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result sql = Changes in follicular fluid steroids, insulin-like growth factors (IGF) and IGF-binding protein concentration, and proteolytic activity during equine follicular development.
Changes in follicular fluid steroids, insulin-like growth factors (IGF) and IGF-binding protein concentration, and proteolytic activity during equine follicular development.
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char = a;code = 97
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char = g;code = 103
char = e;code = 101
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char = t;code = 116
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char = a;code = 97
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char = i;code = 105
char = n;code = 110
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char = s;code = 115
char = m;code = 109
char = a;code = 97
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char = l;code = 108
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char = r;code = 114
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char = m;code = 109
char = e;code = 101
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = m;code = 109
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char = f;code = 102
char = o;code = 111
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char = l;code = 108
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = E;code = 69
char = s;code = 115
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = o;code = 111
char = l;code = 108
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = r;code = 114
char = o;code = 111
char = s;code = 115
char = t;code = 116
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char = e;code = 101
char = d;code = 100
char = i;code = 105
char = o;code = 111
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char = e;code = 101
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char = c;code = 99
char = o;code = 111
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = n;code = 110
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char = r;code = 114
char = a;code = 97
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char = i;code = 105
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char = n;code = 110
char = s;code = 115
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char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = n;code = 110
char = e;code = 101
char = g;code = 103
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = y;code = 121
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char = c;code = 99
char = o;code = 111
char = r;code = 114
char = r;code = 114
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char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = P;code = 80
char = ;code = 32
char = <;code = 60
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char = .;code = 46
char = 0;code = 48
char = 1;code = 49
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char = ;code = 32
char = w;code = 119
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char = t;code = 116
char = h;code = 104
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
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char = 2;code = 50
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char = -;code = 45
char = 4;code = 52
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = -;code = 45
char = 5;code = 53
char = ;code = 32
char = b;code = 98
char = u;code = 117
char = t;code = 116
char = ;code = 32
char = n;code = 110
char = o;code = 111
char = t;code = 116
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 3;code = 51
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = o;code = 111
char = ;code = 32
char = e;code = 101
char = v;code = 118
char = a;code = 97
char = l;code = 108
char = u;code = 117
char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = o;code = 111
char = l;code = 108
char = y;code = 121
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = ,;code = 44
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = f;code = 102
char = l;code = 108
char = u;code = 117
char = i;code = 105
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = c;code = 99
char = u;code = 117
char = b;code = 98
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = h;code = 104
char = u;code = 117
char = m;code = 109
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = -;code = 45
char = l;code = 108
char = a;code = 97
char = b;code = 98
char = e;code = 101
char = l;code = 108
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 2;code = 50
char = ,;code = 44
char = ;code = 32
char = -;code = 45
char = 3;code = 51
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = -;code = 45
char = 5;code = 53
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = s;code = 115
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char = p;code = 112
char = a;code = 97
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = %;code = 37
char = ;code = 32
char = S;code = 83
char = D;code = 68
char = S;code = 83
char = -;code = 45
char = P;code = 80
char = A;code = 65
char = G;code = 71
char = E;code = 69
char = .;code = 46
char = ;code = 32
char = F;code = 70
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = f;code = 102
char = l;code = 108
char = u;code = 117
char = i;code = 105
char = d;code = 100
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = u;code = 117
char = s;code = 115
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = t;code = 116
char = l;code = 108
char = e;code = 101
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char = o;code = 111
char = r;code = 114
char = ;code = 32
char = n;code = 110
char = o;code = 111
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = o;code = 111
char = l;code = 108
char = y;code = 121
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = -;code = 45
char = l;code = 108
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = e;code = 101
char = l;code = 108
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 2;code = 50
char = ;code = 32
char = o;code = 111
char = r;code = 114
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char = -;code = 45
