|
|
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result sql = [Preparation of iodine-125 labeled anti-human CD40 monoclonal antibody 5H6 and its binding properties in ovary cancer cell line HO8910 in vitro].
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char = 9;code = 57
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char = c;code = 99
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char = l;code = 108
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char = e;code = 101
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char = s;code = 115
char = t;code = 116
char = u;code = 117
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char = i;code = 105
char = e;code = 101
char = d;code = 100
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char = l;code = 108
char = l;code = 108
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char = b;code = 98
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char = n;code = 110
char = d;code = 100
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char = n;code = 110
char = g;code = 103
char = ;code = 32
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char = p;code = 112
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = m;code = 109
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char = n;code = 110
char = t;code = 116
char = s;code = 115
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char = <;code = 60
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char = r;code = 114
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char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = R;code = 82
char = E;code = 69
char = S;code = 83
char = U;code = 85
char = L;code = 76
char = T;code = 84
char = S;code = 83
char = ;code = 32
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
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char = 1;code = 49
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char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = l;code = 108
char = a;code = 97
char = b;code = 98
char = e;code = 101
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char = i;code = 105
char = n;code = 110
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char = e;code = 101
char = f;code = 102
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = i;code = 105
char = e;code = 101
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char = c;code = 99
char = y;code = 121
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
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char = I;code = 73
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char = 5;code = 53
char = H;code = 72
char = 6;code = 54
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char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = (;code = 40
char = 8;code = 56
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char = .;code = 46
char = 4;code = 52
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char = 2;code = 50
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char = %;code = 37
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char = a;code = 97
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char = ;code = 32
char = i;code = 105
char = t;code = 116
char = s;code = 115
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char = r;code = 114
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = o;code = 111
char = c;code = 99
char = h;code = 104
char = e;code = 101
char = m;code = 109
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char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = p;code = 112
char = u;code = 117
char = r;code = 114
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 9;code = 57
char = 9;code = 57
char = .;code = 46
char = 2;code = 50
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = 0;code = 48
char = .;code = 46
char = 5;code = 53
char = );code = 41
char = %;code = 37
char = .;code = 46
char = ;code = 32
char = (;code = 40
char = 2;code = 50
char = );code = 41
char = R;code = 82
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = o;code = 111
char = c;code = 99
char = h;code = 104
char = e;code = 101
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char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = p;code = 112
char = u;code = 117
char = r;code = 114
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 8;code = 56
char = 0;code = 48
char = .;code = 46
char = 3;code = 51
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = 4;code = 52
char = .;code = 46
char = 7;code = 55
char = );code = 41
char = %;code = 37
char = ;code = 32
char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = 7;code = 55
char = ;code = 32
char = d;code = 100
char = a;code = 97
char = y;code = 121
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = 4;code = 52
char = ;code = 32
char = D;code = 68
char = e;code = 101
char = g;code = 103
char = r;code = 114
char = e;code = 101
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = C;code = 67
char = e;code = 101
char = l;code = 108
char = s;code = 115
char = i;code = 105
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = P;code = 80
char = B;code = 66
char = ;code = 32
char = (;code = 40
char = p;code = 112
char = h;code = 104
char = o;code = 111
char = s;code = 115
char = p;code = 112
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = b;code = 98
char = u;code = 117
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = r;code = 114
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char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = (;code = 40
char = 9;code = 57
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char = .;code = 46
char = 3;code = 51
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = 0;code = 48
char = .;code = 46
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char = );code = 41
char = %;code = 37
char = ;code = 32
char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = 2;code = 50
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char = ;code = 32
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char = u;code = 117
char = r;code = 114
char = s;code = 115
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char = i;code = 105
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char = D;code = 68
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char = g;code = 103
char = r;code = 114
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char = e;code = 101
char = s;code = 115
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char = C;code = 67
char = e;code = 101
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char = l;code = 108
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char = u;code = 117
char = s;code = 115
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char = a;code = 97
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char = m;code = 109
char = a;code = 97
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char = (;code = 40
char = 3;code = 51
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char = A;code = 65
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char = 4;code = 52
char = ;code = 32
char = D;code = 68
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char = e;code = 101
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char = C;code = 67
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char = i;code = 105
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char = r;code = 114
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char = 1;code = 49
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char = I;code = 73
