|
|
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result sql = Preservation of cartilage matrix proteoglycans using cationic dyes chemically related to ruthenium hexaammine trichloride.
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result sql = We tested various cationic dyes chemically related to ruthenium hexaammine trichloride (RHT) [i.e., the RHT-cyclohexanedione complex (RHT-CC), pentaamine ruthenium N-dimethylphenylenediimine trichloride (PRT), tris-(bipyridyl)ruthenium (II) chloride (TRC), tris (bipyridyl) iron (II) chloride (TIC), and cobalt hexaammine trichloride (CHT)] for their effectiveness in precipitating cartilage matrix proteoglycans in situ. Dyes were introduced into media at the onset of processing and were present throughout both aldehyde fixation and osmium tetroxide post-fixation. Contrary to expectation, most of the dye-proteoglycan complexes generated and stable under aldehyde fixation conditions were found to be unstable during post-fixation despite the continuing presence of the dye. A similar phenomenon was also found for the cationic dyes commonly used for precipitation of proteoglycans in cartilage tissue sections (such as Acridine Orange, Alcian Blue, Azure A, Methylene Blue, and Ruthenium Red). Only two dyes, i.e., RHT and the newly tested RHT-CC, formed proteoglycan precipitates sufficiently stable to resist disruption and extraction during osmium tetroxide post-fixation. The latter may be particularly useful in semiquantitative analyses of proteoglycan content in unstained tissue sections owing to its intense brown-black color. For applications in which the osmium tetroxide post-fixation step may be omitted, TRC and PRT may also be valuable for semiquantitative histochemistry by virtue of their stable fluorescence and intense violet color signals, respectively.
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char = n;code = 110
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char = u;code = 117
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char = a;code = 97
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char = m;code = 109
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char = c;code = 99
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char = l;code = 108
char = o;code = 111
char = r;code = 114
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = .;code = 46
result sql = Preservation of cartilage matrix proteoglycans using cationic dyes chemically related to ruthenium hexaammine trichloride.
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = e;code = 101
char = s;code = 115
char = t;code = 116
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char = i;code = 105
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char = i;code = 105
char = c;code = 99
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char = d;code = 100
char = y;code = 121
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char = s;code = 115
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char = h;code = 104
char = e;code = 101
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = l;code = 108
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char = l;code = 108
char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = t;code = 116
char = o;code = 111
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char = h;code = 104
char = e;code = 101
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char = u;code = 117
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char = h;code = 104
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char = a;code = 97
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char = m;code = 109
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char = e;code = 101
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char = c;code = 99
char = h;code = 104
char = l;code = 108
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char = r;code = 114
char = i;code = 105
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char = (;code = 40
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char = H;code = 72
char = T;code = 84
char = );code = 41
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char = [;code = 91
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char = .;code = 46
char = e;code = 101
char = .;code = 46
char = ,;code = 44
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char = e;code = 101
char = ;code = 32
char = R;code = 82
char = H;code = 72
char = T;code = 84
char = -;code = 45
char = c;code = 99
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char = c;code = 99
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char = o;code = 111
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char = e;code = 101
char = x;code = 120
char = a;code = 97
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = e;code = 101
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char = c;code = 99
char = o;code = 111
char = m;code = 109
char = p;code = 112
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char = e;code = 101
char = x;code = 120
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char = (;code = 40
char = R;code = 82
char = H;code = 72
char = T;code = 84
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char = C;code = 67
char = C;code = 67
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = p;code = 112
char = e;code = 101
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char = t;code = 116
char = a;code = 97
char = a;code = 97
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = r;code = 114
char = u;code = 117
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = n;code = 110
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = N;code = 78
char = -;code = 45
char = d;code = 100
char = i;code = 105
char = m;code = 109
char = e;code = 101
char = t;code = 116
char = h;code = 104
char = y;code = 121
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char = p;code = 112
char = h;code = 104
char = e;code = 101
char = n;code = 110
char = y;code = 121
char = l;code = 108
char = e;code = 101
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char = e;code = 101
char = d;code = 100
char = i;code = 105
char = i;code = 105
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = c;code = 99
char = h;code = 104
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = R;code = 82
char = T;code = 84
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = s;code = 115
char = -;code = 45
char = (;code = 40
char = b;code = 98
char = i;code = 105
char = p;code = 112
char = y;code = 121
char = r;code = 114
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char = d;code = 100
char = y;code = 121
char = l;code = 108
char = );code = 41
char = r;code = 114
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char = t;code = 116
char = h;code = 104
char = e;code = 101
char = n;code = 110
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = (;code = 40
char = I;code = 73
char = I;code = 73
char = );code = 41
char = ;code = 32
char = c;code = 99
char = h;code = 104
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = i;code = 105
char = d;code = 100
char = e;code = 101
