char = O;code = 79
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char = s;code = 115
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char = p;code = 112
char = o;code = 111
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char = s;code = 115
char = .;code = 46
result sql = Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.
Optimization of biodiesel production catalyzed by fungus cells immobilized in fibrous supports.
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char = a;code = 97
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char = ;code = 32
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char = s;code = 115
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char = f;code = 102
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char = ;code = 32
char = s;code = 115
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char = a;code = 97
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char = l;code = 108
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char = f;code = 102
char = o;code = 111
char = r;code = 114
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char = s;code = 115
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char = m;code = 109
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char = l;code = 108
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char = s;code = 115
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char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
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char = t;code = 116
char = h;code = 104
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = m;code = 109
char = m;code = 109
char = o;code = 111
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = z;code = 122
char = a;code = 97
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char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = R;code = 82
char = h;code = 104
char = i;code = 105
char = z;code = 122
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char = u;code = 117
char = s;code = 115
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char = a;code = 97
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char = f;code = 102
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char = c;code = 99
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char = l;code = 108
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char = ;code = 32
char = c;code = 99
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char = o;code = 111
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char = p;code = 112
char = a;code = 97
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char = f;code = 102
char = ;code = 32
char = s;code = 115
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char = y;code = 121
char = b;code = 98
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char = a;code = 97
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = i;code = 105
char = l;code = 108
char = .;code = 46
char = ;code = 32
char = R;code = 82
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char = s;code = 115
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char = o;code = 111
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char = s;code = 115
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char = a;code = 97
char = c;code = 99
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char = m;code = 109
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char = o;code = 111
char = l;code = 108
char = o;code = 111
char = g;code = 103
char = y;code = 121
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char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
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char = e;code = 101
char = l;code = 108
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char = f;code = 102
char = a;code = 97
char = c;code = 99
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char = o;code = 111
char = r;code = 114
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char = c;code = 99
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char = a;code = 97
char = l;code = 108
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char = c;code = 99
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char = o;code = 111
char = s;code = 115
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char = a;code = 97
char = t;code = 116
char = a;code = 97
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char = a;code = 97
char = s;code = 115
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char = r;code = 114
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char = e;code = 101
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char = t;code = 116
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char = b;code = 98
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char = ;code = 32
char = a;code = 97
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char = o;code = 111
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char = e;code = 101
char = r;code = 114
char = f;code = 102
char = u;code = 117
char = l;code = 108
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = p;code = 112
char = t;code = 116
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char = i;code = 105
char = z;code = 122
char = a;code = 97
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char = i;code = 105
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char = f;code = 102
char = ;code = 32
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char = a;code = 97
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char = o;code = 111
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char = y;code = 121
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char = s;code = 115
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char = c;code = 99
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char = i;code = 105
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char = s;code = 115
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char = a;code = 97
char = l;code = 108
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char = e;code = 101
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char = i;code = 105
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char = m;code = 109
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char = .;code = 46
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char = c;code = 99
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char = l;code = 108
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char = b;code = 98
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char = A;code = 65
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char = i;code = 105
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char = i;code = 105
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char = o;code = 111
char = f;code = 102
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result sql = A circulating packed-bed bioreactor system using fibrous nonwoven fabric as the immobilization matrix was suitable for simultaneous cell growth and immobilization of Rhizopus oryzae fungus cells, which could be used for lipase-mediated production of biodiesel by methanolysis of soybean oil. Response surface methodology and 5-level-5-factor central composite rotatable design was proved to be a powerful tool for the optimization of methanolysis conditions catalyzed by immobilized R. oryzae whole cell biocatalyst. A quadratic polynomial regression model was used to analyze the relationship between the yield and the significant reaction parameters. The analysis confirmed that water content, molar ratio of methanol to oil, cell weight, and reaction time were the significant factors affecting the yield at a 95% confidence level (p < 0.05). Under the optimum condition at 10.97% (w/w) water content, 0.64 molar ratio of methanol to oil, 2.25% (w/w) cell weight, and 23.3 h reaction time, the predicted value of yield was 72.6%. Validation experiments with yields of 70.77 +/- 2.46% verified the availability and the accuracy of the model.

Publication: Appl. Biochem. Biotechnol. 2010 May ;161(1-8)::181-94. :doi: 10.1007/s12010-009-8776-8.
Publication Date: 2010 May
Study Author(s): Chen, Jyh-Ping;Lin, Gen-Hsu;
Institution: Department of Chemical and Materials Engineering, Chang Gung University, Kwei-San, Taoyuan 333, Taiwan, Republic of China. jpchen@mail.cgu.edu.tw
Shortcut link to this study:
A circulating packed-bed bioreactor system using fibrous nonwoven fabric as the immobilization matrix was suitable for simultaneous cell growth and immobilization of Rhizopus oryzae fungus cells, which could be used for lipase-mediated production of biodiesel by methanolysis of soybean oil. Response surface methodology and 5-level-5-factor central composite rotatable design was proved to be a powerful tool for the optimization of methanolysis conditions catalyzed by immobilized R. oryzae whole cell biocatalyst. A quadratic polynomial regression model was used to analyze the relationship between the yield and the significant reaction parameters. The analysis confirmed that water content, molar ratio of methanol to oil, cell weight, and reaction time were the significant factors affecting the yield at a 95% confidence level (p < 0.05). Under the optimum condition at 10.97% (w/w) water content, 0.64 molar ratio of methanol to oil, 2.25% (w/w) cell weight, and 23.3 h reaction time, the predicted value of yield was 72.6%. Validation experiments with yields of 70.77 +/- 2.46% verified the availability and the accuracy of the model.
PMID: 19777379

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