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char = H;code = 72
char = 6;code = 54
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char = H;code = 72
char = O;code = 79
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char = l;code = 108
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char = w;code = 119
char = a;code = 97
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char = 7;code = 55
char = 1;code = 49
char = 1;code = 49
char = +;code = 43
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char = n;code = 110
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char = L;code = 76
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char = a;code = 97
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char = d;code = 100
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char = B;code = 66
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char = (;code = 40
char = 2;code = 50
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char = 1;code = 49
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char = +;code = 43
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char = .;code = 46
char = 0;code = 48
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char = c;code = 99
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char = I;code = 73
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char = a;code = 97
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char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
char = y;code = 121
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char = f;code = 102
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char = 1;code = 49
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char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 3;code = 51
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char = .;code = 46
char = 6;code = 54
char = +;code = 43
char = /;code = 47
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char = 4;code = 52
char = );code = 41
char = %;code = 37
char = .;code = 46
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char = (;code = 40
char = 5;code = 53
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char = R;code = 82
char = e;code = 101
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char = e;code = 101
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char = o;code = 111
char = f;code = 102
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char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
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char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = H;code = 72
char = O;code = 79
char = 8;code = 56
char = 9;code = 57
char = 1;code = 49
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
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char = w;code = 119
char = a;code = 97
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char = (;code = 40
char = 8;code = 56
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char = 8;code = 56
char = +;code = 43
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char = 6;code = 54
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char = %;code = 37
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char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
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char = r;code = 114
char = s;code = 115
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char = a;code = 97
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char = ;code = 32
char = 4;code = 52
char = ;code = 32
char = D;code = 68
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char = g;code = 103
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char = e;code = 101
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char = u;code = 117
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char = a;code = 97
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char = u;code = 117
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char = <;code = 60
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char = <;code = 60
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char = >;code = 62
char = <;code = 60
char = b;code = 98
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char = C;code = 67
char = O;code = 79
char = N;code = 78
char = C;code = 67
char = L;code = 76
char = U;code = 85
char = S;code = 83
char = I;code = 73
char = O;code = 79
char = N;code = 78
char = ;code = 32
char = :;code = 58
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char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = (;code = 40
char = 1;code = 49
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char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
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char = p;code = 112
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char = s;code = 115
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char = s;code = 115
char = s;code = 115
char = e;code = 101
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char = m;code = 109
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char = c;code = 99
char = a;code = 97
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char = u;code = 117
char = r;code = 114
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = .;code = 46
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = s;code = 115
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char = s;code = 115
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char = i;code = 105
char = t;code = 116
char = a;code = 97
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char = a;code = 97
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char = t;code = 116
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char = a;code = 97
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char = i;code = 105
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char = i;code = 105
char = m;code = 109
char = m;code = 109
char = u;code = 117
char = n;code = 110
char = o;code = 111
char = r;code = 114
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result sql = AIM : To prepare iodine-125 labeled anti-human CD40 monoclonal antibody 5H6 ((125)I-5H6) and investigate the binding properties of (125)I-5H6 to HO8910 cells in vitro.
METHODS : The mAb 5H6 was labeled with Na (125)I using chloramine-T method. The labeling efficiency and radiochemical purity of (125)I-5H6 were measured by paper chromatography. The stability of (125)I-5H6 in vitro was monitored by trichloroacetic acid (TCA) precipitation. The dissociation constant (K(d)) of (125)I-5H6 from HO8910 cells and the numbers of maximum binding sites (B(max)) were obtained by Scatchard analysis. Immunoreactivity of (125)I-5H6 to HO8910 cells was determined by "Lindmo" assay and its improved method. Internalization and retention of (125)I- 5H6 by HO8910 cells were studied by cell binding experiments.
RESULTS : (1)The labeling efficiency of (125)I-5H6 was (85.4+/-5.2)% and its radiochemical purity was (99.2+/-0.5)%. (2)Radiochemical purity of (125)I-5H6 was (80.3+/-4.7)% after 7 days in 4 Degrees Celsius PB (phosphate buffer) and (95.3+/-0.8)% after 24 hours in 37 Degrees Ceslius plasma. (3)At 4 Degrees Celsius, K(d) for (125)I-5H6 to HO8910 cells was (0.711+/-0.06) nmol/L and Bmax was (2.17+/-0.08)x10(5) sites/cell. (4)Immunoreactivity of (125)I-5H6 was (38.6+/-5.4)%. (5)Retention rate of (125)I-5H6 with HO8910 cells was (89.8+/-6.0)% after 2 hours at 4 Degrees Celsius and internalization rate of (125)I-5H6 by HO8910 cells was (54.9+/-2.6)% after 2 hours at 37 Degrees Celsius.
