char = C;code = 67 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = i;code = 105 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = -;code = 45 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = o;code = 111 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = z;code = 122 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = .;code = 46 result sql = Comparative efficiencies of isopropyl and tert-butyl alcohols for extracting zeins from maize endosperm.
Comparative efficiencies of isopropyl and tert-butyl alcohols for extracting zeins from maize endosperm.char = P;code = 80 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = ;code = 32 char = g;code = 103 char = r;code = 114 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = i;code = 105 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = e;code = 101 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = l;code = 108 char = d;code = 100 char = -;code = 45 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = b;code = 98 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = i;code = 105 char = r;code = 114 char = ;code = 32 char = o;code = 111 char = p;code = 112 char = a;code = 97 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = -;code = 45 char = 2;code = 50 char = ;code = 32 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = u;code = 117 char = b;code = 98 char = i;code = 105 char = l;code = 108 char = i;code = 105 char = z;code = 122 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = );code = 41 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = e;code = 101 char = q;code = 113 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 5;code = 53 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = N;code = 78 char = a;code = 97 char = C;code = 67 char = l;code = 108 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = (;code = 40 char = 1;code = 49 char = ,;code = 44 char = 2;code = 50 char = );code = 41 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = o;code = 111 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = (;code = 40 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = );code = 41 char = .;code = 46 char = ;code = 32 char = Z;code = 90 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = o;code = 111 char = b;code = 98 char = t;code = 116 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = 5;code = 53 char = 5;code = 53 char = %;code = 37 char = ;code = 32 char = (;code = 40 char = w;code = 119 char = /;code = 47 char = w;code = 119 char = );code = 41 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = p;code = 112 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = o;code = 111 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = (;code = 40 char = i;code = 105 char = -;code = 45 char = P;code = 80 char = r;code = 114 char = O;code = 79 char = H;code = 72 char = );code = 41 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 2;code = 50 char = %;code = 37 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = r;code = 114 char = e;code = 101 char = i;code = 105 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = (;code = 40 char = D;code = 68 char = T;code = 84 char = T;code = 84 char = );code = 41 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 5;code = 53 char = ;code = 32 char = M;code = 77 char = ;code = 32 char = N;code = 78 char = a;code = 97 char = C;code = 67 char = l;code = 108 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 2;code = 50 char = %;code = 37 char = ;code = 32 char = D;code = 68 char = T;code = 84 char = T;code = 84 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = H;code = 72 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = 6;code = 54 char = 0;code = 48 char = %;code = 37 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = t;code = 116 char = -;code = 45 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = y;code = 121 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = o;code = 111 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = ;code = 32 char = (;code = 40 char = t;code = 116 char = -;code = 45 char = B;code = 66 char = u;code = 117 char = O;code = 79 char = H;code = 72 char = );code = 41 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 2;code = 50 char = %;code = 37 char = ;code = 32 char = D;code = 68 char = T;code = 84 char = T;code = 84 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = l;code = 108 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 5;code = 53 char = %;code = 37 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 2;code = 50 char = %;code = 37 char = ;code = 32 char = D;code = 68 char = T;code = 84 char = T;code = 84 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = 3;code = 51 char = 0;code = 48 char = %;code = 37 char = ;code = 32 char = t;code = 116 char = -;code = 45 char = B;code = 66 char = u;code = 117 char = O;code = 79 char = H;code = 72 char = .;code = 46 char = ;code = 32 char = F;code = 70 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = g;code = 103 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = z;code = 122 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = o;code = 111 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = s;code = 115 char = l;code = 108 char = i;code = 105 char = g;code = 103 char = h;code = 104 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = f;code = 102 char = f;code = 102 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = :;code = 58 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = -;code = 45 char = B;code = 66 char = u;code = 117 char = O;code = 79 char = H;code = 72 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = b;code = 98 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = t;code = 116 char = u;code = 117 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = -;code = 45 char = P;code = 80 char = r;code = 114 char = O;code = 79 char = H;code = 72 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = i;code = 105 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = t;code = 116 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = o;code = 111 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = ;code = 32 char = n;code = 110 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = F;code = 70 char = (;code = 40 char = I;code = 73 char = I;code = 73 char = );code = 41 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = F;code = 70 char = (;code = 40 char = I;code = 73 char = I;code = 73 char = I;code = 73 char = );code = 41 char = ;code = 32 char = +;code = 43 char = ;code = 32 char = F;code = 70 char = (;code = 40 char = I;code = 73 char = V;code = 86 char = );code = 41 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = o;code = 111 char = r;code = 114 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = L;code = 76 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = r;code = 114 char = y;code = 121 char = -;code = 45 char = M;code = 77 char = o;code = 111 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = u;code = 117 char = x;code = 120 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = <;code = 60 char = b;code = 98 char = >;code = 62 char = ;code = 32 char = S;code = 83 char = D;code = 68 char = S;code = 83 char = -;code = 45 char = P;code = 80 char = A;code = 65 char = G;code = 71 char = E;code = 69 char = ;code = 32 char = :;code = 58 char = ;code = 32 char = <;code = 60 char = /;code = 47 char = b;code = 98 char = >;code = 62 char = a;code = 97 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = y;code = 121 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = a;code = 97 char = l;code = 108 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = ;code = 32 char = t;code = 116 char = y;code = 121 char = p;code = 112 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = z;code = 122 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = (;code = 40 char = a;code = 97 char = l;code = 108 char = p;code = 112 char = h;code = 104 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = g;code = 103 char = a;code = 97 char = m;code = 109 char = m;code = 109 char = a;code = 97 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = l;code = 108 char = t;code = 116 char = a;code = 97 char = );code = 41 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 3;code = 51 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = F;code = 70 char = (;code = 40 char = I;code = 73 char = I;code = 73 char = I;code = 73 char = );code = 41 char = ,;code = 44 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = d;code = 100 char = u;code = 117 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = z;code = 122 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = -;code = 45 char = B;code = 66 char = u;code = 117 char = O;code = 79 char = H;code = 72 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = k;code = 107 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = E;code = 69 char = (;code = 40 char = 4;code = 52 char = );code = 41 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = F;code = 70 char = (;code = 40 char = I;code = 73 char = V;code = 86 char = );code = 41 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = N;code = 78 char = a;code = 97 char = C;code = 67 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = H;code = 72 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = i;code = 105 char = s;code = 115 char = c;code = 99 char = u;code = 117 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = g;code = 103 char = a;code = 97 char = r;code = 114 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = f;code = 102 char = l;code = 108 char = u;code = 117 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = y;code = 121 char = i;code = 105 char = e;code = 101 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = z;code = 122 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = c;code = 99 char = o;code = 111 char = h;code = 104 char = o;code = 111 char = l;code = 108 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Protein of endosperm of maize grains originating from three wild-type inbreds and their opaque-2 versions were solubilized in diverse extracts (E) by the sequential use of 0.5 M NaCl, water (E(1,2)), alcohol plus a reducing agent (E(3)), and salt plus a reducing agent (E(4)). Zeins were isolated in extracts E(3) and E(4) obtained by using 55% (w/w) isopropyl alcohol (i-PrOH) + 0.2% dithiothreitol (DTT) followed by 0.5 M NaCl + 0.2% DTT buffered at pH 10 or 60% tert-butyl alcohol (t-BuOH) + 0.2% DTT followed by 0.5% sodium acetate + 0.2% DTT in 30% t-BuOH. For a given genotype the percentage of extracted zeins was independent of the nature of the alcohol. The latter had a slight effect on the respective magnitude of E(3) and E(4): E(3) increased at the expense of E(4) when t-BuOH was substituted to i-PrOH for their isolation. The percentage of the total endosperm nitrogen present in E(3) + E(4) was identical to that of fractions F(II) + F(III) + F(IV) isolated according to the classical Landry-Moureaux extraction procedure SDS-PAGE : analysis revealed the presence of all types of zeins (alpha, beta, gamma, and delta) in E(3) and F(III), residual zeins in E(4) isolated with t-BuOH, and streaking only in E(4) and F(IV) isolated with NaCl at pH 10. The data together with those of the literature were discussed with regard to the influence of procedure on the yield of zeins using alcoholic extraction.
Publication: J. Agric. Food Chem. 2002 Jul 3 ;50(14)::4131-4.
Publication Date: 2002 Jul 3
Study Author(s): Landry, Jacques;Delhaye, Sonia;Damerval, Catherine;
Institution: Laboratoire de Chimie Biologique, CBAI, INRA, 78850 Thiverval Grignon, France.
[email protected]
Shortcut link to this study:
Protein of endosperm of maize grains originating from three wild-type inbreds and their opaque-2 versions were solubilized in diverse extracts (E) by the sequential use of 0.5 M NaCl, water (E(1,2)), alcohol plus a reducing agent (E(3)), and salt plus a reducing agent (E(4)). Zeins were isolated in extracts E(3) and E(4) obtained by using 55% (w/w) isopropyl alcohol (i-PrOH) + 0.2% dithiothreitol (DTT) followed by 0.5 M NaCl + 0.2% DTT buffered at pH 10 or 60% tert-butyl alcohol (t-BuOH) + 0.2% DTT followed by 0.5% sodium acetate + 0.2% DTT in 30% t-BuOH. For a given genotype the percentage of extracted zeins was independent of the nature of the alcohol. The latter had a slight effect on the respective magnitude of E(3) and E(4): E(3) increased at the expense of E(4) when t-BuOH was substituted to i-PrOH for their isolation. The percentage of the total endosperm nitrogen present in E(3) + E(4) was identical to that of fractions F(II) + F(III) + F(IV) isolated according to the classical Landry-Moureaux extraction procedure SDS-PAGE : analysis revealed the presence of all types of zeins (alpha, beta, gamma, and delta) in E(3) and F(III), residual zeins in E(4) isolated with t-BuOH, and streaking only in E(4) and F(IV) isolated with NaCl at pH 10. The data together with those of the literature were discussed with regard to the influence of procedure on the yield of zeins using alcoholic extraction.
PMID: 12083896