|
|
char = L;code = 76
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char = a;code = 97
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char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = d;code = 100
char = e;code = 101
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char = i;code = 105
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char = e;code = 101
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char = a;code = 97
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char = f;code = 102
char = o;code = 111
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char = d;code = 100
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char = e;code = 101
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char = a;code = 97
char = l;code = 108
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = -;code = 45
char = t;code = 116
char = a;code = 97
char = r;code = 114
char = g;code = 103
char = e;code = 101
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char = e;code = 101
char = d;code = 100
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char = g;code = 103
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char = e;code = 101
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char = a;code = 97
char = r;code = 114
char = c;code = 99
char = h;code = 104
char = .;code = 46
result sql = Local gene delivery: arterial thrombosis model for endothelial cell-targeted thrombolytic gene therapy research.
Local gene delivery: arterial thrombosis model for endothelial cell-targeted thrombolytic gene therapy research.char = T;code = 84
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char = e;code = 101
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char = s;code = 115
char = c;code = 99
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char = b;code = 98
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char = ;code = 32
char = a;code = 97
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char = r;code = 114
char = a;code = 97
char = b;code = 98
char = b;code = 98
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char = e;code = 101
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char = a;code = 97
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char = s;code = 115
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char = t;code = 116
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char = s;code = 115
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char = a;code = 97
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char = u;code = 117
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = s;code = 115
char = u;code = 117
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
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char = e;code = 101
char = l;code = 108
char = i;code = 105
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = y;code = 121
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char = d;code = 100
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char = o;code = 111
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char = f;code = 102
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char = (;code = 40
char = C;code = 67
char = F;code = 70
char = A;code = 65
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char = .;code = 46
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char = U;code = 85
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char = ;code = 32
char = o;code = 111
char = f;code = 102
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char = t;code = 116
char = h;code = 104
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char = ;code = 32
char = t;code = 116
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char = ;code = 32
char = c;code = 99
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char = a;code = 97
char = c;code = 99
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char = e;code = 101
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char = f;code = 102
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char = u;code = 117
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char = o;code = 111
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char = c;code = 99
char = /;code = 47
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char = i;code = 105
char = t;code = 116
char = h;code = 104
char = r;code = 114
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char = m;code = 109
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char = c;code = 99
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char = e;code = 101
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char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = p;code = 112
char = y;code = 121
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char = s;code = 115
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char = >;code = 62
result sql = The authors describe a rabbit arterial thrombosis model that employs vessel division followed by microsurgical anastomosis and transluminal sutures, to reliably induce thrombus formation in the common femoral artery (CFA). Using two objective measures, "evacuation/refill" evaluation of patency and computer-aided histomorphometric analysis of the thrombus area, thrombus formation was confirmed and characterized in the model at both short- and long-term observation time-points. In addition, a gene delivery method was developed in the CFA that employs an adenoviral vector solution injected through the inferior epigastric artery (IEA). Using this method, a marker transgene (beta-galactosidase) was delivered to endothelial cells locally and without trauma. By subsequently performing beta-galactosidase staining, effective endothelial transfection was demonstrated simultaneously with endothelial viability, with preserved endothelial synthetic function in the immediate environment of the occluding thrombus. The results suggest that these two techniques can be used together in one model, to effectively introduce a foreign therapeutic transgene into endothelial cells and to evaluate the effect of the expressed protein product in a consistent in vivo thrombosis system. This combined model may be used as one of several assays of efficacy in future endothelial cell-targeted thrombolytic/antithrombotic vascular gene therapy research.
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char = ;code = 32
char = g;code = 103
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char = e;code = 101
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = l;code = 108
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = y;code = 121
char = :;code = 58
char = ;code = 32
char = a;code = 97
char = r;code = 114
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = r;code = 114
char = o;code = 111
char = m;code = 109
char = b;code = 98
char = o;code = 111
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = m;code = 109
char = o;code = 111
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char = e;code = 101
char = l;code = 108
char = ;code = 32
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char = n;code = 110
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char = o;code = 111
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char = c;code = 99
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char = l;code = 108
char = -;code = 45
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char = e;code = 101
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char = h;code = 104
char = r;code = 114
char = o;code = 111
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char = b;code = 98
char = o;code = 111
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char = y;code = 121
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char = g;code = 103
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char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = a;code = 97
char = p;code = 112
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char = s;code = 115
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char = a;code = 97
char = r;code = 114
char = c;code = 99
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char = .;code = 46
result sql = Local gene delivery: arterial thrombosis model for endothelial cell-targeted thrombolytic gene therapy research.
