char = M;code = 77
char = o;code = 111
char = d;code = 100
char = e;code = 101
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char = l;code = 108
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char = ;code = 32
char = b;code = 98
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = o;code = 111
char = i;code = 105
char = l;code = 108
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = l;code = 108
char = d;code = 100
char = -;code = 45
char = f;code = 102
char = i;code = 105
char = l;code = 108
char = l;code = 108
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = a;code = 97
char = d;code = 100
char = y;code = 121
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = d;code = 100
char = r;code = 114
char = i;code = 105
char = n;code = 110
char = k;code = 107
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = A;code = 65
char = c;code = 99
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = t;code = 116
char = o;code = 111
char = b;code = 98
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = c;code = 99
char = o;code = 111
char = a;code = 97
char = c;code = 99
char = e;code = 101
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = G;code = 71
char = l;code = 108
char = u;code = 117
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = o;code = 111
char = b;code = 98
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = ;code = 32
char = o;code = 111
char = x;code = 120
char = y;code = 121
char = d;code = 100
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = .;code = 46
result sql = Modelling bacterial spoilage in cold-filled ready to drink beverages by Acinetobacter calcoaceticus and Gluconobacter oxydans.
Modelling bacterial spoilage in cold-filled ready to drink beverages by Acinetobacter calcoaceticus and Gluconobacter oxydans.
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = A;code = 65
char = I;code = 73
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char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = M;code = 77
char = a;code = 97
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char = h;code = 104
char = e;code = 101
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char = a;code = 97
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = m;code = 109
char = o;code = 111
char = d;code = 100
char = e;code = 101
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
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char = e;code = 101
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char = c;code = 99
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char = e;code = 101
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = i;code = 105
char = c;code = 99
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char = ;code = 32
char = p;code = 112
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char = e;code = 101
char = d;code = 100
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char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = g;code = 103
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char = t;code = 116
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char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = s;code = 115
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char = o;code = 111
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char = l;code = 108
char = a;code = 97
char = g;code = 103
char = e;code = 101
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char = a;code = 97
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char = t;code = 116
char = e;code = 101
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char = a;code = 97
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char = i;code = 105
char = n;code = 110
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char = e;code = 101
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char = p;code = 112
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = r;code = 114
char = i;code = 105
char = o;code = 111
char = u;code = 117
char = s;code = 115
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = e;code = 101
char = r;code = 114
char = v;code = 118
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = s;code = 115
char = y;code = 121
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = m;code = 109
char = s;code = 115
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = M;code = 77
char = E;code = 69
char = T;code = 84
char = H;code = 72
char = O;code = 79
char = D;code = 68
char = S;code = 83
char = ;code = 32
char = A;code = 65
char = N;code = 78
char = D;code = 68
char = ;code = 32
char = R;code = 82
char = E;code = 69
char = S;code = 83
char = U;code = 85
char = L;code = 76
char = T;code = 84
char = S;code = 83
char = ;code = 32
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = A;code = 65
char = ;code = 32
char = B;code = 66
char = o;code = 111
char = x;code = 120
char = -;code = 45
char = B;code = 66
char = e;code = 101
char = h;code = 104
char = n;code = 110
char = k;code = 107
char = e;code = 101
char = n;code = 110
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = s;code = 115
char = i;code = 105
char = g;code = 103
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = c;code = 99
char = l;code = 108
char = u;code = 117
char = d;code = 100
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = f;code = 102
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = :;code = 58
char = ;code = 32
char = p;code = 112
char = H;code = 72
char = ;code = 32
char = (;code = 40
char = 2;code = 50
char = .;code = 46
char = 8;code = 56
char = ,;code = 44
char = ;code = 32
char = 3;code = 51
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char = 3;code = 51
char = ,;code = 44
char = ;code = 32
char = 3;code = 51
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char = ,;code = 44
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char = a;code = 97
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char = d;code = 100
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char = t;code = 116
char = y;code = 121
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char = 0;code = 48
char = .;code = 46
char = 2;code = 50
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char = %;code = 37
char = ,;code = 44
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 4;code = 52
char = 0;code = 48
char = %;code = 37
char = ,;code = 44
char = ;code = 32
char = 0;code = 48
char = .;code = 46
char = 6;code = 54
char = 0;code = 48
char = %;code = 37
char = );code = 41
char = ,;code = 44
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char = s;code = 115
char = u;code = 117
char = g;code = 103
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = (;code = 40
char = 8;code = 56
char = .;code = 46
char = 0;code = 48
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char = 1;code = 49
char = 2;code = 50
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char = ,;code = 44
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char = 1;code = 49
char = 6;code = 54
char = .;code = 46
char = 0;code = 48
char = ;code = 32
char = *;code = 42
char = ;code = 32
char = B;code = 66
char = r;code = 114
char = i;code = 105
char = x;code = 120
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
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char = u;code = 117
char = m;code = 109
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char = b;code = 98
char = e;code = 101
char = n;code = 110
char = z;code = 122
char = o;code = 111
char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = c;code = 99
char = o;code = 111
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char = c;code = 99
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char = i;code = 105
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char = n;code = 110
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char = (;code = 40
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char = 0;code = 48
char = 0;code = 48
char = ,;code = 44
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char = 2;code = 50
char = 2;code = 50
char = 5;code = 53
char = ,;code = 44
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char = 3;code = 51
char = 5;code = 53
char = 0;code = 48
