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result sql = Effects of genistein, resveratrol, and quercetin on steroidogenesis and proliferation of MA-10 mouse Leydig tumor cells.
Effects of genistein, resveratrol, and quercetin on steroidogenesis and proliferation of MA-10 mouse Leydig tumor cells.
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char = ;code = 32
char = I;code = 73
char = C;code = 67
char = I;code = 73
char = ;code = 32
char = 1;code = 49
char = 8;code = 56
char = 2;code = 50
char = ,;code = 44
char = 7;code = 55
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char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
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char = c;code = 99
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char = M;code = 77
char = P;code = 80
char = ;code = 32
char = p;code = 112
char = l;code = 108
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char = s;code = 115
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char = q;code = 113
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char = e;code = 101
char = r;code = 114
char = c;code = 99
char = e;code = 101
char = t;code = 116
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char = n;code = 110
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char = i;code = 105
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char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = S;code = 83
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char = A;code = 65
char = R;code = 82
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = o;code = 111
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char = v;code = 118
char = i;code = 105
char = t;code = 116
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char = T;code = 84
char = o;code = 111
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char = e;code = 101
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char = n;code = 110
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char = w;code = 119
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char = e;code = 101
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = r;code = 114
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char = n;code = 110
char = o;code = 111
char = n;code = 110
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char = E;code = 69
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char = f;code = 102
char = a;code = 97
char = c;code = 99
char = t;code = 116
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char = a;code = 97
char = r;code = 114
char = e;code = 101
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char = a;code = 97
char = s;code = 115
char = s;code = 115
char = o;code = 111
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char = i;code = 105
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = w;code = 119
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char = t;code = 116
char = h;code = 104
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char = q;code = 113
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char = e;code = 101
char = r;code = 114
char = c;code = 99
char = e;code = 101
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char = i;code = 105
char = n;code = 110
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char = s;code = 115
char = t;code = 116
char = i;code = 105
char = m;code = 109
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char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = p;code = 112
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char = o;code = 111
char = g;code = 103
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = r;code = 114
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char = n;code = 110
char = e;code = 101
char = ;code = 32
char = p;code = 112
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char = d;code = 100
char = u;code = 117
char = c;code = 99
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char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = w;code = 119
char = e;code = 101
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char = t;code = 116
char = r;code = 114
char = e;code = 101
char = a;code = 97
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = M;code = 77
char = A;code = 65
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char = 1;code = 49
char = 0;code = 48
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char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = E;code = 69
char = G;code = 71
char = T;code = 84
char = A;code = 65
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = p;code = 112
char = r;code = 114
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = m;code = 109
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = C;code = 67
char = a;code = 97
char = (;code = 40
char = 2;code = 50
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char = );code = 41
char = .;code = 46
char = ;code = 32
char = W;code = 87
char = e;code = 101
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char = f;code = 102
char = o;code = 111
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char = n;code = 110
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = E;code = 69
char = G;code = 71
char = T;code = 84
char = A;code = 65
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char = i;code = 105
char = n;code = 110
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char = i;code = 105
char = b;code = 98
char = i;code = 105
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char = d;code = 100
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char = q;code = 113
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char = e;code = 101
char = r;code = 114
char = c;code = 99
char = e;code = 101
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char = i;code = 105
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char = p;code = 112
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char = u;code = 117
char = s;code = 115
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char = t;code = 116
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char = o;code = 111
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char = a;code = 97
char = n;code = 110
char = d;code = 100
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char = S;code = 83
char = t;code = 116
char = A;code = 65
char = R;code = 82
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
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char = o;code = 111
