char = M;code = 77
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char = d;code = 100
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char = l;code = 108
char = a;code = 97
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char = i;code = 105
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char = s;code = 115
char = t;code = 116
char = u;code = 117
char = d;code = 100
char = y;code = 121
char = .;code = 46
result sql = Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study.
Measurement of total fructan in foods by enzymatic/spectrophotometric method: collaborative study.
char = A;code = 65
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char = A;code = 65
char = O;code = 79
char = A;code = 65
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char = c;code = 99
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char = l;code = 108
char = a;code = 97
char = b;code = 98
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char = a;code = 97
char = t;code = 116
char = i;code = 105
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char = u;code = 117
char = d;code = 100
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char = u;code = 117
char = c;code = 99
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char = d;code = 100
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char = e;code = 101
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char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
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char = c;code = 99
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char = c;code = 99
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char = l;code = 108
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char = s;code = 115
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char = g;code = 103
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char = f;code = 102
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char = u;code = 117
char = c;code = 99
char = t;code = 116
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = f;code = 102
char = r;code = 114
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = a;code = 97
char = n;code = 110
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char = p;code = 112
char = o;code = 111
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char = y;code = 121
char = s;code = 115
char = a;code = 97
char = c;code = 99
char = c;code = 99
char = h;code = 104
char = a;code = 97
char = r;code = 114
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char = (;code = 40
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char = s;code = 115
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char = ;code = 32
char = f;code = 102
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char = d;code = 100
char = u;code = 117
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char = s;code = 115
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = a;code = 97
char = m;code = 109
char = p;code = 112
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char = i;code = 105
char = s;code = 115
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char = t;code = 116
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char = t;code = 116
char = r;code = 114
char = e;code = 101
char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = ;code = 32
char = w;code = 119
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char = t;code = 116
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char = a;code = 97
char = ;code = 32
char = m;code = 109
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char = f;code = 102
char = i;code = 105
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char = s;code = 115
char = u;code = 117
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = s;code = 115
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char = e;code = 101
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char = ,;code = 44
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char = l;code = 108
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char = a;code = 97
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char = e;code = 101
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char = c;code = 99
char = r;code = 114
char = o;code = 111
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = g;code = 103
char = l;code = 108
char = u;code = 117
char = c;code = 99
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char = e;code = 101
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char = c;code = 99
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result sql = An AOAC collaborative study was conducted to evaluate the accuracy and reliability of an enzyme assay kit procedure for measuring oligofructans and fructan polysaccharide (inulins) in mixed materials and food products. The sample is extracted with hot water, and an aliquot is treated with a mixture of sucrase (a specific sucrose-degrading enzyme), alpha-amylase, pullulanase, and maltase to hydrolyze sucrose to glucose and fructose, and starch to glucose. These reducing sugars are then reduced to sugar alcohols by treatment with alkaline borohydride solution. The solution is neutralized, and excess borohydride is removed with dilute acetic acid. The fructan is hydrolyzed to fructose and glucose using a mixture of purified exo- and endo-inulinanases (fructanase mixture). The reducing sugars produced (fructose and glucose) are measured with a spectrophotometer after reaction with para-hydroxybenzoic acid hydrazide. The samples analyzed included pure fructan, chocolate, low-fat spread, milk powder, vitamin tablets, onion powder, Jerusalem artichoke flour, wheat stalks, and a sucrose/cellulose control flour. Repeatability relative standard deviations ranged from 2.3 to 7.3%; reproducibility relative standard deviations ranged from 5.0 to 10.8%.

Publication: J AOAC Int 2000 Mar-Apr ;83(2)::356-64.
Publication Date: 2000 Mar-Apr
Study Author(s): McCleary, B V;Murphy, A;Mugford, D C;
Institution: Megazyme International Ireland Ltd., Bray Business Park, County Wicklow, Ireland.
Shortcut link to this study:
An AOAC collaborative study was conducted to evaluate the accuracy and reliability of an enzyme assay kit procedure for measuring oligofructans and fructan polysaccharide (inulins) in mixed materials and food products. The sample is extracted with hot water, and an aliquot is treated with a mixture of sucrase (a specific sucrose-degrading enzyme), alpha-amylase, pullulanase, and maltase to hydrolyze sucrose to glucose and fructose, and starch to glucose. These reducing sugars are then reduced to sugar alcohols by treatment with alkaline borohydride solution. The solution is neutralized, and excess borohydride is removed with dilute acetic acid. The fructan is hydrolyzed to fructose and glucose using a mixture of purified exo- and endo-inulinanases (fructanase mixture). The reducing sugars produced (fructose and glucose) are measured with a spectrophotometer after reaction with para-hydroxybenzoic acid hydrazide. The samples analyzed included pure fructan, chocolate, low-fat spread, milk powder, vitamin tablets, onion powder, Jerusalem artichoke flour, wheat stalks, and a sucrose/cellulose control flour. Repeatability relative standard deviations ranged from 2.3 to 7.3%; reproducibility relative standard deviations ranged from 5.0 to 10.8%.
PMID: 10772173

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