|
|
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char = l;code = 108
char = ;code = 32
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char = n;code = 110
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char = a;code = 97
char = n;code = 110
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char = .;code = 46
result sql = Analysis of the vitamin D system in cervical carcinomas, breast cancer and ovarian cancer.
Analysis of the vitamin D system in cervical carcinomas, breast cancer and ovarian cancer.char = 1;code = 49
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char = l;code = 108
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char = p;code = 112
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char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = e;code = 101
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char = e;code = 101
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char = t;code = 116
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char = .;code = 46
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char = R;code = 82
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result sql = 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) is the biologically active metabolite of vitamin D and has been shown to regulate the growth of various cell types. There are two principal enzymes involved in the formation of circulating 1,25(OH)2D3 from vitamin D, the vitamin D 25-hydroxylase (25-OHase) and the 1alpha-hydroxylase (1alpha-OHase). Recently, extrarenal activity of 1alpha-OHase has been reported in various cell types. The aim of this study was to analyze expression of VDR and the main enzymes involved in the synthesis and metabolism of calcitriol in gynecological malignancies and corresponding normal tissue. Expression of VDR, 25-OHase, 1alpha-OHase, and 24-OHase was analyzed in breast carcinomas (BC), ovarian cancer (OC), cervix carcinomas (CC) and normal corresponding tissues using real-time PCR and specific hybridization probes as well as using immunohistochemistry. RNA for VDR, 1alpha-OHase, 24-OHase and 25-OHase was up-regulated in breast cervical and ovarian carcinomas as compared to normal tissue. VDR immunoreactivity was increased in breast and ovarian cancer and in cervix carcinomas as compared to normal corresponding tissue. Our findings indicate that cervical carcinomas, breast cancer and ovarian cancer may be considered as potential targets for prevention or therapy with new vitamin D analogs that exert little or no calcemic side effects or by pharmacological modulation of 1,25(OH)2D3 synthesis and metabolism in these tumor cells.
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result sql = Analysis of the vitamin D system in cervical carcinomas, breast cancer and ovarian cancer.
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char = e;code = 101
char = s;code = 115
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char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = a;code = 97
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = w;code = 119
char = o;code = 111
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char = p;code = 112
char = r;code = 114
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char = n;code = 110
char = c;code = 99
char = i;code = 105
char = p;code = 112
char = a;code = 97
char = l;code = 108
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char = z;code = 122
char = y;code = 121
char = m;code = 109
char = e;code = 101
char = s;code = 115
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char = i;code = 105
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char = i;code = 105
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char = e;code = 101
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char = f;code = 102
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char = m;code = 109
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = c;code = 99
char = i;code = 105
char = r;code = 114
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
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char = n;code = 110
char = g;code = 103
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char = 2;code = 50
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char = O;code = 79
char = H;code = 72
char = );code = 41
char = 2;code = 50
char = D;code = 68
char = 3;code = 51
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = o;code = 111
char = m;code = 109
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char = v;code = 118
char = i;code = 105
char = t;code = 116
char = a;code = 97
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char = i;code = 105
char = n;code = 110
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char = D;code = 68
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = v;code = 118
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char = t;code = 116
char = a;code = 97
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char = i;code = 105
char = n;code = 110
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char = ;code = 32
char = 2;code = 50
char = 5;code = 53
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char = r;code = 114
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char = x;code = 120
char = y;code = 121
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = 2;code = 50
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char = O;code = 79
char = H;code = 72
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = 1;code = 49
char = a;code = 97
char = l;code = 108
char = p;code = 112
char = h;code = 104
char = a;code = 97
char = -;code = 45
char = h;code = 104
char = y;code = 121
char = d;code = 100
char = r;code = 114
char = o;code = 111
char = x;code = 120
char = y;code = 121
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = (;code = 40
char = 1;code = 49
char = a;code = 97
char = l;code = 108
char = p;code = 112
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char = a;code = 97
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char = O;code = 79
char = H;code = 72
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = );code = 41
char = .;code = 46
char = ;code = 32
char = R;code = 82
char = e;code = 101
char = c;code = 99
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char = t;code = 116
char = l;code = 108
char = y;code = 121
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char = ;code = 32
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = r;code = 114
char = e;code = 101
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char = a;code = 97
char = l;code = 108
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
char = y;code = 121
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 1;code = 49
char = a;code = 97
char = l;code = 108
char = p;code = 112
char = h;code = 104
char = a;code = 97
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char = O;code = 79
char = H;code = 72
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = h;code = 104
char = a;code = 97
char = s;code = 115
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char = b;code = 98
char = e;code = 101
char = e;code = 101
char = n;code = 110
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char = r;code = 114
char = e;code = 101
char = p;code = 112
char = o;code = 111
char = r;code = 114
char = t;code = 116
char = e;code = 101
char = d;code = 100
