|
|
char = R;code = 82
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char = A;code = 65
char = ;code = 32
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char = c;code = 99
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char = l;code = 108
char = s;code = 115
char = .;code = 46
result sql = Role of calcium in phototoxicity of 2-butylamino-2-demethoxy-hypocrellin A to human gastric cancer MGC-803 cells.
Role of calcium in phototoxicity of 2-butylamino-2-demethoxy-hypocrellin A to human gastric cancer MGC-803 cells.char = A;code = 65
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char = n;code = 110
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char = A;code = 65
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char = e;code = 101
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char = l;code = 108
char = c;code = 99
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char = u;code = 117
char = m;code = 109
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
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char = o;code = 111
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char = t;code = 116
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char = (;code = 40
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char = );code = 41
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char = A;code = 65
char = T;code = 84
char = P;code = 80
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char = ;code = 32
char = M;code = 77
char = e;code = 101
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char = n;code = 110
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char = i;code = 105
char = l;code = 108
char = e;code = 101
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = u;code = 117
char = c;code = 99
char = l;code = 108
char = e;code = 101
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char = s;code = 115
char = ;code = 32
char = m;code = 109
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char = o;code = 111
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char = o;code = 111
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char = ;code = 32
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char = l;code = 108
char = s;code = 115
char = o;code = 111
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result sql = After incubation with 2-butylamino-2-demethoxy-hypocrellin A (2-BA-2-DMHA), photodynamically induced change in the cytoplasmic free calcium concentration ([Ca(2+)](i)) and its effect on cell damage were investigated in human gastric cancer (MGC-803). Fluorescence spectrophotometry measurement indicated that the photosensitization of MGC-803 by 2-BA-2-DMHA caused an increase in intracellular calcium [Ca(2+)](i), and this increase in [Ca(2+)](i) showed a dependence on the concentration of 2-BA-2-DMHA, light dose and extracellular [Ca(2+)](e). This phenomenon of intracellular calcium accumulation was further confirmed by using laser scanning confocal microscopy (LSCM). Furthermore, the results from MTT assay and flow cytometry analysis suggested that chelation of extracellular calcium by EGTA or intracellular calcium by BAPTA could inhibit photodynamically induced cell killing, while increase of [Ca(2+)](i) by thapsigargin (TG), a highly specific inhibitor of the Ca(2+)-ATPase, or by A23187, a calcium ionophore could enhance this action. Meanwhile, the nucleus morphology was also investigated by fluorescence microscopy. The results indicated that the increase in intracellular Ca(2+) concentration was responsible for 2-BA-2-DMHA photodynamically induced damage to MGC-803.
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char = a;code = 97
char = l;code = 108
char = c;code = 99
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char = u;code = 117
char = m;code = 109
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = p;code = 112
char = h;code = 104
char = o;code = 111
char = t;code = 116
char = o;code = 111
char = t;code = 116
char = o;code = 111
char = x;code = 120
char = i;code = 105
char = c;code = 99
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 2;code = 50
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char = b;code = 98
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char = y;code = 121
char = l;code = 108
char = a;code = 97
char = m;code = 109
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char = 2;code = 50
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char = e;code = 101
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char = h;code = 104
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char = h;code = 104
char = y;code = 121
char = p;code = 112
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char = c;code = 99
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char = l;code = 108
char = l;code = 108
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char = A;code = 65
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = h;code = 104
char = u;code = 117
char = m;code = 109
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char = n;code = 110
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char = g;code = 103
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char = r;code = 114
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char = c;code = 99
char = a;code = 97
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = r;code = 114
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char = M;code = 77
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char = -;code = 45
char = 8;code = 56
char = 0;code = 48
char = 3;code = 51
char = ;code = 32
char = c;code = 99
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char = l;code = 108
char = l;code = 108
char = s;code = 115
char = .;code = 46
result sql = Role of calcium in phototoxicity of 2-butylamino-2-demethoxy-hypocrellin A to human gastric cancer MGC-803 cells.
