char = [;code = 91 char = C;code = 67 char = h;code = 104 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = g;code = 103 char = r;code = 114 char = a;code = 97 char = p;code = 112 char = h;code = 104 char = y;code = 121 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ];code = 93 char = .;code = 46 result sql = [Chiral separation of bisoprolol using high performance liquid chromatography with amylose chiral stationary phase].
[Chiral separation of bisoprolol using high performance liquid chromatography with amylose chiral stationary phase].char = A;code = 65 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = g;code = 103 char = r;code = 114 char = a;code = 97 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = (;code = 40 char = H;code = 72 char = P;code = 80 char = L;code = 76 char = C;code = 67 char = );code = 41 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = d;code = 100 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = r;code = 114 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = 3;code = 51 char = ,;code = 44 char = 5;code = 53 char = -;code = 45 char = d;code = 100 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = b;code = 98 char = a;code = 97 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = );code = 41 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = l;code = 108 char = -;code = 45 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = g;code = 103 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = -;code = 45 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = l;code = 108 char = u;code = 117 char = m;code = 109 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = h;code = 104 char = e;code = 101 char = x;code = 120 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = -;code = 45 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = -;code = 45 char = d;code = 100 char = i;code = 105 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = 8;code = 56 char = 8;code = 56 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = 1;code = 49 char = 2;code = 50 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = v;code = 118 char = /;code = 47 char = v;code = 118 char = /;code = 47 char = v;code = 118 char = );code = 41 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = 2;code = 50 char = 0;code = 48 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = C;code = 67 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 6;code = 54 char = ;code = 32 char = m;code = 109 char = L;code = 76 char = /;code = 47 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = n;code = 110 char = g;code = 103 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = 2;code = 50 char = 7;code = 55 char = 0;code = 48 char = ;code = 32 char = n;code = 110 char = m;code = 109 char = .;code = 46 char = ;code = 32 char = B;code = 66 char = y;code = 121 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = c;code = 99 char = k;code = 107 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = l;code = 108 char = y;code = 121 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = A high performance liquid chromatographic (HPLC) method using amylase chiral stationary phase for the separation of bisoprolol enantiomers has been developed. Tris (3,5-dimethylphenyl carbamate) amylase chiral stationary phase was used in the normal-phase mode. The effects of the concentration of organic solvents (iso-propanol and ethanol), flow rate and column temperature on the enantiomer separation were studied systematically. The best separation was obtained with hexane-ethanol-diethyl amine (88 : 12 : 0.1, v/v/v) as mobile phase at 20 degrees C. The optimal flow rate was 0.6 mL/min. The detection wavelength was set at 270 nm. By using this method, the enantiomers of bisoprolol can be separated quickly and easily.
Publication: Se Pu 2008 Nov ;26(6)::766-8.
Publication Date: 2008 Nov
Study Author(s): Li, Fang;Li, Jiayang;Zhang, Huayan;Guo, Xingjie;
Institution: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
[email protected]
Shortcut link to this study:
A high performance liquid chromatographic (HPLC) method using amylase chiral stationary phase for the separation of bisoprolol enantiomers has been developed. Tris (3,5-dimethylphenyl carbamate) amylase chiral stationary phase was used in the normal-phase mode. The effects of the concentration of organic solvents (iso-propanol and ethanol), flow rate and column temperature on the enantiomer separation were studied systematically. The best separation was obtained with hexane-ethanol-diethyl amine (88 : 12 : 0.1, v/v/v) as mobile phase at 20 degrees C. The optimal flow rate was 0.6 mL/min. The detection wavelength was set at 270 nm. By using this method, the enantiomers of bisoprolol can be separated quickly and easily.
PMID: 19253562