char = A;code = 65
char = n;code = 110
char = ;code = 32
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = u;code = 117
char = r;code = 114
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = e;code = 101
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = :;code = 58
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = e;code = 101
char = n;code = 110
char = d;code = 100
char = o;code = 111
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = o;code = 111
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = f;code = 102
char = a;code = 97
char = t;code = 116
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = i;code = 105
char = d;code = 100
char = ;code = 32
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = e;code = 101
char = n;code = 110
char = h;code = 104
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = 2;code = 50
char = -;code = 45
char = a;code = 97
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = h;code = 104
char = i;code = 105
char = d;code = 100
char = o;code = 111
char = n;code = 110
char = o;code = 111
char = y;code = 121
char = l;code = 108
char = -;code = 45
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = n;code = 110
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = n;code = 110
char = o;code = 111
char = i;code = 105
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = .;code = 46
result sql = An entourage effect: inactive endogenous fatty acid glycerol esters enhance 2-arachidonoyl-glycerol cannabinoid activity.
An entourage effect: inactive endogenous fatty acid glycerol esters enhance 2-arachidonoyl-glycerol cannabinoid activity.
char = 2;code = 50
char = -;code = 45
char = A;code = 65
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = h;code = 104
char = i;code = 105
char = d;code = 100
char = o;code = 111
char = n;code = 110
char = o;code = 111
char = y;code = 121
char = l;code = 108
char = -;code = 45
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = (;code = 40
char = 2;code = 50
char = -;code = 45
char = A;code = 65
char = r;code = 114
char = a;code = 97
char = -;code = 45
char = G;code = 71
char = I;code = 73
char = );code = 41
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = e;code = 101
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = s;code = 115
char = o;code = 111
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = r;code = 114
char = i;code = 105
char = o;code = 111
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = i;code = 105
char = s;code = 115
char = s;code = 115
char = u;code = 117
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = f;code = 102
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = e;code = 101
char = n;code = 110
char = d;code = 100
char = o;code = 111
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = o;code = 111
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = g;code = 103
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = b;code = 98
char = o;code = 111
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = n;code = 110
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = n;code = 110
char = o;code = 111
char = i;code = 105
char = d;code = 100
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = c;code = 99
char = e;code = 101
char = p;code = 112
char = t;code = 116
char = o;code = 111
char = r;code = 114
char = s;code = 115
char = ,;code = 44
char = ;code = 32
char = C;code = 67
char = B;code = 66
char = 1;code = 49
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = C;code = 67
char = B;code = 66
char = 2;code = 50
char = .;code = 46
char = ;code = 32
char = H;code = 72
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = p;code = 112
char = o;code = 111
char = r;code = 114
char = t;code = 116
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = l;code = 108
char = e;code = 101
char = e;code = 101
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = b;code = 98
char = r;code = 114
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = g;code = 103
char = u;code = 117
char = t;code = 116
char = ,;code = 44
char = ;code = 32
char = 2;code = 50
char = -;code = 45
char = A;code = 65
char = r;code = 114
char = a;code = 97
char = -;code = 45
char = G;code = 71
char = I;code = 73
char = ;code = 32
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = c;code = 99
char = o;code = 111
char = m;code = 109
char = p;code = 112
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = s;code = 115
char = e;code = 101
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char = e;code = 101
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char = a;code = 97
char = l;code = 108
char = ;code = 32
char = 2;code = 50
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char = a;code = 97
char = c;code = 99
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char = l;code = 108
char = -;code = 45
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
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char = o;code = 111
char = l;code = 108
char = ;code = 32
char = e;code = 101
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char = e;code = 101
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char = s;code = 115
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = w;code = 119
char = o;code = 111
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = j;code = 106
char = o;code = 111
char = r;code = 114
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char = o;code = 111
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char = e;code = 101
char = s;code = 115
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = 2;code = 50
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char = l;code = 108
char = i;code = 105
char = n;code = 110
char = o;code = 111
char = l;code = 108
char = e;code = 101
char = o;code = 111
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char = l;code = 108
char = -;code = 45
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = (;code = 40
char = 2;code = 50
char = -;code = 45
char = L;code = 76
char = i;code = 105
char = n;code = 110
char = o;code = 111
char = -;code = 45
char = G;code = 71
char = l;code = 108
char = );code = 41
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = 2;code = 50
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char = p;code = 112
char = a;code = 97
char = l;code = 108
char = m;code = 109
char = i;code = 105
char = t;code = 116
char = o;code = 111
char = y;code = 121
char = l;code = 108
char = -;code = 45
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = (;code = 40
char = 2;code = 50
char = -;code = 45
char = P;code = 80
char = a;code = 97
char = l;code = 108
char = m;code = 109
char = -;code = 45
char = G;code = 71
char = l;code = 108
char = );code = 41
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
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char = s;code = 115
char = e;code = 101
char = ;code = 32
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char = w;code = 119
char = o;code = 111
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char = e;code = 101
char = s;code = 115
char = t;code = 116
char = e;code = 101
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char = s;code = 115