CONCLUSION : (125)I-5H6 possesses good labeling efficiency and perfect radiochemical purity. (125)I-5H6 has suitable stability in vitro and its immunoreactivity was not low. The high affinity of (125)I-5H6 with HO8910 cells will benefit further animal study in vivo.
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result sql = [Preparation of iodine-125 labeled anti-human CD40 monoclonal antibody 5H6 and its binding properties in ovary cancer cell line HO8910 in vitro].
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char = t;code = 116
char = c;code = 99
char = h;code = 104
char = a;code = 97
char = r;code = 114
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = y;code = 121
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = I;code = 73
char = m;code = 109
char = m;code = 109
char = u;code = 117
char = n;code = 110
char = o;code = 111
char = r;code = 114
char = e;code = 101
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
char = y;code = 121
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = H;code = 72
char = O;code = 79
char = 8;code = 56
char = 9;code = 57
char = 1;code = 49
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = ";code = 34
char = L;code = 76
char = i;code = 105
char = n;code = 110
char = d;code = 100
char = m;code = 109
char = o;code = 111
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char = ;code = 32
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = a;code = 97
char = y;code = 121
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = m;code = 109
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = v;code = 118
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = m;code = 109
char = e;code = 101
char = t;code = 116
char = h;code = 104
char = o;code = 111
char = d;code = 100
char = .;code = 46
char = ;code = 32
char = I;code = 73
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = i;code = 105
char = z;code = 122
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = t;code = 116
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = ;code = 32
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = H;code = 72
char = O;code = 79
char = 8;code = 56
char = 9;code = 57
char = 1;code = 49
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = u;code = 117
char = d;code = 100
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = b;code = 98
char = i;code = 105
char = n;code = 110
char = d;code = 100
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = p;code = 112
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = m;code = 109
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = s;code = 115
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = R;code = 82
char = E;code = 69
char = S;code = 83
char = U;code = 85
char = L;code = 76
char = T;code = 84
char = S;code = 83
char = ;code = 32
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = (;code = 40
char = 1;code = 49
char = );code = 41
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = l;code = 108
char = a;code = 97
char = b;code = 98
char = e;code = 101
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = e;code = 101
char = f;code = 102
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = i;code = 105
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 8;code = 56
char = 5;code = 53
char = .;code = 46
char = 4;code = 52
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = 5;code = 53
char = .;code = 46
char = 2;code = 50
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char = %;code = 37
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = t;code = 116
char = s;code = 115
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char = r;code = 114
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = o;code = 111
char = c;code = 99
char = h;code = 104
char = e;code = 101
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = p;code = 112
char = u;code = 117
char = r;code = 114
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 9;code = 57
char = 9;code = 57
char = .;code = 46
char = 2;code = 50
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = 0;code = 48
char = .;code = 46
char = 5;code = 53
char = );code = 41
char = %;code = 37
char = .;code = 46
char = ;code = 32
char = (;code = 40
char = 2;code = 50
char = );code = 41
char = R;code = 82
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = o;code = 111
char = c;code = 99
char = h;code = 104
char = e;code = 101
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = p;code = 112
char = u;code = 117
char = r;code = 114
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = );code = 41
char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 8;code = 56
char = 0;code = 48
char = .;code = 46
char = 3;code = 51
char = +;code = 43
char = /;code = 47
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char = 4;code = 52
char = .;code = 46
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char = %;code = 37
char = ;code = 32
char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
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char = 7;code = 55
char = ;code = 32
char = d;code = 100
char = a;code = 97
char = y;code = 121
char = s;code = 115
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char = i;code = 105
char = n;code = 110
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char = 4;code = 52
char = ;code = 32
char = D;code = 68
char = e;code = 101
char = g;code = 103
char = r;code = 114
char = e;code = 101
char = e;code = 101
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char = C;code = 67
char = e;code = 101
char = l;code = 108
char = s;code = 115
char = i;code = 105
char = u;code = 117
char = s;code = 115
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char = P;code = 80
char = B;code = 66
char = ;code = 32
char = (;code = 40
