|
|
char = D;code = 68
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char = a;code = 97
char = l;code = 108
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char = f;code = 102
char = f;code = 102
char = e;code = 101
char = c;code = 99
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char = ;code = 32
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char = ;code = 32
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char = l;code = 108
char = l;code = 108
char = s;code = 115
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char = ;code = 32
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char = i;code = 105
char = m;code = 109
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = L;code = 76
char = N;code = 78
char = C;code = 67
char = a;code = 97
char = P;code = 80
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = g;code = 103
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char = o;code = 111
char = w;code = 119
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
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char = n;code = 110
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char = l;code = 108
char = o;code = 111
char = w;code = 119
char = ;code = 32
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char = e;code = 101
char = l;code = 108
char = e;code = 101
char = n;code = 110
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char = t;code = 116
char = e;code = 101
char = ;code = 32
char = c;code = 99
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char = c;code = 99
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char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = .;code = 46
result sql = Differential effects of selenium on benign and malignant prostate epithelial cells: stimulation of LNCaP cell growth by noncytotoxic, low selenite concentrations.
Differential effects of selenium on benign and malignant prostate epithelial cells: stimulation of LNCaP cell growth by noncytotoxic, low selenite concentrations.char = W;code = 87
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = h;code = 104
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char = h;code = 104
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char = i;code = 105
char = s;code = 115
char = ;code = 32
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char = h;code = 104
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = n;code = 110
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char = ;code = 32
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char = ;code = 32
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char = b;code = 98
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char = i;code = 105
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char = g;code = 103
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char = N;code = 78
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char = t;code = 116
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char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
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char = l;code = 108
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char = t;code = 116
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = 1;code = 49
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = 1;code = 49
char = 0;code = 48
char = ;code = 32
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char = 4;code = 52
char = ;code = 32
char = t;code = 116
char = o;code = 111
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char = 7;code = 55
char = 2;code = 50
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char = h;code = 104
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char = C;code = 67
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char = i;code = 105
char = f;code = 102
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char = r;code = 114
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char = ;code = 32
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char = a;code = 97
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char = ;code = 32
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char = e;code = 101
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char = a;code = 97
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char = s;code = 115
char = s;code = 115
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char = ;code = 32
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char = T;code = 84
char = T;code = 84
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result sql = We examined the hypothesis that nontoxic concentrations of selenium induce apoptosis and growth inhibition selectively in prostate cancer cells but not in benign prostate cells. Nontumorigenic BPH-1 prostate epithelial cells, androgen-sensitive LNCaP, and androgen-independent PC-3 prostate cancer cells were exposed to sodium selenite at 1 to 10 micromol/l for 24 to 72 h. Cell proliferation, viability, and apoptosis were assessed by MTT assay, trypan blue exclusion, flow cytometry, DNA laddering, and caspase activation. BPH-1 cells were more sensitive for cytotoxic selenium effects than malignant prostate cells, whereas LNCaP cells were more sensitive than PC-3 cells. At noncytotoxic selenium concentrations, there was no apoptosis in BPH-1 and PC-3 cells and no growth inhibition of LNCaP and BPH-1 cells. PC-3 cells were refractory to apoptosis induction but were growth inhibited at noncytotoxic concentrations. LNCaP cells were growth stimulated at 1 micromol/l and sensitive to apoptosis induction at higher noncytotoxic concentrations. Thus, noncytotoxic selenite concentrations did not induce growth inhibition or apoptosis selectively in prostate cancer cells. Growth stimulation of LNCaP cells by low concentrations suggests the possibility of adverse effects of selenium supplementation on hormone sensitive prostate cancer, whereas inhibition of PC-3 cell proliferation at noncytotoxic concentrations suggests potential benefit of selenium in advanced prostate cancer.
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char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = .;code = 46
result sql = Differential effects of selenium on benign and malignant prostate epithelial cells: stimulation of LNCaP cell growth by noncytotoxic, low selenite concentrations.
char = e;code = 101
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char = e;code = 101
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char = h;code = 104
char = y;code = 121
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char = s;code = 115
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = ;code = 32
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char = o;code = 111
char = n;code = 110
char = t;code = 116
char = o;code = 111
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char = i;code = 105
char = c;code = 99
char = ;code = 32
char = c;code = 99
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char = c;code = 99
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char = r;code = 114
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char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = s;code = 115
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = l;code = 108
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char = u;code = 117
char = m;code = 109
char = ;code = 32
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char = e;code = 101
char = ;code = 32
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char = o;code = 111
char = p;code = 112
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char = o;code = 111
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char = s;code = 115
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char = t;code = 116
char = h;code = 104
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = h;code = 104
char = i;code = 105
char = b;code = 98
char = i;code = 105
char = t;code = 116
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char = o;code = 111
char = n;code = 110
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char = s;code = 115
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char = e;code = 101
char = c;code = 99
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char = v;code = 118
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char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = b;code = 98
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char = t;code = 116
char = ;code = 32
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char = o;code = 111
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char = n;code = 110
char = ;code = 32
char = b;code = 98
char = e;code = 101
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char = g;code = 103
char = n;code = 110
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char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
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char = .;code = 46
char = ;code = 32