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char = ,;code = 44
char = ;code = 32
char = a;code = 97
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char = d;code = 100
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char = h;code = 104
char = e;code = 101
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char = s;code = 115
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = a;code = 97
char = m;code = 109
char = o;code = 111
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char = n;code = 110
char = t;code = 116
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = o;code = 111
char = l;code = 108
char = y;code = 121
char = s;code = 115
char = i;code = 105
char = s;code = 115
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 2;code = 50
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
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char = 3;code = 51
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char = d;code = 100
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char = d;code = 100
char = ;code = 32
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char = o;code = 111
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char = d;code = 100
char = i;code = 105
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = ;code = 32
char = >;code = 62
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 1;code = 49
char = 0;code = 48
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char = ;code = 32
char = a;code = 97
char = m;code = 109
char = o;code = 111
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char = g;code = 103
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char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = H;code = 72
char = o;code = 111
char = w;code = 119
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = ,;code = 44
char = ;code = 32
char = m;code = 109
char = o;code = 111
char = r;code = 114
char = e;code = 101
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char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = -;code = 45
char = l;code = 108
char = a;code = 97
char = b;code = 98
char = e;code = 101
char = l;code = 108
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 5;code = 53
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = c;code = 99
char = l;code = 108
char = e;code = 101
char = a;code = 97
char = v;code = 118
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = ;code = 32
char = <;code = 60
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 5;code = 53
char = );code = 41
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
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char = f;code = 102
char = l;code = 108
char = u;code = 117
char = i;code = 105
char = d;code = 100
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = ;code = 32
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = g;code = 103
char = e;code = 101
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char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
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char = c;code = 99
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = e;code = 101
char = c;code = 99
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = d;code = 100
char = u;code = 117
char = r;code = 114
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
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char = p;code = 112
char = h;code = 104
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = a;code = 97
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char = l;code = 108
char = a;code = 97
char = r;code = 114
char = g;code = 103
char = e;code = 101
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char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
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char = u;code = 117
char = r;code = 114
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char = n;code = 110
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char = t;code = 116
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char = e;code = 101
char = a;code = 97
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char = s;code = 115
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = l;code = 108
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char = o;code = 111
char = r;code = 114
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char = m;code = 109
char = e;code = 101
char = d;code = 100
char = i;code = 105
char = u;code = 117
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char = f;code = 102
char = o;code = 111
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char = l;code = 108
char = i;code = 105
char = c;code = 99
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char = e;code = 101
char = s;code = 115
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char = f;code = 102
char = r;code = 114
char = o;code = 111
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char = l;code = 108
char = i;code = 105
char = c;code = 99
char = u;code = 117
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char = a;code = 97
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = l;code = 108
char = u;code = 117
char = t;code = 116
char = e;code = 101
char = a;code = 97
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char = p;code = 112
char = h;code = 104
char = a;code = 97
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char = e;code = 101
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char = m;code = 109
char = a;code = 97
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char = e;code = 101
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char = i;code = 105
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char = h;code = 104
char = a;code = 97
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char = a;code = 97
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char = p;code = 112
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char = t;code = 116
char = e;code = 101
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 5;code = 53
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char = e;code = 101
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char = i;code = 105
char = s;code = 115
