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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = n;code = 110
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char = y;code = 121
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char = i;code = 105
char = d;code = 100
char = ;code = 32
char = N;code = 78
char = G;code = 71
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char = 0;code = 48
char = 8;code = 56
char = -;code = 45
char = 1;code = 49
char = 5;code = 53
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char = l;code = 108
char = s;code = 115
char = .;code = 46
result sql = Evidence for several mechanisms of volume regulation in neuroblastoma x glioma hybrid NG108-15 cells.

Evidence for several mechanisms of volume regulation in neuroblastoma x glioma hybrid NG108-15 cells.

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char = l;code = 108
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char = i;code = 105
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char = n;code = 110
char = ;code = 32
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char = o;code = 111
char = s;code = 115
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char = o;code = 111
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char = ;code = 32
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char = l;code = 108
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char = r;code = 114
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char = 3;code = 51
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char = p;code = 112
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char = ,;code = 44
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char = i;code = 105
char = s;code = 115
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char = a;code = 97
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char = t;code = 116
char = i;code = 105
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char = 2;code = 50
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char = s;code = 115
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char = r;code = 114
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char = a;code = 97
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char = k;code = 107
char = a;code = 97
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char = a;code = 97
char = c;code = 99
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char = d;code = 100
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char = W;code = 87
char = h;code = 104
char = e;code = 101
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char = t;code = 116
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char = e;code = 101
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char = t;code = 116
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char = i;code = 105
char = c;code = 99
char = i;code = 105
char = t;code = 116
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char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = r;code = 114
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char = d;code = 100
char = u;code = 117
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char = e;code = 101
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char = a;code = 97
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char = c;code = 99
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char = s;code = 115
char = t;code = 116
char = a;code = 97
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char = t;code = 116
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char = C;code = 67
char = l;code = 108
char = -;code = 45
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = t;code = 116
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char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = b;code = 98
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char = r;code = 114
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char = p;code = 112
char = l;code = 108
char = a;code = 97
char = c;code = 99
char = i;code = 105
char = n;code = 110
char = g;code = 103
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char = N;code = 78
char = a;code = 97
char = C;code = 67
char = l;code = 108
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
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char = C;code = 67
char = a;code = 97
char = C;code = 67
char = l;code = 108
char = 2;code = 50
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char = o;code = 111
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char = M;code = 77
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char = 2;code = 50
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char = ;code = 32
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char = v;code = 118
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char = a;code = 97
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char = l;code = 108
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char = a;code = 97
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char = a;code = 97
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char = T;code = 84
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char = s;code = 115
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char = u;code = 117
char = l;code = 108
char = a;code = 97
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char = o;code = 111
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char = d;code = 100
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char = a;code = 97
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char = a;code = 97
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char = t;code = 116
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char = m;code = 109
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char = e;code = 101
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char = t;code = 116
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char = a;code = 97
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char = N;code = 78
char = G;code = 71
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char = 1;code = 49
char = 0;code = 48
char = 8;code = 56
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char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = C;code = 67
char = a;code = 97
char = 2;code = 50
char = +;code = 43
char = ,;code = 44
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = N;code = 78
char = a;code = 97
char = C;code = 67
char = l;code = 108
char = ,;code = 44
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = v;code = 118
char = o;code = 111
char = l;code = 108
char = u;code = 117
char = m;code = 109
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = r;code = 114
char = e;code = 101
char = n;code = 110
char = g;code = 103
char = t;code = 116
char = h;code = 104
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = e;code = 101
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = n;code = 110
char = a;code = 97
char = t;code = 116
char = u;code = 117
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = s;code = 115
char = y;code = 121
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = m;code = 109
char = s;code = 115
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = p;code = 112
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = i;code = 105
char = b;code = 98
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = v;code = 118
char = o;code = 111
char = l;code = 108
char = u;code = 117
char = m;code = 109
char = e;code = 101
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = g;code = 103
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = m;code = 109
char = e;code = 101
char = c;code = 99
char = h;code = 104
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = s;code = 115
char = m;code = 109
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = d;code = 100
char = i;code = 105
char = s;code = 115
char = c;code = 99
char = u;code = 117
char = s;code = 115
char = s;code = 115
char = e;code = 101
char = d;code = 100
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
result sql = Volumes of neuroblastoma x glioma hybrid NG 108-15 cells were electronically measured in order to characterize the mechanisms involved in volume regulation in isosmotic and anisosmotic conditions. The cells behave as perfect osmometers when tonicity was changed at constant chloride concentration by adding sucrose or replacing NaCl with CaCl2 or MgCl2. In contrast, the cell volume was poorly dependent on tonicity when the Cl- concentration was changed by adding NaCl or H2O. Cell shrinkage was induced by cell stirring or after a hypotonicity-induced swelling. These volume decreases were abolished by caffeine but not by ryanodine or EGTA. Shrinkage was also induced by the Ca2+ ionophore ionomycin. The ionomycin-induced volume decrease was abolished by EGTA. Cell swelling induced an outwardly rectifying Cl- current which was blocked by 5-nitro-2-(3-phenylpropylamino)benzoic acid, 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid and dihydroindenyloxy-alkanoic acid. When the tonicity was reduced at constant Cl- concentration by replacing NaCl with CaCl2 or MgCl2, the volume increased and then slowly decreased towards its control value. This regulatory volume decrease was blocked by 5-nitro-2-(3-phenylpropylamino)benzoic acid, 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid and dihydroindenyl-oxy-alkanoic acid. Long-term (hours-days) cell shrinkage was induced by a reduction of the culture medium osmolarity. Long-term cell swelling was induced by an increase of the culture medium osmolarity. These volume changes were abolished by the protein translation inhibitor cycloheximide. The results suggest that NG 108-15 cell volume is regulated by at least four interacting mechanisms controlled, respectively, by intracellular Ca2+, extracellular NaCl, cell volume and intracellular ionic strength. The speculative nature of ionic systems responsible for these volume regulating mechanisms is discussed.

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