char = M;code = 77 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = h;code = 104 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = k;code = 107 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = -;code = 45 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = a;code = 97 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = (;code = 40 char = v;code = 118 char = );code = 41 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = .;code = 46 result sql = Mutation of human plasminogen kringle 1-5 enhances anti-angiogenic action via increased interaction with integrin alpha(v)beta(3).
Mutation of human plasminogen kringle 1-5 enhances anti-angiogenic action via increased interaction with integrin alpha(v)beta(3).char = A;code = 65 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = o;code = 111 char = l;code = 108 char = y;code = 121 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = r;code = 114 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = k;code = 107 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = h;code = 104 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = -;code = 45 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = k;code = 107 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = ;code = 32 char = (;code = 40 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = k;code = 107 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = 5;code = 53 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = o;code = 111 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = r;code = 114 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = ;code = 32 char = k;code = 107 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = -;code = 45 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = y;code = 121 char = c;code = 99 char = o;code = 111 char = s;code = 115 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = A;code = 65 char = s;code = 115 char = n;code = 110 char = -;code = 45 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = r;code = 114 char = -;code = 45 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = L;code = 76 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = L;code = 76 char = e;code = 101 char = u;code = 117 char = -;code = 45 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = k;code = 107 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = 5;code = 53 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = l;code = 108 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = ;code = 32 char = (;code = 40 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = A;code = 65 char = s;code = 115 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = A;code = 65 char = l;code = 108 char = a;code = 97 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = d;code = 100 char = u;code = 117 char = e;code = 101 char = ;code = 32 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = i;code = 105 char = l;code = 108 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = c;code = 99 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = P;code = 80 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = a;code = 97 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = .;code = 46 char = ;code = 32 char = N;code = 78 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = o;code = 111 char = l;code = 108 char = y;code = 121 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = v;code = 118 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = l;code = 108 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = b;code = 98 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = (;code = 40 char = v;code = 118 char = );code = 41 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = -;code = 45 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = d;code = 100 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = .;code = 46 char = ;code = 32 char = F;code = 70 char = u;code = 117 char = r;code = 114 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = b;code = 98 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = M;code = 77 char = a;code = 97 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = g;code = 103 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = v;code = 118 char = o;code = 111 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = j;code = 106 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = N;code = 78 char = 2;code = 50 char = 8;code = 56 char = 9;code = 57 char = A;code = 65 char = /;code = 47 char = T;code = 84 char = 3;code = 51 char = 4;code = 52 char = 6;code = 54 char = A;code = 65 char = /;code = 47 char = L;code = 76 char = 5;code = 53 char = 3;code = 51 char = 2;code = 50 char = R;code = 82 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = y;code = 121 char = c;code = 99 char = o;code = 111 char = s;code = 115 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = L;code = 76 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = -;code = 45 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = K;code = 75 char = (;code = 40 char = 1;code = 49 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = b;code = 98 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = a;code = 97 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = (;code = 40 char = v;code = 118 char = );code = 41 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Angiogenesis plays a primary role in tumor growth and metastasis. Angiostatin, a proteolytic fragment containing the first four kringle domains of human plasminogen, can inhibit angiogenesis. The anti-angiogenic activities of kringle 1-5 (K(1-5)) and kringle 5 fragments of plasminogen are greater than angiostatin in inhibiting angiogenesis and angiogenesis-dependent tumor growth. To further optimize kringle fragment anti-angiogenic activities, mutations were created at the potential glycosylation sites Asn-289 and Thr-346 and the Lys binding site, Leu-532, at kringle 5, including K(1-5)N289A (replacing Asn by Ala at residue 289), K(1-5)T346A, K(1-5)L532R, K(1-5)N289A/T346A, K(1-5)T346A/L532R, K(1-5)N289A/L532R, and K(1-5)N289A/T346A/L532R. Wild-type and mutant K(1-5) proteins were expressed successfully by the Pichia pastoris expression system. Native K(1-5) from proteolytic cleavage and wild-type K(1-5) have similar activity in inhibiting basic fibroblast growth factor-induced endothelial cell proliferation. Among these mutated proteins, K(1-5)N289A/T346A/L532R exhibited the greatest effect in inhibiting endothelial cell proliferation and in inducing endothelial cell apoptosis. Integrin alpha(v)beta(3)-mediated adhesion of K(1-5)N289A/T346A/L532R to endothelial cells was more greatly enhanced when compared to wild type K(1-5). Furthermore, K(1-5)N289A/T346A/L532R was most potent in inhibiting basic fibroblast growth factor-induced angiogenesis in Matrigel assay in vivo. Angiogenesis-dependent tumor growth was inhibited by systemically injected K(1-5)N289A/T346A/L532R into mice. These results demonstrate that alteration of glycosylation and Lys binding properties could increase the anti-angiogenic action of K(1-5), possibly via enhanced interaction with integrin alpha(v)beta(3) in endothelial cells.
Publication: Thromb. Haemost. 2008 Apr ;99(4)::729-38. :doi: 10.1160/TH07-06-0403.
Publication Date: 2008 Apr
Study Author(s): Chang, Po-Chiao;Chang, Yu-Jia;Wu, Hua-Lin;Chang, Chin-Wei;Lin, Chung-I;Wang, Wei-Chih;Shi, Guey-Yueh;
Institution: Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, No. 1, University Rd, Tainan, Taiwan, 701.
Shortcut link to this study:
Angiogenesis plays a primary role in tumor growth and metastasis. Angiostatin, a proteolytic fragment containing the first four kringle domains of human plasminogen, can inhibit angiogenesis. The anti-angiogenic activities of kringle 1-5 (K(1-5)) and kringle 5 fragments of plasminogen are greater than angiostatin in inhibiting angiogenesis and angiogenesis-dependent tumor growth. To further optimize kringle fragment anti-angiogenic activities, mutations were created at the potential glycosylation sites Asn-289 and Thr-346 and the Lys binding site, Leu-532, at kringle 5, including K(1-5)N289A (replacing Asn by Ala at residue 289), K(1-5)T346A, K(1-5)L532R, K(1-5)N289A/T346A, K(1-5)T346A/L532R, K(1-5)N289A/L532R, and K(1-5)N289A/T346A/L532R. Wild-type and mutant K(1-5) proteins were expressed successfully by the Pichia pastoris expression system. Native K(1-5) from proteolytic cleavage and wild-type K(1-5) have similar activity in inhibiting basic fibroblast growth factor-induced endothelial cell proliferation. Among these mutated proteins, K(1-5)N289A/T346A/L532R exhibited the greatest effect in inhibiting endothelial cell proliferation and in inducing endothelial cell apoptosis. Integrin alpha(v)beta(3)-mediated adhesion of K(1-5)N289A/T346A/L532R to endothelial cells was more greatly enhanced when compared to wild type K(1-5). Furthermore, K(1-5)N289A/T346A/L532R was most potent in inhibiting basic fibroblast growth factor-induced angiogenesis in Matrigel assay in vivo. Angiogenesis-dependent tumor growth was inhibited by systemically injected K(1-5)N289A/T346A/L532R into mice. These results demonstrate that alteration of glycosylation and Lys binding properties could increase the anti-angiogenic action of K(1-5), possibly via enhanced interaction with integrin alpha(v)beta(3) in endothelial cells.
PMID: 18392331