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char = i;code = 105
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char = a;code = 97
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char = t;code = 116
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char = o;code = 111
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char = s;code = 115
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char = t;code = 116
char = i;code = 105
char = z;code = 122
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char = o;code = 111
char = f;code = 102
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char = b;code = 98
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char = A;code = 65
char = -;code = 45
char = 2;code = 50
char = -;code = 45
char = D;code = 68
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char = H;code = 72
char = A;code = 65
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char = c;code = 99
char = a;code = 97
char = u;code = 117
char = s;code = 115
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char = i;code = 105
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char = a;code = 97
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char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
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char = a;code = 97
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char = l;code = 108
char = u;code = 117
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char = a;code = 97
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char = C;code = 67
char = a;code = 97
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char = 2;code = 50
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char = i;code = 105
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char = s;code = 115
char = h;code = 104
char = o;code = 111
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char = d;code = 100
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char = e;code = 101
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char = ;code = 32
char = t;code = 116
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char = e;code = 101
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char = c;code = 99
char = o;code = 111
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char = c;code = 99
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char = t;code = 116
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char = a;code = 97
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char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 2;code = 50
char = -;code = 45
char = B;code = 66
char = A;code = 65
char = -;code = 45
char = 2;code = 50
char = -;code = 45
char = D;code = 68
char = M;code = 77
char = H;code = 72
char = A;code = 65
char = ,;code = 44
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char = l;code = 108
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char = g;code = 103
char = h;code = 104
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char = a;code = 97
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char = e;code = 101
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char = t;code = 116
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char = a;code = 97
char = c;code = 99
char = e;code = 101
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char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
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char = [;code = 91
char = C;code = 67
char = a;code = 97
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char = (;code = 40
char = e;code = 101
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char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = i;code = 105
char = s;code = 115
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char = a;code = 97
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char = f;code = 102
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char = .;code = 46
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char = T;code = 84
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char = E;code = 69
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char = A;code = 65
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char = B;code = 66
char = A;code = 65
char = P;code = 80
char = T;code = 84
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char = T;code = 84
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result sql = After incubation with 2-butylamino-2-demethoxy-hypocrellin A (2-BA-2-DMHA), photodynamically induced change in the cytoplasmic free calcium concentration ([Ca(2+)](i)) and its effect on cell damage were investigated in human gastric cancer (MGC-803). Fluorescence spectrophotometry measurement indicated that the photosensitization of MGC-803 by 2-BA-2-DMHA caused an increase in intracellular calcium [Ca(2+)](i), and this increase in [Ca(2+)](i) showed a dependence on the concentration of 2-BA-2-DMHA, light dose and extracellular [Ca(2+)](e). This phenomenon of intracellular calcium accumulation was further confirmed by using laser scanning confocal microscopy (LSCM). Furthermore, the results from MTT assay and flow cytometry analysis suggested that chelation of extracellular calcium by EGTA or intracellular calcium by BAPTA could inhibit photodynamically induced cell killing, while increase of [Ca(2+)](i) by thapsigargin (TG), a highly specific inhibitor of the Ca(2+)-ATPase, or by A23187, a calcium ionophore could enhance this action. Meanwhile, the nucleus morphology was also investigated by fluorescence microscopy. The results indicated that the increase in intracellular Ca(2+) concentration was responsible for 2-BA-2-DMHA photodynamically induced damage to MGC-803.
Publication: Biochim. Biophys. Acta 2003 Feb 17 ;1593(2-3)::191-200.
Publication Date: 2003 Feb 17
Study Author(s): Zhou, Zhixiang;Yang, Hongying;Zhang, Zhiyi;
Institution: Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, 100101, Beijing, PR China.
Shortcut link to this study:
Publication Date: 2003 Feb 17
Study Author(s): Zhou, Zhixiang;Yang, Hongying;Zhang, Zhiyi;
Institution: Center for Molecular Biology, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Chaoyang District, 100101, Beijing, PR China.
Shortcut link to this study:
After incubation with 2-butylamino-2-demethoxy-hypocrellin A (2-BA-2-DMHA), photodynamically induced change in the cytoplasmic free calcium concentration ([Ca(2+)](i)) and its effect on cell damage were investigated in human gastric cancer (MGC-803). Fluorescence spectrophotometry measurement indicated that the photosensitization of MGC-803 by 2-BA-2-DMHA caused an increase in intracellular calcium [Ca(2+)](i), and this increase in [Ca(2+)](i) showed a dependence on the concentration of 2-BA-2-DMHA, light dose and extracellular [Ca(2+)](e). This phenomenon of intracellular calcium accumulation was further confirmed by using laser scanning confocal microscopy (LSCM). Furthermore, the results from MTT assay and flow cytometry analysis suggested that chelation of extracellular calcium by EGTA or intracellular calcium by BAPTA could inhibit photodynamically induced cell killing, while increase of [Ca(2+)](i) by thapsigargin (TG), a highly specific inhibitor of the Ca(2+)-ATPase, or by A23187, a calcium ionophore could enhance this action. Meanwhile, the nucleus morphology was also investigated by fluorescence microscopy. The results indicated that the increase in intracellular Ca(2+) concentration was responsible for 2-BA-2-DMHA photodynamically induced damage to MGC-803.
PMID: 12581863
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