char = P;code = 80 char = g;code = 103 char = t;code = 116 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = S;code = 83 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = z;code = 122 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = y;code = 121 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = .;code = 46 result sql = Pgt1, a glutathione transporter from the fission yeast Schizosaccharomyces pombe.
Pgt1, a glutathione transporter from the fission yeast Schizosaccharomyces pombe.char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = z;code = 122 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = y;code = 121 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = O;code = 79 char = R;code = 82 char = F;code = 70 char = ,;code = 44 char = ;code = 32 char = S;code = 83 char = P;code = 80 char = A;code = 65 char = C;code = 67 char = 2;code = 50 char = 9;code = 57 char = B;code = 66 char = 1;code = 49 char = 2;code = 50 char = .;code = 46 char = 1;code = 49 char = 0;code = 48 char = c;code = 99 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = g;code = 103 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = (;code = 40 char = O;code = 79 char = P;code = 80 char = T;code = 84 char = );code = 41 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = l;code = 108 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = .;code = 46 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = (;code = 40 char = P;code = 80 char = g;code = 103 char = t;code = 116 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = l;code = 108 char = o;code = 111 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = a;code = 97 char = D;code = 68 char = e;code = 101 char = l;code = 108 char = t;code = 116 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = .;code = 46 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = s;code = 115 char = y;code = 121 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = );code = 41 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = e;code = 101 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = .;code = 46 char = ;code = 32 char = D;code = 68 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = O;code = 79 char = R;code = 82 char = F;code = 70 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = a;code = 97 char = D;code = 68 char = e;code = 101 char = l;code = 108 char = t;code = 116 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = u;code = 117 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = O;code = 79 char = R;code = 82 char = F;code = 70 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = a;code = 97 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = -;code = 45 char = a;code = 97 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = K;code = 75 char = (;code = 40 char = m;code = 109 char = );code = 41 char = =;code = 61 char = 6;code = 54 char = 3;code = 51 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = M;code = 77 char = );code = 41 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = m;code = 109 char = a;code = 97 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = e;code = 101 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = j;code = 106 char = u;code = 117 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = p;code = 112 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = F;code = 70 char = u;code = 117 char = r;code = 114 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = -;code = 45 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = o;code = 111 char = g;code = 103 char = u;code = 117 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = g;code = 103 char = a;code = 97 char = m;code = 109 char = m;code = 109 char = a;code = 97 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = -;code = 45 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = -;code = 45 char = g;code = 103 char = l;code = 108 char = y;code = 121 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = t;code = 116 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = u;code = 117 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = w;code = 119 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = P;code = 80 char = g;code = 103 char = t;code = 116 char = 1;code = 49 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = r;code = 114 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = g;code = 103 char = t;code = 116 char = 1;code = 49 char = (;code = 40 char = +;code = 43 char = );code = 41 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = c;code = 99 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = v;code = 118 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = The Schizosaccharomyces pombe ORF, SPAC29B12.10c, a predicted member of the oligopeptide transporter (OPT) family, was identified as a gene encoding the S. pombe glutathione transporter (Pgt1) by a genetic strategy that exploited the requirement of the cys1aDelta strain of S. pombe (which is defective in cysteine biosynthesis) for either cysteine or glutathione, for growth. Disruption of the ORF in the cys1aDelta strain led to an inability to grow on glutathione as a source of cysteine. Cloning and subsequent biochemical characterization of the ORF revealed that a high-affinity transporter for glutathione (K(m)=63 microM) that was found to be localized to the plasma membrane. The transporter was specific for glutathione, as significant inhibition in glutathione uptake could be observed only by either reduced or oxidized glutathione, or glutathione conjugates, but not by dipeptides or tripeptides. Furthermore, although glu-cys-gly, an analogue of glutathione (gamma-glu-cys-gly), could be utilized as a sulphur source, the growth was not Pgt1 dependent. This further underlined the specificity of this transporter for glutathione. The strong repression of pgt1(+) expression by cysteine suggested a role in scavenging glutathione from the extracellular environment for the maintenance of sulphur homeostasis in this yeast.
Publication: FEMS Yeast Res. 2008 Sep ;8(6)::916-29. :doi: 10.1111/j.1567-1364.2008.00423.x.
Publication Date: 2008 Sep
Study Author(s): Thakur, Anil;Kaur, Jaspreet;Bachhawat, Anand K;
Institution: Institute of Microbial Technology, Chandigarh, India.
Shortcut link to this study:
The Schizosaccharomyces pombe ORF, SPAC29B12.10c, a predicted member of the oligopeptide transporter (OPT) family, was identified as a gene encoding the S. pombe glutathione transporter (Pgt1) by a genetic strategy that exploited the requirement of the cys1aDelta strain of S. pombe (which is defective in cysteine biosynthesis) for either cysteine or glutathione, for growth. Disruption of the ORF in the cys1aDelta strain led to an inability to grow on glutathione as a source of cysteine. Cloning and subsequent biochemical characterization of the ORF revealed that a high-affinity transporter for glutathione (K(m)=63 microM) that was found to be localized to the plasma membrane. The transporter was specific for glutathione, as significant inhibition in glutathione uptake could be observed only by either reduced or oxidized glutathione, or glutathione conjugates, but not by dipeptides or tripeptides. Furthermore, although glu-cys-gly, an analogue of glutathione (gamma-glu-cys-gly), could be utilized as a sulphur source, the growth was not Pgt1 dependent. This further underlined the specificity of this transporter for glutathione. The strong repression of pgt1(+) expression by cysteine suggested a role in scavenging glutathione from the extracellular environment for the maintenance of sulphur homeostasis in this yeast.
PMID: 18662319