char = p;code = 112
char = h;code = 104
char = o;code = 111
char = s;code = 115
char = p;code = 112
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = b;code = 98
char = u;code = 117
char = f;code = 102
char = f;code = 102
char = e;code = 101
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char = ;code = 32
char = a;code = 97
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char = d;code = 100
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char = 9;code = 57
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char = .;code = 46
char = 3;code = 51
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char = /;code = 47
char = -;code = 45
char = 0;code = 48
char = .;code = 46
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char = );code = 41
char = %;code = 37
char = ;code = 32
char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
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char = 2;code = 50
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char = ;code = 32
char = h;code = 104
char = o;code = 111
char = u;code = 117
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = i;code = 105
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char = 3;code = 51
char = 7;code = 55
char = ;code = 32
char = D;code = 68
char = e;code = 101
char = g;code = 103
char = r;code = 114
char = e;code = 101
char = e;code = 101
char = s;code = 115
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char = C;code = 67
char = e;code = 101
char = s;code = 115
char = l;code = 108
char = i;code = 105
char = u;code = 117
char = s;code = 115
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char = l;code = 108
char = a;code = 97
char = s;code = 115
char = m;code = 109
char = a;code = 97
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char = ;code = 32
char = (;code = 40
char = 3;code = 51
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char = A;code = 65
char = t;code = 116
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char = 4;code = 52
char = ;code = 32
char = D;code = 68
char = e;code = 101
char = g;code = 103
char = r;code = 114
char = e;code = 101
char = e;code = 101
char = s;code = 115
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char = C;code = 67
char = e;code = 101
char = l;code = 108
char = s;code = 115
char = i;code = 105
char = u;code = 117
char = s;code = 115
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char = ;code = 32
char = K;code = 75
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char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
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char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = H;code = 72
char = O;code = 79
char = 8;code = 56
char = 9;code = 57
char = 1;code = 49
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 0;code = 48
char = .;code = 46
char = 7;code = 55
char = 1;code = 49
char = 1;code = 49
char = +;code = 43
char = /;code = 47
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char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 6;code = 54
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char = ;code = 32
char = n;code = 110
char = m;code = 109
char = o;code = 111
char = l;code = 108
char = /;code = 47
char = L;code = 76
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = B;code = 66
char = m;code = 109
char = a;code = 97
char = x;code = 120
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = (;code = 40
char = 2;code = 50
char = .;code = 46
char = 1;code = 49
char = 7;code = 55
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 8;code = 56
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char = x;code = 120
char = 1;code = 49
char = 0;code = 48
char = (;code = 40
char = 5;code = 53
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char = ;code = 32
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char = i;code = 105
char = t;code = 116
char = e;code = 101
char = s;code = 115
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char = c;code = 99
char = e;code = 101
char = l;code = 108
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char = .;code = 46
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char = (;code = 40
char = 4;code = 52
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char = I;code = 73
char = m;code = 109
char = m;code = 109
char = u;code = 117
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char = r;code = 114
char = e;code = 101
char = a;code = 97
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char = t;code = 116
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char = v;code = 118
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char = y;code = 121
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char = f;code = 102
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char = I;code = 73
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char = H;code = 72
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char = (;code = 40
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char = );code = 41
char = %;code = 37
char = .;code = 46
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char = R;code = 82
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char = I;code = 73
char = -;code = 45
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char = H;code = 72
char = 6;code = 54
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char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = H;code = 72
char = O;code = 79
char = 8;code = 56
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char = 1;code = 49
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
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char = a;code = 97
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char = (;code = 40
char = 8;code = 56