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char = i;code = 105
char = n;code = 110
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char = v;code = 118
char = a;code = 97
char = r;code = 114
char = i;code = 105
char = o;code = 111
char = u;code = 117
char = s;code = 115
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char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = t;code = 116
char = y;code = 121
char = p;code = 112
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = i;code = 105
char = m;code = 109
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = i;code = 105
char = s;code = 115
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char = s;code = 115
char = t;code = 116
char = u;code = 117
char = d;code = 100
char = y;code = 121
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = y;code = 121
char = z;code = 122
char = e;code = 101
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char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = V;code = 86
char = D;code = 68
char = R;code = 82
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = e;code = 101
char = n;code = 110
char = z;code = 122
char = y;code = 121
char = m;code = 109
char = e;code = 101
char = s;code = 115
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char = i;code = 105
char = n;code = 110
char = v;code = 118
char = o;code = 111
char = l;code = 108
char = v;code = 118
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = y;code = 121
char = n;code = 110
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = s;code = 115
char = i;code = 105
char = s;code = 115
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char = a;code = 97
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char = d;code = 100
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char = m;code = 109
char = e;code = 101
char = t;code = 116
char = a;code = 97
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char = o;code = 111
char = l;code = 108
char = i;code = 105
char = s;code = 115
char = m;code = 109
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char = o;code = 111
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char = ;code = 32
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = c;code = 99
char = i;code = 105
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = o;code = 111
char = l;code = 108
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char = i;code = 105
char = n;code = 110
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char = g;code = 103
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char = i;code = 105
char = c;code = 99
char = a;code = 97
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char = m;code = 109
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char = i;code = 105
char = g;code = 103
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char = a;code = 97
char = n;code = 110
char = c;code = 99
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char = e;code = 101
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char = d;code = 100
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char = s;code = 115
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char = g;code = 103
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char = a;code = 97
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char = t;code = 116
char = i;code = 105
char = s;code = 115
char = s;code = 115
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char = .;code = 46
char = ;code = 32
char = E;code = 69
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char = p;code = 112
char = r;code = 114
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char = s;code = 115
char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = o;code = 111
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char = ;code = 32
char = V;code = 86
char = D;code = 68
char = R;code = 82
char = ,;code = 44
char = ;code = 32
char = 2;code = 50
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char = -;code = 45
char = O;code = 79
char = H;code = 72
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ,;code = 44
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char = 1;code = 49
char = a;code = 97
char = l;code = 108
char = p;code = 112
char = h;code = 104
char = a;code = 97
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char = O;code = 79
char = H;code = 72
char = a;code = 97
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char = ,;code = 44
char = ;code = 32
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char = -;code = 45
char = O;code = 79
char = H;code = 72
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = w;code = 119
char = a;code = 97
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char = ;code = 32
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char = a;code = 97
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char = y;code = 121
char = z;code = 122
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char = d;code = 100
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char = i;code = 105
char = n;code = 110
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char = b;code = 98
char = r;code = 114
char = e;code = 101
char = a;code = 97
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char = t;code = 116
char = ;code = 32
char = c;code = 99
char = a;code = 97
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char = c;code = 99
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char = n;code = 110
char = o;code = 111
char = m;code = 109
char = a;code = 97
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char = ;code = 32
char = (;code = 40
char = B;code = 66
char = C;code = 67
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = o;code = 111
char = v;code = 118
char = a;code = 97
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char = i;code = 105
char = a;code = 97
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char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
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char = O;code = 79
char = C;code = 67
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = r;code = 114
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char = i;code = 105
char = x;code = 120
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char = c;code = 99
char = a;code = 97
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char = c;code = 99
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char = n;code = 110
char = o;code = 111
char = m;code = 109
char = a;code = 97
char = s;code = 115
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char = (;code = 40
char = C;code = 67
char = C;code = 67
char = );code = 41
char = ;code = 32
char = a;code = 97
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char = d;code = 100
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char = n;code = 110
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char = m;code = 109
char = a;code = 97
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char = ;code = 32
char = c;code = 99
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char = r;code = 114
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char = e;code = 101
char = s;code = 115
char = p;code = 112
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char = d;code = 100
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char = n;code = 110
char = g;code = 103
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char = s;code = 115
