char = M;code = 77 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = q;code = 113 char = u;code = 117 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = -;code = 45 char = 1;code = 49 char = :;code = 58 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = d;code = 100 char = y;code = 121 char = n;code = 110 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 result sql = Molecular mechanisms of how mercury inhibits water permeation through aquaporin-1: understanding by molecular dynamics simulation.
Molecular mechanisms of how mercury inhibits water permeation through aquaporin-1: understanding by molecular dynamics simulation.char = A;code = 65 char = q;code = 113 char = u;code = 117 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = );code = 41 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = n;code = 110 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = -;code = 45 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = l;code = 108 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = -;code = 45 char = d;code = 100 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = 8;code = 56 char = 9;code = 57 char = ;code = 32 char = d;code = 100 char = u;code = 117 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = t;code = 116 char = o;code = 111 char = o;code = 111 char = d;code = 100 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = h;code = 104 char = i;code = 105 char = b;code = 98 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = 4;code = 52 char = 0;code = 48 char = ;code = 32 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = d;code = 100 char = y;code = 121 char = n;code = 110 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = (;code = 40 char = H;code = 72 char = g;code = 103 char = -;code = 45 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = (;code = 40 char = F;code = 70 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = );code = 41 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = H;code = 72 char = g;code = 103 char = -;code = 45 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = 9;code = 57 char = 1;code = 49 char = ;code = 32 char = (;code = 40 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = 8;code = 56 char = 9;code = 57 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = h;code = 104 char = u;code = 117 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = -;code = 45 char = S;code = 83 char = H;code = 72 char = g;code = 103 char = +;code = 43 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = .;code = 46 char = ;code = 32 char = D;code = 68 char = u;code = 117 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = ;code = 32 char = n;code = 110 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = 2;code = 50 char = 3;code = 51 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = F;code = 70 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = H;code = 72 char = g;code = 103 char = -;code = 45 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = .;code = 46 char = ;code = 32 char = M;code = 77 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = a;code = 97 char = p;code = 112 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = d;code = 100 char = u;code = 117 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = /;code = 47 char = R;code = 82 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = F;code = 70 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = c;code = 99 char = k;code = 107 char = b;code = 98 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = y;code = 121 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = G;code = 71 char = l;code = 108 char = y;code = 121 char = 1;code = 49 char = 9;code = 57 char = 0;code = 48 char = ,;code = 44 char = ;code = 32 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = 9;code = 57 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = G;code = 71 char = l;code = 108 char = y;code = 121 char = 1;code = 49 char = 9;code = 57 char = 2;code = 50 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = r;code = 114 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = l;code = 108 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = H;code = 72 char = g;code = 103 char = -;code = 45 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = H;code = 72 char = g;code = 103 char = +;code = 43 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = 9;code = 57 char = 1;code = 49 char = -;code = 45 char = S;code = 83 char = H;code = 72 char = g;code = 103 char = +;code = 43 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = w;code = 119 char = a;code = 97 char = r;code = 114 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = u;code = 117 char = t;code = 116 char = s;code = 115 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = c;code = 99 char = k;code = 107 char = b;code = 98 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = x;code = 120 char = y;code = 121 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = G;code = 71 char = l;code = 108 char = y;code = 121 char = 1;code = 49 char = 9;code = 57 char = 0;code = 48 char = ,;code = 44 char = ;code = 32 char = C;code = 67 char = y;code = 121 char = s;code = 115 char = 1;code = 49 char = 9;code = 57 char = 1;code = 49 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = G;code = 71 char = l;code = 108 char = y;code = 121 char = 1;code = 49 char = 9;code = 57 char = 2;code = 50 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = c;code = 99 char = a;code = 97 char = m;code = 109 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = r;code = 114 char = g;code = 103 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = /;code = 47 char = R;code = 82 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = a;code = 97 char = p;code = 112 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = r;code = 114 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = A;code = 65 char = Q;code = 81 char = P;code = 80 char = 1;code = 49 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = /;code = 47 char = R;code = 82 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Aquaporin (AQP) functions as a water-conducting pore. Mercury inhibits the water permeation through AQP. Although site-directed mutagenesis has shown that mercury binds to Cys189 during the inhibition process, it is not fully understood how this inhibits the water permeation through AQP1. We carried out 40 ns molecular dynamics simulations of bovine AQP1 tetramer with mercury (Hg-AQP1) or without mercury (Free AQP1). In Hg-AQP1, Cys191 (Cys189 in human AQP1) is converted to Cys-SHg+ in each monomer. During each last 10 ns, we observed water permeation events occurred 23 times in Free AQP1 and never in Hg-AQP1. Mercury binding did not influence the whole structure, but did induce a collapse in the orientation of several residues at the ar/R region. In Free AQP1, backbone oxygen atoms of Gly190, Cys191, and Gly192 lined, and were oriented to, the surface of the water pore channel. In Hg-AQP1, however, the SHg+ of Cys191-SHg+ was oriented toward the outside of the water pore. As a result, the backbone oxygen atoms of Gly190, Cys191, and Gly192 became disorganized and the ar/R region collapsed, thereby obstructing the permeation of water. We suggest that mercury disrupts the water pore of AQP1 through local conformational changes in the ar/R region.
Publication: Biophys. J. 2010 Apr 21 ;98(8)::1512-9. :doi: 10.1016/j.bpj.2009.12.4310.
Publication Date: 2010 Apr 21
Study Author(s): Hirano, Yoshinori;Okimoto, Noriaki;Kadohira, Ikuko;Suematsu, Makoto;Yasuoka, Kenji;Yasui, Masato;
Institution: Department of Pharmacology, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan.
[email protected]
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Aquaporin (AQP) functions as a water-conducting pore. Mercury inhibits the water permeation through AQP. Although site-directed mutagenesis has shown that mercury binds to Cys189 during the inhibition process, it is not fully understood how this inhibits the water permeation through AQP1. We carried out 40 ns molecular dynamics simulations of bovine AQP1 tetramer with mercury (Hg-AQP1) or without mercury (Free AQP1). In Hg-AQP1, Cys191 (Cys189 in human AQP1) is converted to Cys-SHg+ in each monomer. During each last 10 ns, we observed water permeation events occurred 23 times in Free AQP1 and never in Hg-AQP1. Mercury binding did not influence the whole structure, but did induce a collapse in the orientation of several residues at the ar/R region. In Free AQP1, backbone oxygen atoms of Gly190, Cys191, and Gly192 lined, and were oriented to, the surface of the water pore channel. In Hg-AQP1, however, the SHg+ of Cys191-SHg+ was oriented toward the outside of the water pore. As a result, the backbone oxygen atoms of Gly190, Cys191, and Gly192 became disorganized and the ar/R region collapsed, thereby obstructing the permeation of water. We suggest that mercury disrupts the water pore of AQP1 through local conformational changes in the ar/R region.
PMID: 20409470