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char = a;code = 97
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char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
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char = B;code = 66
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char = k;code = 107
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char = C;code = 67
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char = h;code = 104
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char = T;code = 84
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char = u;code = 117
char = s;code = 115
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char = c;code = 99
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char = P;code = 80
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char = i;code = 105
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char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = S;code = 83
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char = A;code = 65
char = R;code = 82
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = o;code = 111
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char = t;code = 116
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char = k;code = 107
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char = l;code = 108
char = u;code = 117
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char = S;code = 83
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char = p;code = 112
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char = o;code = 111
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char = l;code = 108
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char = E;code = 69
char = G;code = 71
char = T;code = 84
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char = P;code = 80
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char = t;code = 116
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char = R;code = 82
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char = m;code = 109
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char = N;code = 78
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char = e;code = 101
char = x;code = 120
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char = 1;code = 49
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result sql = This study was performed to compare the effects of three well-known phytoestrogens such as genistein, resveratrol, and quercetin on steroidogenesis in MA-10 mouse tumor Leydig cells. Addition of genistein or resveratrol to MA-10 cells resulted in decreases in the cAMP-stimulated progesterone secretion, but quercetin had an opposite response. Steroidogenic acute regulatory (StAR) mRNA expression and StAR promoter activity in transiently transfected MA-10 cells were significantly reduced by genistein or resveratrol, but increased by quercetin. Genistein was found to inhibit MA-10 cell proliferation, while resveratrol and quercetin had no effect. Quercetin-induced increase in cAMP-stimulated progesterone secretion was reversed by ICI 182,780, an estrogen receptor (ER) antagonist. However, ICI 182,780 had no effect on cAMP plus quercetin-stimulated StAR promoter activity. To examine whether non-ER factors are associated with quercetin-stimulated progesterone production, we treated MA-10 cells with EGTA to deprive them of extracellular Ca(2+). We found that EGTA inhibited quercetin-plus cAMP-stimulated progesterone secretion and StAR promoter activity. Blocking of Ca(2+) influx through L- or T-type voltage-gated Ca(2+) channels with verapamil or mibefradil respectively, attenuated quercetin-stimulated progesterone secretion, while they had no effect on quercetin-plus cAMP-stimulated StAR promoter activity. Blocking of intracellular Ca(2+) efflux by sodium orthovanadate, a Ca(2+)-pump inhibitor, blocked quercetin- plus cAMP-stimulated progesterone secretion and StAR promoter activity in MA-10 cells. Finally, EGTA or vanadate reduced quercetin and cAMP-increased in StAR mRNA expression in MA-10 cells, while ICI 182,780 had no effect. Taken together, these results indicate that phytoestrogens have differential effects on steroidogenesis in MA-10 cells.

Publication: J. Endocrinol. 2007 Mar ;192(3)::527-37.
Publication Date: 2007 Mar
Study Author(s): Chen, Yu-Chyu;Nagpal, Madan L;Stocco, Douglas M;Lin, Tu;
Institution: Medical and Research Services, WJB Dorn Veterans Medical Center, Columbia, South Carolina 29201, USA.
Shortcut link to this study:
This study was performed to compare the effects of three well-known phytoestrogens such as genistein, resveratrol, and quercetin on steroidogenesis in MA-10 mouse tumor Leydig cells. Addition of genistein or resveratrol to MA-10 cells resulted in decreases in the cAMP-stimulated progesterone secretion, but quercetin had an opposite response. Steroidogenic acute regulatory (StAR) mRNA expression and StAR promoter activity in transiently transfected MA-10 cells were significantly reduced by genistein or resveratrol, but increased by quercetin. Genistein was found to inhibit MA-10 cell proliferation, while resveratrol and quercetin had no effect. Quercetin-induced increase in cAMP-stimulated progesterone secretion was reversed by ICI 182,780, an estrogen receptor (ER) antagonist. However, ICI 182,780 had no effect on cAMP plus quercetin-stimulated StAR promoter activity. To examine whether non-ER factors are associated with quercetin-stimulated progesterone production, we treated MA-10 cells with EGTA to deprive them of extracellular Ca(2+). We found that EGTA inhibited quercetin-plus cAMP-stimulated progesterone secretion and StAR promoter activity. Blocking of Ca(2+) influx through L- or T-type voltage-gated Ca(2+) channels with verapamil or mibefradil respectively, attenuated quercetin-stimulated progesterone secretion, while they had no effect on quercetin-plus cAMP-stimulated StAR promoter activity. Blocking of intracellular Ca(2+) efflux by sodium orthovanadate, a Ca(2+)-pump inhibitor, blocked quercetin- plus cAMP-stimulated progesterone secretion and StAR promoter activity in MA-10 cells. Finally, EGTA or vanadate reduced quercetin and cAMP-increased in StAR mRNA expression in MA-10 cells, while ICI 182,780 had no effect. Taken together, these results indicate that phytoestrogens have differential effects on steroidogenesis in MA-10 cells.
PMID: 17332522

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