|
|
char = E;code = 69
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char = h;code = 104
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char = n;code = 110
char = c;code = 99
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char = e;code = 101
char = d;code = 100
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char = p;code = 112
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char = c;code = 99
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char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
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char = r;code = 114
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char = m;code = 109
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char = r;code = 114
char = a;code = 97
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = .;code = 46
result sql = Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol.
Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol.char = A;code = 65
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char = ;code = 32
char = a;code = 97
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char = t;code = 116
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char = i;code = 105
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char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = p;code = 112
char = y;code = 121
char = .;code = 46
char = ;code = 32
char = R;code = 82
char = e;code = 101
char = s;code = 115
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
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char = a;code = 97
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char = h;code = 104
char = a;code = 97
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char = b;code = 98
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char = i;code = 105
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char = T;code = 84
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char = e;code = 101
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char = r;code = 114
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char = c;code = 99
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char = s;code = 115
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char = n;code = 110
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char = t;code = 116
char = i;code = 105
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = c;code = 99
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char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = o;code = 111
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char = X;code = 88
char = -;code = 45
char = i;code = 105
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char = a;code = 97
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char = a;code = 97
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char = i;code = 105
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char = T;code = 84
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char = c;code = 99
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char = r;code = 114
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char = c;code = 99
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char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
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char = ;code = 32
char = e;code = 101
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char = a;code = 97
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char = d;code = 100
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char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = H;code = 72
char = E;code = 69
char = L;code = 76
char = A;code = 65
char = ;code = 32
char = (;code = 40
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = v;code = 118
char = i;code = 105
char = x;code = 120
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = r;code = 114
char = c;code = 99
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char = K;code = 75
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char = 5;code = 53
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char = 2;code = 50
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char = I;code = 73
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char = 9;code = 57
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char = s;code = 115
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char = h;code = 104
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result sql = A search for new agents that can sensitise cancer cells to ionising radiation is of continual interest and mainly due to the use of radiation in cancer therapy. Resveratrol, a powerful antioxidant has been shown to inhibit carcinogenesis in animal models. The purpose of this study was to examine whether resveratrol can sensitise cancer cells to X-irradiation. The human cancer cell lines examined were HELA (cervix carcinoma), K-562 (chronic myeloid leukemia) and IM-9 (multiple myeloma). The assays that were performed following X-irradiation (doses from 0 to 8 Gy) and/or incubation in the presence of resveratrol (concentrations ranging from 0 to 200 microM), were the following: trypan blue exclusion test to determine cell viability, cell morphology after May-Grunwald Giemsa staining, DNA profile analysis by flow cytometry to assess cell cycle distribution and the presence of the sub-G1 peak. The cell lines showed different radiation sensitivity (IM-9, high radiation sensitivity, K-562, intermediate radiation sensitivity and HELA, low radiation sensitivity) as seen by the X-irradiation dose related inhibition of cell growth and induction of apoptosis. The addition of resveratrol alone to the cell cultures induced apoptosis and inhibited cell growth from 50 (IM-9), 100 (EOL-1) or 200 microM (HELA) resveratrol concentrations. Concomitant treatment of the cells with either resveratrol and X-irradiation induced a synergical effect at the highest dose of 200 microM. These results show that resveratrol can act as a potential radiation sensitiser at high concentrations. Further studies need to address the toxicity of resveratrol on normal cells.
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char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = f;code = 102
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = e;code = 101
char = a;code = 97
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char = m;code = 109
char = e;code = 101
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char = t;code = 116
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char = w;code = 119
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char = t;code = 116
char = h;code = 104
char = ;code = 32
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char = e;code = 101
char = s;code = 115
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = .;code = 46
result sql = Enhanced radiation-induced apoptosis of cancer cell lines after treatment with resveratrol.