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char = .;code = 46
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char = +;code = 43
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char = %;code = 37
char = ;code = 32
char = a;code = 97
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char = t;code = 116
char = e;code = 101
char = r;code = 114
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char = h;code = 104
char = o;code = 111
char = u;code = 117
char = r;code = 114
char = s;code = 115
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char = a;code = 97
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char = ;code = 32
char = 4;code = 52
char = ;code = 32
char = D;code = 68
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char = g;code = 103
char = r;code = 114
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char = e;code = 101
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char = C;code = 67
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char = u;code = 117
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char = i;code = 105
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char = e;code = 101
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char = a;code = 97
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char = i;code = 105
char = z;code = 122
char = a;code = 97
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char = o;code = 111
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char = a;code = 97
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char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
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char = I;code = 73
char = -;code = 45
char = 5;code = 53
char = H;code = 72
char = 6;code = 54
char = ;code = 32
char = b;code = 98
char = y;code = 121
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char = H;code = 72
char = O;code = 79
char = 8;code = 56
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char = 1;code = 49
char = 0;code = 48
char = ;code = 32
char = c;code = 99
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char = l;code = 108
char = s;code = 115
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 5;code = 53
char = 4;code = 52
char = .;code = 46
char = 9;code = 57
char = +;code = 43
char = /;code = 47
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char = 2;code = 50
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char = 6;code = 54
char = );code = 41
char = %;code = 37
char = ;code = 32
char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
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char = 2;code = 50
char = ;code = 32
char = h;code = 104
char = o;code = 111
char = u;code = 117
char = r;code = 114
char = s;code = 115
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char = a;code = 97
char = t;code = 116
char = ;code = 32
char = 3;code = 51
char = 7;code = 55
char = ;code = 32
char = D;code = 68
char = e;code = 101
char = g;code = 103
char = r;code = 114
char = e;code = 101
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = C;code = 67
char = e;code = 101
char = l;code = 108
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result sql = AIM : To prepare iodine-125 labeled anti-human CD40 monoclonal antibody 5H6 ((125)I-5H6) and investigate the binding properties of (125)I-5H6 to HO8910 cells in vitro.
METHODS : The mAb 5H6 was labeled with Na (125)I using chloramine-T method. The labeling efficiency and radiochemical purity of (125)I-5H6 were measured by paper chromatography. The stability of (125)I-5H6 in vitro was monitored by trichloroacetic acid (TCA) precipitation. The dissociation constant (K(d)) of (125)I-5H6 from HO8910 cells and the numbers of maximum binding sites (B(max)) were obtained by Scatchard analysis. Immunoreactivity of (125)I-5H6 to HO8910 cells was determined by "Lindmo" assay and its improved method. Internalization and retention of (125)I- 5H6 by HO8910 cells were studied by cell binding experiments.
RESULTS : (1)The labeling efficiency of (125)I-5H6 was (85.4+/-5.2)% and its radiochemical purity was (99.2+/-0.5)%. (2)Radiochemical purity of (125)I-5H6 was (80.3+/-4.7)% after 7 days in 4 Degrees Celsius PB (phosphate buffer) and (95.3+/-0.8)% after 24 hours in 37 Degrees Ceslius plasma. (3)At 4 Degrees Celsius, K(d) for (125)I-5H6 to HO8910 cells was (0.711+/-0.06) nmol/L and Bmax was (2.17+/-0.08)x10(5) sites/cell. (4)Immunoreactivity of (125)I-5H6 was (38.6+/-5.4)%. (5)Retention rate of (125)I-5H6 with HO8910 cells was (89.8+/-6.0)% after 2 hours at 4 Degrees Celsius and internalization rate of (125)I-5H6 by HO8910 cells was (54.9+/-2.6)% after 2 hours at 37 Degrees Celsius.
CONCLUSION : (125)I-5H6 possesses good labeling efficiency and perfect radiochemical purity. (125)I-5H6 has suitable stability in vitro and its immunoreactivity was not low. The high affinity of (125)I-5H6 with HO8910 cells will benefit further animal study in vivo.
Publication: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2008 Jun ;24(6)::590-3.
Publication Date: 2008 Jun
Study Author(s): Luo, Xian-fu;Zhang, Bin;Ma, Hong-bing;Tang, Lin;Zhou, Xuan;Xu, Ying;Wu, Yi-wei;Zhang, Xue-guang;
Institution: Institute of Medical Biotechnology, Soochow University, Suzhou, China. [email protected]
Shortcut link to this study:
Publication Date: 2008 Jun
Study Author(s): Luo, Xian-fu;Zhang, Bin;Ma, Hong-bing;Tang, Lin;Zhou, Xuan;Xu, Ying;Wu, Yi-wei;Zhang, Xue-guang;
Institution: Institute of Medical Biotechnology, Soochow University, Suzhou, China. [email protected]
Shortcut link to this study:
AIM : To prepare iodine-125 labeled anti-human CD40 monoclonal antibody 5H6 ((125)I-5H6) and investigate the binding properties of (125)I-5H6 to HO8910 cells in vitro.