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char = e;code = 101
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char = t;code = 116
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char = e;code = 101
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char = i;code = 105
char = n;code = 110
char = a;code = 97
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char = t;code = 116
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char = e;code = 101
char = q;code = 113
char = u;code = 117
char = i;code = 105
char = n;code = 110
char = e;code = 101
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char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = I;code = 73
char = G;code = 71
char = F;code = 70
char = B;code = 66
char = P;code = 80
char = -;code = 45
char = 5;code = 53
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = i;code = 105
char = n;code = 110
char = h;code = 104
char = i;code = 105
char = b;code = 98
char = i;code = 105
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
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char = k;code = 107
char = a;code = 97
char = l;code = 108
char = l;code = 108
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char = r;code = 114
char = e;code = 101
char = i;code = 105
char = n;code = 110
char = /;code = 47
char = s;code = 115
char = e;code = 101
char = r;code = 114
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char = n;code = 110
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = a;code = 97
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result sql = The objective of the present study was to evaluate changes in equine follicular fluid insulin-like growth factor binding protein (IGFBP) proteolytic activity as well as steroid, IGF, and IGFBP concentrations during follicular development in the mare. Mares (n = 14) were classified as either in the follicular phase (n = 8) or luteal phase (n = 6). Follicles (n = 92) were categorized as small (6 to 15 mm; n = 54), medium (16 to 25 mm; n = 23), or large (> 25 mm; n = 15), and follicular fluid was collected. Estradiol and androstenedione levels in follicular fluid were greater (P < 0.05), and IGFBP-3 concentrations tended to be greater (P < 0.10) in large than in small or medium follicles, whereas IGFBP-2, -4, and -5 levels were less (P < 0.05) in large than in small or medium follicles. Estradiol and androstenedione concentrations were negatively correlated (P < 0.01) with IGFBP-2, -4, and -5 but not IGFBP-3 concentrations. To evaluate proteolysis of IGFBP, follicular fluid was incubated with human 125I-labeled IGFBP-2, -3, and -5 and protein separated by 12% SDS-PAGE. Follicular fluid caused little or no proteolysis of 125I-lableled IGFBP-2 or -3, and the small amount of proteolysis of IGFBP-2 and -3 did not differ (P > 0.10) among follicle classes. However, more 125I-labeled IGFBP-5 was cleaved (P < 0.05) by follicular fluid from large follicles collected during the follicular phase than large follicles during the luteal phase, and small or medium follicles from follicular and luteal phase mares indicating that a protease to IGFBP-5 exists in estrogen-dominant equine follicles. This IGFBP-5 protease was inhibited by kallikrein/serine protease and metalloprotease inhibitors. We conclude that the tendency of estrogen-dominant follicles of mares to have greater levels of IGFBP-3 and lesser levels of IGFBP-2 does not appear to be due to differences in proteolysis, whereas changes in IGFBP-5 levels are likely due to changes in activity of a serine protease or metalloprotease. Changes in IGFBP may alter levels of bioavailable IGF that stimulate steroidogenesis and mitogenesis in developing mare follicles.

Publication: J. Anim. Sci. 2002 Jan ;80(1)::179-90.
Publication Date: 2002 Jan
Study Author(s): Bridges, T S;Davidson, T R;Chamberlain, C S;Geisert, R D;Spicer, L J;
Institution: Department of Animal Science, Oklahoma State University, Stillwater 74078, USA.
Shortcut link to this study:
The objective of the present study was to evaluate changes in equine follicular fluid insulin-like growth factor binding protein (IGFBP) proteolytic activity as well as steroid, IGF, and IGFBP concentrations during follicular development in the mare. Mares (n = 14) were classified as either in the follicular phase (n = 8) or luteal phase (n = 6). Follicles (n = 92) were categorized as small (6 to 15 mm; n = 54), medium (16 to 25 mm; n = 23), or large (> 25 mm; n = 15), and follicular fluid was collected. Estradiol and androstenedione levels in follicular fluid were greater (P < 0.05), and IGFBP-3 concentrations tended to be greater (P < 0.10) in large than in small or medium follicles, whereas IGFBP-2, -4, and -5 levels were less (P < 0.05) in large than in small or medium follicles. Estradiol and androstenedione concentrations were negatively correlated (P < 0.01) with IGFBP-2, -4, and -5 but not IGFBP-3 concentrations. To evaluate proteolysis of IGFBP, follicular fluid was incubated with human 125I-labeled IGFBP-2, -3, and -5 and protein separated by 12% SDS-PAGE. Follicular fluid caused little or no proteolysis of 125I-lableled IGFBP-2 or -3, and the small amount of proteolysis of IGFBP-2 and -3 did not differ (P > 0.10) among follicle classes. However, more 125I-labeled IGFBP-5 was cleaved (P < 0.05) by follicular fluid from large follicles collected during the follicular phase than large follicles during the luteal phase, and small or medium follicles from follicular and luteal phase mares indicating that a protease to IGFBP-5 exists in estrogen-dominant equine follicles. This IGFBP-5 protease was inhibited by kallikrein/serine protease and metalloprotease inhibitors. We conclude that the tendency of estrogen-dominant follicles of mares to have greater levels of IGFBP-3 and lesser levels of IGFBP-2 does not appear to be due to differences in proteolysis, whereas changes in IGFBP-5 levels are likely due to changes in activity of a serine protease or metalloprotease. Changes in IGFBP may alter levels of bioavailable IGF that stimulate steroidogenesis and mitogenesis in developing mare follicles.
PMID: 11831514

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