char = R;code = 82 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = v;code = 118 char = o;code = 111 char = l;code = 108 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = 2;code = 50 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = x;code = 120 char = .;code = 46 result sql = Requirement of voltage-dependent anion channel 2 for pro-apoptotic activity of Bax.
Requirement of voltage-dependent anion channel 2 for pro-apoptotic activity of Bax.char = M;code = 77 char = i;code = 105 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = B;code = 66 char = c;code = 99 char = l;code = 108 char = -;code = 45 char = 2;code = 50 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = o;code = 111 char = l;code = 108 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = i;code = 105 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = (;code = 40 char = B;code = 66 char = a;code = 97 char = x;code = 120 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = k;code = 107 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = .;code = 46 char = ;code = 32 char = W;code = 87 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = v;code = 118 char = o;code = 111 char = l;code = 108 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = (;code = 40 char = V;code = 86 char = D;code = 68 char = A;code = 65 char = C;code = 67 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = );code = 41 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = y;code = 121 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = e;code = 101 char = a;code = 97 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = B;code = 66 char = c;code = 99 char = l;code = 108 char = -;code = 45 char = 2;code = 50 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = H;code = 72 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = v;code = 118 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = d;code = 100 char = d;code = 100 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = V;code = 86 char = D;code = 68 char = A;code = 65 char = C;code = 67 char = 2;code = 50 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = x;code = 120 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = k;code = 107 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = k;code = 107 char = ,;code = 44 char = ;code = 32 char = V;code = 86 char = D;code = 68 char = A;code = 65 char = C;code = 67 char = 2;code = 50 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = -;code = 45 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = V;code = 86 char = d;code = 100 char = a;code = 97 char = c;code = 99 char = 2;code = 50 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = i;code = 105 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = l;code = 108 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = l;code = 108 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = V;code = 86 char = D;code = 68 char = A;code = 65 char = C;code = 67 char = 2;code = 50 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = k;code = 107 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = x;code = 120 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = s;code = 115 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = v;code = 118 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = i;code = 105 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = V;code = 86 char = D;code = 68 char = A;code = 65 char = C;code = 67 char = 2;code = 50 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = k;code = 107 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = x;code = 120 char = ;code = 32 char = (;code = 40 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = h;code = 104 char = o;code = 111 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = g;code = 103 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = z;code = 122 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = );code = 41 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = k;code = 107 char = e;code = 101 char = d;code = 100 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = a;code = 97 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ,;code = 44 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = V;code = 86 char = D;code = 68 char = A;code = 65 char = C;code = 67 char = 2;code = 50 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = i;code = 105 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = -;code = 45 char = a;code = 97 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = i;code = 105 char = t;code = 116 char = y;code = 121 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = x;code = 120 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = b;code = 98 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = B;code = 66 char = a;code = 97 char = k;code = 107 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Mitochondrial membrane permeabilization is central to apoptotic signaling and is directly regulated by the Bcl-2 family of proteins, consisting of anti-apoptotic members and pro-apoptotic members, although the precise mechanisms involved remain elusive. When cells are deficient in both pro-apoptotic multidomain members of this family (Bax and Bak), mitochondrial membrane permeabilization does not occur in response to various apoptotic stimuli. We have previously reported that the voltage-dependent anion channel (VDAC or porin) plays a role in apoptotic mitochondrial membrane permeabilization by interacting with Bcl-2 family members. Here, we have provided additional evidence that VDAC2 is required for pro-apoptotic activity of Bax in the absence of Bak. In the absence of Bak, VDAC2-deficient cells showed strong resistance to various apoptotic stimuli, whereas re-introduction of the Vdac2 gene restored their apoptotic response. Consistently, silencing of VDAC2 in Bak-deficient cells, but not Bax-deficient cells, also conferred resistance to various apoptotic stimuli. In the absence of VDAC2 and Bak, the activation of Bax (assessed by mitochondrial membrane integration, conformational changes and oligomerization) was markedly impaired. Taken together, these findings indicate that VDAC2 is required for pro-apoptotic activity of Bax in the absence of Bak.
Publication: Oncogene 2009 Oct 8 ;28(40)::3563-72. :doi: 10.1038/onc.2009.213.
Publication Date: 2009 Oct 8
Study Author(s): Yamagata, H;Shimizu, S;Nishida, Y;Watanabe, Y;Craigen, W J;Tsujimoto, Y;
Institution: Department of Medical Genetics, Osaka University Medical School, Suita, Osaka, Japan.
Shortcut link to this study:
Mitochondrial membrane permeabilization is central to apoptotic signaling and is directly regulated by the Bcl-2 family of proteins, consisting of anti-apoptotic members and pro-apoptotic members, although the precise mechanisms involved remain elusive. When cells are deficient in both pro-apoptotic multidomain members of this family (Bax and Bak), mitochondrial membrane permeabilization does not occur in response to various apoptotic stimuli. We have previously reported that the voltage-dependent anion channel (VDAC or porin) plays a role in apoptotic mitochondrial membrane permeabilization by interacting with Bcl-2 family members. Here, we have provided additional evidence that VDAC2 is required for pro-apoptotic activity of Bax in the absence of Bak. In the absence of Bak, VDAC2-deficient cells showed strong resistance to various apoptotic stimuli, whereas re-introduction of the Vdac2 gene restored their apoptotic response. Consistently, silencing of VDAC2 in Bak-deficient cells, but not Bax-deficient cells, also conferred resistance to various apoptotic stimuli. In the absence of VDAC2 and Bak, the activation of Bax (assessed by mitochondrial membrane integration, conformational changes and oligomerization) was markedly impaired. Taken together, these findings indicate that VDAC2 is required for pro-apoptotic activity of Bax in the absence of Bak.
PMID: 19617898