char = ;code = 32
char = d;code = 100
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char = ;code = 32
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char = o;code = 111
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char = ;code = 32
char = b;code = 98
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char = n;code = 110
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = c;code = 99
char = a;code = 97
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char = n;code = 110
char = a;code = 97
char = b;code = 98
char = i;code = 105
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char = i;code = 105
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char = c;code = 99
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char = s;code = 115
char = ,;code = 44
char = ;code = 32
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char = r;code = 114
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char = d;code = 100
char = o;code = 111
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char = i;code = 105
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char = y;code = 121
char = l;code = 108
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char = c;code = 99
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char = c;code = 99
char = l;code = 108
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char = v;code = 118
char = i;code = 105
char = a;code = 97
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char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = C;code = 67
char = B;code = 66
char = 1;code = 49
char = ;code = 32
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = C;code = 67
char = B;code = 66
char = 2;code = 50
char = ;;code = 59
char = ;code = 32
char = h;code = 104
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char = e;code = 101
char = v;code = 118
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char = r;code = 114
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char = g;code = 103
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char = f;code = 102
char = i;code = 105
char = c;code = 99
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char = l;code = 108
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char = p;code = 112
char = a;code = 97
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char = b;code = 98
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char = o;code = 111
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char = ;code = 32
char = 2;code = 50
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char = a;code = 97
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char = G;code = 71
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char = p;code = 112
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char = i;code = 105
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char = i;code = 105
char = b;code = 98
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char = l;code = 108
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char = c;code = 99
char = l;code = 108
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char = .;code = 46
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char = T;code = 84
char = o;code = 111
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char = f;code = 102
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char = a;code = 97
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char = h;code = 104
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char = u;code = 117
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char = d;code = 100
char = ;code = 32
char = b;code = 98
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char = 2;code = 50
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char = A;code = 65
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char = a;code = 97
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char = G;code = 71
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char = m;code = 109
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char = c;code = 99
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char = 2;code = 50
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char = L;code = 76
char = i;code = 105
char = n;code = 110
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result sql = 2-Arachidonoyl-glycerol (2-Ara-GI) has been isolated from various tissues and identified as an endogenous ligand for both cannabinoid receptors, CB1 and CB2. Here we report that in spleen, as in brain and gut, 2-Ara-GI is accompanied by several 2-acyl-glycerol esters, two major ones being 2-linoleoyl-glycerol (2-Lino-Gl) and 2-palmitoyl-glycerol (2-Palm-Gl). These two esters do not bind to the cannabinoid receptors, nor do they inhibit adenylyl cyclase via either CB1 or CB2; however, they significantly potentiate the apparent binding of 2-Ara-Gl and its apparent capacity to inhibit adenylyl cyclase. Together these esters also significantly potentiate 2-Ara-Gl inhibition of motor behavior, immobility on a ring, analgesia on a hot plate and hypothermia caused by 2-Ara-Gl in mice. 2-Lino-Gl, but not 2-Palm-GI, significantly inhibits the inactivation of 2-Ara-Gl by neuronal and basophilic cells. These data indicate that the biological activity of 2-Ara-Gl can be increased by related, endogenous 2-acyl-glycerols, which alone show no significant activity in any of the tests employed. This effect ('entourage effect') may represent a novel route for molecular regulation of endogenous cannabinoid activity.

Publication: Eur. J. Pharmacol. 1998 Jul 17 ;353(1)::23-31.
Publication Date: 1998 Jul 17
Study Author(s): Ben-Shabat, S;Fride, E;Sheskin, T;Tamiri, T;Rhee, M H;Vogel, Z;Bisogno, T;De Petrocellis, L;Di Marzo, V;Mechoulam, R;
Institution: Department of Natural Products, The Hebrew University Medical Faculty, Jerusalem, Israel.
Shortcut link to this study:
2-Arachidonoyl-glycerol (2-Ara-GI) has been isolated from various tissues and identified as an endogenous ligand for both cannabinoid receptors, CB1 and CB2. Here we report that in spleen, as in brain and gut, 2-Ara-GI is accompanied by several 2-acyl-glycerol esters, two major ones being 2-linoleoyl-glycerol (2-Lino-Gl) and 2-palmitoyl-glycerol (2-Palm-Gl). These two esters do not bind to the cannabinoid receptors, nor do they inhibit adenylyl cyclase via either CB1 or CB2; however, they significantly potentiate the apparent binding of 2-Ara-Gl and its apparent capacity to inhibit adenylyl cyclase. Together these esters also significantly potentiate 2-Ara-Gl inhibition of motor behavior, immobility on a ring, analgesia on a hot plate and hypothermia caused by 2-Ara-Gl in mice. 2-Lino-Gl, but not 2-Palm-GI, significantly inhibits the inactivation of 2-Ara-Gl by neuronal and basophilic cells. These data indicate that the biological activity of 2-Ara-Gl can be increased by related, endogenous 2-acyl-glycerols, which alone show no significant activity in any of the tests employed. This effect ('entourage effect') may represent a novel route for molecular regulation of endogenous cannabinoid activity.
PMID: 9721036

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