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char = n;code = 110
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char = s;code = 115
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char = u;code = 117
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char = i;code = 105
char = o;code = 111
char = x;code = 120
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = .;code = 46
result sql = Disinfection kinetics of murine norovirus using chlorine and chlorine dioxide.
Disinfection kinetics of murine norovirus using chlorine and chlorine dioxide.
char = W;code = 87
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char = e;code = 101
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char = d;code = 100
char = i;code = 105
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char = i;code = 105
char = n;code = 110
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = e;code = 101
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char = f;code = 102
char = i;code = 105
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char = i;code = 105
char = e;code = 101
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char = ;code = 32
char = c;code = 99
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char = l;code = 108
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char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
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char = C;code = 67
char = l;code = 108
char = O;code = 79
char = (;code = 40
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char = );code = 41
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char = s;code = 115
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char = s;code = 115
char = ;code = 32
char = (;code = 40
char = M;code = 77
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char = V;code = 86
char = );code = 41
char = ;code = 32
char = a;code = 97
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char = d;code = 100
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char = c;code = 99
char = o;code = 111
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char = h;code = 104
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char = g;code = 103
char = e;code = 101
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char = M;code = 77
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char = a;code = 97
char = s;code = 115
char = ;code = 32
char = s;code = 115
char = u;code = 117
char = r;code = 114
char = r;code = 114
char = o;code = 111
char = g;code = 103
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = h;code = 104
char = u;code = 117
char = m;code = 109
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = n;code = 110
char = o;code = 111
char = r;code = 114
char = o;code = 111
char = v;code = 118
char = i;code = 105
char = r;code = 114
char = u;code = 117
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = E;code = 69
char = x;code = 120
char = p;code = 112
char = e;code = 101
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char = i;code = 105
char = m;code = 109
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = w;code = 119
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char = e;code = 101
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char = e;code = 101
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char = i;code = 105
char = n;code = 110
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char = o;code = 111
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char = i;code = 105
char = d;code = 100
char = a;code = 97
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = m;code = 109
char = a;code = 97
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char = d;code = 100
char = -;code = 45
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char = r;code = 114
char = e;code = 101
char = e;code = 101
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char = b;code = 98
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char = f;code = 102
char = f;code = 102
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = (;code = 40
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char = H;code = 72
char = ;code = 32
char = 7;code = 55
char = .;code = 46
char = 2;code = 50
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char = ;code = 32
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = 5;code = 53
char = ;code = 32
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char = g;code = 103
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char = e;code = 101
char = e;code = 101
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char = C;code = 67
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char = d;code = 100
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char = e;code = 101
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = C;code = 67
char = .;code = 46
char = ;code = 32
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = T;code = 84
char = H;code = 72
char = E;code = 69
char = ;code = 32
char = E;code = 69
char = X;code = 88
char = T;code = 84
char = E;code = 69
char = N;code = 78
char = T;code = 84
char = ;code = 32
char = O;code = 79
char = F;code = 70
char = ;code = 32
char = V;code = 86
char = I;code = 73
char = R;code = 82
char = U;code = 85
char = S;code = 83
char = ;code = 32
char = I;code = 73
char = N;code = 78
char = A;code = 65
char = C;code = 67
char = T;code = 84
char = I;code = 73
char = V;code = 86
char = A;code = 65
char = T;code = 84
char = I;code = 73
char = O;code = 79
char = N;code = 78
char = ;code = 32
char = B;code = 66
char = Y;code = 89
char = ;code = 32
char = A;code = 65
char = ;code = 32
char = D;code = 68
char = I;code = 73
char = S;code = 83
char = I;code = 73
char = N;code = 78
char = F;code = 70
char = E;code = 69
char = C;code = 67
char = T;code = 84
char = A;code = 65
char = N;code = 78
char = T;code = 84
char = ;code = 32
char = W;code = 87
char = A;code = 65
char = S;code = 83
char = ;code = 32
char = Q;code = 81
char = U;code = 85
char = A;code = 65
char = N;code = 78
char = T;code = 84
char = I;code = 73
char = F;code = 70
char = I;code = 73
char = E;code = 69
char = D;code = 68
char = ;code = 32
char = U;code = 85
char = S;code = 83
char = I;code = 73
char = N;code = 78
char = G;code = 71
char = ;code = 32
char = T;code = 84
char = H;code = 72
char = R;code = 82
char = E;code = 69
char = E;code = 69
char = ;code = 32
char = D;code = 68
char = I;code = 73
char = F;code = 70
char = F;code = 70
char = E;code = 69
char = R;code = 82
char = E;code = 69
char = N;code = 78
char = T;code = 84
