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char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = In this study, the interaction of the endocrine disruptor bisphenol A (BPA) and human serum albumin (HSA) was investigated by molecular modelling, fluorescence, ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR) and circular dichroism spectroscopy (CD). The association constants between BPA and HSA were determined using the Scatchard equation. The thermodynamic parameters of the binding reaction (DeltaG0, DeltaH0 and DeltaS0) were measured, and they indicated the presence of hydrophobic forces in the BPA-HSA interaction, which agreed well with the results from molecular modelling. The alterations of protein secondary structure in the presence of BPA were confirmed by UV-vis, FT-IR and CD spectroscopy. Lastly, the average binding distance, r, between BPA and HSA was evaluated and found to be 1.82 nm according to Förster's theory of non-radiation energy transfer.
Publication: Chemosphere 2010 Aug ;80(9)::1075-80. :doi: 10.1016/j.chemosphere.2010.04.076.
Publication Date: 2010 Aug
Study Author(s): Xie, Xiaoyun;Wang, Xiaoru;Xu, Xiangmei;Sun, Huijun;Chen, Xingguo;
Institution: National Key Laboratory of Organic Chemistry, Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
Shortcut link to this study:
In this study, the interaction of the endocrine disruptor bisphenol A (BPA) and human serum albumin (HSA) was investigated by molecular modelling, fluorescence, ultraviolet-visible spectroscopy (UV-vis), Fourier transform infrared spectroscopy (FT-IR) and circular dichroism spectroscopy (CD). The association constants between BPA and HSA were determined using the Scatchard equation. The thermodynamic parameters of the binding reaction (DeltaG0, DeltaH0 and DeltaS0) were measured, and they indicated the presence of hydrophobic forces in the BPA-HSA interaction, which agreed well with the results from molecular modelling. The alterations of protein secondary structure in the presence of BPA were confirmed by UV-vis, FT-IR and CD spectroscopy. Lastly, the average binding distance, r, between BPA and HSA was evaluated and found to be 1.82 nm according to Förster's theory of non-radiation energy transfer.
PMID: 20510433