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result sql = [Study on the iodine 125 uptake of H460 lung cancer cell line by co-transfection with the human sodium/iodide symporter and the human thyroperoxidase].
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char = g;code = 103
char = e;code = 101
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char = e;code = 101
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char = e;code = 101
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char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
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char = (;code = 40
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
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char = ;code = 32
char = s;code = 115
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char = l;code = 108
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
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char = G;code = 71
char = 4;code = 52
char = 1;code = 49
char = 8;code = 56
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char = a;code = 97
char = n;code = 110
char = t;code = 116
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char = b;code = 98
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char = o;code = 111
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = s;code = 115
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = .;code = 46
char = ;code = 32
char = U;code = 85
char = s;code = 115
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = ,;code = 44
char = ;code = 32
char = h;code = 104
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char = P;code = 80
char = O;code = 79
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char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = .;code = 46
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = o;code = 111
char = u;code = 117
char = t;code = 116
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = p;code = 112
char = p;code = 112
char = l;code = 108
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = ;code = 32
char = (;code = 40
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = );code = 41
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = v;code = 118
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = i;code = 105
char = g;code = 103
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = ;code = 32
char = u;code = 117
char = p;code = 112
char = t;code = 116
char = a;code = 97
char = k;code = 107
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = a;code = 97
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = b;code = 98
char = o;code = 111
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = R;code = 82
char = E;code = 69
char = S;code = 83
char = U;code = 85
char = L;code = 76
char = T;code = 84
char = S;code = 83
char = ;code = 32
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = W;code = 87
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = u;code = 117
char = c;code = 99
char = c;code = 99
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = f;code = 102
char = u;code = 117
char = l;code = 108
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = -;code = 45
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = T;code = 84
char = P;code = 80
char = O;code = 79
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char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = o;code = 111
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char = h;code = 104
char = u;code = 117
char = m;code = 109
char = a;code = 97
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char = l;code = 108
char = u;code = 117
char = n;code = 110
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char = c;code = 99
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char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
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char = ,;code = 44
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = o;code = 111
char = b;code = 98
char = t;code = 116
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = T;code = 84
char = P;code = 80
char = O;code = 79
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char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
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char = l;code = 108
char = u;code = 117
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = c;code = 99
char = a;code = 97
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char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
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char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = );code = 41
char = .;code = 46
char = ;code = 32
char = I;code = 73
char = n;code = 110
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = u;code = 117
char = p;code = 112
char = t;code = 116
char = a;code = 97
char = k;code = 107
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 5;code = 53
char = 9;code = 57
char = ;code = 32
char = 6;code = 54
char = 3;code = 51
char = 7;code = 55
char = .;code = 46
char = 6;code = 54
char = 7;code = 55
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 1;code = 49
char = ;code = 32
char = 2;code = 50
char = 8;code = 56
char = 1;code = 49
char = .;code = 46
char = 1;code = 49
char = 3;code = 51
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = (;code = 40
char = 4;code = 52
char = 8;code = 56
char = ;code = 32
char = 6;code = 54
char = 2;code = 50
char = 2;code = 50
char = .;code = 46
char = 1;code = 49
char = 7;code = 55
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 2;code = 50
char = ;code = 32
char = 2;code = 50
char = 4;code = 52
char = 2;code = 50
char = .;code = 46
char = 2;code = 50
char = 8;code = 56
char = );code = 41
char = ;code = 32
char = a;code = 97
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char = d;code = 100
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char = (;code = 40
char = 1;code = 49
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char = 4;code = 52
char = 4;code = 52
char = 0;code = 48
char = .;code = 46
char = 1;code = 49
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char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 3;code = 51
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char = 2;code = 50
char = .;code = 46
char = 8;code = 56
char = 6;code = 54
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char = ;code = 32
char = c;code = 99
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char = u;code = 117
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char = t;code = 116
char = s;code = 115
char = ;code = 32
char = x;code = 120
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = (;code = 40
