Commun. 2000 Mar 16 ;269(2)::427-32.
Publication Date: 2000 Mar 16
Study Author(s): Sanders, J L;Chattopadhyay, N;Kifor, O;Yamaguchi, T;Brown, E M;
Institution: Endocrine-Hypertension Division, Harvard Medical School, Boston, Massachusetts, 02115, USA. [email protected]
Shortcut link to this study:
Publication Date: 2000 Mar 16
Study Author(s): Sanders, J L;Chattopadhyay, N;Kifor, O;Yamaguchi, T;Brown, E M;
Institution: Endocrine-Hypertension Division, Harvard Medical School, Boston, Massachusetts, 02115, USA. [email protected]
Shortcut link to this study:
Humoral hypercalcemia of malignancy (HHM) occurs when secretion of parathyroid hormone-related peptide (PTHrP) by cancer cells causes hypercalcemia in the absence of skeletal metastases. High extracellular calcium (Ca(2+)(o)) increases secretion of PTH-like bioactivity by rat H-500 leydig cells, a transplantable model of HHM, an action potentially mediated by the Ca(2+)(o)-sensing receptor (CaR). In this study we investigated whether H-500 cells express the CaR and, if so, whether CaR agonists modulate PTHrP secretion. Northern blot analysis and RT-PCR revealed bona fide CaR transcript(s), and immunocytochemistry and Western analysis with a specific anti-CaR antiserum demonstrated CaR protein expression in H-500 cells. Furthermore, high Ca(2+)(o) and neomycin stimulated PTHrP secretion dose-dependently with maximal 2.7- and 3.3-fold increases at 5 mM Ca(2+)(o) and 300 microM neomycin, respectively. Thus in HHM caused by H-500 cells, the CaR could participate in a vicious cycle whereby PTHrP-induced increases in Ca(2+)(o) further stimulate PTHrP release and exacerbate hypercalcemia.
PMID: 10708570
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The order of these related concepts is determined by a statistical analysis of the scientific literature. Highest-frequency concepts appear first.
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