char = N;code = 78
char = o;code = 111
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char = t;code = 116
char = u;code = 117
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char = o;code = 111
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char = g;code = 103
char = e;code = 101
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char = c;code = 99
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char = B;code = 66
char = P;code = 80
char = H;code = 72
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char = 1;code = 49
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char = r;code = 114
char = o;code = 111
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char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = e;code = 101
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char = e;code = 101
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char = i;code = 105
char = a;code = 97
char = l;code = 108
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char = c;code = 99
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char = l;code = 108
char = l;code = 108
char = s;code = 115
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char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = r;code = 114
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char = g;code = 103
char = e;code = 101
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char = -;code = 45
char = s;code = 115
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char = s;code = 115
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char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
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char = L;code = 76
char = N;code = 78
char = C;code = 67
char = a;code = 97
char = P;code = 80
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char = P;code = 80
char = C;code = 67
char = -;code = 45
char = 3;code = 51
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char = p;code = 112
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char = e;code = 101
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char = c;code = 99
char = e;code = 101
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char = l;code = 108
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char = w;code = 119
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char = e;code = 101
char = ;code = 32
char = e;code = 101
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char = e;code = 101
char = d;code = 100
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char = t;code = 116
char = o;code = 111
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = m;code = 109
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char = s;code = 115
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char = l;code = 108
char = e;code = 101
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char = t;code = 116
char = e;code = 101
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char = a;code = 97
char = t;code = 116
char = ;code = 32
char = 1;code = 49
char = ;code = 32
char = t;code = 116
char = o;code = 111
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char = 1;code = 49
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char = m;code = 109
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char = m;code = 109
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char = /;code = 47
char = l;code = 108
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char = 2;code = 50
char = 4;code = 52
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char = t;code = 116
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char = ;code = 32
char = 7;code = 55
char = 2;code = 50
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char = C;code = 67
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char = e;code = 101
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char = s;code = 115
char = s;code = 115
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char = M;code = 77
char = T;code = 84
char = T;code = 84
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char = s;code = 115
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char = ;code = 32
char = D;code = 68
char = N;code = 78
char = A;code = 65
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char = l;code = 108
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char = d;code = 100
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char = L;code = 76
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char = o;code = 111
char = w;code = 119
char = t;code = 116
char = h;code = 104
char = ;code = 32
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char = c;code = 99
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char = t;code = 116
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char = s;code = 115
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char = b;code = 98
char = i;code = 105
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char = i;code = 105
char = t;code = 116
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char = ;code = 32
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char = e;code = 101
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char = f;code = 102
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char = e;code = 101
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char = c;code = 99
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result sql = We examined the hypothesis that nontoxic concentrations of selenium induce apoptosis and growth inhibition selectively in prostate cancer cells but not in benign prostate cells. Nontumorigenic BPH-1 prostate epithelial cells, androgen-sensitive LNCaP, and androgen-independent PC-3 prostate cancer cells were exposed to sodium selenite at 1 to 10 micromol/l for 24 to 72 h. Cell proliferation, viability, and apoptosis were assessed by MTT assay, trypan blue exclusion, flow cytometry, DNA laddering, and caspase activation. BPH-1 cells were more sensitive for cytotoxic selenium effects than malignant prostate cells, whereas LNCaP cells were more sensitive than PC-3 cells. At noncytotoxic selenium concentrations, there was no apoptosis in BPH-1 and PC-3 cells and no growth inhibition of LNCaP and BPH-1 cells. PC-3 cells were refractory to apoptosis induction but were growth inhibited at noncytotoxic concentrations. LNCaP cells were growth stimulated at 1 micromol/l and sensitive to apoptosis induction at higher noncytotoxic concentrations. Thus, noncytotoxic selenite concentrations did not induce growth inhibition or apoptosis selectively in prostate cancer cells. Growth stimulation of LNCaP cells by low concentrations suggests the possibility of adverse effects of selenium supplementation on hormone sensitive prostate cancer, whereas inhibition of PC-3 cell proliferation at noncytotoxic concentrations suggests potential benefit of selenium in advanced prostate cancer.
Publication: Nutr Cancer 2009 ;61(2)::251-64. :doi: 10.1080/01635580802398430.
Publication Date: 2009
Study Author(s): Kandaş, Nur Ozten;Randolph, Carla;Bosland, Maarten C;
Institution: Department of Pathology, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA.
Shortcut link to this study:
Publication Date: 2009
Study Author(s): Kandaş, Nur Ozten;Randolph, Carla;Bosland, Maarten C;
Institution: Department of Pathology, University of Illinois at Chicago, 840 South Wood Street, Chicago, IL 60612, USA.
Shortcut link to this study:
We examined the hypothesis that nontoxic concentrations of selenium induce apoptosis and growth inhibition selectively in prostate cancer cells but not in benign prostate cells. Nontumorigenic BPH-1 prostate epithelial cells, androgen-sensitive LNCaP, and androgen-independent PC-3 prostate cancer cells were exposed to sodium selenite at 1 to 10 micromol/l for 24 to 72 h. Cell proliferation, viability, and apoptosis were assessed by MTT assay, trypan blue exclusion, flow cytometry, DNA laddering, and caspase activation. BPH-1 cells were more sensitive for cytotoxic selenium effects than malignant prostate cells, whereas LNCaP cells were more sensitive than PC-3 cells. At noncytotoxic selenium concentrations, there was no apoptosis in BPH-1 and PC-3 cells and no growth inhibition of LNCaP and BPH-1 cells. PC-3 cells were refractory to apoptosis induction but were growth inhibited at noncytotoxic concentrations. LNCaP cells were growth stimulated at 1 micromol/l and sensitive to apoptosis induction at higher noncytotoxic concentrations. Thus, noncytotoxic selenite concentrations did not induce growth inhibition or apoptosis selectively in prostate cancer cells. Growth stimulation of LNCaP cells by low concentrations suggests the possibility of adverse effects of selenium supplementation on hormone sensitive prostate cancer, whereas inhibition of PC-3 cell proliferation at noncytotoxic concentrations suggests potential benefit of selenium in advanced prostate cancer.
PMID: 19235042
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