|
|
char = C;code = 67
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char = n;code = 110
char = d;code = 100
char = ;code = 32
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char = r;code = 114
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char = s;code = 115
char = s;code = 115
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char = t;code = 116
char = y;code = 121
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char = d;code = 100
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char = a;code = 97
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char = u;code = 117
char = r;code = 114
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char = s;code = 115
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char = e;code = 101
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char = m;code = 109
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char = t;code = 116
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char = v;code = 118
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char = m;code = 109
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char = e;code = 101
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char = n;code = 110
char = a;code = 97
char = t;code = 116
char = u;code = 117
char = s;code = 115
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char = L;code = 76
char = .;code = 46
char = );code = 41
char = .;code = 46
result sql = Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.).
Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.).char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = r;code = 114
char = i;code = 105
char = t;code = 116
char = i;code = 105
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char = ;code = 32
char = c;code = 99
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char = i;code = 105
char = l;code = 108
char = l;code = 108
char = i;code = 105
char = n;code = 110
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char = u;code = 117
char = r;code = 114
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = c;code = 99
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char = d;code = 100
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char = s;code = 115
char = e;code = 101
char = n;code = 110
char = s;code = 115
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char = t;code = 116
char = i;code = 105
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char = b;code = 98
char = e;code = 101
char = a;code = 97
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char = P;code = 80
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char = e;code = 101
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char = s;code = 115
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char = l;code = 108
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char = d;code = 100
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char = e;code = 101
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char = p;code = 112
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char = 8;code = 56
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char = #;code = 35
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char = 6;code = 54
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char = C;code = 67
char = .;code = 46
char = ;code = 32
char = T;code = 84
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char = e;code = 101
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char = u;code = 117
char = l;code = 108
char = l;code = 108
char = -;code = 45
char = l;code = 108
char = e;code = 101
char = n;code = 110
char = g;code = 103
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char = h;code = 104
char = ;code = 32
char = c;code = 99
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char = N;code = 78
char = A;code = 65
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char = ;code = 32
char = o;code = 111
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char = ;code = 32
char = P;code = 80
char = l;code = 108
char = S;code = 83
char = A;code = 65
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char = ,;code = 44
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char = e;code = 101
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char = e;code = 101
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char = f;code = 102
char = a;code = 97
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char = t;code = 116
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char = e;code = 101
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char = s;code = 115
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char = l;code = 108
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char = T;code = 84
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char = a;code = 97
char = m;code = 109
char = e;code = 101
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char = (;code = 40
char = O;code = 79
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char = n;code = 110
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char = o;code = 111
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char = ;code = 32
char = 6;code = 54
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char = 1;code = 49
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char = 3;code = 51
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char = a;code = 97
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char = ;code = 32
char = s;code = 115
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char = n;code = 110
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char = ;code = 32
char = 8;code = 56
char = 6;code = 54
char = %;code = 37
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char = h;code = 104
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char = o;code = 111
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char = y;code = 121
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char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = l;code = 108
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char = s;code = 115
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char = A;code = 65
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char = F;code = 70
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char = c;code = 99
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char = b;code = 98
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char = a;code = 97
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char = e;code = 101
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char = r;code = 114
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char = e;code = 101
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char = s;code = 115
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char = P;code = 80
char = l;code = 108
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char = D;code = 68
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char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = p;code = 112
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char = r;code = 114
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char = g;code = 103
char = u;code = 117
char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = s;code = 115
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char = h;code = 104
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char = e;code = 101
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char = s;code = 115
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char = t;code = 116
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char = d;code = 100
char = e;code = 101
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result sql = The critical chilling temperature of cold-sensitive lima bean (Phaseolus lunatus L.) seedlings was determined to be approx. 8°C. The full-length cDNAs of PlSAD, PlFAD2, and PlFAD3 encoding three fatty acid desaturases were isolated from lima bean leaves. The PlSAD open reading frame (ORF) had 1,317 bp and a single intron of 601 bp, encoding a polypeptide of 438 amino acids that showing 86% homology with the plastidial stearoyl-acyl carrier protein desaturase in soybean. The PlFAD2 ORF contained 1,059 bp and was uninterrupted, encoding a polypeptide of 352 amino acids having 85% identity with the microsomal omega-6 desaturase GmFAD2-3 in soybean. PlFAD3 ORF had 1,116 bp and seven introns, encoding a polypeptide of 371 amino acids showing 91% homology with the microsomal omega-3 desaturase FAD3 in cowpea. PlSAD and PlFAD2 were expressed highly in leaves but poorly in roots and stems, while PlFAD3 was expressed in three tissues. All three genes were significantly induced in leaves by drought. PlSAD and PlFAD3 in leaves were down-regulated by high temperature and salinity, whereas PlFAD2 was up-regulated by the two stresses. The expression patterns of the three genes in lima bean leaves under suboptimal temperature, 18°C, were different from those under 8°C. The down-regulation of PlSAD transcript at chilling temperature might be an important factor contributing to chilling susceptibility for lima bean plants.