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char = p;code = 112
char = p;code = 112
char = m;code = 109
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = p;code = 112
char = o;code = 111
char = t;code = 116
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = u;code = 117
char = m;code = 109
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char = s;code = 115
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char = b;code = 98
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = (;code = 40
char = 1;code = 49
char = 0;code = 48
char = 0;code = 48
char = ,;code = 44
char = ;code = 32
char = 2;code = 50
char = 2;code = 50
char = 5;code = 53
char = ,;code = 44
char = ;code = 32
char = 3;code = 51
char = 5;code = 53
char = 0;code = 48
char = ;code = 32
char = p;code = 112
char = p;code = 112
char = m;code = 109
char = );code = 41
char = .;code = 46
char = ;code = 32
char = D;code = 68
char = u;code = 117
char = p;code = 112
char = l;code = 108
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char = c;code = 99
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char = t;code = 116
char = e;code = 101
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char = s;code = 115
char = a;code = 97
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char = p;code = 112
char = l;code = 108
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char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = i;code = 105
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char = o;code = 111
char = c;code = 99
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = a;code = 97
char = ;code = 32
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char = a;code = 97
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char = a;code = 97
char = l;code = 108
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char = 1;code = 49
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char = c;code = 99
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char = ;code = 32
char = 5;code = 53
char = 0;code = 48
char = ;code = 32
char = m;code = 109
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char = );code = 41
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char = f;code = 102
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char = A;code = 65
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char = G;code = 71
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char = o;code = 111
char = b;code = 98
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char = x;code = 120
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char = .;code = 46
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char = a;code = 97
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char = e;code = 101
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char = a;code = 97
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char = m;code = 109
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char = e;code = 101
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char = o;code = 111
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char = o;code = 111
char = ,;code = 44
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char = f;code = 102
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char = ,;code = 44
char = ;code = 32
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char = d;code = 100
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char = g;code = 103
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char = <;code = 60
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char = <;code = 60
char = b;code = 98
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char = C;code = 67
char = O;code = 79
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char = C;code = 67
char = L;code = 76
char = U;code = 85
char = S;code = 83
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char = O;code = 79
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char = ;code = 32
char = :;code = 58
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char = b;code = 98
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = c;code = 99
char = i;code = 105
char = d;code = 100
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ,;code = 44
char = ;code = 32
char = s;code = 115
char = u;code = 117
char = g;code = 103
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ,;code = 44
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = d;code = 100
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = n;code = 110
char = z;code = 122
char = o;code = 111
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = p;code = 112
char = o;code = 111
char = t;code = 116
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = s;code = 115
char = o;code = 111
char = r;code = 114
char = b;code = 98
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = l;code = 108
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = s;code = 115
char = i;code = 105
char = g;code = 103
char = n;code = 110
char = i;code = 105
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = f;code = 102
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = o;code = 111
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = d;code = 100
char = i;code = 105
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = g;code = 103
char = r;code = 114
char = o;code = 111
char = w;code = 119
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = o;code = 111
char = i;code = 105
char = l;code = 108
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = b;code = 98
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
char = <;code = 60
char = b;code = 98
char = >;code = 62
char = S;code = 83
char = I;code = 73
char = G;code = 71
char = N;code = 78
char = I;code = 73
char = F;code = 70
char = I;code = 73
char = C;code = 67
char = A;code = 65
char = N;code = 78
char = C;code = 67
char = E;code = 69
char = ;code = 32
char = A;code = 65
char = N;code = 78
char = D;code = 68
char = ;code = 32
char = I;code = 73
char = M;code = 77
char = P;code = 80
char = A;code = 65
char = C;code = 67
char = T;code = 84
char = ;code = 32
char = O;code = 79
char = F;code = 70
char = ;code = 32
char = T;code = 84
char = H;code = 72
char = E;code = 69
char = ;code = 32
char = S;code = 83
char = T;code = 84
char = U;code = 85
char = D;code = 68
char = Y;code = 89
char = ;code = 32
char = :;code = 58
char = ;code = 32
char = <;code = 60
char = /;code = 47
char = b;code = 98
char = >;code = 62
char = T;code = 84
char = h;code = 104
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = o;code = 111
char = i;code = 105
char = l;code = 108
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = o;code = 111
char = d;code = 100
char = e;code = 101
char = l;code = 108
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = ;code = 32
char = u;code = 117
char = s;code = 115
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = d;code = 100
char = i;code = 105
char = c;code = 99
char = t;code = 116
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = b;code = 98
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = n;code = 110
char = e;code = 101
char = w;code = 119
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = t;code = 116
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = o;code = 111
char = p;code = 112
char = m;code = 109
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = p;code = 112
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = y;code = 121
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = s;code = 115
char = t;code = 116
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = i;code = 105
char = m;code = 109
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = v;code = 118
char = o;code = 111
char = l;code = 108
char = v;code = 118
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = b;code = 98
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = c;code = 99
char = h;code = 104
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = e;code = 101
char = n;code = 110
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = .;code = 46
result sql = AIMS : Mathematical models were created which predict the growth of spoilage bacteria in response to various preservation systems.

