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result sql = Effects of selected herbicides on cytokine production in vitro.
Effects of selected herbicides on cytokine production in vitro.
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char = p;code = 112
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char = n;code = 110
char = d;code = 100
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char = o;code = 111
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char = ;code = 32
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = r;code = 114
char = e;code = 101
char = e;code = 101
char = ;code = 32
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char = t;code = 116
char = o;code = 111
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char = i;code = 105
char = n;code = 110
char = e;code = 101
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char = ;code = 32
char = t;code = 116
char = e;code = 101
char = s;code = 115
char = t;code = 116
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char = d;code = 100
char = ;code = 32
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char = ;code = 32
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = r;code = 114
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char = n;code = 110
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char = ;code = 32
char = M;code = 77
char = C;code = 67
char = P;code = 80
char = A;code = 65
char = ;code = 32
char = :;code = 58
char = ;code = 32
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char = b;code = 98
char = >;code = 62
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char = 0;code = 48
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char = d;code = 100
char = ;code = 32
char = 0;code = 48
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char = ;code = 32
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char = c;code = 99
char = r;code = 114
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char = M;code = 77
char = );code = 41
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = i;code = 105
char = m;code = 109
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
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char = p;code = 112
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char = h;code = 104
char = u;code = 117
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char = ;code = 32
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char = ;code = 32
char = t;code = 116
char = o;code = 111
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char = r;code = 114
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char = i;code = 105
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char = n;code = 110
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char = s;code = 115
char = m;code = 109
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char = l;code = 108
char = e;code = 101
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char = e;code = 101
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char = s;code = 115
char = ;code = 32
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char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
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char = m;code = 109
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char = g;code = 103
char = e;code = 101
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char = ;code = 32
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char = e;code = 101
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char = ;code = 32
char = c;code = 99
char = y;code = 121
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char = o;code = 111
char = k;code = 107
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = o;code = 111
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result sql = To evaluate possible deleterious effects of commonly used herbicides on leukocytes, cytokine production was selected as a sensitive indicator. After in vitro exposure of human peripheral blood mononuclear cells from normal donors, the production of all 3 cytokines tested--interferon-gamma (a type 1 cytokine), interleukin-5 (a type 2 cytokine) and tumor necrosis factor-alpha (an inflammatory cytokine)--was impaired by up to 70, 50 and 70% respectively in a concentration-dependent manner in cultures exposed to atrazine (0.03-3 microM in 1% dimethylsulfoxide, DMSO). The effect paralleled that seen with dexamethasone, a known immunosuppressive agent. Other pesticides also dissolved in DMSO--mecoprop, simazine or MCPA (each up to 1 microM)--or dissolved in phosphate-buffered saline--diuron (up to 1 microM), isoproturon (up to 3 microM), metoxuron (up to 8 microM) or metamitron (up to 80 microM)--showed no concentration-related effects on cytokine production. There was, however, an inhibition of IFN-gamma and TNF-alpha production by simazine, metoxuron and mecoprop and of all three cytokines tested by diuron MCPA : (0.01 and 0.1 microM) stimulated the production of TNF-alpha. Thus, exposure to herbicides leading to plasma levels in the micromolar range induces imbalance in cytokine production.

Publication: Life Sci. 2000 May 19 ;66(26)::2519-25.
Publication Date: 2000 May 19
Study Author(s): Hooghe, R J;Devos, S;Hooghe-Peters, E L;
Institution: Environmental Toxicology, Flemish Institute for Technological Research (VITO), Mol, Belgium. [email protected]
Shortcut link to this study:
To evaluate possible deleterious effects of commonly used herbicides on leukocytes, cytokine production was selected as a sensitive indicator. After in vitro exposure of human peripheral blood mononuclear cells from normal donors, the production of all 3 cytokines tested--interferon-gamma (a type 1 cytokine), interleukin-5 (a type 2 cytokine) and tumor necrosis factor-alpha (an inflammatory cytokine)--was impaired by up to 70, 50 and 70% respectively in a concentration-dependent manner in cultures exposed to atrazine (0.03-3 microM in 1% dimethylsulfoxide, DMSO). The effect paralleled that seen with dexamethasone, a known immunosuppressive agent. Other pesticides also dissolved in DMSO--mecoprop, simazine or MCPA (each up to 1 microM)--or dissolved in phosphate-buffered saline--diuron (up to 1 microM), isoproturon (up to 3 microM), metoxuron (up to 8 microM) or metamitron (up to 80 microM)--showed no concentration-related effects on cytokine production. There was, however, an inhibition of IFN-gamma and TNF-alpha production by simazine, metoxuron and mecoprop and of all three cytokines tested by diuron MCPA : (0.01 and 0.1 microM) stimulated the production of TNF-alpha. Thus, exposure to herbicides leading to plasma levels in the micromolar range induces imbalance in cytokine production.
PMID: 10883730

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