char = E;code = 69 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = s;code = 115 char = y;code = 121 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = .;code = 46 result sql = Engineering ketocarotenoid biosynthesis in potato tubers.
Engineering ketocarotenoid biosynthesis in potato tubers.char = C;code = 67 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = u;code = 117 char = m;code = 109 char = p;code = 112 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = x;code = 120 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = s;code = 115 char = o;code = 111 char = c;code = 99 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = h;code = 104 char = e;code = 101 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = f;code = 102 char = i;code = 105 char = t;code = 116 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = A;code = 65 char = t;code = 116 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = p;code = 112 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = s;code = 115 char = y;code = 121 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = c;code = 99 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = f;code = 102 char = u;code = 117 char = l;code = 108 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = t;code = 116 char = h;code = 104 char = o;code = 111 char = u;code = 117 char = g;code = 103 char = h;code = 104 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = n;code = 110 char = o;code = 111 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = n;code = 110 char = u;code = 117 char = t;code = 116 char = r;code = 114 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = s;code = 115 char = i;code = 105 char = g;code = 103 char = n;code = 110 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = x;code = 120 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = a;code = 97 char = g;code = 103 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = g;code = 103 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = u;code = 117 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = t;code = 116 char = u;code = 117 char = d;code = 100 char = y;code = 121 char = ,;code = 44 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = i;code = 105 char = o;code = 111 char = s;code = 115 char = y;code = 121 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = g;code = 103 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = p;code = 112 char = o;code = 111 char = t;code = 116 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = B;code = 66 char = o;code = 111 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = S;code = 83 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = n;code = 110 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = S;code = 83 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = n;code = 110 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = j;code = 106 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = o;code = 111 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = g;code = 103 char = a;code = 97 char = l;code = 108 char = ;code = 32 char = b;code = 98 char = k;code = 107 char = t;code = 116 char = 1;code = 49 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = t;code = 116 char = a;code = 97 char = -;code = 45 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = w;code = 119 char = o;code = 111 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = j;code = 106 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = c;code = 99 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ,;code = 44 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = l;code = 108 char = u;code = 117 char = t;code = 116 char = e;code = 101 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = x;code = 120 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = u;code = 117 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = x;code = 120 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = .;code = 46 char = ;code = 32 char = 1;code = 49 char = 4;code = 52 char = ;code = 32 char = m;code = 109 char = i;code = 105 char = c;code = 99 char = r;code = 114 char = o;code = 111 char = g;code = 103 char = ;code = 32 char = g;code = 103 char = (;code = 40 char = -;code = 45 char = 1;code = 49 char = );code = 41 char = ;code = 32 char = D;code = 68 char = W;code = 87 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = s;code = 115 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = k;code = 107 char = t;code = 116 char = 1;code = 49 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = .;code = 46 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = j;code = 106 char = a;code = 97 char = ;code = 32 char = b;code = 98 char = u;code = 117 char = t;code = 116 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = S;code = 83 char = .;code = 46 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = b;code = 98 char = a;code = 97 char = c;code = 99 char = k;code = 107 char = g;code = 103 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = .;code = 46 char = ;code = 32 char = C;code = 67 char = o;code = 111 char = -;code = 45 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = m;code = 109 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = .;code = 46 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = c;code = 99 char = r;code = 114 char = t;code = 116 char = B;code = 66 char = ,;code = 44 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = o;code = 111 char = d;code = 100 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = p;code = 112 char = h;code = 104 char = y;code = 121 char = t;code = 116 char = o;code = 111 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = s;code = 115 char = y;code = 121 char = n;code = 110 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = k;code = 107 char = t;code = 116 char = 1;code = 49 char = ;code = 32 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = h;code = 104 char = i;code = 105 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = r;code = 114 char = m;code = 109 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = w;code = 119 char = o;code = 111 char = u;code = 117 char = l;code = 108 char = d;code = 100 char = ;code = 32 char = e;code = 101 char = n;code = 110 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = l;code = 108 char = e;code = 101 char = v;code = 118 char = e;code = 101 char = l;code = 108 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = S;code = 83 char = .;code = 46 char = ;code = 32 char = t;code = 116 char = u;code = 117 char = b;code = 98 char = e;code = 101 char = r;code = 114 char = o;code = 111 char = s;code = 115 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = k;code = 107 char = e;code = 101 char = t;code = 116 char = o;code = 111 char = c;code = 99 char = a;code = 97 char = r;code = 114 char = o;code = 111 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = i;code = 105 char = d;code = 100 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Consumption of astaxanthin is increasingly associated with a range of health benefits. Attempts to engineer ketocarotenoid biosynthesis in plants have been successful although there are no reports of nutritionally significant levels of astaxanthin in plant storage organs. Thus, in this study, ketocarotenoid biosynthesis was engineered in potato tubers. Both Solanum tuberosum and Solanum phureja transgenic lines were produced that expressed an algal bkt1 gene, encoding a beta-ketolase, and accumulated ketocarotenoids. Two major ketocarotenoids were detected, ketolutein and astaxanthin. The level of unesterified astaxanthin reached ca. 14 microg g(-1) DW in some bkt1 expressing lines of S. phureja but was much lower in the S. tuberosum background. Co-transformation of S. tuberosum with crtB, encoding phytoene synthase, and the bkt1 gene was achieved in order to determine whether this would enhance the levels of S. tuberosum ketocarotenoid.
Publication: Metab. Eng. 2006 May ;8(3)::253-63.
Publication Date: 2006 May
Study Author(s): Morris, Wayne L;Ducreux, Laurence J M;Fraser, Paul D;Millam, Steve;Taylor, Mark A;
Institution: Quality, Health and Nutrition, Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK.
Shortcut link to this study:
Consumption of astaxanthin is increasingly associated with a range of health benefits. Attempts to engineer ketocarotenoid biosynthesis in plants have been successful although there are no reports of nutritionally significant levels of astaxanthin in plant storage organs. Thus, in this study, ketocarotenoid biosynthesis was engineered in potato tubers. Both Solanum tuberosum and Solanum phureja transgenic lines were produced that expressed an algal bkt1 gene, encoding a beta-ketolase, and accumulated ketocarotenoids. Two major ketocarotenoids were detected, ketolutein and astaxanthin. The level of unesterified astaxanthin reached ca. 14 microg g(-1) DW in some bkt1 expressing lines of S. phureja but was much lower in the S. tuberosum background. Co-transformation of S. tuberosum with crtB, encoding phytoene synthase, and the bkt1 gene was achieved in order to determine whether this would enhance the levels of S. tuberosum ketocarotenoid.
PMID: 16542864