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = a;code = 97
char = r;code = 114
char = c;code = 99
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char = ;code = 32
char = f;code = 102
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char = r;code = 114
char = ;code = 32
char = n;code = 110
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char = w;code = 119
char = ;code = 32
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = s;code = 115
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char = n;code = 110
char = s;code = 115
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char = t;code = 116
char = i;code = 105
char = s;code = 115
char = e;code = 101
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char = n;code = 110
char = c;code = 99
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char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
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char = t;code = 116
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char = i;code = 105
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char = n;code = 110
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char = d;code = 100
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char = a;code = 97
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char = i;code = 105
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char = n;code = 110
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char = i;code = 105
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = c;code = 99
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char = e;code = 101
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char = d;code = 100
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char = m;code = 109
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char = e;code = 101
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char = o;code = 111
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char = t;code = 116
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char = ;code = 32
char = u;code = 117
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char = e;code = 101
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char = d;code = 100
char = i;code = 105
char = a;code = 97
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char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = p;code = 112
char = y;code = 121
char = .;code = 46
char = ;code = 32
char = R;code = 82
char = e;code = 101
char = s;code = 115
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = r;code = 114
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char = ;code = 32
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char = ;code = 32
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char = a;code = 97
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char = d;code = 100
char = a;code = 97
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char = t;code = 116
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char = h;code = 104
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = e;code = 101
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char = ;code = 32
char = s;code = 115
char = h;code = 104
char = o;code = 111
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char = n;code = 110
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char = t;code = 116
char = o;code = 111
char = ;code = 32
char = i;code = 105
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char = h;code = 104
char = i;code = 105
char = b;code = 98
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char = t;code = 116
char = ;code = 32
char = c;code = 99
char = a;code = 97
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char = c;code = 99
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char = e;code = 101
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char = e;code = 101
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char = s;code = 115
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char = T;code = 84
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char = e;code = 101
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char = p;code = 112
char = u;code = 117
char = r;code = 114
char = p;code = 112
char = o;code = 111
char = s;code = 115
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = i;code = 105
char = s;code = 115
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char = s;code = 115
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char = u;code = 117
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char = y;code = 121
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = a;code = 97
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = w;code = 119
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char = e;code = 101
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = ;code = 32
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = n;code = 110
char = s;code = 115
char = i;code = 105
char = t;code = 116
char = i;code = 105
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = X;code = 88
char = -;code = 45
char = i;code = 105
char = r;code = 114
char = r;code = 114
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = a;code = 97
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char = i;code = 105
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char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = h;code = 104
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char = c;code = 99
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char = c;code = 99
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char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
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char = ;code = 32
char = e;code = 101
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = H;code = 72
char = E;code = 69
char = L;code = 76
char = A;code = 65
char = ;code = 32
char = (;code = 40
char = c;code = 99
char = e;code = 101
char = r;code = 114
char = v;code = 118
char = i;code = 105
char = x;code = 120
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = r;code = 114
char = c;code = 99
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char = m;code = 109
char = a;code = 97
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char = ,;code = 44
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char = K;code = 75
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char = 5;code = 53
char = 6;code = 54
char = 2;code = 50
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char = r;code = 114
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char = i;code = 105
char = c;code = 99
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char = l;code = 108
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char = d;code = 100
char = ;code = 32
char = l;code = 108
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char = k;code = 107
char = e;code = 101
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char = a;code = 97
char = );code = 41
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = I;code = 73
char = M;code = 77
char = -;code = 45
char = 9;code = 57
char = ;code = 32
char = (;code = 40
char = m;code = 109
char = u;code = 117
char = l;code = 108
char = t;code = 116
char = i;code = 105
char = p;code = 112
char = l;code = 108
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char = a;code = 97
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char = .;code = 46
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char = T;code = 84
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char = a;code = 97
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char = s;code = 115
char = a;code = 97
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char = s;code = 115
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char = a;code = 97
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char = l;code = 108
char = o;code = 111
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char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = X;code = 88
char = -;code = 45
char = i;code = 105
char = r;code = 114
char = r;code = 114
char = a;code = 97
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char = e;code = 101
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char = m;code = 109
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char = o;code = 111
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char = 8;code = 56
char = ;code = 32
char = G;code = 71
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char = o;code = 111
char = n;code = 110
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = e;code = 101
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char = e;code = 101
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char = o;code = 111