char = -;code = 45
char = 1;code = 49
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char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = u;code = 117
char = p;code = 112
char = t;code = 116
char = a;code = 97
char = k;code = 107
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char = a;code = 97
char = b;code = 98
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char = l;code = 108
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char = t;code = 116
char = y;code = 121
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char = o;code = 111
char = f;code = 102
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char = I;code = 73
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char = w;code = 119
char = a;code = 97
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char = f;code = 102
char = o;code = 111
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char = h;code = 104
char = e;code = 101
char = r;code = 114
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char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
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char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
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char = a;code = 97
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char = b;code = 98
char = l;code = 108
char = a;code = 97
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char = k;code = 107
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char = c;code = 99
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char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
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char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = ;code = 32
char = <;code = 60
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char = 0;code = 48
char = .;code = 46
char = 0;code = 48
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char = ,;code = 44
char = ;code = 32
char = a;code = 97
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char = 4;code = 52
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char = f;code = 102
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char = h;code = 104
char = i;code = 105
char = g;code = 103
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
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char = b;code = 98
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char = a;code = 97
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char = k;code = 107
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char = t;code = 116
char = r;code = 114
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char = l;code = 108
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = ;code = 32
char = <;code = 60
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char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 1;code = 49
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char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = 1;code = 49
char = .;code = 46
char = 2;code = 50
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char = f;code = 102
char = o;code = 111
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char = d;code = 100
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char = h;code = 104
char = i;code = 105
char = g;code = 103
char = h;code = 104
char = e;code = 101
char = r;code = 114
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = ;code = 32
char = <;code = 60
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 0;code = 48
char = 1;code = 49
char = );code = 41
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
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result sql = BACKGROUND AND OBJECTIVE : Lung cancer harms people's health or even lives severely. Especially, the therapy of non-small cell lung cancer (NSCLC) has not been obviously improved for many years. The aim of this study is to transfer the human sodium/iodide symporter (hNIS) and the human thyroperoxidase (hTPO) genes into H460 lung cancer cell line, and to study the uptake ability of iodide after co-transfected hTPO and hNIS gene in cell lines.
METHODS : Through cloning, recombination, packaging and amplifying, the recombinant adenosine virus (AdTPO) was constructed. Then the protein expression of AdTPO was tested by Western blot. After transfected hNIS gene into human lung cancer cell line H460 through liposome, stably expressing hNIS gene cell lines (hNIS-H460) selected by G418 antibiotics was determined as hNIS-H460 group. Using AdTPO, hTPO gene was transducted into hNIS-H460, as AdTPO-hNIS-H460 group. H460 cell without hNIS gene was applied as control group (H460). Then, we investigated the 125I uptake assay of the above cells.
RESULTS : We were successful in co-transfecting hNIS and hTPO gene into human lung cell lines H460, and were obtained hNIS and hTPO gene lung cancer cell lines (hNIS-H460 and AdTPO-hNIS-H460). In AdTPO-hNIS-H460, hNIS-H460 and H460, the uptake ability of 125I was (59 637.67 +/- 1 281.13), (48 622.17 +/- 2 242.28) and (1 440.17 +/- 372.86) counts x min(-1). The uptake ability of 125I was 41 fold higher in AdTPO-hNIS-H460 than in blank control H460 (P < 0.01), and 34 fold higher in hNIS-460 than in blank control H460 (P < 0.01), and 1.2 fold higher in AdTPO-hNIS-H460 than in hNIS-H460 (P < 0.01).
CONCLUSION : The uptake ability of 125I could increase by co-transfected hNIS and hTPO genes into human lung cancer cell lines H460.
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result sql = [Study on the iodine 125 uptake of H460 lung cancer cell line by co-transfection with the human sodium/iodide symporter and the human thyroperoxidase].
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char = s;code = 115
char = ;code = 32
char = (;code = 40
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = );code = 41
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = t;code = 116
char = r;code = 114
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = n;code = 110
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = W;code = 87
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = n;code = 110
char = ;code = 32
char = b;code = 98
char = l;code = 108
char = o;code = 111
char = t;code = 116
char = .;code = 46
char = ;code = 32
char = A;code = 65
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = h;code = 104
char = u;code = 117
char = m;code = 109
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = l;code = 108
char = u;code = 117
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = g;code = 103
char = h;code = 104
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = p;code = 112
char = o;code = 111
char = s;code = 115