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char = (;code = 40
char = T;code = 84
char = R;code = 82
char = C;code = 67
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = t;code = 116
char = r;code = 114
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char = s;code = 115
char = ;code = 32
char = (;code = 40
char = b;code = 98
char = i;code = 105
char = p;code = 112
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char = i;code = 105
char = d;code = 100
char = y;code = 121
char = l;code = 108
char = );code = 41
char = ;code = 32
char = i;code = 105
char = r;code = 114
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = (;code = 40
char = I;code = 73
char = I;code = 73
char = );code = 41
char = ;code = 32
char = c;code = 99
char = h;code = 104
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = T;code = 84
char = I;code = 73
char = C;code = 67
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = b;code = 98
char = a;code = 97
char = l;code = 108
char = t;code = 116
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char = h;code = 104
char = e;code = 101
char = x;code = 120
char = a;code = 97
char = a;code = 97
char = m;code = 109
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = c;code = 99
char = h;code = 104
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = C;code = 67
char = H;code = 72
char = T;code = 84
char = );code = 41
char = ];code = 93
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = i;code = 105
char = r;code = 114
char = ;code = 32
char = e;code = 101
char = f;code = 102
char = f;code = 102
char = e;code = 101
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char = t;code = 116
char = i;code = 105
char = v;code = 118
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char = n;code = 110
char = e;code = 101
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char = s;code = 115
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = c;code = 99
char = i;code = 105
char = p;code = 112
char = i;code = 105
char = t;code = 116
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = c;code = 99
char = a;code = 97
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char = t;code = 116
char = i;code = 105
char = l;code = 108
char = a;code = 97
char = g;code = 103
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = x;code = 120
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = o;code = 111
char = g;code = 103
char = l;code = 108
char = y;code = 121
char = c;code = 99
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = s;code = 115
char = i;code = 105
char = t;code = 116
char = u;code = 117
char = .;code = 46
char = ;code = 32
char = D;code = 68
char = y;code = 121
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = o;code = 111
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char = m;code = 109
char = e;code = 101
char = d;code = 100
char = i;code = 105
char = a;code = 97
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char = a;code = 97
char = t;code = 116
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = e;code = 101
char = t;code = 116
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = c;code = 99
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = n;code = 110
char = g;code = 103
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
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char = r;code = 114
char = e;code = 101
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char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
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char = t;code = 116
char = ;code = 32
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char = h;code = 104
char = r;code = 114
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char = g;code = 103
char = h;code = 104
char = o;code = 111
char = u;code = 117
char = t;code = 116
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char = b;code = 98
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char = a;code = 97
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char = d;code = 100
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char = i;code = 105
char = o;code = 111
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char = .;code = 46
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char = C;code = 67
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char = r;code = 114
char = a;code = 97
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char = a;code = 97
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char = i;code = 105
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char = t;code = 116
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char = a;code = 97
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char = i;code = 105
char = o;code = 111
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char = c;code = 99
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char = d;code = 100
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char = e;code = 101
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char = t;code = 116
char = o;code = 111
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char = b;code = 98
char = e;code = 101
char = ;code = 32
char = u;code = 117
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char = s;code = 115
char = t;code = 116
char = a;code = 97
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char = l;code = 108
char = e;code = 101
char = ;code = 32
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char = u;code = 117
char = r;code = 114
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char = n;code = 110
char = g;code = 103
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char = p;code = 112
char = o;code = 111
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char = i;code = 105
char = o;code = 111
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char = h;code = 104
char = e;code = 101
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char = e;code = 101
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char = i;code = 105
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char = m;code = 109
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char = p;code = 112
char = r;code = 114
char = e;code = 101
char = c;code = 99
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char = i;code = 105
char = n;code = 110
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char = t;code = 116
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char = i;code = 105
char = n;code = 110
char = ;code = 32
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char = n;code = 110
char = s;code = 115
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char = c;code = 99
char = k;code = 107
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = l;code = 108
char = o;code = 111
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char = .;code = 46
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char = o;code = 111
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char = ;code = 32
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char = p;code = 112
char = p;code = 112
char = l;code = 108