char = ;code = 32
char = A;code = 65
char = N;code = 78
char = A;code = 65
char = L;code = 76
char = Y;code = 89
char = T;code = 84
char = I;code = 73
char = C;code = 67
char = A;code = 65
char = L;code = 76
char = ;code = 32
char = M;code = 77
char = E;code = 69
char = T;code = 84
char = H;code = 72
char = O;code = 79
char = D;code = 68
char = S;code = 83
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = ;code = 32
char = p;code = 112
char = l;code = 108
char = a;code = 97
char = q;code = 113
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char = e;code = 101
char = ,;code = 44
char = ;code = 32
char = s;code = 115
char = h;code = 104
char = o;code = 111
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char = t;code = 116
char = ;code = 32
char = t;code = 116
char = e;code = 101
char = m;code = 109
char = p;code = 112
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = a;code = 97
char = l;code = 108
char = -;code = 45
char = t;code = 116
char = i;code = 105
char = m;code = 109
char = e;code = 101
char = ;code = 32
char = T;code = 84
char = a;code = 97
char = q;code = 113
char = M;code = 77
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = v;code = 118
char = e;code = 101
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char = s;code = 115
char = e;code = 101
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char = t;code = 116
char = r;code = 114
char = a;code = 97
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char = s;code = 115
char = c;code = 99
char = r;code = 114
char = i;code = 105
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char = t;code = 116
char = a;code = 97
char = s;code = 115
char = e;code = 101
char = -;code = 45
char = p;code = 112
char = o;code = 111
char = l;code = 108
char = y;code = 121
char = m;code = 109
char = e;code = 101
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char = a;code = 97
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char = e;code = 101
char = ;code = 32
char = c;code = 99
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char = a;code = 97
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char = a;code = 97
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char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = (;code = 40
char = R;code = 82
char = T;code = 84
char = -;code = 45
char = P;code = 80
char = C;code = 67
char = R;code = 82
char = );code = 41
char = ,;code = 44
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char = e;code = 101
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char = T;code = 84
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char = C;code = 67
char = R;code = 82
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char = a;code = 97
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char = s;code = 115
char = a;code = 97
char = y;code = 121
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char = i;code = 105
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = M;code = 77
char = N;code = 78
char = V;code = 86
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char = b;code = 98
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = p;code = 112
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char = e;code = 101
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char = a;code = 97
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char = s;code = 115
char = a;code = 97
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char = f;code = 102
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char = m;code = 109
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char = o;code = 111
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char = V;code = 86
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char = a;code = 97
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result sql = We determined the disinfection efficiency of chlorine and chlorine dioxide (ClO(2)) using murine norovirus (MNV) and coliphage MS2 as surrogates for human norovirus. Experiments were performed in oxidant demand-free buffer (pH 7.2) at 5 degrees C and 20 degrees C. THE EXTENT OF VIRUS INACTIVATION BY A DISINFECTANT WAS QUANTIFIED USING THREE DIFFERENT ANALYTICAL METHODS: plaque, short template real-time TaqMan reverse transcriptase-polymerase chain reaction (RT-PCR), and long template RT-PCR assays. Rapid inactivation of MNV by both chlorine and chlorine dioxide was observed by the plaque assay. According to the efficiency factor Hom model, Ct values of 0.314mg/Lmin and 0.247mg/Lmin were required for a 4-log reduction of MNV at 5 degrees C by chlorine and chlorine dioxide, respectively. Lower Ct values were required at 20 degrees C. Both long template and short template RT-PCR assays significantly underestimated the virus inactivation compared to the plaque assay. Our study demonstrates that adequate treatment of water with either chlorine or ClO(2) is likely to effectively control the waterborne transmission of human norovirus.

Publication: Water Res. 2010 May ;44(10)::3243-51. :doi: 10.1016/j.watres.2010.03.003.
Publication Date: 2010 May
Study Author(s): Lim, Mi Young;Kim, Ju-Mi;Ko, Gwangpyo;
Institution: Department of Environmental Health, and Institute of Health and Environment, School of Public Health, Seoul National University, 599 Kwanak-ro Kwanak-gu, Seoul 151-747, Republic of Korea.
Shortcut link to this study:
We determined the disinfection efficiency of chlorine and chlorine dioxide (ClO(2)) using murine norovirus (MNV) and coliphage MS2 as surrogates for human norovirus. Experiments were performed in oxidant demand-free buffer (pH 7.2) at 5 degrees C and 20 degrees C. THE EXTENT OF VIRUS INACTIVATION BY A DISINFECTANT WAS QUANTIFIED USING THREE DIFFERENT ANALYTICAL METHODS: plaque, short template real-time TaqMan reverse transcriptase-polymerase chain reaction (RT-PCR), and long template RT-PCR assays. Rapid inactivation of MNV by both chlorine and chlorine dioxide was observed by the plaque assay. According to the efficiency factor Hom model, Ct values of 0.314mg/Lmin and 0.247mg/Lmin were required for a 4-log reduction of MNV at 5 degrees C by chlorine and chlorine dioxide, respectively. Lower Ct values were required at 20 degrees C. Both long template and short template RT-PCR assays significantly underestimated the virus inactivation compared to the plaque assay. Our study demonstrates that adequate treatment of water with either chlorine or ClO(2) is likely to effectively control the waterborne transmission of human norovirus.
PMID: 20356616

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