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char = t;code = 116
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char = m;code = 109
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char = t;code = 116
char = o;code = 111
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
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char = a;code = 97
char = m;code = 109
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char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = ;code = 32
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char = a;code = 97
char = l;code = 108
char = m;code = 109
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char = l;code = 108
char = l;code = 108
char = a;code = 97
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char = y;code = 121
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char = r;code = 114
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
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char = r;code = 114
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char = S;code = 83
char = a;code = 97
char = l;code = 108
char = m;code = 109
char = o;code = 111
char = n;code = 110
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = a;code = 97
char = ;code = 32
char = m;code = 109
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = v;code = 118
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = o;code = 111
char = .;code = 46
result sql = Microbial population profiles of the microflora associated with pre- and postharvest tomatoes contaminated with Salmonella typhimurium or Salmonella montevideo.
Microbial population profiles of the microflora associated with pre- and postharvest tomatoes contaminated with Salmonella typhimurium or Salmonella montevideo.
char = <;code = 60
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char = :;code = 58
char = ;code = 32
char = <;code = 60
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char = b;code = 98
char = >;code = 62
char = T;code = 84
char = o;code = 111
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char = d;code = 100
char = e;code = 101
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char = m;code = 109
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char = e;code = 101
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char = ;code = 32
char = p;code = 112
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char = e;code = 101
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char = l;code = 108
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char = ;code = 32
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char = u;code = 117
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char = D;code = 68
char = T;code = 84
char = 1;code = 49
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char = <;code = 60
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char = <;code = 60
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char = r;code = 114
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char = >;code = 62
char = <;code = 60
char = b;code = 98
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char = E;code = 69
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char = H;code = 72
char = O;code = 79
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char = A;code = 65
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char = ;code = 32
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char = S;code = 83
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char = L;code = 76
char = T;code = 84
char = S;code = 83
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char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
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char = a;code = 97
char = l;code = 108
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char = l;code = 108
char = a;code = 97
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char = ;code = 32
char = o;code = 111
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char = a;code = 97
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char = o;code = 111
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char = l;code = 108
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char = ;code = 32
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char = ;code = 32
char = c;code = 99
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char = o;code = 111
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char = ;code = 32
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char = c;code = 99
char = e;code = 101
char = ;code = 32
char = C;code = 67
char = a;code = 97
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char = b;code = 98
char = o;code = 111
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char = U;code = 85
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char = l;code = 108
char = i;code = 105
char = z;code = 122
char = a;code = 97
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char = ;code = 32
char = 1;code = 49
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char = r;code = 114
char = D;code = 68
char = N;code = 78
char = A;code = 65
char = -;code = 45
char = P;code = 80
char = C;code = 67
char = R;code = 82
char = ;code = 32
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char = o;code = 111
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char = l;code = 108
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = .;code = 46
char = ;code = 32
char = F;code = 70
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = ;code = 32
char = 1;code = 49
char = 6;code = 54
char = S;code = 83
char = ;code = 32
char = r;code = 114
char = D;code = 68
char = N;code = 78
char = A;code = 65
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
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char = i;code = 105
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char = i;code = 105
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char = m;code = 109
char = a;code = 97
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char = e;code = 101
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char = .;code = 46
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char = T;code = 84
char = h;code = 104
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char = C;code = 67
char = .;code = 46
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char = c;code = 99
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char = e;code = 101
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char = a;code = 97
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char = p;code = 112
char = e;code = 101
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char = s;code = 115
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
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char = e;code = 101
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = e;code = 101
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char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = p;code = 112
char = a;code = 97
char = t;code = 116
char = h;code = 104
char = o;code = 111
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = .;code = 46
result sql = AIMS : To determine the microflora profiles of pre- and postharvest tomatoes contaminated with Salmonella montevideo or S. typhimurium DT104.

METHODS AND RESULTS : Salmonella montevideo or S. typhimurium was inoculated onto the flowers of tomato plants with the microflora of the subsequent fruit examined using a combination of Source Carbon Utilization and 16S rDNA-PCR profiling. From 16S rDNA profiles it was evident that tomatoes derived from Salmonella inoculated plants harboured a different microbial population compared to nontreated controls. The same result was observed for tomatoes inoculated at postharvest and subsequently stored for 14 days at 15 degrees C. From sequencing analysis it was found that tomatoes derived from Salmonella inoculated plants but testing negative for the enteric pathogen, frequently harboured Enterobacter and Bacillus spp. In contrast, both bacterial types were not found associated with tomatoes testing positive for Salmonella.

CONCLUSIONS : Salmonella introduced onto tomatoes at pre- or postharvest alters the composition of the microbial community. The presence of Enterobacter and Bacillus spp negatively affects the persistence of Salmonella on preharvest tomatoes.

SIGNIFICANCE AND IMPACT OF THE STUDY : Salmonella appears to modify rather than become integrated into the microbial communities associated with tomatoes. Yet, the presence of antagonistic bacteria appears to reduce the persistence of the enteric pathogen.

Publication: J. Appl. Microbiol. 2009 Jul ;107(1)::329-38. :doi: 10.1111/j.1365-2672.2009.04211.x.
Publication Date: 2009 Jul
Study Author(s): Shi, X;Wu, Z;Namvar, A;Kostrzynska, M;Dunfield, K;Warriner, K;
Institution: Department of Food Science, University of Guelph, Guelph, ON, Canada.
Shortcut link to this study:
AIMS : To determine the microflora profiles of pre- and postharvest tomatoes contaminated with Salmonella montevideo or S. typhimurium DT104.

METHODS AND RESULTS : Salmonella montevideo or S. typhimurium was inoculated onto the flowers of tomato plants with the microflora of the subsequent fruit examined using a combination of Source Carbon Utilization and 16S rDNA-PCR profiling. From 16S rDNA profiles it was evident that tomatoes derived from Salmonella inoculated plants harboured a different microbial population compared to nontreated controls. The same result was observed for tomatoes inoculated at postharvest and subsequently stored for 14 days at 15 degrees C. From sequencing analysis it was found that tomatoes derived from Salmonella inoculated plants but testing negative for the enteric pathogen, frequently harboured Enterobacter and Bacillus spp. In contrast, both bacterial types were not found associated with tomatoes testing positive for Salmonella.

CONCLUSIONS : Salmonella introduced onto tomatoes at pre- or postharvest alters the composition of the microbial community. The presence of Enterobacter and Bacillus spp negatively affects the persistence of Salmonella on preharvest tomatoes.

SIGNIFICANCE AND IMPACT OF THE STUDY : Salmonella appears to modify rather than become integrated into the microbial communities associated with tomatoes. Yet, the presence of antagonistic bacteria appears to reduce the persistence of the enteric pathogen.
PMID: 19291236

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