char = E;code = 69 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 3;code = 51 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = .;code = 46 result sql = Effects of quercetin and quercetin 3-glucuronide on the expression of bone sialoprotein gene.
Effects of quercetin and quercetin 3-glucuronide on the expression of bone sialoprotein gene.char = Q;code = 81 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = a;code = 97 char = v;code = 118 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = -;code = 45 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = a;code = 97 char = v;code = 118 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = v;code = 118 char = e;code = 101 char = g;code = 103 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = i;code = 105 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = i;code = 105 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = B;code = 66 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = );code = 41 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = x;code = 120 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = s;code = 115 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = h;code = 104 char = y;code = 121 char = d;code = 100 char = r;code = 114 char = o;code = 111 char = x;code = 120 char = y;code = 121 char = a;code = 97 char = p;code = 112 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = r;code = 114 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = P;code = 80 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = l;code = 108 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = f;code = 102 char = l;code = 108 char = a;code = 97 char = v;code = 118 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = );code = 41 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = C;code = 67 char = C;code = 67 char = A;code = 65 char = A;code = 65 char = T;code = 84 char = ;code = 32 char = b;code = 98 char = o;code = 111 char = x;code = 120 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = o;code = 111 char = b;code = 98 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = -;code = 45 char = l;code = 108 char = i;code = 105 char = k;code = 107 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = R;code = 82 char = O;code = 79 char = S;code = 83 char = ;code = 32 char = 1;code = 49 char = 7;code = 55 char = /;code = 47 char = 2;code = 50 char = .;code = 46 char = 8;code = 56 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = j;code = 106 char = u;code = 117 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 3;code = 51 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = Q;code = 81 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 3;code = 51 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = 5;code = 53 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = M;code = 77 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = m;code = 109 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = 1;code = 49 char = 2;code = 50 char = ;code = 32 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = b;code = 98 char = f;code = 102 char = a;code = 97 char = 1;code = 49 char = /;code = 47 char = R;code = 82 char = u;code = 117 char = n;code = 110 char = x;code = 120 char = 2;code = 50 char = ;code = 32 char = m;code = 109 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = 1;code = 49 char = 2;code = 50 char = ;code = 32 char = h;code = 104 char = .;code = 46 char = ;code = 32 char = F;code = 70 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = -;code = 45 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = p;code = 112 char = L;code = 76 char = U;code = 85 char = C;code = 67 char = 3;code = 51 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = l;code = 108 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = -;code = 45 char = 1;code = 49 char = 1;code = 49 char = 6;code = 54 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = -;code = 45 char = 4;code = 52 char = 3;code = 51 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = m;code = 109 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = l;code = 108 char = u;code = 117 char = d;code = 100 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = 2;code = 50 char = ;code = 32 char = b;code = 98 char = p;code = 112 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = C;code = 67 char = C;code = 67 char = A;code = 65 char = A;code = 65 char = T;code = 84 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = F;code = 70 char = R;code = 82 char = E;code = 69 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = C;code = 67 char = A;code = 65 char = A;code = 65 char = T;code = 84 char = -;code = 45 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = x;code = 120 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = f;code = 102 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = o;code = 111 char = r;code = 114 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = i;code = 105 char = f;code = 102 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = Q;code = 81 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = F;code = 70 char = R;code = 82 char = E;code = 69 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = 3;code = 51 char = ';code = 39 char = -;code = 45 char = F;code = 70 char = R;code = 82 char = E;code = 69 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 3;code = 51 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = m;code = 109 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = C;code = 67 char = C;code = 67 char = A;code = 65 char = A;code = 65 char = T;code = 84 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = F;code = 70 char = R;code = 82 char = E;code = 69 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = S;code = 83 char = P;code = 80 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Quercetin is a typical flavonol-type flavonoid and is present in a variety of vegetables, and their antioxidant effect implies their possible role in the prevention of oxidative stress related chronic diseases. Bone sialoprotein (BSP) is a noncollagenous protein of the extracellular matrix in the mineralized connective tissues that has been implicated in the nucleation of hydroxyapatite crystals. Previously, we reported that isoflavone (genistein) activated BSP gene transcription is mediated through an inverted CCAAT box in the proximal BSP gene promoter. The present study investigates the regulation of BSP transcription in a rat osteoblast-like cell line, ROS 17/2.8 cells, by quercetin and its conjugated metabolite quercetin 3-glucuronide. Quercetin and quercetin 3-glucuronide (5 microM) increased the BSP mRNA levels at 12 h and quercetin upregulated the Cbfa1/Runx2 mRNA expression at 12 h. From transient transfection assays using various sized BSP promoter-luciferase constructs, quercetin increased the luciferase activity of the construct (pLUC3), including the promoter sequence nucleotides -116 to -43. Transcriptional stimulations by quercetin were almost completely abrogated in the constructs that included 2 bp mutations in the inverted CCAAT and FRE elements whereas the CCAAT-protein complex did not change after stimulation by quercetin according to gel shift assays. Quercetin increased the nuclear protein binding to the FRE and 3'-FRE. These data suggest that quercetin and quercetin 3-glucuronide increased the BSP mRNA expression, and that the inverted CCAAT and FRE elements in the promoter of the BSP gene are required for quercetin induced BSP transcription.
Publication: J. Cell. Biochem. 2007 Jun 1 ;101(3)::790-800.
Publication Date: 2007 Jun 1
Study Author(s): Kim, Dong-Soon;Takai, Hideki;Arai, Masato;Araki, Shouta;Mezawa, Masaru;Kawai, Yoshichika;Murota, Kaeko;Terao, Junji;Ogata, Yorimasa;
Institution: Department of Periodontology, Nihon University School of Dentistry at Matsudo, Chiba 271-8587, Japan.
Shortcut link to this study:
Quercetin is a typical flavonol-type flavonoid and is present in a variety of vegetables, and their antioxidant effect implies their possible role in the prevention of oxidative stress related chronic diseases. Bone sialoprotein (BSP) is a noncollagenous protein of the extracellular matrix in the mineralized connective tissues that has been implicated in the nucleation of hydroxyapatite crystals. Previously, we reported that isoflavone (genistein) activated BSP gene transcription is mediated through an inverted CCAAT box in the proximal BSP gene promoter. The present study investigates the regulation of BSP transcription in a rat osteoblast-like cell line, ROS 17/2.8 cells, by quercetin and its conjugated metabolite quercetin 3-glucuronide. Quercetin and quercetin 3-glucuronide (5 microM) increased the BSP mRNA levels at 12 h and quercetin upregulated the Cbfa1/Runx2 mRNA expression at 12 h. From transient transfection assays using various sized BSP promoter-luciferase constructs, quercetin increased the luciferase activity of the construct (pLUC3), including the promoter sequence nucleotides -116 to -43. Transcriptional stimulations by quercetin were almost completely abrogated in the constructs that included 2 bp mutations in the inverted CCAAT and FRE elements whereas the CCAAT-protein complex did not change after stimulation by quercetin according to gel shift assays. Quercetin increased the nuclear protein binding to the FRE and 3'-FRE. These data suggest that quercetin and quercetin 3-glucuronide increased the BSP mRNA expression, and that the inverted CCAAT and FRE elements in the promoter of the BSP gene are required for quercetin induced BSP transcription.
PMID: 17243115