METHODS : The mAb 5H6 was labeled with Na (125)I using chloramine-T method. The labeling efficiency and radiochemical purity of (125)I-5H6 were measured by paper chromatography. The stability of (125)I-5H6 in vitro was monitored by trichloroacetic acid (TCA) precipitation. The dissociation constant (K(d)) of (125)I-5H6 from HO8910 cells and the numbers of maximum binding sites (B(max)) were obtained by Scatchard analysis. Immunoreactivity of (125)I-5H6 to HO8910 cells was determined by "Lindmo" assay and its improved method. Internalization and retention of (125)I- 5H6 by HO8910 cells were studied by cell binding experiments.
RESULTS : (1)The labeling efficiency of (125)I-5H6 was (85.4+/-5.2)% and its radiochemical purity was (99.2+/-0.5)%. (2)Radiochemical purity of (125)I-5H6 was (80.3+/-4.7)% after 7 days in 4 Degrees Celsius PB (phosphate buffer) and (95.3+/-0.8)% after 24 hours in 37 Degrees Ceslius plasma. (3)At 4 Degrees Celsius, K(d) for (125)I-5H6 to HO8910 cells was (0.711+/-0.06) nmol/L and Bmax was (2.17+/-0.08)x10(5) sites/cell. (4)Immunoreactivity of (125)I-5H6 was (38.6+/-5.4)%. (5)Retention rate of (125)I-5H6 with HO8910 cells was (89.8+/-6.0)% after 2 hours at 4 Degrees Celsius and internalization rate of (125)I-5H6 by HO8910 cells was (54.9+/-2.6)% after 2 hours at 37 Degrees Celsius.
CONCLUSION : (125)I-5H6 possesses good labeling efficiency and perfect radiochemical purity. (125)I-5H6 has suitable stability in vitro and its immunoreactivity was not low. The high affinity of (125)I-5H6 with HO8910 cells will benefit further animal study in vivo.
METHODS : The mAb 5H6 was labeled with Na (125)I using chloramine-T method. The labeling efficiency and radiochemical purity of (125)I-5H6 were measured by paper chromatography. The stability of (125)I-5H6 in vitro was monitored by trichloroacetic acid (TCA) precipitation. The dissociation constant (K(d)) of (125)I-5H6 from HO8910 cells and the numbers of maximum binding sites (B(max)) were obtained by Scatchard analysis. Immunoreactivity of (125)I-5H6 to HO8910 cells was determined by "Lindmo" assay and its improved method. Internalization and retention of (125)I- 5H6 by HO8910 cells were studied by cell binding experiments.
RESULTS : (1)The labeling efficiency of (125)I-5H6 was (85.4+/-5.2)% and its radiochemical purity was (99.2+/-0.5)%. (2)Radiochemical purity of (125)I-5H6 was (80.3+/-4.7)% after 7 days in 4 Degrees Celsius PB (phosphate buffer) and (95.3+/-0.8)% after 24 hours in 37 Degrees Ceslius plasma. (3)At 4 Degrees Celsius, K(d) for (125)I-5H6 to HO8910 cells was (0.711+/-0.06) nmol/L and Bmax was (2.17+/-0.08)x10(5) sites/cell. (4)Immunoreactivity of (125)I-5H6 was (38.6+/-5.4)%. (5)Retention rate of (125)I-5H6 with HO8910 cells was (89.8+/-6.0)% after 2 hours at 4 Degrees Celsius and internalization rate of (125)I-5H6 by HO8910 cells was (54.9+/-2.6)% after 2 hours at 37 Degrees Celsius.
CONCLUSION : (125)I-5H6 possesses good labeling efficiency and perfect radiochemical purity. (125)I-5H6 has suitable stability in vitro and its immunoreactivity was not low. The high affinity of (125)I-5H6 with HO8910 cells will benefit further animal study in vivo.
PMID: 18538090
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