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char = i;code = 105
char = a;code = 97
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char = t;code = 116
char = h;code = 104
char = r;code = 114
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char = m;code = 109
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char = u;code = 117
char = s;code = 115
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char = m;code = 109
char = a;code = 97
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char = i;code = 105
char = n;code = 110
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char = e;code = 101
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char = m;code = 109
char = m;code = 109
char = o;code = 111
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char = a;code = 97
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char = a;code = 97
char = r;code = 114
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = y;code = 121
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char = (;code = 40
char = C;code = 67
char = F;code = 70
char = A;code = 65
char = );code = 41
char = .;code = 46
char = ;code = 32
char = U;code = 85
char = s;code = 115
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char = n;code = 110
char = g;code = 103
char = ;code = 32
char = t;code = 116
char = w;code = 119
char = o;code = 111
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char = o;code = 111
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char = j;code = 106
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char = o;code = 111
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char = f;code = 102
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char = l;code = 108
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char = a;code = 97
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char = u;code = 117
char = a;code = 97
char = t;code = 116
char = i;code = 105
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char = o;code = 111
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char = p;code = 112
char = a;code = 97
char = t;code = 116
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char = c;code = 99
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = c;code = 99
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char = m;code = 109
char = p;code = 112
char = u;code = 117
char = t;code = 116
char = e;code = 101
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char = a;code = 97
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char = d;code = 100
char = e;code = 101
char = d;code = 100
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char = s;code = 115
char = t;code = 116
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char = r;code = 114
char = p;code = 112
char = h;code = 104
char = o;code = 111
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char = e;code = 101
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char = a;code = 97
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char = s;code = 115
char = i;code = 105
char = s;code = 115
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = r;code = 114
char = o;code = 111
char = m;code = 109
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char = u;code = 117
char = s;code = 115
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char = a;code = 97
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char = a;code = 97
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char = ;code = 32
char = t;code = 116
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char = r;code = 114
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char = m;code = 109
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char = u;code = 117
char = s;code = 115
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char = f;code = 102
char = o;code = 111
char = r;code = 114
char = m;code = 109
char = a;code = 97
char = t;code = 116
char = i;code = 105
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char = ;code = 32
char = w;code = 119
char = a;code = 97
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char = c;code = 99
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char = i;code = 105
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char = e;code = 101
char = d;code = 100
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char = a;code = 97
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char = d;code = 100
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char = c;code = 99
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char = a;code = 97
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char = a;code = 97
char = c;code = 99
char = t;code = 116
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char = r;code = 114
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char = z;code = 122
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char = i;code = 105
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char = a;code = 97
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char = d;code = 100
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char = F;code = 70
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char = (;code = 40
char = I;code = 73
char = E;code = 69
char = A;code = 65
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char = .;code = 46
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char = U;code = 85
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char = b;code = 98
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char = a;code = 97
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char = l;code = 108
char = y;code = 121
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char = a;code = 97
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char = m;code = 109
char = a;code = 97
char = .;code = 46
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char = B;code = 66
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char = s;code = 115
char = u;code = 117
char = b;code = 98
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char = c;code = 99
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result sql = The authors describe a rabbit arterial thrombosis model that employs vessel division followed by microsurgical anastomosis and transluminal sutures, to reliably induce thrombus formation in the common femoral artery (CFA). Using two objective measures, "evacuation/refill" evaluation of patency and computer-aided histomorphometric analysis of the thrombus area, thrombus formation was confirmed and characterized in the model at both short- and long-term observation time-points. In addition, a gene delivery method was developed in the CFA that employs an adenoviral vector solution injected through the inferior epigastric artery (IEA). Using this method, a marker transgene (beta-galactosidase) was delivered to endothelial cells locally and without trauma. By subsequently performing beta-galactosidase staining, effective endothelial transfection was demonstrated simultaneously with endothelial viability, with preserved endothelial synthetic function in the immediate environment of the occluding thrombus. The results suggest that these two techniques can be used together in one model, to effectively introduce a foreign therapeutic transgene into endothelial cells and to evaluate the effect of the expressed protein product in a consistent in vivo thrombosis system. This combined model may be used as one of several assays of efficacy in future endothelial cell-targeted thrombolytic/antithrombotic vascular gene therapy research.
Publication: J Reconstr Microsurg 1999 Jan ;15(1)::73-9.
Publication Date: 1999 Jan
Study Author(s): Shenaq, S M;Kattash, M M;Weinfeld, A B;Waugh, J M;Yüksel, E;Yüksel, M;Gura, D H;
Institution: Division of Plastic Surgery, Baylor College of Medicine, Houston, Texas 77030, USA.
Shortcut link to this study:
Publication Date: 1999 Jan
Study Author(s): Shenaq, S M;Kattash, M M;Weinfeld, A B;Waugh, J M;Yüksel, E;Yüksel, M;Gura, D H;
Institution: Division of Plastic Surgery, Baylor College of Medicine, Houston, Texas 77030, USA.
Shortcut link to this study:
The authors describe a rabbit arterial thrombosis model that employs vessel division followed by microsurgical anastomosis and transluminal sutures, to reliably induce thrombus formation in the common femoral artery (CFA). Using two objective measures, "evacuation/refill" evaluation of patency and computer-aided histomorphometric analysis of the thrombus area, thrombus formation was confirmed and characterized in the model at both short- and long-term observation time-points. In addition, a gene delivery method was developed in the CFA that employs an adenoviral vector solution injected through the inferior epigastric artery (IEA). Using this method, a marker transgene (beta-galactosidase) was delivered to endothelial cells locally and without trauma. By subsequently performing beta-galactosidase staining, effective endothelial transfection was demonstrated simultaneously with endothelial viability, with preserved endothelial synthetic function in the immediate environment of the occluding thrombus. The results suggest that these two techniques can be used together in one model, to effectively introduce a foreign therapeutic transgene into endothelial cells and to evaluate the effect of the expressed protein product in a consistent in vivo thrombosis system. This combined model may be used as one of several assays of efficacy in future endothelial cell-targeted thrombolytic/antithrombotic vascular gene therapy research.
PMID: 10025534
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