char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = C;code = 67 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = t;code = 116 char = 7;code = 55 char = /;code = 47 char = 9;code = 57 char = -;code = 45 char = K;code = 75 char = M;code = 77 char = T;code = 84 char = 7;code = 55 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = -;code = 45 char = 1;code = 49 char = ;code = 32 char = T;code = 84 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 result sql = The Cellular lysine methyltransferase Set7/9-KMT7 binds HIV-1 TAR RNA, monomethylates the viral transactivator Tat, and enhances HIV transcription.
The Cellular lysine methyltransferase Set7/9-KMT7 binds HIV-1 TAR RNA, monomethylates the viral transactivator Tat, and enhances HIV transcription.char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = -;code = 45 char = 1;code = 49 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = P;code = 80 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = -;code = 45 char = t;code = 116 char = u;code = 117 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = T;code = 84 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = -;code = 45 char = l;code = 108 char = o;code = 111 char = o;code = 111 char = p;code = 112 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = 5;code = 53 char = ';code = 39 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = H;code = 72 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = f;code = 102 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = t;code = 116 char = 7;code = 55 char = /;code = 47 char = 9;code = 57 char = ;code = 32 char = (;code = 40 char = K;code = 75 char = M;code = 77 char = T;code = 84 char = 7;code = 55 char = );code = 41 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = t;code = 116 char = 7;code = 55 char = /;code = 47 char = 9;code = 57 char = -;code = 45 char = K;code = 75 char = M;code = 77 char = T;code = 84 char = 7;code = 55 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = v;code = 118 char = o;code = 111 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = 5;code = 53 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = h;code = 104 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = d;code = 100 char = u;code = 117 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = -;code = 45 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = K;code = 75 char = n;code = 110 char = o;code = 111 char = c;code = 99 char = k;code = 107 char = d;code = 100 char = o;code = 111 char = w;code = 119 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = t;code = 116 char = 7;code = 55 char = /;code = 47 char = 9;code = 57 char = -;code = 45 char = K;code = 75 char = M;code = 77 char = T;code = 84 char = 7;code = 55 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = p;code = 112 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = H;code = 72 char = I;code = 73 char = V;code = 86 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = K;code = 75 char = 5;code = 53 char = 1;code = 49 char = A;code = 65 char = );code = 41 char = .;code = 46 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = t;code = 116 char = 7;code = 55 char = /;code = 47 char = 9;code = 57 char = -;code = 45 char = K;code = 75 char = M;code = 77 char = T;code = 84 char = 7;code = 55 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = T;code = 84 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = e;code = 101 char = l;code = 108 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = x;code = 120 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = P;code = 80 char = -;code = 45 char = T;code = 84 char = E;code = 69 char = F;code = 70 char = b;code = 98 char = .;code = 46 char = ;code = 32 char = O;code = 79 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = S;code = 83 char = e;code = 101 char = t;code = 116 char = 7;code = 55 char = /;code = 47 char = 9;code = 57 char = -;code = 45 char = K;code = 75 char = M;code = 77 char = T;code = 84 char = 7;code = 55 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = u;code = 117 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = p;code = 112 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = y;code = 121 char = c;code = 99 char = l;code = 108 char = e;code = 101 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = The Tat protein of HIV-1 plays an essential role in HIV gene expression by promoting efficient elongation of viral transcripts. Posttranslational modifications of Tat fine-tune interactions of Tat with cellular cofactors and TAR RNA, a stem-loop structure at the 5' ends of viral transcripts. Here, we identify the lysine methyltransferase Set7/9 (KMT7) as a coactivator of HIV transcription. Set7/9-KMT7 associates with the HIV promoter in vivo and monomethylates lysine 51, a highly conserved residue located in the RNA-binding domain of Tat. Knockdown of Set7/9-KMT7 suppresses Tat transactivation of the HIV promoter, but does not affect the transcriptional activity of methylation-deficient Tat (K51A). Set7/9-KMT7 binds TAR RNA by itself and in complex with Tat and the positive transcription elongation factor P-TEFb. Our findings uncover a positive role for Set7/9-KMT7 and Tat methylation during early steps of the Tat transactivation cycle.
Publication: Cell Host Microbe 2010 Mar 18 ;7(3)::234-44. :doi: 10.1016/j.chom.2010.02.005.
Publication Date: 2010 Mar 18
Study Author(s): Pagans, Sara;Kauder, Steven E;Kaehlcke, Katrin;Sakane, Naoki;Schroeder, Sebastian;Dormeyer, Wilma;Trievel, Raymond C;Verdin, Eric;Schnolzer, Martina;Ott, Melanie;
Institution: Gladstone Institute of Virology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA.
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The Tat protein of HIV-1 plays an essential role in HIV gene expression by promoting efficient elongation of viral transcripts. Posttranslational modifications of Tat fine-tune interactions of Tat with cellular cofactors and TAR RNA, a stem-loop structure at the 5' ends of viral transcripts. Here, we identify the lysine methyltransferase Set7/9 (KMT7) as a coactivator of HIV transcription. Set7/9-KMT7 associates with the HIV promoter in vivo and monomethylates lysine 51, a highly conserved residue located in the RNA-binding domain of Tat. Knockdown of Set7/9-KMT7 suppresses Tat transactivation of the HIV promoter, but does not affect the transcriptional activity of methylation-deficient Tat (K51A). Set7/9-KMT7 binds TAR RNA by itself and in complex with Tat and the positive transcription elongation factor P-TEFb. Our findings uncover a positive role for Set7/9-KMT7 and Tat methylation during early steps of the Tat transactivation cycle.
PMID: 20227666