char = O;code = 79 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = x;code = 120 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = T;code = 84 char = H;code = 72 char = P;code = 80 char = -;code = 45 char = 1;code = 49 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = :;code = 58 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = A;code = 65 char = T;code = 84 char = P;code = 80 char = -;code = 45 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = t;code = 116 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = A;code = 65 char = 1;code = 49 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = .;code = 46 result sql = Oxidative stress influences cholesterol efflux in THP-1 macrophages: role of ATP-binding cassette A1 and nuclear factors.
Oxidative stress influences cholesterol efflux in THP-1 macrophages: role of ATP-binding cassette A1 and nuclear factors.char = <;code = 60 char = b;code = 98 char = >;code = 62 char = O;code = 79 char = B;code = 66 char = J;code = 74 char = E;code = 69 char = C;code = 67 char = T;code = 84 char = I;code = 73 char = V;code = 86 char = E;code = 69 char = S;code = 83 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = U;code = 85 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = o;code = 111 char = l;code = 108 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = a;code = 97 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = a;code = 97 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = O;code = 79 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = i;code = 105 char = m;code = 109 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = y;code = 121 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = P;code = 80 char = P;code = 80 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = L;code = 76 char = X;code = 88 char = R;code = 82 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = i;code = 105 char = r;code = 114 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = >;code = 62 char = M;code = 77 char = E;code = 69 char = T;code = 84 char = H;code = 72 char = O;code = 79 char = D;code = 68 char = S;code = 83 char = ;code = 32 char = A;code = 65 char = N;code = 78 char = D;code = 68 char = ;code = 32 char = R;code = 82 char = E;code = 69 char = S;code = 83 char = U;code = 85 char = L;code = 76 char = T;code = 84 char = S;code = 83 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = u;code = 117 char = b;code = 98 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = T;code = 84 char = H;code = 72 char = P;code = 80 char = -;code = 45 char = 1;code = 49 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = );code = 41 char = /;code = 47 char = a;code = 97 char = s;code = 115 char = c;code = 99 char = o;code = 111 char = r;code = 114 char = b;code = 98 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = );code = 41 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = 4;code = 52 char = ;code = 32 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = h;code = 104 char = y;code = 121 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = 2;code = 50 char = 2;code = 50 char = 0;code = 48 char = %;code = 37 char = ,;code = 44 char = ;code = 32 char = P;code = 80 char = ;code = 32 char = <;code = 60 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 0;code = 48 char = 1;code = 49 char = );code = 41 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = p;code = 112 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = x;code = 120 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = u;code = 117 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = A;code = 65 char = B;code = 66 char = C;code = 67 char = A;code = 65 char = I;code = 73 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = O;code = 79 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = x;code = 120 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = R;code = 82 char = -;code = 45 char = B;code = 66 char = I;code = 73 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = C;code = 67 char = D;code = 68 char = 3;code = 51 char = 6;code = 54 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = p;code = 112 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = E;code = 69 char = x;code = 120 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = R;code = 82 char = T;code = 84 char = -;code = 45 char = P;code = 80 char = C;code = 67 char = R;code = 82 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = A;code = 65 char = B;code = 66 char = C;code = 67 char = A;code = 65 char = I;code = 73 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = L;code = 76 char = X;code = 88 char = R;code = 82 char = a;code = 97 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = L;code = 76 char = X;code = 88 char = R;code = 82 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = P;code = 80 char = P;code = 80 char = A;code = 65 char = R;code = 82 char = a;code = 97 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = P;code = 80 char = P;code = 80 char = A;code = 65 