METHODS AND RESULTS : A Box-Behnken design included five variables: pH (2.8, 3.3, 3.8), titratable acidity (0.20%, 0.40%, 0.60%), sugar (8.0, 12.0, 16.0 * Brix), sodium benzoate concentration (100, 225, 350 ppm), and potassium sorbate concentration (100, 225, 350 ppm). Duplicate samples were inoculated with a bacterial cocktail (100 microl 50 ml(-1)) consisting of equal proportions of Acinetobacter calcoaceticus and Gluconobacter oxydans (5 x 10(5) cfu ml(-1) each). Bacteria from the inoculated samples were enumerated on malt extract agar at zero, one, two, four, six, and eight weeks.

CONCLUSION : The pH, titratable acidity, sugar content, sodium benzoate, and potassium sorbate levels were all significant factors in predicting the growth of spoilage bacteria.

SIGNIFICANCE AND IMPACT OF THE STUDY : This beverage spoilage model can be used to predict microbial stability in new beverage product development and potentially reduce the cost and time involved in microbial challenge testing.

Publication: J. Appl. Microbiol. 2001 Aug ;91(2)::237-47.
Publication Date: 2001 Aug
Study Author(s): Battey, A S;Schaffner, D W;
Institution: Food Risk Analysis Initiative, Rutgers, The State University of New Jersey, New Brunswick, 08901-8520, USA.
Shortcut link to this study:
AIMS : Mathematical models were created which predict the growth of spoilage bacteria in response to various preservation systems.

METHODS AND RESULTS : A Box-Behnken design included five variables: pH (2.8, 3.3, 3.8), titratable acidity (0.20%, 0.40%, 0.60%), sugar (8.0, 12.0, 16.0 * Brix), sodium benzoate concentration (100, 225, 350 ppm), and potassium sorbate concentration (100, 225, 350 ppm). Duplicate samples were inoculated with a bacterial cocktail (100 microl 50 ml(-1)) consisting of equal proportions of Acinetobacter calcoaceticus and Gluconobacter oxydans (5 x 10(5) cfu ml(-1) each). Bacteria from the inoculated samples were enumerated on malt extract agar at zero, one, two, four, six, and eight weeks.

CONCLUSION : The pH, titratable acidity, sugar content, sodium benzoate, and potassium sorbate levels were all significant factors in predicting the growth of spoilage bacteria.

SIGNIFICANCE AND IMPACT OF THE STUDY : This beverage spoilage model can be used to predict microbial stability in new beverage product development and potentially reduce the cost and time involved in microbial challenge testing.
PMID: 11473588

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