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char = e;code = 101
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char = e;code = 101
char = l;code = 108
char = o;code = 111
char = p;code = 112
char = m;code = 109
char = e;code = 101
char = n;code = 110
char = t;code = 116
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char = a;code = 97
char = r;code = 114
char = a;code = 97
char = c;code = 99
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char = e;code = 101
char = r;code = 114
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char = z;code = 122
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = a;code = 97
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char = ;code = 32
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char = s;code = 115
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char = e;code = 101
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char = m;code = 109
char = p;code = 112
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char = i;code = 105
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = p;code = 112
char = o;code = 111
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char = m;code = 109
char = o;code = 111
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char = p;code = 112
char = h;code = 104
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = s;code = 115
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = r;code = 114
char = k;code = 107
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = q;code = 113
char = u;code = 117
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = a;code = 97
char = g;code = 103
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = m;code = 109
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = (;code = 40
char = C;code = 67
char = u;code = 117
char = r;code = 114
char = c;code = 99
char = u;code = 117
char = m;code = 109
char = a;code = 97
char = ;code = 32
char = l;code = 108
char = o;code = 111
char = n;code = 110
char = g;code = 103
char = a;code = 97
char = ;code = 32
char = L;code = 76
char = .;code = 46
char = );code = 41
char = .;code = 46
result sql = Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).
Development, characterization and cross species amplification of polymorphic microsatellite markers from expressed sequence tags of turmeric (Curcuma longa L.).
char = E;code = 69
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char = p;code = 112
char = r;code = 114
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char = s;code = 115
char = e;code = 101
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char = ;code = 32
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char = e;code = 101
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char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = a;code = 97
char = g;code = 103
char = s;code = 115
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char = (;code = 40
char = E;code = 69
char = S;code = 83
char = T;code = 84
char = s;code = 115
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char = ;code = 32
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = m;code = 109
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = (;code = 40
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char = r;code = 114
char = c;code = 99
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char = a;code = 97
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char = o;code = 111
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char = g;code = 103
char = a;code = 97
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char = ;code = 32
char = w;code = 119
char = e;code = 101
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char = e;code = 101
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char = s;code = 115
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char = d;code = 100
char = ;code = 32
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char = o;code = 111
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char = e;code = 101
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char = s;code = 115
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char = e;code = 101
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char = n;code = 110
char = g;code = 103
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char = o;code = 111
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char = t;code = 116
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char = d;code = 100
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = e;code = 101
char = q;code = 113
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char = e;code = 101
char = n;code = 110
char = c;code = 99
char = y;code = 121
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = C;code = 67
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = s;code = 115
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char = I;code = 73
char = ;code = 32
char = (;code = 40
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char = y;code = 121
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char = a;code = 97
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char = i;code = 105
char = a;code = 97
char = b;code = 98
char = l;code = 108
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char = ;code = 32
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char = m;code = 109
char = p;code = 112
char = l;code = 108
char = e;code = 101
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char = s;code = 115
char = e;code = 101
char = q;code = 113
char = u;code = 117
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = r;code = 114
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char = p;code = 112
char = e;code = 101
char = a;code = 97
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = S;code = 83
char = S;code = 83
char = R;code = 82
char = s;code = 115
char = );code = 41
char = .;code = 46
char = ;code = 32
char = A;code = 65
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = t;code = 116
char = a;code = 97
char = l;code = 108
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char = ;code = 32
char = 2;code = 50
char = 3;code = 51
char = 1;code = 49
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char = m;code = 109
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char = c;code = 99
char = r;code = 114
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char = a;code = 97
char = t;code = 116
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = e;code = 101
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char = r;code = 114
char = e;code = 101
char = p;code = 112
char = e;code = 101
char = a;code = 97
char = t;code = 116
char = s;code = 115
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char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = d;code = 100
char = e;code = 101
char = t;code = 116
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = ;code = 32
char = 1;code = 49
char = 2;code = 50
char = ,;code = 44
char = 5;code = 53
char = 9;code = 57
char = 3;code = 51
char = ;code = 32
char = E;code = 69
char = S;code = 83
char = T;code = 84
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = q;code = 113
char = u;code = 117
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = s;code = 115
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char = o;code = 111
