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char = ;code = 32
char = A;code = 65
char = d;code = 100
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char = y;code = 121
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char = a;code = 97
char = c;code = 99
char = i;code = 105
char = d;code = 100
char = s;code = 115
char = .;code = 46
result sql = Molecular cloning and tissue expression of chicken AdipoR1 and AdipoR2 complementary deoxyribonucleic acids.
Molecular cloning and tissue expression of chicken AdipoR1 and AdipoR2 complementary deoxyribonucleic acids.
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char = ;code = 32
char = A;code = 65
char = d;code = 100
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char = A;code = 65
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char = a;code = 97
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char = e;code = 101
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char = s;code = 115
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char = ;code = 32
char = A;code = 65
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char = ,;code = 44
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char = e;code = 101
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char = ;code = 32
char = b;code = 98
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char = A;code = 65
char = d;code = 100
char = i;code = 105
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char = 1;code = 49
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char = o;code = 111
char = r;code = 114
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = i;code = 105
char = p;code = 112
char = o;code = 111
char = R;code = 82
char = 2;code = 50
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char = m;code = 109
char = R;code = 82
char = N;code = 78
char = A;code = 65
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char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
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char = s;code = 115
char = s;code = 115
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char = o;code = 111
char = n;code = 110
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = v;code = 118
char = a;code = 97
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char = o;code = 111
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char = s;code = 115
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char = t;code = 116
char = i;code = 105
char = s;code = 115
char = s;code = 115
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char = e;code = 101
char = s;code = 115
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char = w;code = 119
char = a;code = 97
char = s;code = 115
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char = q;code = 113
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char = f;code = 102
char = i;code = 105
char = e;code = 101
char = d;code = 100
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char = b;code = 98
char = y;code = 121
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char = r;code = 114
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char = a;code = 97
char = l;code = 108
char = -;code = 45
char = t;code = 116
char = i;code = 105
char = m;code = 109
char = e;code = 101
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char = q;code = 113
char = u;code = 117
char = a;code = 97
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = t;code = 116
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
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char = P;code = 80
char = C;code = 67
char = R;code = 82
char = ,;code = 44
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = A;code = 65
char = d;code = 100
char = i;code = 105
char = p;code = 112
char = o;code = 111
char = R;code = 82
char = 1;code = 49
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char = m;code = 109
char = R;code = 82
char = N;code = 78
char = A;code = 65
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
char = e;code = 101
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = h;code = 104
char = i;code = 105
char = g;code = 103
char = h;code = 104
char = e;code = 101
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char = t;code = 116
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char = s;code = 115
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char = e;code = 101
char = l;code = 108
char = e;code = 101
char = t;code = 116
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = m;code = 109
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char = s;code = 115
char = c;code = 99
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char = e;code = 101
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char = ;code = 32
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = p;code = 112
char = o;code = 111
char = s;code = 115
char = e;code = 101
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char = t;code = 116
char = i;code = 105
char = s;code = 115
char = s;code = 115
char = u;code = 117
char = e;code = 101
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = d;code = 100
char = i;code = 105
char = e;code = 101
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char = c;code = 99
char = e;code = 101
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char = a;code = 97
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char = o;code = 111
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = l;code = 108
char = l;code = 108
char = o;code = 111
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
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char = k;code = 107
char = i;code = 105
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char = a;code = 97
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char = ,;code = 44
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char = v;code = 118
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char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = p;code = 112
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char = t;code = 116
char = u;code = 117
char = i;code = 105
char = t;code = 116
char = a;code = 97
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char = y;code = 121
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char = l;code = 108
char = a;code = 97
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char = ,;code = 44
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char = s;code = 115
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char = l;code = 108
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char = o;code = 111
char = R;code = 82
char = 2;code = 50
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char = m;code = 109
char = R;code = 82
char = N;code = 78
char = A;code = 65
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char = e;code = 101
char = x;code = 120
char = p;code = 112
char = r;code = 114
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char = s;code = 115