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char = s;code = 115
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char = l;code = 108
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char = m;code = 109
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char = ;code = 32
char = t;code = 116
char = o;code = 111
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char = 2;code = 50
char = 0;code = 48
char = 0;code = 48
char = ;code = 32
char = m;code = 109
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char = c;code = 99
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char = o;code = 111
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char = );code = 41
char = ,;code = 44
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char = e;code = 101
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char = l;code = 108
char = l;code = 108
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char = i;code = 105
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char = o;code = 111
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char = e;code = 101
char = s;code = 115
char = t;code = 116
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char = t;code = 116
char = o;code = 111
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char = d;code = 100
char = e;code = 101
char = t;code = 116
char = e;code = 101
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char = m;code = 109
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char = n;code = 110
char = e;code = 101
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char = c;code = 99
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char = l;code = 108
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char = a;code = 97
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char = M;code = 77
char = a;code = 97
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char = n;code = 110
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char = a;code = 97
char = l;code = 108
char = d;code = 100
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char = G;code = 71
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char = e;code = 101
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char = s;code = 115
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char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ,;code = 44
char = ;code = 32
char = D;code = 68
char = N;code = 78
char = A;code = 65
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
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char = l;code = 108
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char = a;code = 97
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char = s;code = 115
char = e;code = 101
char = s;code = 115
char = s;code = 115
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char = l;code = 108
char = l;code = 108
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char = y;code = 121
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char = e;code = 101
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char = d;code = 100
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char = r;code = 114
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char = b;code = 98
char = u;code = 117
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = a;code = 97
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char = d;code = 100
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char = t;code = 116
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char = a;code = 97
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char = e;code = 101
char = n;code = 110
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char = ;code = 32
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char = c;code = 99
char = e;code = 101
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char = l;code = 108
char = s;code = 115
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char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = i;code = 105
char = n;code = 110
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
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char = n;code = 110
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char = c;code = 99
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char = ;code = 32
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char = e;code = 101
char = s;code = 115
char = s;code = 115
char = ;code = 32
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char = h;code = 104
char = e;code = 101
char = ;code = 32
char = t;code = 116
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char = i;code = 105
char = t;code = 116
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result sql = A search for new agents that can sensitise cancer cells to ionising radiation is of continual interest and mainly due to the use of radiation in cancer therapy. Resveratrol, a powerful antioxidant has been shown to inhibit carcinogenesis in animal models. The purpose of this study was to examine whether resveratrol can sensitise cancer cells to X-irradiation. The human cancer cell lines examined were HELA (cervix carcinoma), K-562 (chronic myeloid leukemia) and IM-9 (multiple myeloma). The assays that were performed following X-irradiation (doses from 0 to 8 Gy) and/or incubation in the presence of resveratrol (concentrations ranging from 0 to 200 microM), were the following: trypan blue exclusion test to determine cell viability, cell morphology after May-Grunwald Giemsa staining, DNA profile analysis by flow cytometry to assess cell cycle distribution and the presence of the sub-G1 peak. The cell lines showed different radiation sensitivity (IM-9, high radiation sensitivity, K-562, intermediate radiation sensitivity and HELA, low radiation sensitivity) as seen by the X-irradiation dose related inhibition of cell growth and induction of apoptosis. The addition of resveratrol alone to the cell cultures induced apoptosis and inhibited cell growth from 50 (IM-9), 100 (EOL-1) or 200 microM (HELA) resveratrol concentrations. Concomitant treatment of the cells with either resveratrol and X-irradiation induced a synergical effect at the highest dose of 200 microM. These results show that resveratrol can act as a potential radiation sensitiser at high concentrations. Further studies need to address the toxicity of resveratrol on normal cells.
Publication: Int. J. Mol. Med. 2004 Jun ;13(6)::895-902.
Publication Date: 2004 Jun
Study Author(s): Baatout, Sarah;Derradji, Hanane;Jacquet, Paul;Ooms, Daniella;Michaux, Arlette;Mergeay, Max;
Institution: Laboratory of Radiobiology, Belgian Nuclear Research Center, SCK-CEN, B-2400 Mol, Belgium. [email protected]
Shortcut link to this study:
Publication Date: 2004 Jun
Study Author(s): Baatout, Sarah;Derradji, Hanane;Jacquet, Paul;Ooms, Daniella;Michaux, Arlette;Mergeay, Max;
Institution: Laboratory of Radiobiology, Belgian Nuclear Research Center, SCK-CEN, B-2400 Mol, Belgium. [email protected]
Shortcut link to this study:
A search for new agents that can sensitise cancer cells to ionising radiation is of continual interest and mainly due to the use of radiation in cancer therapy. Resveratrol, a powerful antioxidant has been shown to inhibit carcinogenesis in animal models. The purpose of this study was to examine whether resveratrol can sensitise cancer cells to X-irradiation. The human cancer cell lines examined were HELA (cervix carcinoma), K-562 (chronic myeloid leukemia) and IM-9 (multiple myeloma). The assays that were performed following X-irradiation (doses from 0 to 8 Gy) and/or incubation in the presence of resveratrol (concentrations ranging from 0 to 200 microM), were the following: trypan blue exclusion test to determine cell viability, cell morphology after May-Grunwald Giemsa staining, DNA profile analysis by flow cytometry to assess cell cycle distribution and the presence of the sub-G1 peak. The cell lines showed different radiation sensitivity (IM-9, high radiation sensitivity, K-562, intermediate radiation sensitivity and HELA, low radiation sensitivity) as seen by the X-irradiation dose related inhibition of cell growth and induction of apoptosis. The addition of resveratrol alone to the cell cultures induced apoptosis and inhibited cell growth from 50 (IM-9), 100 (EOL-1) or 200 microM (HELA) resveratrol concentrations. Concomitant treatment of the cells with either resveratrol and X-irradiation induced a synergical effect at the highest dose of 200 microM. These results show that resveratrol can act as a potential radiation sensitiser at high concentrations. Further studies need to address the toxicity of resveratrol on normal cells.
PMID: 15138632
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