|
|
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result sql = Effect of homobifunctional crosslinkers on nucleic acids delivery ability of PEI nanoparticles.
Effect of homobifunctional crosslinkers on nucleic acids delivery ability of PEI nanoparticles.char = P;code = 80
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char = e;code = 101
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char = r;code = 114
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char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
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char = 9;code = 57
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char = c;code = 99
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char = l;code = 108
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result sql = Polyethylenimine (PEI), a widely used cationic polymeric vector with high transfection efficiency, was converted into nanoparticles by introducing ionic and covalent crosslinkers with varying proportion of 1,6-hexanebisphosphate (HP), adipic acid (AA) and 1,4-butane dialdehyde (BA) to obtain a small library of HP-PEI (HPP), AA-PEI (AAP) and BA-PEI (BAP) nanoparticles, respectively. Particles were characterized by spectroscopic technique as well as physicochemical parameters such as size, morphology, surface charge, effect of crosslinking on buffering capacity and DNA binding ability. The entire series of nanoparticles were compared for their cytoxicity and ability to deliver genes in various cell lines. Among various nanoparticles, AAP-3 nanoparticle/DNA complex exhibited higher transfection efficiency (1.5-7.8 folds) than the native PEI (25kDa) and commercially available transfection reagents, such as GenePorter, GenePorter2, Fugene and Superfect, with cell viability >85%. The highest cell viability was observed with BAP nanoparticles (>95%). Importantly, the transfection activity of nanoparticle/DNA complexes was preserved in the presence of serum. Transfection with GFP-siRNA inhibited expression of transfected GFP gene by approximately 81-92%. All nanoparticle types (HPP, AAP and BAP) required a comparable time for entry into cells and subsequent intracellular passage from the cytoplasm to the nucleus. Intravenous delivery of (99)Tc labeled BAP-2/DNA complex to female Balb/c mice revealed the presence of the complex in most of the organs with the highest retention in liver. In conclusion, HPP, AAP and BAP nanoparticles are safe for efficient gene delivery.
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char = c;code = 99
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char = o;code = 111
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char = a;code = 97
char = l;code = 108
char = ;code = 32
char = c;code = 99
char = r;code = 114
char = o;code = 111
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char = s;code = 115
char = l;code = 108
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char = k;code = 107
char = e;code = 101
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char = ;code = 32
char = n;code = 110
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char = l;code = 108
char = e;code = 101
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = a;code = 97
char = c;code = 99
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char = d;code = 100
char = s;code = 115
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = l;code = 108
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = r;code = 114
char = y;code = 121
char = ;code = 32
char = a;code = 97
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = P;code = 80
char = E;code = 69
char = I;code = 73
char = ;code = 32
char = n;code = 110
char = a;code = 97
char = n;code = 110
char = o;code = 111
char = p;code = 112
char = a;code = 97
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = .;code = 46
result sql = Effect of homobifunctional crosslinkers on nucleic acids delivery ability of PEI nanoparticles.
char = o;code = 111
char = l;code = 108
char = y;code = 121
char = e;code = 101
char = t;code = 116
char = h;code = 104
char = y;code = 121
char = l;code = 108
char = e;code = 101
char = n;code = 110
char = i;code = 105
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = P;code = 80
char = E;code = 69
char = I;code = 73
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = d;code = 100
char = e;code = 101
char = l;code = 108
char = y;code = 121
char = ;code = 32
char = u;code = 117
char = s;code = 115
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = p;code = 112
char = o;code = 111
char = l;code = 108
char = y;code = 121
char = m;code = 109
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = v;code = 118
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = o;code = 111
char = r;code = 114
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char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = h;code = 104
char = i;code = 105
char = g;code = 103
char = h;code = 104
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = n;code = 110
char = s;code = 115
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
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char = o;code = 111
char = n;code = 110
char = ;code = 32
char = e;code = 101
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char = f;code = 102
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char = c;code = 99
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char = e;code = 101
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char = ;code = 32
char = w;code = 119
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char = c;code = 99
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char = v;code = 118
char = e;code = 101
char = r;code = 114
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char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
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char = t;code = 116
char = o;code = 111
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char = n;code = 110
char = a;code = 97
char = n;code = 110
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char = p;code = 112
char = a;code = 97
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char = i;code = 105
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char = l;code = 108
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char = b;code = 98
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char = ;code = 32
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = v;code = 118
char = a;code = 97
char = l;code = 108
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = c;code = 99
char = r;code = 114
char = o;code = 111
char = s;code = 115
char = s;code = 115
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = k;code = 107
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
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char = a;code = 97