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char = n;code = 110
char = d;code = 100
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = p;code = 112
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char = e;code = 101
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char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = o;code = 111
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char = ;code = 32
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char = h;code = 104
char = r;code = 114
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char = e;code = 101
char = ;code = 32
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char = t;code = 116
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char = P;code = 80
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char = a;code = 97
char = s;code = 115
char = e;code = 101
char = o;code = 111
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char = s;code = 115
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char = l;code = 108
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char = a;code = 97
char = t;code = 116
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = L;code = 76
char = .;code = 46
char = );code = 41
char = .;code = 46
result sql = Cloning and expression of three fatty acid desaturase genes from cold-sensitive lima bean (Phaseolus lunatus L.).
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char = 8;code = 56
char = &;code = 38
char = #;code = 35
char = 1;code = 49
char = 7;code = 55
char = 6;code = 54
char = ;;code = 59
char = C;code = 67
char = .;code = 46
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char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = f;code = 102
char = u;code = 117
char = l;code = 108
char = l;code = 108
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char = l;code = 108
char = e;code = 101
char = n;code = 110
char = g;code = 103
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = c;code = 99
char = D;code = 68
char = N;code = 78
char = A;code = 65
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = P;code = 80
char = l;code = 108
char = S;code = 83
char = A;code = 65
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char = ,;code = 44
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char = 2;code = 50
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char = P;code = 80
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char = F;code = 70
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char = 3;code = 51
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char = e;code = 101
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char = r;code = 114
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char = e;code = 101
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char = a;code = 97
char = t;code = 116
char = t;code = 116
char = y;code = 121
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char = d;code = 100
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char = d;code = 100
char = e;code = 101
char = s;code = 115
char = a;code = 97
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char = u;code = 117
char = r;code = 114
char = a;code = 97
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char = e;code = 101
char = s;code = 115
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char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = i;code = 105
char = s;code = 115
char = o;code = 111
char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = o;code = 111
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char = l;code = 108
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char = m;code = 109
char = a;code = 97
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char = b;code = 98
char = e;code = 101
char = a;code = 97
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char = a;code = 97
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char = e;code = 101
char = s;code = 115
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char = T;code = 84
char = h;code = 104
char = e;code = 101
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char = P;code = 80
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char = A;code = 65
char = D;code = 68
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char = o;code = 111
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char = e;code = 101
char = n;code = 110
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char = r;code = 114
char = e;code = 101
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = n;code = 110
char = g;code = 103
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char = f;code = 102
char = r;code = 114
char = a;code = 97
char = m;code = 109
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = O;code = 79
char = R;code = 82
char = F;code = 70
char = );code = 41
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = d;code = 100
char = ;code = 32
char = 1;code = 49
char = ,;code = 44
char = 3;code = 51
char = 1;code = 49
char = 7;code = 55
char = ;code = 32
char = b;code = 98
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = s;code = 115
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char = n;code = 110
char = g;code = 103
char = l;code = 108
char = e;code = 101
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char = i;code = 105
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 6;code = 54
char = 0;code = 48
char = 1;code = 49
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char = b;code = 98
char = p;code = 112
char = ,;code = 44
char = ;code = 32
char = e;code = 101
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char = c;code = 99
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = a;code = 97
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char = p;code = 112
char = o;code = 111
char = l;code = 108
char = y;code = 121
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char = e;code = 101
char = p;code = 112
char = t;code = 116
char = i;code = 105
char = d;code = 100
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 4;code = 52
char = 3;code = 51
char = 8;code = 56
char = ;code = 32
char = a;code = 97
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = o;code = 111
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char = a;code = 97
char = c;code = 99
char = i;code = 105
char = d;code = 100
char = s;code = 115
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char = t;code = 116
char = h;code = 104
char = a;code = 97
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char = ;code = 32
char = s;code = 115
char = h;code = 104
char = o;code = 111
char = w;code = 119
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char = n;code = 110
char = g;code = 103
char = ;code = 32
char = 8;code = 56
char = 6;code = 54
char = %;code = 37
char = ;code = 32
char = h;code = 104
char = o;code = 111
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char = o;code = 111
char = l;code = 108
char = o;code = 111
char = g;code = 103
char = y;code = 121
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char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = l;code = 108
char = a;code = 97
char = s;code = 115
char = t;code = 116
char = i;code = 105
char = d;code = 100
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = s;code = 115
