char = S;code = 83 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = -;code = 45 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = l;code = 108 char = o;code = 111 char = b;code = 98 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = C;code = 67 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = -;code = 45 char = r;code = 114 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = .;code = 46 result sql = Studies on unfolded beta-microglobulin at C-terminal in dialysis-related amyloidosis.
Studies on unfolded beta-microglobulin at C-terminal in dialysis-related amyloidosis.char = <;code = 60 char = b;code = 98 char = >;code = 62 char = B;code = 66 char = A;code = 65 char = C;code = 67 char = K;code = 75 char = G;code = 71 char = R;code = 82 char = O;code = 79 char = U;code = 85 char = N;code = 78 char = D;code = 68 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = 1;code = 49 char = 9;code = 57 char = 9;code = 57 char = 7;code = 55 char = ,;code = 44 char = ;code = 32 char = S;code = 83 char = t;code = 116 char = o;code = 111 char = p;code = 112 char = p;code = 112 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = ;code = 32 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = c;code = 99 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = b;code = 98 char = o;code = 111 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = -;code = 45 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = l;code = 108 char = o;code = 111 char = b;code = 98 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = );code = 41 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = p;code = 112 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = b;code = 98 char = r;code = 114 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = e;code = 101 char = a;code = 97 char = n;code = 110 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = l;code = 108 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = i;code = 105 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = y;code = 121 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = s;code = 115 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = b;code = 98 char = r;code = 114 char = i;code = 105 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = r;code = 114 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = >;code = 62 char = M;code = 77 char = E;code = 69 char = T;code = 84 char = H;code = 72 char = O;code = 79 char = D;code = 68 char = S;code = 83 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = c;code = 99 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = b;code = 98 char = o;code = 111 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ;code = 32 char = (;code = 40 char = m;code = 109 char = A;code = 65 char = b;code = 98 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = m;code = 109 char = a;code = 97 char = ;code = 32 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = s;code = 115 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = 1;code = 49 char = 8;code = 56 char = ;code = 32 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = -;code = 45 char = r;code = 114 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = D;code = 68 char = R;code = 82 char = A;code = 65 char = );code = 41 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = >;code = 62 char = R;code = 82 char = E;code = 69 char = S;code = 83 char = U;code = 85 char = L;code = 76 char = T;code = 84 char = S;code = 83 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = A;code = 65 char = b;code = 98 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = i;code = 105 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = A;code = 65 char = b;code = 98 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = .;code = 46 char = ;code = 32 char = S;code = 83 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = l;code = 108 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = p;code = 112 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = s;code = 115 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = A;code = 65 char = b;code = 98 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = m;code = 109 char = u;code = 117 char = n;code = 110 char = o;code = 111 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = t;code = 116 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = m;code = 109 char = m;code = 109 char = u;code = 117 char = n;code = 110 char = o;code = 111 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = A;code = 65 char = b;code = 98 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 7;code = 55 char = ;code = 32 char = C;code = 67 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = D;code = 68 char = R;code = 82 char = A;code = 65 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = A;code = 65 char = b;code = 98 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = ,;code = 44 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = C;code = 67 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = s;code = 115 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = >;code = 62 char = C;code = 67 char = O;code = 79 char = N;code = 78 char = C;code = 67 char = L;code = 76 char = U;code = 85 char = S;code = 83 char = I;code = 73 char = O;code = 79 char = N;code = 78 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = c;code = 99 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = b;code = 98 char = o;code = 111 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = 9;code = 57 char = 2;code = 50 char = -;code = 45 char = 9;code = 57 char = 9;code = 57 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = v;code = 118 char = o;code = 111 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = m;code = 109 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = C;code = 67 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = C;code = 67 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = C;code = 67 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = .;code = 46 result sql = BACKGROUND : In 1997, Stoppini et al reported that monoclonal antibody specific to the C-terminal 92-99 of beta(2)-microglobulin (beta(2)m) had been capable of inhibiting fibrillogenesis of beta(2)m in vitro. Meanwhile, recent studies have indicated that an acidifying procedure can unfold conformation of the precursor protein, leading to fibril formation of beta(2)m as well as a transthyretin.METHODS : We thus prepared monoclonal antibody specific to the C-terminal 92-99 (mAb 92-99), and investigated its reactivity in plasma ultrafiltrate and amyloid tissues from 18 hemodialysis patients with dialysis-related amyloidosis (DRA).RESULTS : beta(2)m extracted from ultrafiltrate showed no reaction for mAb 92-99, whereas acidified beta(2)m from ultrafiltrate showed a reaction for mAb 92-99. Similarly, a homogenate of carpal amyloid tissues showed a strong reaction for mAb 92-99 on immunoblotting. Immunohistochemical study showed also a distinct staining for mAb 92-99 in 7 Congophilic specimens from DRA patients. More interestingly, staining for mAb 92-99 could be found in most, though not all, non-Congophilic tissues.CONCLUSION : This study demonstrates that the monoclonal antibody specific to the C-terminal 92-99 of beta(2)m can detect the conformational intermediate in amyloidogenesis of beta(2)m ex vivo, and demonstrates that an unfolded beta(2)m at C-terminal could be found not only in Congophilic area but even in non-Congophilic area as well.
Publication: Kidney Int. 2005 Jan ;67(1)::314-20.
Publication Date: 2005 Jan
Study Author(s): Motomiya, Yoshihiro;Ando, Yukio;Haraoka, Katsuki;Sun, Xuguo;Morita, Hiroyuki;Amano, Izumi;Uchimura, Tomonori;Maruyama, Ikuro;
Institution: Suiyukai Clinic, Nara, Japan.
[email protected]
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BACKGROUND : In 1997, Stoppini et al reported that monoclonal antibody specific to the C-terminal 92-99 of beta(2)-microglobulin (beta(2)m) had been capable of inhibiting fibrillogenesis of beta(2)m in vitro. Meanwhile, recent studies have indicated that an acidifying procedure can unfold conformation of the precursor protein, leading to fibril formation of beta(2)m as well as a transthyretin.METHODS : We thus prepared monoclonal antibody specific to the C-terminal 92-99 (mAb 92-99), and investigated its reactivity in plasma ultrafiltrate and amyloid tissues from 18 hemodialysis patients with dialysis-related amyloidosis (DRA).RESULTS : beta(2)m extracted from ultrafiltrate showed no reaction for mAb 92-99, whereas acidified beta(2)m from ultrafiltrate showed a reaction for mAb 92-99. Similarly, a homogenate of carpal amyloid tissues showed a strong reaction for mAb 92-99 on immunoblotting. Immunohistochemical study showed also a distinct staining for mAb 92-99 in 7 Congophilic specimens from DRA patients. More interestingly, staining for mAb 92-99 could be found in most, though not all, non-Congophilic tissues.CONCLUSION : This study demonstrates that the monoclonal antibody specific to the C-terminal 92-99 of beta(2)m can detect the conformational intermediate in amyloidogenesis of beta(2)m ex vivo, and demonstrates that an unfolded beta(2)m at C-terminal could be found not only in Congophilic area but even in non-Congophilic area as well.
PMID: 15610257