|
|
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char = m;code = 109
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char = .;code = 46
result sql = Upregulation of soluble resistance-related calcium-binding protein (sorcin) in gastric cancer.
Upregulation of soluble resistance-related calcium-binding protein (sorcin) in gastric cancer.char = T;code = 84
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result sql = The aim of this paper was to identify novel proteins involved in the development of gastric cancer (GC). Isobaric tags for relative and absolute quantification (iTRAQ) analysis was adopted to separate the differentially expressed proteins between normal gastric epithelial cell line GES-1 and GC cell line SGC7901. Western blotting was utilized to validate the increased expression of sorcin in SGC7901; immunohistochemistry was performed to investigate its relationship with clinicopathological features of GC. Twelve differential proteins were identified. Seven proteins were found to be significantly upregulated (≥two-fold), while five proteins were markedly downregulated (≤0.5-fold), in SGC7901 cells. Sorcin was detected by this proteomic approach with a 5.4-fold upregulation in SGC7901. Western blotting and immunohistochemistry confirmed the overexpression of sorcin in GC. Immunohistochemistry showed us that sorcin was overexpressed in 55 samples of GC tissue (55/85, 64.71%) and was related closely to the depth of invasion, TNM stage, and lymph node metastasis of GC (P<0.05). The development of GC is regulated by multiple genes. Sorcin will be a novel molecular biomarker for the diagnosis, treatment, and prognosis of GC.
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result sql = Upregulation of soluble resistance-related calcium-binding protein (sorcin) in gastric cancer.
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char = m;code = 109
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char = ;code = 32
char = b;code = 98
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char = o;code = 111
char = m;code = 109
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char = k;code = 107
char = e;code = 101
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char = f;code = 102
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char = ;code = 32
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char = e;code = 101
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char = i;code = 105
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char = g;code = 103
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char = s;code = 115
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char = n;code = 110
char = o;code = 111
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = G;code = 71
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result sql = The aim of this paper was to identify novel proteins involved in the development of gastric cancer (GC). Isobaric tags for relative and absolute quantification (iTRAQ) analysis was adopted to separate the differentially expressed proteins between normal gastric epithelial cell line GES-1 and GC cell line SGC7901. Western blotting was utilized to validate the increased expression of sorcin in SGC7901; immunohistochemistry was performed to investigate its relationship with clinicopathological features of GC. Twelve differential proteins were identified. Seven proteins were found to be significantly upregulated (≥two-fold), while five proteins were markedly downregulated (≤0.5-fold), in SGC7901 cells. Sorcin was detected by this proteomic approach with a 5.4-fold upregulation in SGC7901. Western blotting and immunohistochemistry confirmed the overexpression of sorcin in GC. Immunohistochemistry showed us that sorcin was overexpressed in 55 samples of GC tissue (55/85, 64.71%) and was related closely to the depth of invasion, TNM stage, and lymph node metastasis of GC (P<0.05). The development of GC is regulated by multiple genes. Sorcin will be a novel molecular biomarker for the diagnosis, treatment, and prognosis of GC.
Publication: Med. Oncol. 2010 Dec ;27(4)::1102-8. :doi: 10.1007/s12032-009-9342-5.
Publication Date: 2010 Dec
Study Author(s): Deng, Langmei;Su, Tao;Leng, Aimin;Zhang, Xiaomei;Xu, Meihua;Yan, Lu;Gu, Huan;Zhang, Guiying;
Institution: Department of Gastroenterology, Xiangya Hospital, Central South University, 87 Xiangya Road, 410008, Changsha, Hunan Province, People's Republic of China. [email protected]
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Publication Date: 2010 Dec
Study Author(s): Deng, Langmei;Su, Tao;Leng, Aimin;Zhang, Xiaomei;Xu, Meihua;Yan, Lu;Gu, Huan;Zhang, Guiying;
Institution: Department of Gastroenterology, Xiangya Hospital, Central South University, 87 Xiangya Road, 410008, Changsha, Hunan Province, People's Republic of China. [email protected]
Shortcut link to this study:
The aim of this paper was to identify novel proteins involved in the development of gastric cancer (GC). Isobaric tags for relative and absolute quantification (iTRAQ) analysis was adopted to separate the differentially expressed proteins between normal gastric epithelial cell line GES-1 and GC cell line SGC7901. Western blotting was utilized to validate the increased expression of sorcin in SGC7901; immunohistochemistry was performed to investigate its relationship with clinicopathological features of GC. Twelve differential proteins were identified. Seven proteins were found to be significantly upregulated (≥two-fold), while five proteins were markedly downregulated (≤0.5-fold), in SGC7901 cells. Sorcin was detected by this proteomic approach with a 5.4-fold upregulation in SGC7901. Western blotting and immunohistochemistry confirmed the overexpression of sorcin in GC. Immunohistochemistry showed us that sorcin was overexpressed in 55 samples of GC tissue (55/85, 64.71%) and was related closely to the depth of invasion, TNM stage, and lymph node metastasis of GC (P<0.05). The development of GC is regulated by multiple genes. Sorcin will be a novel molecular biomarker for the diagnosis, treatment, and prognosis of GC.
PMID: 19885748
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