char = o;code = 111
char = m;code = 109
char = e;code = 101
char = ,;code = 44
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = y;code = 121
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = );code = 41
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = l;code = 108
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = G;code = 71
char = 4;code = 52
char = 1;code = 49
char = 8;code = 56
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = b;code = 98
char = i;code = 105
char = o;code = 111
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = .;code = 46
char = ;code = 32
char = U;code = 85
char = s;code = 115
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = ,;code = 44
char = ;code = 32
char = h;code = 104
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = .;code = 46
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = o;code = 111
char = u;code = 117
char = t;code = 116
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = p;code = 112
char = p;code = 112
char = l;code = 108
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = ;code = 32
char = (;code = 40
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = );code = 41
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = v;code = 118
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = i;code = 105
char = g;code = 103
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = ;code = 32
char = u;code = 117
char = p;code = 112
char = t;code = 116
char = a;code = 97
char = k;code = 107
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = a;code = 97
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = b;code = 98
char = o;code = 111
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = R;code = 82
char = E;code = 69
char = S;code = 83
char = U;code = 85
char = L;code = 76
char = T;code = 84
char = S;code = 83
char = ;code = 32
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = W;code = 87
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = u;code = 117
char = c;code = 99
char = c;code = 99
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = f;code = 102
char = u;code = 117
char = l;code = 108
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = -;code = 45
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = h;code = 104
char = u;code = 117
char = m;code = 109
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = l;code = 108
char = u;code = 117
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = b;code = 98
char = t;code = 116
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = l;code = 108
char = u;code = 117
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = );code = 41
char = .;code = 46
char = ;code = 32
char = I;code = 73
char = n;code = 110
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = T;code = 84
char = P;code = 80
char = O;code = 79
char = -;code = 45
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = h;code = 104
char = N;code = 78
char = I;code = 73
char = S;code = 83
char = -;code = 45
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = H;code = 72
char = 4;code = 52
char = 6;code = 54
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = u;code = 117
char = p;code = 112
char = t;code = 116
char = a;code = 97
char = k;code = 107
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 5;code = 53
char = 9;code = 57
char = ;code = 32
char = 6;code = 54
char = 3;code = 51
char = 7;code = 55
char = .;code = 46
char = 6;code = 54
char = 7;code = 55
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 1;code = 49
char = ;code = 32
char = 2;code = 50
char = 8;code = 56
char = 1;code = 49
char = .;code = 46
char = 1;code = 49
char = 3;code = 51
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = (;code = 40
char = 4;code = 52
char = 8;code = 56
char = ;code = 32
char = 6;code = 54
char = 2;code = 50
char = 2;code = 50
char = .;code = 46
char = 1;code = 49
char = 7;code = 55
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 2;code = 50
char = ;code = 32
char = 2;code = 50
char = 4;code = 52
char = 2;code = 50
char = .;code = 46
char = 2;code = 50
char = 8;code = 56
char = );code = 41
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = ;code = 32
char = 4;code = 52
char = 4;code = 52
char = 0;code = 48
char = .;code = 46
char = 1;code = 49
char = 7;code = 55
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 3;code = 51
char = 7;code = 55
char = 2;code = 50
char = .;code = 46
char = 8;code = 56
char = 6;code = 54
char = );code = 41
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = u;code = 117
char = n;code = 110
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = x;code = 120
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = (;code = 40
char = -;code = 45
char = 1;code = 49
char = );code = 41
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = u;code = 117
char = p;code = 112
char = t;code = 116
char = a;code = 97
char = k;code = 107
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = 5;code = 53
char = I;code = 73
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = 4;code = 52
char = 1;code = 49
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = l;code = 108
char = d;code = 100
char = ;code = 32
char = h;code = 104
char = i;code = 105
char = g;code = 103
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = i;code = 105
char = n;code = 110
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char = l;code = 108
char = a;code = 97
char = n;code = 110
char = k;code = 107
char = ;code = 32
char = c;code = 99
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char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
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result sql = BACKGROUND AND OBJECTIVE : Lung cancer harms people's health or even lives severely. Especially, the therapy of non-small cell lung cancer (NSCLC) has not been obviously improved for many years. The aim of this study is to transfer the human sodium/iodide symporter (hNIS) and the human thyroperoxidase (hTPO) genes into H460 lung cancer cell line, and to study the uptake ability of iodide after co-transfected hTPO and hNIS gene in cell lines.
METHODS : Through cloning, recombination, packaging and amplifying, the recombinant adenosine virus (AdTPO) was constructed. Then the protein expression of AdTPO was tested by Western blot. After transfected hNIS gene into human lung cancer cell line H460 through liposome, stably expressing hNIS gene cell lines (hNIS-H460) selected by G418 antibiotics was determined as hNIS-H460 group. Using AdTPO, hTPO gene was transducted into hNIS-H460, as AdTPO-hNIS-H460 group. H460 cell without hNIS gene was applied as control group (H460). Then, we investigated the 125I uptake assay of the above cells.