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char = e;code = 101
char = a;code = 97
char = r;code = 114
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char = -;code = 45
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char = y;code = 121
char = l;code = 108
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char = r;code = 114
char = i;code = 105
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = i;code = 105
char = n;code = 110
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char = e;code = 101
char = s;code = 115
char = a;code = 97
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = s;code = 115
char = o;code = 111
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char = b;code = 98
char = e;code = 101
char = a;code = 97
char = n;code = 110
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = P;code = 80
char = l;code = 108
char = F;code = 70
char = A;code = 65
char = D;code = 68
char = 2;code = 50
char = ;code = 32
char = O;code = 79
char = R;code = 82
char = F;code = 70
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
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char = 1;code = 49
char = ,;code = 44
char = 0;code = 48
char = 5;code = 53
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char = b;code = 98
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = u;code = 117
char = n;code = 110
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = r;code = 114
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char = p;code = 112
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char = e;code = 101
char = d;code = 100
char = ,;code = 44
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char = d;code = 100
char = i;code = 105
char = n;code = 110
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char = a;code = 97
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char = p;code = 112
char = o;code = 111
char = l;code = 108
char = y;code = 121
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char = e;code = 101
char = p;code = 112
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char = i;code = 105
char = d;code = 100
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 3;code = 51
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char = 2;code = 50
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char = a;code = 97
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char = i;code = 105
char = n;code = 110
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char = a;code = 97
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char = i;code = 105
char = d;code = 100
char = s;code = 115
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char = h;code = 104
char = a;code = 97
char = v;code = 118
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = 8;code = 56
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char = %;code = 37
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char = d;code = 100
char = e;code = 101
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char = t;code = 116
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char = t;code = 116
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char = t;code = 116
char = h;code = 104
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char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = s;code = 115
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char = a;code = 97
char = l;code = 108
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char = o;code = 111
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char = e;code = 101
char = g;code = 103
char = a;code = 97
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char = 6;code = 54
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char = d;code = 100
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char = s;code = 115
char = a;code = 97
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = G;code = 71
char = m;code = 109
char = F;code = 70
char = A;code = 65
char = D;code = 68
char = 2;code = 50
char = -;code = 45
char = 3;code = 51
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = y;code = 121
char = b;code = 98
char = e;code = 101
char = a;code = 97
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char = .;code = 46
char = ;code = 32
char = P;code = 80
char = l;code = 108
char = F;code = 70
char = A;code = 65
char = D;code = 68
char = 3;code = 51
char = ;code = 32
char = O;code = 79
char = R;code = 82
char = F;code = 70
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = d;code = 100
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char = 1;code = 49
char = ,;code = 44
char = 1;code = 49
char = 1;code = 49
char = 6;code = 54
char = ;code = 32
char = b;code = 98
char = p;code = 112
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = s;code = 115
char = e;code = 101
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char = e;code = 101
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char = i;code = 105
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char = o;code = 111
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char = s;code = 115
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char = e;code = 101
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char = c;code = 99
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = n;code = 110
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char = ;code = 32
char = a;code = 97
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char = p;code = 112
char = o;code = 111
char = l;code = 108
char = y;code = 121
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char = e;code = 101
char = p;code = 112
char = t;code = 116
char = i;code = 105
char = d;code = 100
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 3;code = 51
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char = 1;code = 49
char = ;code = 32
char = a;code = 97
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = o;code = 111
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char = a;code = 97
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char = i;code = 105
char = d;code = 100
char = s;code = 115
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char = s;code = 115
char = h;code = 104
char = o;code = 111
char = w;code = 119
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char = n;code = 110
char = g;code = 103
char = ;code = 32
char = 9;code = 57
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char = %;code = 37
char = ;code = 32
char = h;code = 104
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char = o;code = 111
char = l;code = 108
char = o;code = 111
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char = t;code = 116
char = h;code = 104
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char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = m;code = 109
char = i;code = 105
char = c;code = 99
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char = o;code = 111
char = s;code = 115
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char = a;code = 97
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char = o;code = 111
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char = a;code = 97
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char = a;code = 97
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char = u;code = 117
char = r;code = 114
char = a;code = 97