char = R;code = 82 char = g;code = 103 char = a;code = 97 char = m;code = 109 char = m;code = 109 char = a;code = 97 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = T;code = 84 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = x;code = 120 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = H;code = 72 char = T;code = 84 char = );code = 41 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = d;code = 100 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = a;code = 97 char = s;code = 115 char = c;code = 99 char = o;code = 111 char = r;code = 114 char = b;code = 98 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = v;code = 118 char = e;code = 101 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = p;code = 112 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 char = <;code = 60 char = b;code = 98 char = >;code = 62 char = C;code = 67 char = O;code = 79 char = N;code = 78 char = C;code = 67 char = L;code = 76 char = U;code = 85 char = S;code = 83 char = I;code = 73 char = O;code = 79 char = N;code = 78 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = O;code = 79 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = i;code = 105 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = a;code = 97 char = i;code = 105 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = o;code = 111 char = l;code = 108 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = x;code = 120 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = a;code = 97 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = .;code = 46 result sql = OBJECTIVES : Understanding the mechanisms involved in oxidative stress-induced foam cell formation is of fundamental importance for atherosclerosis. Our aim was to characterize the effects of oxidative stress on key receptors of macrophage cholesterol homeostasis, on the nuclear transcription factors PPAR and LXR regulating their expression, and on macrophage cholesterol handling.METHODS AND RESULTS : The incubation of macrophages derived from the human monocyte cell line THP-1 with iron (100 microm)/ascorbate (1000 microm) for a period of 4 h induced a strong peroxidation, as demonstrated by the elevation of malondialdehyde (220%, P < 0.001). The production of lipid peroxidation affected cholesterol efflux, which was probably due to decreased ABCAI gene and protein expression. On the other hand, cholesterol influx remained unchanged as did the mRNA and protein levels of SR-BI and CD36, important protein receptors that participate in cholesterol import. Experiments using RT-PCR showed that the ABCAI modulation was orchestrated by the nuclear receptors LXRalpha, LXRbeta, PPARalpha, and PPARgamma. Treatment with powerful antioxidants (Trolox and BHT) prevented the adverse effects of iron-ascorbate on cholesterol movement, conceivably supporting the role of oxidative stress.CONCLUSION : Our results show that oxidative stress can directly be induced in macrophages and concomitantly impairs the expression of receptors involved in cholesterol flux, which could influence foam cell formation and atherosclerosis development.
Publication: Cardiovasc. Res. 2006 Dec 1 ;72(3)::473-82.
Publication Date: 2006 Dec 1
Study Author(s): Marcil, Valérie;Delvin, Edgard;Sané, Alain Théophile;Tremblay, André;Levy, Emile;
Institution: Centre de Recherche, CHU-Sainte-Justine, Université de Montréal, 3175, Côte Ste-Catherine, Montréal (Québec), Canada H3T 1C5.
Shortcut link to this study:
OBJECTIVES : Understanding the mechanisms involved in oxidative stress-induced foam cell formation is of fundamental importance for atherosclerosis. Our aim was to characterize the effects of oxidative stress on key receptors of macrophage cholesterol homeostasis, on the nuclear transcription factors PPAR and LXR regulating their expression, and on macrophage cholesterol handling.METHODS AND RESULTS : The incubation of macrophages derived from the human monocyte cell line THP-1 with iron (100 microm)/ascorbate (1000 microm) for a period of 4 h induced a strong peroxidation, as demonstrated by the elevation of malondialdehyde (220%, P < 0.001). The production of lipid peroxidation affected cholesterol efflux, which was probably due to decreased ABCAI gene and protein expression. On the other hand, cholesterol influx remained unchanged as did the mRNA and protein levels of SR-BI and CD36, important protein receptors that participate in cholesterol import. Experiments using RT-PCR showed that the ABCAI modulation was orchestrated by the nuclear receptors LXRalpha, LXRbeta, PPARalpha, and PPARgamma. Treatment with powerful antioxidants (Trolox and BHT) prevented the adverse effects of iron-ascorbate on cholesterol movement, conceivably supporting the role of oxidative stress.CONCLUSION : Our results show that oxidative stress can directly be induced in macrophages and concomitantly impairs the expression of receptors involved in cholesterol flux, which could influence foam cell formation and atherosclerosis development.
PMID: 17070507