char = M;code = 77 char = a;code = 97 char = g;code = 103 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = g;code = 103 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = o;code = 111 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = .;code = 46 result sql = Magnetic resonance imaging and biological properties of pancreatic islets labeled with iron oxide nanoparticles.
Magnetic resonance imaging and biological properties of pancreatic islets labeled with iron oxide nanoparticles.char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = o;code = 111 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = M;code = 77 char = R;code = 82 char = I;code = 73 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = R;code = 82 char = e;code = 101 char = s;code = 115 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = 2;code = 50 char = 5;code = 53 char = -;code = 45 char = 2;code = 50 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = ;code = 32 char = F;code = 70 char = e;code = 101 char = /;code = 47 char = m;code = 109 char = L;code = 76 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = c;code = 99 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = M;code = 77 char = R;code = 82 char = I;code = 73 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = l;code = 108 char = y;code = 121 char = -;code = 45 char = l;code = 108 char = -;code = 45 char = L;code = 76 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = P;code = 80 char = L;code = 76 char = L;code = 76 char = ,;code = 44 char = ;code = 32 char = 1;code = 49 char = .;code = 46 char = 5;code = 53 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = /;code = 47 char = m;code = 109 char = L;code = 76 char = );code = 41 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = 4;code = 52 char = 8;code = 56 char = ;code = 32 char = h;code = 104 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = r;code = 114 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = 9;code = 57 char = 0;code = 48 char = %;code = 37 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = ;code = 32 char = F;code = 70 char = e;code = 101 char = /;code = 47 char = m;code = 109 char = L;code = 76 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = i;code = 105 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = o;code = 111 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = -;code = 45 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = m;code = 109 char = u;code = 117 char = n;code = 110 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = R;code = 82 char = e;code = 101 char = s;code = 115 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = T;code = 84 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = l;code = 108 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = T;code = 84 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = -;code = 45 char = w;code = 119 char = e;code = 101 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = M;code = 77 char = R;code = 82 char = I;code = 73 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = 1;code = 49 char = .;code = 46 char = 5;code = 53 char = ;code = 32 char = T;code = 84 char = e;code = 101 char = s;code = 115 char = l;code = 108 char = a;code = 97 char = ;code = 32 char = M;code = 77 char = R;code = 82 char = ;code = 32 char = s;code = 115 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = .;code = 46 char = ;code = 32 char = L;code = 76 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = ;code = 32 char = F;code = 70 char = e;code = 101 char = /;code = 47 char = m;code = 109 char = L;code = 76 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = R;code = 82 char = e;code = 101 char = s;code = 115 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = P;code = 80 char = L;code = 76 char = L;code = 76 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = s;code = 115 char = u;code = 117 char = a;code = 97 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = T;code = 84 char = (;code = 40 char = 2;code = 50 char = );code = 41 char = -;code = 45 char = w;code = 119 char = e;code = 101 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = M;code = 77 char = R;code = 82 char = I;code = 73 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = T;code = 84 char = T;code = 84 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = N;code = 78 char = o;code = 111 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = w;code = 119 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = (;code = 40 char = 2;code = 50 char = .;code = 46 char = 9;code = 57 char = 1;code = 49 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 3;code = 51 char = 6;code = 54 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = 2;code = 50 char = .;code = 46 char = 8;code = 56 char = 3;code = 51 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 6;code = 54 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = x;code = 120 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = l;code = 108 char = u;code = 117 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = r;code = 114 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = R;code = 82 char = T;code = 84 char = -;code = 45 char = P;code = 80 char = C;code = 67 char = R;code = 82 char = ;code = 32 char = (;code = 40 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = P;code = 80 char = C;code = 67 char = R;code = 82 char = );code = 41 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = .;code = 46 char = 8;code = 56 char = 3;code = 51 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 2;code = 50 char = 5;code = 53 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = v;code = 118 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;;code = 59 char = ;code = 32 char = p;code = 112 char = ;code = 32 char = =;code = 61 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 0;code = 48 char = 5;code = 53 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = .;code = 46 char = 3;code = 51 char = 9;code = 57 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 1;code = 49 char = 8;code = 56 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = v;code = 118 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;;code = 59 char = ;code = 32 char = p;code = 112 char = ;code = 32 char = =;code = 61 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 