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char = ;code = 32
char = t;code = 116
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char = e;code = 101
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char = f;code = 102
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char = r;code = 114
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char = d;code = 100
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char = n;code = 110
char = d;code = 100
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = y;code = 121
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char = e;code = 101
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char = i;code = 105
char = m;code = 109
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char = n;code = 110
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = a;code = 97
char = v;code = 118
char = e;code = 101
char = r;code = 114
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char = g;code = 103
char = e;code = 101
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char = d;code = 100
char = e;code = 101
char = n;code = 110
char = s;code = 115
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = C;code = 67
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = ;code = 32
char = I;code = 73
char = ;code = 32
char = S;code = 83
char = S;code = 83
char = R;code = 82
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = c;code = 99
char = o;code = 111
char = u;code = 117
char = n;code = 110
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = o;code = 111
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = S;code = 83
char = S;code = 83
char = R;code = 82
char = ;code = 32
char = p;code = 112
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = 1;code = 49
char = 7;code = 55
char = .;code = 46
char = 9;code = 57
char = 6;code = 54
char = ;code = 32
char = k;code = 107
char = b;code = 98
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = E;code = 69
char = S;code = 83
char = T;code = 84
char = .;code = 46
char = ;code = 32
char = M;code = 77
char = o;code = 111
char = n;code = 110
char = o;code = 111
char = n;code = 110
char = u;code = 117
char = c;code = 99
char = l;code = 108
char = e;code = 101
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char = e;code = 101
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char = s;code = 115
char = t;code = 116
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char = a;code = 97
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char = d;code = 100
char = a;code = 97
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char = t;code = 116
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char = c;code = 99
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = s;code = 115
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
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char = s;code = 115
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char = e;code = 101
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = t;code = 116
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char = p;code = 112
char = e;code = 101
char = a;code = 97
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char = m;code = 109
char = e;code = 101
char = r;code = 114
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char = c;code = 99
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char = E;code = 69
char = S;code = 83
char = T;code = 84
char = s;code = 115
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char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = o;code = 111
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
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char = n;code = 110
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char = c;code = 99
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char = e;code = 101
char = o;code = 111
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char = i;code = 105
char = d;code = 100
char = e;code = 101
char = s;code = 115
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char = ;code = 32
char = A;code = 65
char = ;code = 32
char = r;code = 114
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char = u;code = 117
char = s;code = 115
char = t;code = 116
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = t;code = 116
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char = o;code = 111
char = f;code = 102
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char = 1;code = 49
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char = ;code = 32
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char = l;code = 108
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char = h;code = 104
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = E;code = 69
char = S;code = 83
char = T;code = 84
char = -;code = 45
char = S;code = 83
char = S;code = 83
char = R;code = 82
char = s;code = 115
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char = i;code = 105
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char = s;code = 115
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
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char = l;code = 108
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char = e;code = 101
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char = l;code = 108
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char = e;code = 101
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char = e;code = 101
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char = a;code = 97
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char = f;code = 102
char = i;code = 105
char = r;code = 114
char = m;code = 109
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char = n;code = 110
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char = h;code = 104
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char = g;code = 103
char = h;code = 104
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result sql = Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.

Publication: Mol. Biotechnol. 2010 Feb ;44(2)::140-7. :doi: 10.1007/s12033-009-9222-4.
Publication Date: 2010 Feb
Study Author(s): Siju, S;Dhanya, K;Syamkumar, S;Sasikumar, B;Sheeja, T E;Bhat, A I;Parthasarathy, V A;
Institution: Indian Institute of Spices Research, Calicut, Kerala, India.
Shortcut link to this study:
Expressed sequence tags (ESTs) from turmeric (Curcuma longa L.) were used for the screening of type and frequency of Class I (hypervariable) simple sequence repeats (SSRs). A total of 231 microsatellite repeats were detected from 12,593 EST sequences of turmeric after redundancy elimination. The average density of Class I SSRs accounts to one SSR per 17.96 kb of EST. Mononucleotides were the most abundant class of microsatellite repeat in turmeric ESTs followed by trinucleotides. A robust set of 17 polymorphic EST-SSRs were developed and used for evaluating 20 turmeric accessions. The number of alleles detected ranged from 3 to 8 per loci. The developed markers were also evaluated in 13 related species of C. longa confirming high rate (100%) of cross species transferability. The polymorphic microsatellite markers generated from this study could be used for genetic diversity analysis and resolving the taxonomic confusion prevailing in the genus.
PMID: 19924572

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