char = s;code = 115
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char = o;code = 111
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char = a;code = 97
char = s;code = 115
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char = s;code = 115
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char = W;code = 87
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char = a;code = 97
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char = s;code = 115
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char = i;code = 105
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char = a;code = 97
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char = d;code = 100
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char = A;code = 65
char = d;code = 100
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char = p;code = 112
char = o;code = 111
char = R;code = 82
char = 1;code = 49
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char = m;code = 109
char = R;code = 82
char = N;code = 78
char = A;code = 65
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char = q;code = 113
char = u;code = 117
char = a;code = 97
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char = t;code = 116
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char = l;code = 108
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char = o;code = 111
char = R;code = 82
char = 2;code = 50
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char = m;code = 109
char = R;code = 82
char = N;code = 78
char = A;code = 65
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char = q;code = 113
char = u;code = 117
char = a;code = 97
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char = t;code = 116
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char = P;code = 80
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char = W;code = 87
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char = c;code = 99
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char = A;code = 65
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char = R;code = 82
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char = g;code = 103
char = e;code = 101
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char = e;code = 101
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char = u;code = 117
char = b;code = 98
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char = e;code = 101
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char = o;code = 111
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char = u;code = 117
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char = a;code = 97
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char = p;code = 112
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char = s;code = 115
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char = t;code = 116
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char = s;code = 115
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char = e;code = 101
char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
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result sql = AdipoR1 and AdipoR2 belong to a novel class of transmembrane receptors that mediate the effects of adiponectin. We have cloned the chicken AdipoR1 and AdipoR2 complementary deoxyribonucleic acids (cDNA) and determined their expression in various tissues. We also investigated the effect of feed deprivation on the expression of AdipoR1 or AdipoR2 mRNA in the chicken diencephalon, liver, anterior pituitary gland, and adipose tissue. The chicken AdipoR1 and AdipoR2 cDNA sequences were 76-83% identical to the respective mammalian sequences. A hydrophobicity analysis of the deduced amino acid sequences of chicken AdipoR1/AdipoR2 revealed seven distinct hydrophobic regions representing seven transmembrane domains. By RT-PCR, we detected AdipoR1 and AdipoR2 mRNA in adipose tissue, liver, anterior pituitary gland, diencephalon, skeletal muscle, kidney, spleen, ovary, and blood. AdipoR1 or AdipoR2 mRNA expression in various tissues was quantified by real-time quantitative PCR, and AdipoR1 mRNA expression was the highest in skeletal muscle, adipose tissue and diencephalon, followed by kidney, ovary, liver, anterior pituitary gland, and spleen. AdipoR2 mRNA expression was the highest in adipose tissue followed by skeletal muscle, liver, ovary, diencephalon, anterior pituitary gland, kidney, and spleen. We also found that a 48 h feed deprivation significantly decreased AdipoR1 mRNA quantity in the chicken pituitary gland, while AdipoR2 mRNA quantity was significantly increased in adipose tissue (P<0.05). We conclude that the AdipoR1 and AdipoR2 genes are ubiquitously expressed in chicken tissues and that their expression is altered by feed deprivation in the anterior pituitary gland and adipose tissue.

Publication: Domest. Anim. Endocrinol. 2007 Jul ;33(1)::19-31.
Publication Date: 2007 Jul
Study Author(s): Ramachandran, Ramesh;Ocón-Grove, Olga M;Metzger, Shana L;
Institution: Department of Poultry Science, The Pennsylvania State University, University Park, PA 16802, USA. [email protected]
Shortcut link to this study:
AdipoR1 and AdipoR2 belong to a novel class of transmembrane receptors that mediate the effects of adiponectin. We have cloned the chicken AdipoR1 and AdipoR2 complementary deoxyribonucleic acids (cDNA) and determined their expression in various tissues. We also investigated the effect of feed deprivation on the expression of AdipoR1 or AdipoR2 mRNA in the chicken diencephalon, liver, anterior pituitary gland, and adipose tissue. The chicken AdipoR1 and AdipoR2 cDNA sequences were 76-83% identical to the respective mammalian sequences. A hydrophobicity analysis of the deduced amino acid sequences of chicken AdipoR1/AdipoR2 revealed seven distinct hydrophobic regions representing seven transmembrane domains. By RT-PCR, we detected AdipoR1 and AdipoR2 mRNA in adipose tissue, liver, anterior pituitary gland, diencephalon, skeletal muscle, kidney, spleen, ovary, and blood. AdipoR1 or AdipoR2 mRNA expression in various tissues was quantified by real-time quantitative PCR, and AdipoR1 mRNA expression was the highest in skeletal muscle, adipose tissue and diencephalon, followed by kidney, ovary, liver, anterior pituitary gland, and spleen. AdipoR2 mRNA expression was the highest in adipose tissue followed by skeletal muscle, liver, ovary, diencephalon, anterior pituitary gland, kidney, and spleen. We also found that a 48 h feed deprivation significantly decreased AdipoR1 mRNA quantity in the chicken pituitary gland, while AdipoR2 mRNA quantity was significantly increased in adipose tissue (P<0.05). We conclude that the AdipoR1 and AdipoR2 genes are ubiquitously expressed in chicken tissues and that their expression is altered by feed deprivation in the anterior pituitary gland and adipose tissue.
PMID: 16697136

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