char = D;code = 68 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = l;code = 108 char = y;code = 121 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = k;code = 107 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = (;code = 40 char = C;code = 67 char = u;code = 117 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = a;code = 97 char = ;code = 32 char = L;code = 76 char = .;code = 46 char = );code = 41 char = .;code = 46 result sql = Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).
Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).char = E;code = 69 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = S;code = 83 char = T;code = 84 char = s;code = 115 char = );code = 41 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = (;code = 40 char = C;code = 67 char = u;code = 117 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = a;code = 97 char = ;code = 32 char = L;code = 76 char = .;code = 46 char = );code = 41 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = C;code = 67 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = I;code = 73 char = ;code = 32 char = (;code = 40 char = h;code = 104 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = );code = 41 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = S;code = 83 char = S;code = 83 char = R;code = 82 char = s;code = 115 char = );code = 41 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = 2;code = 50 char = 3;code = 51 char = 1;code = 49 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = 1;code = 49 char = 2;code = 50 char = ,;code = 44 char = 5;code = 53 char = 9;code = 57 char = 3;code = 51 char = ;code = 32 char = E;code = 69 char = S;code = 83 char = T;code = 84 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = f;code = 102 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = y;code = 121 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = C;code = 67 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = I;code = 73 char = ;code = 32 char = S;code = 83 char = S;code = 83 char = R;code = 82 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = S;code = 83 char = R;code = 82 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = 1;code = 49 char = 7;code = 55 char = .;code = 46 char = 9;code = 57 char = 6;code = 54 char = ;code = 32 char = k;code = 107 char = b;code = 98 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = E;code = 69 char = S;code = 83 char = T;code = 84 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = E;code = 69 char = S;code = 83 char = T;code = 84 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = u;code = 117 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = u;code = 117 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = 1;code = 49 char = 7;code = 55 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = l;code = 108 char = y;code = 121 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = E;code = 69 char = S;code = 83 char = T;code = 84 char = -;code = 45 char = S;code = 83 char = S;code = 83 char = R;code = 82 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = l;code = 108 char = u;code = 117 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = 2;code = 50 char = 0;code = 48 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = 3;code = 51 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = 8;code = 56 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = k;code = 107 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = l;code = 108 char = u;code = 117 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 1;code = 49 char = 3;code = 51 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = C;code = 67 char = .;code = 46 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = a;code = 97 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = i;code = 105 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = %;code = 37 char = );code = 41 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = l;code = 108 char = y;code = 121 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = p;code = 112 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = k;code = 107 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = v;code = 118 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = a;code = 97 char = x;code = 120 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = u;code = 117 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
Publication: Mol. Biotechnol. 2010 Feb ;44(2)::140-7. :doi: 10.1007/s12033-009-9222-4.
Publication Date: 2010 Feb
Study Author(s): Siju, S;Dhanya, K;Syamkumar, S;Sasikumar, B;Sheeja, T E;Bhat, A I;Parthasarathy, V A;
Institution: Indian Institute of Spices Research, Calicut, Kerala, India.
Shortcut link to this study:
Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
PMID: 19924572