char = C;code = 67 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = -;code = 45 char = s;code = 115 char = u;code = 117 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = G;code = 71 char = M;code = 77 char = O;code = 79 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = O;code = 79 char = -;code = 45 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = -;code = 45 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = .;code = 46 result sql = Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation method.
Cell-surface modification of non-GMO without chemical treatment by novel GMO-coupled and -separated cocultivation method.char = W;code = 87 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = (;code = 40 char = G;code = 71 char = M;code = 77 char = );code = 41 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = F;code = 70 char = i;code = 105 char = r;code = 114 char = s;code = 115 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = w;code = 119 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = :;code = 58 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = t;code = 116 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = -;code = 45 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = -;code = 45 char = s;code = 115 char = e;code = 101 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = i;code = 105 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = G;code = 71 char = F;code = 70 char = P;code = 80 char = );code = 41 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = f;code = 102 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = E;code = 69 char = G;code = 71 char = F;code = 70 char = P;code = 80 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = a;code = 97 char = r;code = 114 char = g;code = 103 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = S;code = 83 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = y;code = 121 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = i;code = 105 char = s;code = 115 char = i;code = 105 char = a;code = 97 char = e;code = 101 char = ;code = 32 char = B;code = 66 char = Y;code = 89 char = 4;code = 52 char = 7;code = 55 char = 4;code = 52 char = 1;code = 49 char = D;code = 68 char = e;code = 101 char = l;code = 108 char = t;code = 116 char = a;code = 97 char = C;code = 67 char = Y;code = 89 char = C;code = 67 char = 8;code = 56 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = );code = 41 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = Y;code = 89 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = -;code = 45 char = s;code = 115 char = u;code = 117 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = -;code = 45 char = s;code = 115 char = u;code = 117 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = G;code = 71 char = M;code = 77 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = g;code = 103 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = G;code = 71 char = M;code = 77 char = O;code = 79 char = s;code = 115 char = );code = 41 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = G;code = 71 char = M;code = 77 char = O;code = 79 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = We developed a novel method to coat living non-genetically modified (GM) cells with functional recombinant proteins. First, we prepared GM yeast to secrete constructed proteins that have two domains: a functional domain and a binding domain that recognizes other cells. Second, we cocultivated GM and non-GM yeasts that share and coutilize the medium containing recombinant proteins produced by GM yeasts using a filter-membrane-separated cultivation reactor. We confirmed that GM yeast secreted enhanced green fluorescent protein (EGFP) fusion proteins to culture medium. After cocultivation, EGFP fusion proteins produced by GM yeast were targeted to non-GM yeast (Saccharomyces cerevisiae BY4741DeltaCYC8 strain) cell surface. Yeast cell-surface engineering is a useful method that enables the coating of GM yeast cell surface with recombinant proteins to produce highly stable and accumulated protein particles. The results of this study suggest that development of cell-surface engineering from GM organisms (GMOs) to living non-GMOs by our novel cocultivation method is possible.
Publication: Appl. Microbiol. Biotechnol. 2009 Feb ;82(2)::293-301. :doi: 10.1007/s00253-008-1787-5.
Publication Date: 2009 Feb
Study Author(s): Miura, Natsuko;Aoki, Wataru;Tokumoto, Naoki;Kuroda, Kouichi;Ueda, Mitsuyoshi;
Institution: Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto 606-8502, Japan.
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We developed a novel method to coat living non-genetically modified (GM) cells with functional recombinant proteins. First, we prepared GM yeast to secrete constructed proteins that have two domains: a functional domain and a binding domain that recognizes other cells. Second, we cocultivated GM and non-GM yeasts that share and coutilize the medium containing recombinant proteins produced by GM yeasts using a filter-membrane-separated cultivation reactor. We confirmed that GM yeast secreted enhanced green fluorescent protein (EGFP) fusion proteins to culture medium. After cocultivation, EGFP fusion proteins produced by GM yeast were targeted to non-GM yeast (Saccharomyces cerevisiae BY4741DeltaCYC8 strain) cell surface. Yeast cell-surface engineering is a useful method that enables the coating of GM yeast cell surface with recombinant proteins to produce highly stable and accumulated protein particles. The results of this study suggest that development of cell-surface engineering from GM organisms (GMOs) to living non-GMOs by our novel cocultivation method is possible.
PMID: 19039583