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char = ;code = 32
char = n;code = 110
char = e;code = 101
char = w;code = 119
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char = m;code = 109
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char = ;code = 32
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char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = w;code = 119
char = i;code = 105
char = t;code = 116
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char = t;code = 116
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char = l;code = 108
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char = c;code = 99
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char = g;code = 103
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = l;code = 108
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = v;code = 118
char = i;code = 105
char = a;code = 97
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = t;code = 116
char = a;code = 97
char = g;code = 103
char = o;code = 111
char = n;code = 110
char = i;code = 105
char = z;code = 122
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = C;code = 67
char = a;code = 97
char = 2;code = 50
char = +;code = 43
char = /;code = 47
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = m;code = 109
char = o;code = 111
char = d;code = 100
char = u;code = 117
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = f;code = 102
char = u;code = 117
char = n;code = 110
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = .;code = 46
result sql = A new curcumin derivative, HBC, interferes with the cell cycle progression of colon cancer cells via antagonization of the Ca2+/calmodulin function.
A new curcumin derivative, HBC, interferes with the cell cycle progression of colon cancer cells via antagonization of the Ca2+/calmodulin function.
char = H;code = 72
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char = C;code = 67
char = ;code = 32
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char = [;code = 91
char = 3;code = 51
char = ,;code = 44
char = 5;code = 53
char = -;code = 45
char = B;code = 66
char = i;code = 105
char = s;code = 115
char = -;code = 45
char = [;code = 91
char = 2;code = 50
char = -;code = 45
char = (;code = 40
char = 4;code = 52
char = -;code = 45
char = h;code = 104
char = y;code = 121
char = d;code = 100
char = r;code = 114
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char = x;code = 120
char = y;code = 121
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char = 3;code = 51
char = -;code = 45
char = m;code = 109
char = e;code = 101
char = t;code = 116
char = h;code = 104
char = o;code = 111
char = x;code = 120
char = y;code = 121
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char = p;code = 112
char = h;code = 104
char = e;code = 101
char = n;code = 110
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char = l;code = 108
char = );code = 41
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char = e;code = 101
char = t;code = 116
char = h;code = 104
char = y;code = 121
char = l;code = 108
char = ];code = 93
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char = 4;code = 52
char = ,;code = 44
char = 5;code = 53
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char = d;code = 100
char = i;code = 105
char = h;code = 104
char = y;code = 121
char = d;code = 100
char = r;code = 114
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char = r;code = 114
char = a;code = 97
char = z;code = 122
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char = ];code = 93
char = -;code = 45
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char = z;code = 122
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char = ;code = 32
char = a;code = 97
char = c;code = 99
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char = d;code = 100
char = );code = 41
char = ;code = 32
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = l;code = 108
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char = ;code = 32
char = d;code = 100
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = o;code = 111
char = p;code = 112
char = e;code = 101
char = d;code = 100
char = ;code = 32
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char = r;code = 114
char = c;code = 99
char = u;code = 117
char = m;code = 109
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char = ;code = 32
char = d;code = 100
char = e;code = 101
char = r;code = 114
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char = v;code = 118
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
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char = w;code = 119
char = h;code = 104
char = i;code = 105
char = c;code = 99
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char = ;code = 32
char = e;code = 101
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char = h;code = 104
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char = b;code = 98
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char = t;code = 116
char = s;code = 115
char = ;code = 32
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char = t;code = 116
char = e;code = 101
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char = t;code = 116
char = ;code = 32
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char = i;code = 105
char = b;code = 98
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char = ;code = 32
char = a;code = 97
char = g;code = 103
char = a;code = 97
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char = o;code = 111
char = f;code = 102
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char = e;code = 101
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char = a;code = 97
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char = ;code = 32
char = t;code = 116
char = u;code = 117
char = m;code = 109
char = o;code = 111
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char = c;code = 99
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char = l;code = 108
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char = i;code = 105
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char = .;code = 46
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char = I;code = 73
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char = C;code = 67
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char = 2;code = 50
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char = ;code = 32
char = (;code = 40
char = C;code = 67
char = a;code = 97
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char = /;code = 47
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char = a;code = 97
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char = );code = 41
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char = o;code = 111
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char = ;code = 32
char = H;code = 72
char = B;code = 66
char = C;code = 67
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char = u;code = 117
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char = n;code = 110
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char = ;code = 32
char = C;code = 67
char = a;code = 97
char = 2;code = 50
char = +;code = 43
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char = s;code = 115
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char = c;code = 99
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char = n;code = 110
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char = C;code = 67
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result sql = HBC (4-[3,5-Bis-[2-(4-hydroxy-3-methoxy-phenyl)-ethyl]-4,5-dihydro-pyrazol-1-yl]-benzoic acid) is a recently developed curcumin derivative which exhibits potent inhibitory activities against the proliferation of several tumor cell lines. In the present study, we identified Ca2+/calmodulin (Ca2+/CaM) as a direct target protein of HBC using phage display biopanning. Ca2+/CaM-expressing phages specifically bound to the immobilized HBC, and the binding was Ca2+ dependent. Moreover, flexible docking modeling demonstrated that HBC is compatible with the binding cavity for a known inhibitor, W7, in the C-terminal hydrophobic pocket of Ca2+/CaM. In biological systems, HBC induced prolonged phosphorylation of ERK1/2 and activated p21(WAF1) expression, resulting in the induction of G0/G1 cell cycle arrest in HCT15 colon cancer cells. These results suggest that HBC inhibits the cell cycle progression of colon cancer cells via antagonizing of Ca2+/CaM functions.

Publication: Chem. Biol. 2004 Oct ;11(10)::1455-63.
Publication Date: 2004 Oct
Study Author(s): Shim, Joong Sup;Lee, Jiyong;Park, Hyun-Ju;Park, So-Jung;Kwon, Ho Jeong;
Institution: Chemical Genomics National Research Laboratory, Department of Bioscience and Biotechnology, Institute of Bioscience, Sejong University, Seoul 143-747, Korea.
Shortcut link to this study:
HBC (4-[3,5-Bis-[2-(4-hydroxy-3-methoxy-phenyl)-ethyl]-4,5-dihydro-pyrazol-1-yl]-benzoic acid) is a recently developed curcumin derivative which exhibits potent inhibitory activities against the proliferation of several tumor cell lines. In the present study, we identified Ca2+/calmodulin (Ca2+/CaM) as a direct target protein of HBC using phage display biopanning. Ca2+/CaM-expressing phages specifically bound to the immobilized HBC, and the binding was Ca2+ dependent. Moreover, flexible docking modeling demonstrated that HBC is compatible with the binding cavity for a known inhibitor, W7, in the C-terminal hydrophobic pocket of Ca2+/CaM. In biological systems, HBC induced prolonged phosphorylation of ERK1/2 and activated p21(WAF1) expression, resulting in the induction of G0/G1 cell cycle arrest in HCT15 colon cancer cells. These results suggest that HBC inhibits the cell cycle progression of colon cancer cells via antagonizing of Ca2+/CaM functions.
PMID: 15489172

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