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char = e;code = 101
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char = u;code = 117
char = s;code = 115
char = i;code = 105
char = n;code = 110
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char = r;code = 114
char = e;code = 101
char = a;code = 97
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char = -;code = 45
char = t;code = 116
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char = m;code = 109
char = e;code = 101
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char = P;code = 80
char = C;code = 67
char = R;code = 82
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = s;code = 115
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char = e;code = 101
char = c;code = 99
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char = f;code = 102
char = i;code = 105
char = c;code = 99
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char = s;code = 115
char = t;code = 116
char = r;code = 114
char = y;code = 121
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char = ;code = 32
char = R;code = 82
char = N;code = 78
char = A;code = 65
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
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char = V;code = 86
char = D;code = 68
char = R;code = 82
char = ,;code = 44
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char = 1;code = 49
char = a;code = 97
char = l;code = 108
char = p;code = 112
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char = a;code = 97
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char = O;code = 79
char = H;code = 72
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = ,;code = 44
char = ;code = 32
char = 2;code = 50
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char = O;code = 79
char = H;code = 72
char = a;code = 97
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char = e;code = 101
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char = O;code = 79
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char = a;code = 97
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char = e;code = 101
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char = a;code = 97
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char = u;code = 117
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char = a;code = 97
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char = a;code = 97
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char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = t;code = 116
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char = a;code = 97
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char = i;code = 105
char = s;code = 115
char = s;code = 115
char = u;code = 117
char = e;code = 101
char = .;code = 46
char = ;code = 32
char = V;code = 86
char = D;code = 68
char = R;code = 82
char = ;code = 32
char = i;code = 105
char = m;code = 109
char = m;code = 109
char = u;code = 117
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char = o;code = 111
char = r;code = 114
char = e;code = 101
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
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char = t;code = 116
char = y;code = 121
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char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = i;code = 105
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char = a;code = 97
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char = b;code = 98
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char = e;code = 101
char = a;code = 97
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char = t;code = 116
char = ;code = 32
char = a;code = 97
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char = d;code = 100
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result sql = 1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) is the biologically active metabolite of vitamin D and has been shown to regulate the growth of various cell types. There are two principal enzymes involved in the formation of circulating 1,25(OH)2D3 from vitamin D, the vitamin D 25-hydroxylase (25-OHase) and the 1alpha-hydroxylase (1alpha-OHase). Recently, extrarenal activity of 1alpha-OHase has been reported in various cell types. The aim of this study was to analyze expression of VDR and the main enzymes involved in the synthesis and metabolism of calcitriol in gynecological malignancies and corresponding normal tissue. Expression of VDR, 25-OHase, 1alpha-OHase, and 24-OHase was analyzed in breast carcinomas (BC), ovarian cancer (OC), cervix carcinomas (CC) and normal corresponding tissues using real-time PCR and specific hybridization probes as well as using immunohistochemistry. RNA for VDR, 1alpha-OHase, 24-OHase and 25-OHase was up-regulated in breast cervical and ovarian carcinomas as compared to normal tissue. VDR immunoreactivity was increased in breast and ovarian cancer and in cervix carcinomas as compared to normal corresponding tissue. Our findings indicate that cervical carcinomas, breast cancer and ovarian cancer may be considered as potential targets for prevention or therapy with new vitamin D analogs that exert little or no calcemic side effects or by pharmacological modulation of 1,25(OH)2D3 synthesis and metabolism in these tumor cells.
Publication: Recent Results Cancer Res. 2003 ;164:239-46.
Publication Date: 2003
Study Author(s): Friedrich, Michael;Rafi, Leyla;Mitschele, Tanja;Tilgen, Wolfgang;Schmidt, Werner;Reichrath, Jörg;
Institution: Department of Dermatology, Universitätskliniken des Saarlandes, Homburg/Saar, Germany.
Shortcut link to this study:
Publication Date: 2003
Study Author(s): Friedrich, Michael;Rafi, Leyla;Mitschele, Tanja;Tilgen, Wolfgang;Schmidt, Werner;Reichrath, Jörg;
Institution: Department of Dermatology, Universitätskliniken des Saarlandes, Homburg/Saar, Germany.
Shortcut link to this study:
1,25-Dihydroxyvitamin D3 (1,25(OH)2D3) is the biologically active metabolite of vitamin D and has been shown to regulate the growth of various cell types. There are two principal enzymes involved in the formation of circulating 1,25(OH)2D3 from vitamin D, the vitamin D 25-hydroxylase (25-OHase) and the 1alpha-hydroxylase (1alpha-OHase). Recently, extrarenal activity of 1alpha-OHase has been reported in various cell types. The aim of this study was to analyze expression of VDR and the main enzymes involved in the synthesis and metabolism of calcitriol in gynecological malignancies and corresponding normal tissue. Expression of VDR, 25-OHase, 1alpha-OHase, and 24-OHase was analyzed in breast carcinomas (BC), ovarian cancer (OC), cervix carcinomas (CC) and normal corresponding tissues using real-time PCR and specific hybridization probes as well as using immunohistochemistry. RNA for VDR, 1alpha-OHase, 24-OHase and 25-OHase was up-regulated in breast cervical and ovarian carcinomas as compared to normal tissue. VDR immunoreactivity was increased in breast and ovarian cancer and in cervix carcinomas as compared to normal corresponding tissue. Our findings indicate that cervical carcinomas, breast cancer and ovarian cancer may be considered as potential targets for prevention or therapy with new vitamin D analogs that exert little or no calcemic side effects or by pharmacological modulation of 1,25(OH)2D3 synthesis and metabolism in these tumor cells.
PMID: 12899526
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