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char = u;code = 117
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char = r;code = 114
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char = m;code = 109
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char = a;code = 97
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char = s;code = 115
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char = x;code = 120
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char = b;code = 98
char = l;code = 108
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char = u;code = 117
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char = t;code = 116
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char = ;code = 32
char = c;code = 99
char = h;code = 104
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = e;code = 101
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char = e;code = 101
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char = ;code = 32
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char = e;code = 101
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char = e;code = 101
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char = f;code = 102
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char = i;code = 105
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char = e;code = 101
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char = s;code = 115
char = t;code = 116
char = r;code = 114
char = a;code = 97
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char = t;code = 116
char = o;code = 111
char = ;code = 32
char = d;code = 100
char = e;code = 101
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char = e;code = 101
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char = ;code = 32
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char = c;code = 99
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char = n;code = 110
char = e;code = 101
char = ;code = 32
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char = a;code = 97
char = i;code = 105
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char = t;code = 116
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char = n;code = 110
char = ;code = 32
char = i;code = 105
char = n;code = 110
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char = >;code = 62
result sql = Outbreaks of haemorrhagic fever caused by the Ebola virus are associated with high mortality rates that are a distinguishing feature of this human pathogen. The highest lethality is associated with the Zaire subtype, one of four strains identified to date. Its rapid progression allows little opportunity to develop natural immunity, and there is currently no effective anti-viral therapy. Therefore, vaccination offers a promising intervention to prevent infection and limit spread. Here we describe a highly effective vaccine strategy for Ebola virus infection in non-human primates. A combination of DNA immunization and boosting with adenoviral vectors that encode viral proteins generated cellular and humoral immunity in cynomolgus macaques. Challenge with a lethal dose of the highly pathogenic, wild-type, 1976 Mayinga strain of Ebola Zaire virus resulted in uniform infection in controls, who progressed to a moribund state and death in less than one week. In contrast, all vaccinated animals were asymptomatic for more than six months, with no detectable virus after the initial challenge. These findings demonstrate that it is possible to develop a preventive vaccine against Ebola virus infection in primates.
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char = p;code = 112
char = m;code = 109
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = c;code = 99
char = c;code = 99
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char = n;code = 110
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char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = E;code = 69
char = b;code = 98
char = o;code = 111
char = l;code = 108
char = a;code = 97
char = ;code = 32
char = v;code = 118
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char = u;code = 117
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char = i;code = 105
char = n;code = 110
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char = e;code = 101
char = c;code = 99
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char = e;code = 101
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char = .;code = 46
result sql = Development of a preventive vaccine for Ebola virus infection in primates.
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char = n;code = 110
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char = c;code = 99
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char = ;code = 32
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char = e;code = 101
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char = d;code = 100
char = ;code = 32
char = d;code = 100
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char = h;code = 104
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = l;code = 108
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char = s;code = 115
char = s;code = 115
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char = n;code = 110
char = ;code = 32
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char = n;code = 110
char = e;code = 101
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char = e;code = 101
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char = k;code = 107
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char = n;code = 110
char = ;code = 32
char = c;code = 99
char = o;code = 111
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char = t;code = 116
char = r;code = 114
char = a;code = 97
char = s;code = 115
char = t;code = 116
char = ,;code = 44
char = ;code = 32
char = a;code = 97
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char = l;code = 108
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = c;code = 99
char = c;code = 99
char = i;code = 105
char = n;code = 110
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = y;code = 121
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char = p;code = 112
char = t;code = 116
char = o;code = 111
char = m;code = 109
char = a;code = 97
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char = i;code = 105
char = c;code = 99
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char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = m;code = 109
char = o;code = 111
char = r;code = 114
char = e;code = 101
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char = t;code = 116
char = h;code = 104
char = a;code = 97
char = n;code = 110
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char = x;code = 120
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char = o;code = 111
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char = s;code = 115
char = i;code = 105
char = b;code = 98
char = l;code = 108
char = e;code = 101
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char = o;code = 111
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = o;code = 111
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char = ;code = 32
char = a;code = 97
char = ;code = 32
char = p;code = 112
char = r;code = 114
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char = v;code = 118
char = e;code = 101
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char = i;code = 105
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char = e;code = 101
char = ;code = 32
char = v;code = 118
char = a;code = 97
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char = n;code = 110
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char = ;code = 32
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char = a;code = 97
char = i;code = 105
char = n;code = 110
char = s;code = 115
char = t;code = 116
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char = o;code = 111
char = l;code = 108
char = a;code = 97
char = ;code = 32
char = v;code = 118
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char = r;code = 114
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = i;code = 105
char = m;code = 109
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char = t;code = 116
char = e;code = 101
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result sql = Outbreaks of haemorrhagic fever caused by the Ebola virus are associated with high mortality rates that are a distinguishing feature of this human pathogen. The highest lethality is associated with the Zaire subtype, one of four strains identified to date. Its rapid progression allows little opportunity to develop natural immunity, and there is currently no effective anti-viral therapy. Therefore, vaccination offers a promising intervention to prevent infection and limit spread. Here we describe a highly effective vaccine strategy for Ebola virus infection in non-human primates. A combination of DNA immunization and boosting with adenoviral vectors that encode viral proteins generated cellular and humoral immunity in cynomolgus macaques. Challenge with a lethal dose of the highly pathogenic, wild-type, 1976 Mayinga strain of Ebola Zaire virus resulted in uniform infection in controls, who progressed to a moribund state and death in less than one week. In contrast, all vaccinated animals were asymptomatic for more than six months, with no detectable virus after the initial challenge. These findings demonstrate that it is possible to develop a preventive vaccine against Ebola virus infection in primates.
Publication: Nature 2000 Nov 30 ;408(6812)::605-9.
Publication Date: 2000 Nov 30
Study Author(s): Sullivan, N J;Sanchez, A;Rollin, P E;Yang, Z Y;Nabel, G J;
Institution: Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA.
Shortcut link to this study:
Publication Date: 2000 Nov 30
Study Author(s): Sullivan, N J;Sanchez, A;Rollin, P E;Yang, Z Y;Nabel, G J;
Institution: Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA.
Shortcut link to this study:
Outbreaks of haemorrhagic fever caused by the Ebola virus are associated with high mortality rates that are a distinguishing feature of this human pathogen. The highest lethality is associated with the Zaire subtype, one of four strains identified to date. Its rapid progression allows little opportunity to develop natural immunity, and there is currently no effective anti-viral therapy. Therefore, vaccination offers a promising intervention to prevent infection and limit spread. Here we describe a highly effective vaccine strategy for Ebola virus infection in non-human primates. A combination of DNA immunization and boosting with adenoviral vectors that encode viral proteins generated cellular and humoral immunity in cynomolgus macaques. Challenge with a lethal dose of the highly pathogenic, wild-type, 1976 Mayinga strain of Ebola Zaire virus resulted in uniform infection in controls, who progressed to a moribund state and death in less than one week. In contrast, all vaccinated animals were asymptomatic for more than six months, with no detectable virus after the initial challenge. These findings demonstrate that it is possible to develop a preventive vaccine against Ebola virus infection in primates.
PMID: 11117750
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