RESULTS : We were successful in co-transfecting hNIS and hTPO gene into human lung cell lines H460, and were obtained hNIS and hTPO gene lung cancer cell lines (hNIS-H460 and AdTPO-hNIS-H460). In AdTPO-hNIS-H460, hNIS-H460 and H460, the uptake ability of 125I was (59 637.67 +/- 1 281.13), (48 622.17 +/- 2 242.28) and (1 440.17 +/- 372.86) counts x min(-1). The uptake ability of 125I was 41 fold higher in AdTPO-hNIS-H460 than in blank control H460 (P < 0.01), and 34 fold higher in hNIS-460 than in blank control H460 (P < 0.01), and 1.2 fold higher in AdTPO-hNIS-H460 than in hNIS-H460 (P < 0.01).
CONCLUSION : The uptake ability of 125I could increase by co-transfected hNIS and hTPO genes into human lung cancer cell lines H460.
Publication: Zhongguo Fei Ai Za Zhi 2010 Jun ;13(6)::575-9. :doi: 10.3779/j.issn.1009-3419.2010.06.002.
Publication Date: 2010 Jun
Study Author(s): Li, Wei;Tan, Jian;Long, Lei;
Institution: Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Shortcut link to this study:
Publication Date: 2010 Jun
Study Author(s): Li, Wei;Tan, Jian;Long, Lei;
Institution: Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China.
Shortcut link to this study:
BACKGROUND AND OBJECTIVE : Lung cancer harms people's health or even lives severely. Especially, the therapy of non-small cell lung cancer (NSCLC) has not been obviously improved for many years. The aim of this study is to transfer the human sodium/iodide symporter (hNIS) and the human thyroperoxidase (hTPO) genes into H460 lung cancer cell line, and to study the uptake ability of iodide after co-transfected hTPO and hNIS gene in cell lines.
METHODS : Through cloning, recombination, packaging and amplifying, the recombinant adenosine virus (AdTPO) was constructed. Then the protein expression of AdTPO was tested by Western blot. After transfected hNIS gene into human lung cancer cell line H460 through liposome, stably expressing hNIS gene cell lines (hNIS-H460) selected by G418 antibiotics was determined as hNIS-H460 group. Using AdTPO, hTPO gene was transducted into hNIS-H460, as AdTPO-hNIS-H460 group. H460 cell without hNIS gene was applied as control group (H460). Then, we investigated the 125I uptake assay of the above cells.
RESULTS : We were successful in co-transfecting hNIS and hTPO gene into human lung cell lines H460, and were obtained hNIS and hTPO gene lung cancer cell lines (hNIS-H460 and AdTPO-hNIS-H460). In AdTPO-hNIS-H460, hNIS-H460 and H460, the uptake ability of 125I was (59 637.67 +/- 1 281.13), (48 622.17 +/- 2 242.28) and (1 440.17 +/- 372.86) counts x min(-1). The uptake ability of 125I was 41 fold higher in AdTPO-hNIS-H460 than in blank control H460 (P < 0.01), and 34 fold higher in hNIS-460 than in blank control H460 (P < 0.01), and 1.2 fold higher in AdTPO-hNIS-H460 than in hNIS-H460 (P < 0.01).
CONCLUSION : The uptake ability of 125I could increase by co-transfected hNIS and hTPO genes into human lung cancer cell lines H460.
METHODS : Through cloning, recombination, packaging and amplifying, the recombinant adenosine virus (AdTPO) was constructed. Then the protein expression of AdTPO was tested by Western blot. After transfected hNIS gene into human lung cancer cell line H460 through liposome, stably expressing hNIS gene cell lines (hNIS-H460) selected by G418 antibiotics was determined as hNIS-H460 group. Using AdTPO, hTPO gene was transducted into hNIS-H460, as AdTPO-hNIS-H460 group. H460 cell without hNIS gene was applied as control group (H460). Then, we investigated the 125I uptake assay of the above cells.
RESULTS : We were successful in co-transfecting hNIS and hTPO gene into human lung cell lines H460, and were obtained hNIS and hTPO gene lung cancer cell lines (hNIS-H460 and AdTPO-hNIS-H460). In AdTPO-hNIS-H460, hNIS-H460 and H460, the uptake ability of 125I was (59 637.67 +/- 1 281.13), (48 622.17 +/- 2 242.28) and (1 440.17 +/- 372.86) counts x min(-1). The uptake ability of 125I was 41 fold higher in AdTPO-hNIS-H460 than in blank control H460 (P < 0.01), and 34 fold higher in hNIS-460 than in blank control H460 (P < 0.01), and 1.2 fold higher in AdTPO-hNIS-H460 than in hNIS-H460 (P < 0.01).
CONCLUSION : The uptake ability of 125I could increase by co-transfected hNIS and hTPO genes into human lung cancer cell lines H460.
PMID: 20681442
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