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = s;code = 115
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = ;code = 32
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char = i;code = 105
char = c;code = 99
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char = ;code = 32
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char = i;code = 105
char = o;code = 111
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char = ;code = 32
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char = e;code = 101
char = p;code = 112
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = y;code = 121
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = ;code = 32
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char = m;code = 109
char = i;code = 105
char = t;code = 116
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char = e;code = 101
char = d;code = 100
char = ,;code = 44
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char = R;code = 82
char = C;code = 67
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = P;code = 80
char = R;code = 82
char = T;code = 84
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = y;code = 121
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char = l;code = 108
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char = o;code = 111
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char = e;code = 101
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char = a;code = 97
char = l;code = 108
char = u;code = 117
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = m;code = 109
char = i;code = 105
char = q;code = 113
char = u;code = 117
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char = t;code = 116
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char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
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char = i;code = 105
char = s;code = 115
char = t;code = 116
char = o;code = 111
char = c;code = 99
char = h;code = 104
char = e;code = 101
char = m;code = 109
char = i;code = 105
char = s;code = 115
char = t;code = 116
char = r;code = 114
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char = y;code = 121
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char = u;code = 117
char = e;code = 101
char = ;code = 32
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char = f;code = 102
char = ;code = 32
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char = h;code = 104
char = e;code = 101
char = i;code = 105
char = r;code = 114
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = e;code = 101
char = ;code = 32
char = f;code = 102
char = l;code = 108
char = u;code = 117
char = o;code = 111
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = c;code = 99
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = n;code = 110
char = s;code = 115
char = e;code = 101
char = ;code = 32
char = v;code = 118
char = i;code = 105
char = o;code = 111
char = l;code = 108
char = e;code = 101
char = t;code = 116
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = l;code = 108
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = s;code = 115
char = i;code = 105
char = g;code = 103
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = s;code = 115
char = ,;code = 44
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = p;code = 112
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = l;code = 108
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result sql = We tested various cationic dyes chemically related to ruthenium hexaammine trichloride (RHT) [i.e., the RHT-cyclohexanedione complex (RHT-CC), pentaamine ruthenium N-dimethylphenylenediimine trichloride (PRT), tris-(bipyridyl)ruthenium (II) chloride (TRC), tris (bipyridyl) iron (II) chloride (TIC), and cobalt hexaammine trichloride (CHT)] for their effectiveness in precipitating cartilage matrix proteoglycans in situ. Dyes were introduced into media at the onset of processing and were present throughout both aldehyde fixation and osmium tetroxide post-fixation. Contrary to expectation, most of the dye-proteoglycan complexes generated and stable under aldehyde fixation conditions were found to be unstable during post-fixation despite the continuing presence of the dye. A similar phenomenon was also found for the cationic dyes commonly used for precipitation of proteoglycans in cartilage tissue sections (such as Acridine Orange, Alcian Blue, Azure A, Methylene Blue, and Ruthenium Red). Only two dyes, i.e., RHT and the newly tested RHT-CC, formed proteoglycan precipitates sufficiently stable to resist disruption and extraction during osmium tetroxide post-fixation. The latter may be particularly useful in semiquantitative analyses of proteoglycan content in unstained tissue sections owing to its intense brown-black color. For applications in which the osmium tetroxide post-fixation step may be omitted, TRC and PRT may also be valuable for semiquantitative histochemistry by virtue of their stable fluorescence and intense violet color signals, respectively.
Publication: J. Histochem. Cytochem. 1992 Jul ;40(7)::909-17.
Publication Date: 1992 Jul
Study Author(s): Hunziker, E B;Ludi, A;Herrmann, W;
Institution: M. E. Müller Institute for Biomechanics, University of Bern, Switzerland.
Shortcut link to this study:
Publication Date: 1992 Jul
Study Author(s): Hunziker, E B;Ludi, A;Herrmann, W;
Institution: M. E. Müller Institute for Biomechanics, University of Bern, Switzerland.
Shortcut link to this study:
We tested various cationic dyes chemically related to ruthenium hexaammine trichloride (RHT) [i.e., the RHT-cyclohexanedione complex (RHT-CC), pentaamine ruthenium N-dimethylphenylenediimine trichloride (PRT), tris-(bipyridyl)ruthenium (II) chloride (TRC), tris (bipyridyl) iron (II) chloride (TIC), and cobalt hexaammine trichloride (CHT)] for their effectiveness in precipitating cartilage matrix proteoglycans in situ. Dyes were introduced into media at the onset of processing and were present throughout both aldehyde fixation and osmium tetroxide post-fixation. Contrary to expectation, most of the dye-proteoglycan complexes generated and stable under aldehyde fixation conditions were found to be unstable during post-fixation despite the continuing presence of the dye. A similar phenomenon was also found for the cationic dyes commonly used for precipitation of proteoglycans in cartilage tissue sections (such as Acridine Orange, Alcian Blue, Azure A, Methylene Blue, and Ruthenium Red). Only two dyes, i.e., RHT and the newly tested RHT-CC, formed proteoglycan precipitates sufficiently stable to resist disruption and extraction during osmium tetroxide post-fixation. The latter may be particularly useful in semiquantitative analyses of proteoglycan content in unstained tissue sections owing to its intense brown-black color. For applications in which the osmium tetroxide post-fixation step may be omitted, TRC and PRT may also be valuable for semiquantitative histochemistry by virtue of their stable fluorescence and intense violet color signals, respectively.
PMID: 1376743
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