char = C;code = 67 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = -;code = 45 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = 4;code = 52 char = 2;code = 50 char = J;code = 74 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = .;code = 46 result sql = Calcium-dependent apoptotic gene expression in cerulein-treated AR42J cells.
Calcium-dependent apoptotic gene expression in cerulein-treated AR42J cells.char = E;code = 69 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = i;code = 105 char = s;code = 115 char = k;code = 107 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = u;code = 117 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = u;code = 117 char = l;code = 108 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = ,;code = 44 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = i;code = 105 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = b;code = 98 char = a;code = 97 char = x;code = 120 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = p;code = 112 char = 5;code = 53 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = 4;code = 52 char = 2;code = 50 char = J;code = 74 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = B;code = 66 char = A;code = 65 char = P;code = 80 char = T;code = 84 char = A;code = 65 char = -;code = 45 char = A;code = 65 char = M;code = 77 char = .;code = 46 char = ;code = 32 char = R;code = 82 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 7;code = 55 char = );code = 41 char = ;code = 32 char = M;code = 77 char = );code = 41 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = o;code = 111 char = k;code = 107 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = a;code = 97 char = k;code = 107 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;;code = 59 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = r;code = 114 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = B;code = 66 char = A;code = 65 char = P;code = 80 char = T;code = 84 char = A;code = 65 char = -;code = 45 char = A;code = 65 char = M;code = 77 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = D;code = 68 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = 4;code = 52 char = 2;code = 50 char = J;code = 74 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = x;code = 120 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = 5;code = 53 char = 3;code = 51 char = ;code = 32 char = m;code = 109 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = A;code = 65 char = P;code = 80 char = T;code = 84 char = A;code = 65 char = -;code = 45 char = A;code = 65 char = M;code = 77 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = +;code = 43 char = );code = 41 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = m;code = 109 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = b;code = 98 char = a;code = 97 char = x;code = 120 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = p;code = 112 char = 5;code = 53 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Elevated Ca(2+) concentrations within the pancreatic acinar cells represent a risk factor for the development of acute pancreatitis. Apoptosis is an important characteristic of pancreatitis, with induction of apoptotic genes and intraceullar increase of calcium, endonucleases, and protease. The present study, which aims to investigate whether (1) cerulein induces apoptotic gene expression (bax, bid, p53) in pancreatic acinar AR42J cells and (2) cerulein-induced gene expression is mediated by intracellular Ca(2+), monitored the gene expression profile in the cells treated with the Ca(2+) chelator BAPTA-AM. Results showed that cerulein (10(-7) M) evoked an initial peak Ca(2+) signal; a further Ca(2+) signal was induced with second treatment of cerulein. Cerulein-induced Ca(2+) signal could not be detected in the cells treated with the Ca(2+) chelator BAPTA-AM. Cerulein dose-dependently induced apoptosis, determined by DNA fragmentation and pro-apoptotic bid expression in AR42J cells. Cerulein induced bid, bax, and p53 mRNA expression, which was inhibited in the cells treated with cerulein and cultured in the presence of BAPTA-AM. The present results suggest that increase in the free cytosolic Ca(2+) may be the upstream event of apoptotic gene (bax, bid, p53) expression, which contribute to cerulein-induced apoptosis in pancreatic acinar cells.
Publication: Ann. N. Y. Acad. Sci. 2003 Dec ;1010:66-9.
Publication Date: 2003 Dec
Study Author(s): Yu, Ji Hoon;Kim, Hyeyoung;Kim, Kyung Hwan;
Institution: Department of Pharmacology and Institute of Gastroenterology, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Korea.
Shortcut link to this study:
Elevated Ca(2+) concentrations within the pancreatic acinar cells represent a risk factor for the development of acute pancreatitis. Apoptosis is an important characteristic of pancreatitis, with induction of apoptotic genes and intraceullar increase of calcium, endonucleases, and protease. The present study, which aims to investigate whether (1) cerulein induces apoptotic gene expression (bax, bid, p53) in pancreatic acinar AR42J cells and (2) cerulein-induced gene expression is mediated by intracellular Ca(2+), monitored the gene expression profile in the cells treated with the Ca(2+) chelator BAPTA-AM. Results showed that cerulein (10(-7) M) evoked an initial peak Ca(2+) signal; a further Ca(2+) signal was induced with second treatment of cerulein. Cerulein-induced Ca(2+) signal could not be detected in the cells treated with the Ca(2+) chelator BAPTA-AM. Cerulein dose-dependently induced apoptosis, determined by DNA fragmentation and pro-apoptotic bid expression in AR42J cells. Cerulein induced bid, bax, and p53 mRNA expression, which was inhibited in the cells treated with cerulein and cultured in the presence of BAPTA-AM. The present results suggest that increase in the free cytosolic Ca(2+) may be the upstream event of apoptotic gene (bax, bid, p53) expression, which contribute to cerulein-induced apoptosis in pancreatic acinar cells.
PMID: 15033695