8;code = 56 char = 5;code = 53 char = );code = 41 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = c;code = 99 char = a;code = 97 char = g;code = 103 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = .;code = 46 char = 2;code = 50 char = 8;code = 56 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 1;code = 49 char = 3;code = 51 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = v;code = 118 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;;code = 59 char = ;code = 32 char = p;code = 112 char = ;code = 32 char = =;code = 61 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 9;code = 57 char = );code = 41 char = .;code = 46 char = ;code = 32 char = E;code = 69 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = B;code = 66 char = E;code = 69 char = T;code = 84 char = A;code = 65 char = 2;code = 50 char = ;code = 32 char = (;code = 40 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = -;code = 45 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = E;code = 69 char = -;code = 45 char = b;code = 98 char = o;code = 111 char = x;code = 120 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = -;code = 45 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = .;code = 46 char = 6;code = 54 char = 7;code = 55 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 1;code = 49 char = 5;code = 53 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = v;code = 118 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = l;code = 108 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;;code = 59 char = ;code = 32 char = p;code = 112 char = ;code = 32 char = =;code = 61 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 2;code = 50 char = 9;code = 57 char = );code = 41 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = R;code = 82 char = e;code = 101 char = s;code = 115 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = s;code = 115 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = This study was undertaken to investigate the in vitro effect of islet labeling with iron oxide nanoparticles for MRI on islet viability, insulin secretion, and gene expression. Isolated rat islets were labeled with Resovist (25-200 microg Fe/mL, a clinically approved MRI contrast agent) in the presence or absence of poly-l-Lysine (PLL, 1.5 microg/mL) for 48 h. The iron content of labeled islets was found to increase in a dose-dependent manner. More than 90% of the islets were labeled with 100 microg Fe/mL. We confirmed the localizations of iron oxide nanoparticles within islet beta-cells by insulin immunostaining. As the concentration of Resovist increased, T(2) values as determined by T(2)-weighted MRI on a 1.5 Tesla MR scanner decreased. Labeling of 100 islets in a medium containing 100 microg Fe/mL of Resovist in the absence of PLL provided sufficient contrast for islet visualization on T(2)-weighted MRI. MTT assays showed that the viability of labeled islets was not different from that of unlabeled islets. No statistical difference was observed between labeled (2.91 +/- 0.36) and unlabeled islets (2.83 +/- 0.61) in terms of the ability to secrete insulin, as determined by the glucose stimulation index. We also evaluated the effect of iron oxide incorporation on the gene expressions in islet cells using RT-PCR (reverse transcriptase PCR). Insulin expression in labeled islets was significantly elevated (1.83 +/- 0.25 fold vs. unlabeled; p = 0.005), but not the expression of somatostatin (1.39 +/- 0.18 fold vs. unlabeled; p = 0.085) or glucagons (1.28 +/- 0.13 fold vs. unlabeled; p = 0.09). Expression of an important transcription factor for insulin gene transcription, BETA2 (beta-cell E-box trans-activator), was increased in labeled islets (1.67 +/- 0.15 fold vs. unlabeled; p = 0.029). The findings of this study indicate that Resovist provides a satisfactory means to image islets and has no deleterious effect on islet function or gene expression.
Publication: NMR Biomed 2009 Oct ;22(8)::852-6. :doi: 10.1002/nbm.1398.
Publication Date: 2009 Oct
Study Author(s): Kim, Hoe Suk;Choi, YoonSeok;Song, In Chan;Moon, Woo Kyung;
Institution: Diagnostic Radiology, Seoul National University Hospital, Institute of Radiation Medicine, Medical Research Center, Seoul National University, 28, Yeongeon-dong, Jongno-gu, Seoul 110-744, Republic of Korea.
Shortcut link to this study:
This study was undertaken to investigate the in vitro effect of islet labeling with iron oxide nanoparticles for MRI on islet viability, insulin secretion, and gene expression. Isolated rat islets were labeled with Resovist (25-200 microg Fe/mL, a clinically approved MRI contrast agent) in the presence or absence of poly-l-Lysine (PLL, 1.5 microg/mL) for 48 h. The iron content of labeled islets was found to increase in a dose-dependent manner. More than 90% of the islets were labeled with 100 microg Fe/mL. We confirmed the localizations of iron oxide nanoparticles within islet beta-cells by insulin immunostaining. As the concentration of Resovist increased, T(2) values as determined by T(2)-weighted MRI on a 1.5 Tesla MR scanner decreased. Labeling of 100 islets in a medium containing 100 microg Fe/mL of Resovist in the absence of PLL provided sufficient contrast for islet visualization on T(2)-weighted MRI. MTT assays showed that the viability of labeled islets was not different from that of unlabeled islets. No statistical difference was observed between labeled (2.91 +/- 0.36) and unlabeled islets (2.83 +/- 0.61) in terms of the ability to secrete insulin, as determined by the glucose stimulation index. We also evaluated the effect of iron oxide incorporation on the gene expressions in islet cells using RT-PCR (reverse transcriptase PCR). Insulin expression in labeled islets was significantly elevated (1.83 +/- 0.25 fold vs. unlabeled; p = 0.005), but not the expression of somatostatin (1.39 +/- 0.18 fold vs. unlabeled; p = 0.085) or glucagons (1.28 +/- 0.13 fold vs. unlabeled; p = 0.09). Expression of an important transcription factor for insulin gene transcription, BETA2 (beta-cell E-box trans-activator), was increased in labeled islets (1.67 +/- 0.15 fold vs. unlabeled; p = 0.029). The findings of this study indicate that Resovist provides a satisfactory means to image islets and has no deleterious effect on islet function or gene expression.
PMID: 19489018