char = R;code = 82 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = c;code = 99 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = P;code = 80 char = s;code = 115 char = e;code = 101 char = u;code = 117 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = s;code = 115 char = .;code = 46 result sql = Role of dose concentration in biocide efficacy against Pseudomonas aeruginosa biofilms.
Role of dose concentration in biocide efficacy against Pseudomonas aeruginosa biofilms.char = P;code = 80 char = s;code = 115 char = e;code = 101 char = u;code = 117 char = d;code = 100 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = e;code = 101 char = r;code = 114 char = u;code = 117 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = o;code = 111 char = s;code = 115 char = a;code = 97 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = p;code = 112 char = p;code = 112 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = a;code = 97 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = k;code = 107 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = l;code = 108 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = h;code = 104 char = y;code = 121 char = d;code = 100 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = 2;code = 50 char = ,;code = 44 char = 2;code = 50 char = -;code = 45 char = d;code = 100 char = i;code = 105 char = b;code = 98 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = o;code = 111 char = -;code = 45 char = 3;code = 51 char = -;code = 45 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = l;code = 108 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = m;code = 109 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = D;code = 68 char = B;code = 66 char = N;code = 78 char = P;code = 80 char = A;code = 65 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = k;code = 107 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = n;code = 110 char = z;code = 122 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = (;code = 40 char = A;code = 65 char = D;code = 68 char = B;code = 66 char = A;code = 65 char = C;code = 67 char = );code = 41 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = g;code = 103 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = q;code = 113 char = u;code = 117 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = k;code = 107 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = n;code = 110 char = k;code = 107 char = t;code = 116 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = a;code = 97 char = m;code = 109 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = R;code = 82 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = 1;code = 49 char = 2;code = 50 char = 0;code = 48 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = l;code = 108 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = 3;code = 51 char = 4;code = 52 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = h;code = 104 char = y;code = 121 char = d;code = 100 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = 2;code = 50 char = 9;code = 57 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = D;code = 68 char = B;code = 66 char = N;code = 78 char = P;code = 80 char = A;code = 65 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = 1;code = 49 char = 9;code = 57 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = A;code = 65 char = D;code = 68 char = B;code = 66 char = A;code = 65 char = C;code = 67 char = .;code = 46 char = ;code = 32 char = I;code = 73 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = k;code = 107 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = n;code = 110 char = k;code = 107 char = t;code = 116 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = m;code = 109 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = k;code = 107 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = t;code = 116 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = l;code = 108 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = f;code = 102 char = f;code = 102 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = l;code = 108 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = h;code = 104 char = d;code = 100 char = y;code = 121 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = D;code = 68 char = B;code = 66 char = N;code = 78 char = P;code = 80 char = A;code = 65 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = s;code = 115 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = -;code = 45 char = k;code = 107 char = i;code = 105 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = n;code = 110 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = a;code = 97 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = h;code = 104 char = a;code = 97 char = p;code = 112 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = r;code = 114 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = r;code = 114 char = d;code = 100 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = b;code = 98 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = l;code = 108 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = g;code = 103 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = l;code = 108 char = d;code = 100 char = e;code = 101 char = h;code = 104 char = y;code = 121 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = b;code = 98 char = p;code = 112 char = o;code = 111 char = p;code = 112 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = D;code = 68 char = B;code = 66 char = N;code = 78 char = P;code = 80 char = A;code = 65 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = A;code = 65 char = D;code = 68 char = B;code = 66 char = A;code = 65 char = C;code = 67 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = f;code = 102 char = i;code = 105 char = l;code = 108 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = o;code = 111 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = o;code = 111 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Pseudomonas aeruginosa entrapped in alginate gel beads to form artificial biofilms resisted killing by chlorine, glutaraldehyde, 2,2-dibromo-3-nitrilopropionamide (DBNPA), and an alkyl dimethyl benzyl ammonium compound (ADBAC). The degree of resistance was quantified by a resistance factor that compared killing times for biofilm and planktonic cells in response to the same concentration of antimicrobial agent. Resistance factors averaged 120 for chlorine, 34 for glutaraldehyde, 29 for DBNPA, and 1900 for ADBAC. In every case, resistance factors decreased with increasing concentration of the antimicrobial agent. An independent analysis of the concentration dependence of the apparent rates of killing of planktonic and biofilm bacteria showed that elevating the treatment concentration increased bacterial killing more in the biofilm than it did in a suspension culture. Calculation of a transport modulus comparing the rates of biocide reaction and diffusion suggested that at least part of the biofilm resistance to chlorine, glutaraldehdye, and DBNPA could be attributed to incomplete or slow penetration of these agents into the biofilm. Time-kill curves were nonlinear for biofilm bacteria in some cases. The shapes of these curves implicated retarded antimicrobial penetration for chlorine and glutaraldehyde and the presence of a tolerant subpopulation for DBNPA and ADBAC. The results indicate that treating biofilms with a concentrated dose of biocide is more effective than using prolonged doses of a lower concentration.
Publication: J. Ind. Microbiol. Biotechnol. 2002 Jul ;29(1)::10-5.
Publication Date: 2002 Jul
Study Author(s): Grobe, K J;Zahller, J;Stewart, P S;
Institution: Center for Biofilm Engineering and Department of Chemical Engineering, Montana State University, Bozeman, MT 59717-3980, USA.
Shortcut link to this study:
Pseudomonas aeruginosa entrapped in alginate gel beads to form artificial biofilms resisted killing by chlorine, glutaraldehyde, 2,2-dibromo-3-nitrilopropionamide (DBNPA), and an alkyl dimethyl benzyl ammonium compound (ADBAC). The degree of resistance was quantified by a resistance factor that compared killing times for biofilm and planktonic cells in response to the same concentration of antimicrobial agent. Resistance factors averaged 120 for chlorine, 34 for glutaraldehyde, 29 for DBNPA, and 1900 for ADBAC. In every case, resistance factors decreased with increasing concentration of the antimicrobial agent. An independent analysis of the concentration dependence of the apparent rates of killing of planktonic and biofilm bacteria showed that elevating the treatment concentration increased bacterial killing more in the biofilm than it did in a suspension culture. Calculation of a transport modulus comparing the rates of biocide reaction and diffusion suggested that at least part of the biofilm resistance to chlorine, glutaraldehdye, and DBNPA could be attributed to incomplete or slow penetration of these agents into the biofilm. Time-kill curves were nonlinear for biofilm bacteria in some cases. The shapes of these curves implicated retarded antimicrobial penetration for chlorine and glutaraldehyde and the presence of a tolerant subpopulation for DBNPA and ADBAC. The results indicate that treating biofilms with a concentrated dose of biocide is more effective than using prolonged doses of a lower concentration.
PMID: 12080421