char = I;code = 73 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = O;code = 79 char = r;code = 114 char = g;code = 103 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = E;code = 69 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = C;code = 67 char = o;code = 111 char = -;code = 45 char = o;code = 111 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = D;code = 68 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = O;code = 79 char = E;code = 69 char = C;code = 67 char = D;code = 68 char = );code = 41 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = s;code = 115 char = -;code = 45 char = ;code = 32 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = i;code = 105 char = s;code = 115 char = h;code = 104 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = (;code = 40 char = r;code = 114 char = r;code = 114 char = A;code = 65 char = R;code = 82 char = );code = 41 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = .;code = 46 result sql = In vitro study of Organization for Economic Co-operation and Development (OECD) endocrine disruptor screening and testing methods- establishment of a recombinant rat androgen receptor (rrAR) binding assay.
In vitro study of Organization for Economic Co-operation and Development (OECD) endocrine disruptor screening and testing methods- establishment of a recombinant rat androgen receptor (rrAR) binding assay.char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = e;code = 101 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = (;code = 40 char = A;code = 65 char = R;code = 82 char = );code = 41 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = a;code = 97 char = b;code = 98 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = c;code = 99 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = D;code = 68 char = s;code = 115 char = );code = 41 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = s;code = 115 char = y;code = 121 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = 1;code = 49 char = 8;code = 56 char = 8;code = 56 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = (;code = 40 char = r;code = 114 char = r;code = 114 char = A;code = 65 char = R;code = 82 char = );code = 41 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = L;code = 76 char = e;code = 101 char = x;code = 120 char = i;code = 105 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = F;code = 70 char = r;code = 114 char = e;code = 101 char = y;code = 121 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = g;code = 103 char = e;code = 101 char = r;code = 114 char = ';code = 39 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = d;code = 100 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = k;code = 107 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = I;code = 73 char = C;code = 67 char = (;code = 40 char = 5;code = 53 char = 0;code = 48 char = );code = 41 char = );code = 41 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = n;code = 110 char = b;code = 98 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = 1;code = 49 char = .;code = 46 char = 3;code = 51 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 8;code = 56 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 1;code = 49 char = 3;code = 51 char = 8;code = 56 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = h;code = 104 char = y;code = 121 char = d;code = 100 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = D;code = 68 char = H;code = 72 char = T;code = 84 char = );code = 41 char = ;code = 32 char = 1;code = 49 char = .;code = 46 char = 8;code = 56 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 8;code = 56 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = 5;code = 53 char = .;code = 46 char = 7;code = 55 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 8;code = 56 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 3;code = 51 char = 1;code = 49 char = .;code = 46 char = 6;code = 54 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = y;code = 121 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = (;code = 40 char = N;code = 78 char = P;code = 80 char = );code = 41 char = ;code = 32 char = 1;code = 49 char = .;code = 46 char = 3;code = 51 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 5;code = 53 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 1;code = 49 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = s;code = 115 char = p;code = 112 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = A;code = 65 char = ;code = 32 char = (;code = 40 char = B;code = 66 char = P;code = 80 char = A;code = 65 char = );code = 41 char = ;code = 32 char = 1;code = 49 char = .;code = 46 char = 1;code = 49 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 1;code = 49 char = 6;code = 54 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = 3;code = 51 char = .;code = 46 char = 1;code = 49 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 0;code = 48 char = 5;code = 53 char = 8;code = 56 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = 6;code = 54 char = .;code = 46 char = 2;code = 50 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 0;code = 48 char = 2;code = 50 char = 9;code = 57 char = );code = 41 char = ;code = 32 char = >;code = 62 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = b;code = 98 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = 9;code = 57 char = .;code = 46 char = 7;code = 55 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = (;code = 40 char = -;code = 45 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = (;code = 40 char = R;code = 82 char = B;code = 66 char = A;code = 65 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 0;code = 48 char = 0;code = 48 char = 1;code = 49 char = 9;code = 57 char = );code = 41 char = .;code = 46 char = ;code = 32 char = H;code = 72 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = ,;code = 44 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = (;code = 40 char = n;code = 110 char = -;code = 45 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = y;code = 121 char = l;code = 108 char = );code = 41 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = D;code = 68 char = B;code = 66 char = P;code = 80 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = (;code = 40 char = 2;code = 50 char = -;code = 45 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = h;code = 104 char = e;code = 101 char = x;code = 120 char = y;code = 121 char = l;code = 108 char = );code = 41 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = D;code = 68 char = E;code = 69 char = H;code = 72 char = P;code = 80 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = k;code = 107 char = n;code = 110 char = o;code = 111 char = w;code = 119 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = -;code = 45 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = .;code = 46 char = ;code = 32 char = O;code = 79 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = ;code = 32 char = A;code = 65 char = R;code = 82 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = a;code = 97 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = E;code = 69 char = D;code = 68 char = s;code = 115 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = The androgen receptor (AR) binding assay can be used to determine the ability of probable endocrine disruptors (EDs) to compete with synthetic androgen methyltrienolone (R1881) for binding to recombinant rat AR (rrAR). In this study, we assessed AR binding of various chemicals using Lexius Freyberger's method. The rank of relative binding affinity (RBA, IC(50)) on the tested chemicals was trenbolone 1.3 x 10(-8) M (RBA 138) > dihydrotesterone (DHT) 1.8 x 10(-8) M (RBA 100) > methyl testosterone 5.7 x 10(-8) M (RBA 31.6) > nonylphenol (NP) 1.3 x 10(-5) M (RBA 0.14) > bisphenol A (BPA) 1.1 x 10(-4) M (RBA 0.016) > isobutyl paraben 3.1 x 10(-4) M (RBA 0.0058) > butyl paraben 6.2 x 10(-4) M (RBA 0.0029) > propyl paraben 9.7 x 10(-4) M (RBA 0.0019). However, di(n-butyl) phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP), known anti-androgenic chemicals, did not show any significant AR binding activity. Our data suggests that in vitro AR binding assay may be useful as a screening tool for potential EDs.
Publication: J Toxicol Sci 2010 Apr ;35(2)::239-43.
Publication Date: 2010 Apr
Study Author(s): Kim, Tae Sung;Yoon, Chang Yong;Jung, Ki Kyung;Kim, Soon Sun;Kang, Il Hyun;Baek, Jung Hee;Jo, Min Soo;Kim, Hyung Sik;Kang, Tae Seok;
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The androgen receptor (AR) binding assay can be used to determine the ability of probable endocrine disruptors (EDs) to compete with synthetic androgen methyltrienolone (R1881) for binding to recombinant rat AR (rrAR). In this study, we assessed AR binding of various chemicals using Lexius Freyberger's method. The rank of relative binding affinity (RBA, IC(50)) on the tested chemicals was trenbolone 1.3 x 10(-8) M (RBA 138) > dihydrotesterone (DHT) 1.8 x 10(-8) M (RBA 100) > methyl testosterone 5.7 x 10(-8) M (RBA 31.6) > nonylphenol (NP) 1.3 x 10(-5) M (RBA 0.14) > bisphenol A (BPA) 1.1 x 10(-4) M (RBA 0.016) > isobutyl paraben 3.1 x 10(-4) M (RBA 0.0058) > butyl paraben 6.2 x 10(-4) M (RBA 0.0029) > propyl paraben 9.7 x 10(-4) M (RBA 0.0019). However, di(n-butyl) phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP), known anti-androgenic chemicals, did not show any significant AR binding activity. Our data suggests that in vitro AR binding assay may be useful as a screening tool for potential EDs.
PMID: 20371976