char = s;code = 115
char = e;code = 101
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char = F;code = 70
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char = D;code = 68
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char = P;code = 80
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char = A;code = 65
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char = a;code = 97
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char = P;code = 80
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char = F;code = 70
char = A;code = 65
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char = w;code = 119
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char = a;code = 97
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char = b;code = 98
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char = t;code = 116
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char = o;code = 111
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char = l;code = 108
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char = o;code = 111
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char = a;code = 97
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char = e;code = 101
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char = ,;code = 44
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char = h;code = 104
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char = l;code = 108
char = e;code = 101
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char = P;code = 80
char = l;code = 108
char = F;code = 70
char = A;code = 65
char = D;code = 68
char = 3;code = 51
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char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = e;code = 101
char = x;code = 120
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char = r;code = 114
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char = s;code = 115
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char = e;code = 101
char = d;code = 100
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char = h;code = 104
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char = s;code = 115
char = s;code = 115
char = u;code = 117
char = e;code = 101
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char = ;code = 32
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char = l;code = 108
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char = h;code = 104
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char = e;code = 101
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char = g;code = 103
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char = r;code = 114
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char = g;code = 103
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char = f;code = 102
char = i;code = 105
char = c;code = 99
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char = t;code = 116
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char = a;code = 97
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char = b;code = 98
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char = r;code = 114
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char = u;code = 117
char = g;code = 103
char = h;code = 104
char = t;code = 116
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char = P;code = 80
char = l;code = 108
char = S;code = 83
char = A;code = 65
char = D;code = 68
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = P;code = 80
char = l;code = 108
char = F;code = 70
char = A;code = 65
char = D;code = 68
char = 3;code = 51
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char = i;code = 105
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char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = d;code = 100
char = o;code = 111
char = w;code = 119
char = n;code = 110
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result sql = The critical chilling temperature of cold-sensitive lima bean (Phaseolus lunatus L.) seedlings was determined to be approx. 8°C. The full-length cDNAs of PlSAD, PlFAD2, and PlFAD3 encoding three fatty acid desaturases were isolated from lima bean leaves. The PlSAD open reading frame (ORF) had 1,317 bp and a single intron of 601 bp, encoding a polypeptide of 438 amino acids that showing 86% homology with the plastidial stearoyl-acyl carrier protein desaturase in soybean. The PlFAD2 ORF contained 1,059 bp and was uninterrupted, encoding a polypeptide of 352 amino acids having 85% identity with the microsomal omega-6 desaturase GmFAD2-3 in soybean. PlFAD3 ORF had 1,116 bp and seven introns, encoding a polypeptide of 371 amino acids showing 91% homology with the microsomal omega-3 desaturase FAD3 in cowpea. PlSAD and PlFAD2 were expressed highly in leaves but poorly in roots and stems, while PlFAD3 was expressed in three tissues. All three genes were significantly induced in leaves by drought. PlSAD and PlFAD3 in leaves were down-regulated by high temperature and salinity, whereas PlFAD2 was up-regulated by the two stresses. The expression patterns of the three genes in lima bean leaves under suboptimal temperature, 18°C, were different from those under 8°C. The down-regulation of PlSAD transcript at chilling temperature might be an important factor contributing to chilling susceptibility for lima bean plants.
Publication: Biotechnol. Lett. 2011 Feb ;33(2)::395-401. :doi: 10.1007/s10529-010-0432-4.
Publication Date: 2011 Feb
Study Author(s): Zhang, Y M;Wang, C C;Hu, H H;Yang, L;
Institution: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004 Zhejiang, China.
Shortcut link to this study:
Publication Date: 2011 Feb
Study Author(s): Zhang, Y M;Wang, C C;Hu, H H;Yang, L;
Institution: College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, 321004 Zhejiang, China.
Shortcut link to this study:
The critical chilling temperature of cold-sensitive lima bean (Phaseolus lunatus L.) seedlings was determined to be approx. 8°C. The full-length cDNAs of PlSAD, PlFAD2, and PlFAD3 encoding three fatty acid desaturases were isolated from lima bean leaves. The PlSAD open reading frame (ORF) had 1,317 bp and a single intron of 601 bp, encoding a polypeptide of 438 amino acids that showing 86% homology with the plastidial stearoyl-acyl carrier protein desaturase in soybean. The PlFAD2 ORF contained 1,059 bp and was uninterrupted, encoding a polypeptide of 352 amino acids having 85% identity with the microsomal omega-6 desaturase GmFAD2-3 in soybean. PlFAD3 ORF had 1,116 bp and seven introns, encoding a polypeptide of 371 amino acids showing 91% homology with the microsomal omega-3 desaturase FAD3 in cowpea. PlSAD and PlFAD2 were expressed highly in leaves but poorly in roots and stems, while PlFAD3 was expressed in three tissues. All three genes were significantly induced in leaves by drought. PlSAD and PlFAD3 in leaves were down-regulated by high temperature and salinity, whereas PlFAD2 was up-regulated by the two stresses. The expression patterns of the three genes in lima bean leaves under suboptimal temperature, 18°C, were different from those under 8°C. The down-regulation of PlSAD transcript at chilling temperature might be an important factor contributing to chilling susceptibility for lima bean plants.
PMID: 20953666
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