char = D;code = 68 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = :;code = 58 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = i;code = 105 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = d;code = 100 char = y;code = 121 char = n;code = 110 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = s;code = 115 char = ?;code = 63 result sql = Disruption of the blood-testis barrier integrity by bisphenol A in vitro: is this a suitable model for studying blood-testis barrier dynamics?
Disruption of the blood-testis barrier integrity by bisphenol A in vitro: is this a suitable model for studying blood-testis barrier dynamics?char = B;code = 66 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = v;code = 118 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = z;code = 122 char = a;code = 97 char = r;code = 114 char = d;code = 100 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = e;code = 101 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = h;code = 104 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = H;code = 72 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = a;code = 97 char = r;code = 114 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = d;code = 100 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = u;code = 117 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = m;code = 109 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = u;code = 117 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = y;code = 121 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = l;code = 108 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = i;code = 105 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = v;code = 118 char = o;code = 111 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = b;code = 98 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = u;code = 117 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = a;code = 97 char = v;code = 118 char = a;code = 97 char = i;code = 105 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = y;code = 121 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = c;code = 99 char = i;code = 105 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = H;code = 72 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = d;code = 100 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = 2;code = 50 char = 0;code = 48 char = -;code = 45 char = d;code = 100 char = a;code = 97 char = y;code = 121 char = -;code = 45 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = l;code = 108 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = s;code = 115 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = w;code = 119 char = a;code = 97 char = r;code = 114 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = i;code = 105 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = i;code = 105 char = s;code = 115 char = h;code = 104 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = t;code = 116 char = ;code = 32 char = j;code = 106 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = k;code = 107 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = v;code = 118 char = o;code = 111 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = i;code = 105 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = t;code = 116 char = ;code = 32 char = j;code = 106 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = E;code = 69 char = R;code = 82 char = K;code = 75 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = c;code = 99 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = t;code = 116 char = ;code = 32 char = j;code = 106 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = s;code = 115 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = g;code = 103 char = a;code = 97 char = p;code = 112 char = ;code = 32 char = j;code = 106 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = .;code = 46 char = ;code = 32 char = S;code = 83 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = u;code = 117 char = a;code = 97 char = l;code = 108 char = -;code = 45 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = m;code = 109 char = u;code = 117 char = n;code = 110 char = o;code = 111 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = h;code = 104 char = n;code = 110 char = i;code = 105 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = c;code = 99 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = o;code = 111 char = c;code = 99 char = c;code = 99 char = l;code = 108 char = u;code = 117 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = x;code = 120 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 4;code = 52 char = 3;code = 51 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = N;code = 78 char = -;code = 45 char = c;code = 99 char = a;code = 97 char = d;code = 100 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = -;code = 45 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = m;code = 109 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = i;code = 105 char = b;code = 98 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = b;code = 98 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = i;code = 105 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = y;code = 121 char = n;code = 110 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = r;code = 114 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Bisphenol A, an estrogenic environmental toxicant, has been implicated to have hazardous effects on reproductive health in humans and rodents. However, there are conflicting reports in the literature regarding its effects on male reproductive function. In this study, it was shown that in adult rats treated with acute doses of bisphenol A, a small but statistically insignificant percentage of seminiferous tubules in the testes displayed signs of germ cell loss, consistent with some earlier reports. It also failed to disrupt the blood-testis barrier in vivo. This is possibly due to the low bioavailability of free bisphenol A in the systemic circulation. However, bisphenol A disrupted the blood-testis barrier when administered to immature 20-day-old rats, consistent with earlier reports concerning the higher susceptibility of immature rats towards bisphenol A. This observation was confirmed using primary Sertoli cells cultured in vitro with established tight junction-permeability barrier that mimicked the blood-testis barrier in vivo. The reversible disruption of Sertoli cell tight junction barrier by bisphenol A was associated with an activation of ERK, and a decline in the levels of selected proteins at the tight junction, basal ectoplasmic specialization, and gap junction at the blood-testis barrier. Studies by dual-labeled immunofluorescence analysis and biotinylation techniques also illustrated declining levels of occludin, connexin 43, and N-cadherin at the cell-cell interface following bisphenol A treatment. In summary, bisphenol A reversibly perturbs the integrity of the blood-testis barrier in Sertoli cells in vitro, which can also serve as a suitable model for studying the dynamics of the blood-testis barrier.
Publication: Int. J. Biochem. Cell Biol. 2009 Nov ;41(11)::2302-14. :doi: 10.1016/j.biocel.2009.05.016.
Publication Date: 2009 Nov
Study Author(s): Li, Michelle W M;Mruk, Dolores D;Lee, Will M;Cheng, C Yan;
Institution: Center for Biomedical Research, Population Council, 1230 York Avenue, New York, NY 10065, USA.
Shortcut link to this study:
Bisphenol A, an estrogenic environmental toxicant, has been implicated to have hazardous effects on reproductive health in humans and rodents. However, there are conflicting reports in the literature regarding its effects on male reproductive function. In this study, it was shown that in adult rats treated with acute doses of bisphenol A, a small but statistically insignificant percentage of seminiferous tubules in the testes displayed signs of germ cell loss, consistent with some earlier reports. It also failed to disrupt the blood-testis barrier in vivo. This is possibly due to the low bioavailability of free bisphenol A in the systemic circulation. However, bisphenol A disrupted the blood-testis barrier when administered to immature 20-day-old rats, consistent with earlier reports concerning the higher susceptibility of immature rats towards bisphenol A. This observation was confirmed using primary Sertoli cells cultured in vitro with established tight junction-permeability barrier that mimicked the blood-testis barrier in vivo. The reversible disruption of Sertoli cell tight junction barrier by bisphenol A was associated with an activation of ERK, and a decline in the levels of selected proteins at the tight junction, basal ectoplasmic specialization, and gap junction at the blood-testis barrier. Studies by dual-labeled immunofluorescence analysis and biotinylation techniques also illustrated declining levels of occludin, connexin 43, and N-cadherin at the cell-cell interface following bisphenol A treatment. In summary, bisphenol A reversibly perturbs the integrity of the blood-testis barrier in Sertoli cells in vitro, which can also serve as a suitable model for studying the dynamics of the blood-testis barrier.
PMID: 19497385