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A simple HPLC-fluorescence method for quantitation of curcuminoids and its application to turmeric products.char = A;code = 65 char = n;code = 110 char = ;code = 32 char = H;code = 72 char = P;code = 80 char = L;code = 76 char = C;code = 67 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = 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d;code = 100 char = e;code = 101 char = n;code = 110 char = z;code = 122 char = a;code = 97 char = ;code = 32 char = C;code = 67 char = D;code = 68 char = -;code = 45 char = C;code = 67 char = (;code = 40 char = 1;code = 49 char = 8;code = 56 char = );code = 41 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = l;code = 108 char = u;code = 117 char = m;code = 109 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = 2;code = 50 char = 5;code = 53 char = 0;code = 48 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 4;code = 52 char = .;code = 46 char = 6;code = 54 char = ;code = 32 char = m;code = 109 char = m;code = 109 char = ;;code = 59 char = ;code = 32 char = i;code = 105 char = .;code = 46 char = d;code = 100 char = .;code = 46 char = ,;code = 44 char = ;code = 32 char = 3;code = 51 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = );code = 41 char = 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= o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = C;code = 67 char = ,;code = 44 char = ;code = 32 char = D;code = 68 char = M;code = 77 char = C;code = 67 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = D;code = 68 char = M;code = 77 char = C;code = 67 char = ;code = 32 char = (;code = 40 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = -;code = 45 char = t;code = 116 char = o;code = 111 char = -;code = 45 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = ;code = 32 char = =;code = 61 char = ;code = 32 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 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108 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = 6;code = 54 char = .;code = 46 char = 1;code = 49 char = %;code = 37 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = c;code = 99 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = w;code = 119 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = d;code = 100 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = r;code = 114 char = u;code = 117 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = r;code = 114 char = u;code = 117 char = g;code = 103 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = An HPLC method using fluorescence detection for the quantitation of curcuminoids, such as curcumin (C), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric products is described. This method involves a simple ultrasonic extraction with methanol as a pretreatment of turmeric products. The separation of curcuminoids and 2,5-xylenol (internal standard) was achieved within 30 min on a Cadenza CD-C(18) column (250 x 4.6 mm; i.d., 3 microm) with a mixture of acetate buffer and CH(3)CN. The calibration curves of standard curcuminoids showed good linearities of more than 0.993 of the correlation coefficient. The instrumental detection limits for C, DMC and BDMC (signal-to-noise ratio = 3) were 1.5, 0.9 and 0.09 ng mL(-1), respectively. The relative standard deviations of intra-and inter-day assays by curcuminoids spiked to turmeric powder were less than 6.1%. The proposed method was successfully applied to determine curcuminoids in commercial turmeric products, such as turmeric powders, a tablet, a dressing, a beverage, tea, and crude drugs.
Publication: Anal Sci 2009 Mar ;25(3)::385-8.
Publication Date: 2009 Mar
Study Author(s): Zhang, Junwei;Jinnai, Sakie;Ikeda, Rie;Wada, Mitsuhiro;Hayashida, Shinjiro;Nakashima, Kenichiro;
Institution: Department of Clinical Pharmacy, Graduate School of Biomedical Sciences, Nagasaki University, Japan.
Shortcut link to this study:
An HPLC method using fluorescence detection for the quantitation of curcuminoids, such as curcumin (C), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) in turmeric products is described. This method involves a simple ultrasonic extraction with methanol as a pretreatment of turmeric products. The separation of curcuminoids and 2,5-xylenol (internal standard) was achieved within 30 min on a Cadenza CD-C(18) column (250 x 4.6 mm; i.d., 3 microm) with a mixture of acetate buffer and CH(3)CN. The calibration curves of standard curcuminoids showed good linearities of more than 0.993 of the correlation coefficient. The instrumental detection limits for C, DMC and BDMC (signal-to-noise ratio = 3) were 1.5, 0.9 and 0.09 ng mL(-1), respectively. The relative standard deviations of intra-and inter-day assays by curcuminoids spiked to turmeric powder were less than 6.1%. The proposed method was successfully applied to determine curcuminoids in commercial turmeric products, such as turmeric powders, a tablet, a dressing, a beverage, tea, and crude drugs.
PMID: 19276595