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char = y;code = 121
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char = n;code = 110
char = g;code = 103
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = p;code = 112
char = o;code = 111
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = 1;code = 49
char = ,;code = 44
char = 6;code = 54
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char = h;code = 104
char = e;code = 101
char = x;code = 120
char = a;code = 97
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char = e;code = 101
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char = i;code = 105
char = s;code = 115
char = p;code = 112
char = h;code = 104
char = o;code = 111
char = s;code = 115
char = p;code = 112
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = H;code = 72
char = P;code = 80
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = d;code = 100
char = i;code = 105
char = p;code = 112
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = a;code = 97
char = c;code = 99
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char = d;code = 100
char = ;code = 32
char = (;code = 40
char = A;code = 65
char = A;code = 65
char = );code = 41
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = 1;code = 49
char = ,;code = 44
char = 4;code = 52
char = -;code = 45
char = b;code = 98
char = u;code = 117
char = t;code = 116
char = a;code = 97
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = d;code = 100
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = d;code = 100
char = e;code = 101
char = h;code = 104
char = y;code = 121
char = d;code = 100
char = e;code = 101
char = ;code = 32
char = (;code = 40
char = B;code = 66
char = A;code = 65
char = );code = 41
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = o;code = 111
char = b;code = 98
char = t;code = 116
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = s;code = 115
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = b;code = 98
char = r;code = 114
char = a;code = 97
char = r;code = 114
char = y;code = 121
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = H;code = 72
char = P;code = 80
char = -;code = 45
char = P;code = 80
char = E;code = 69
char = I;code = 73
char = ;code = 32
char = (;code = 40
char = H;code = 72
char = P;code = 80
char = P;code = 80
char = );code = 41
char = ,;code = 44
char = ;code = 32
char = A;code = 65
char = A;code = 65
char = -;code = 45
char = P;code = 80
char = E;code = 69
char = I;code = 73
char = ;code = 32
char = (;code = 40
char = A;code = 65
char = A;code = 65
char = P;code = 80
char = );code = 41
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = B;code = 66
char = A;code = 65
char = -;code = 45
char = P;code = 80
char = E;code = 69
char = I;code = 73
char = ;code = 32
char = (;code = 40
char = B;code = 66
char = A;code = 65
char = P;code = 80
char = );code = 41
char = ;code = 32
char = n;code = 110
char = a;code = 97
char = n;code = 110
char = o;code = 111
char = p;code = 112
char = a;code = 97
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = ,;code = 44
char = ;code = 32
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char = s;code = 115
char = p;code = 112
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = y;code = 121
char = .;code = 46
char = ;code = 32
char = P;code = 80
char = a;code = 97
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = h;code = 104
char = a;code = 97
char = r;code = 114
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = z;code = 122
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = s;code = 115
char = p;code = 112
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = s;code = 115
char = c;code = 99
char = o;code = 111
char = p;code = 112
char = i;code = 105
char = c;code = 99
char = ;code = 32
char = t;code = 116
char = e;code = 101
char = c;code = 99
char = h;code = 104
char = n;code = 110
char = i;code = 105
char = q;code = 113
char = u;code = 117
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = p;code = 112
char = h;code = 104
char = y;code = 121
char = s;code = 115
char = i;code = 105
char = c;code = 99
char = o;code = 111
char = c;code = 99
char = h;code = 104
char = e;code = 101
char = m;code = 109
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = p;code = 112
char = a;code = 97
char = r;code = 114
char = a;code = 97
char = m;code = 109
char = e;code = 101
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = s;code = 115
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char = c;code = 99
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char = f;code = 102
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char = ;code = 32
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char = o;code = 111
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char = s;code = 115
char = l;code = 108
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char = n;code = 110
char = k;code = 107
char = i;code = 105
char = n;code = 110
char = g;code = 103
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char = b;code = 98
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char = f;code = 102
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char = a;code = 97
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char = ;code = 32
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char = N;code = 78
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char = b;code = 98
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char = l;code = 108
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = e;code = 101
char = n;code = 110
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char = e;code = 101
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char = c;code = 99
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char = i;code = 105
char = r;code = 114
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char = o;code = 111
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char = c;code = 99
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char = d;code = 100
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char = a;code = 97
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char = l;code = 108
char = i;code = 105
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char = y;code = 121
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char = t;code = 116
char = o;code = 111
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char = d;code = 100
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char = l;code = 108
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char = v;code = 118
char = e;code = 101
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char = e;code = 101
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char = l;code = 108
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = A;code = 65
char = m;code = 109
char = o;code = 111
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = v;code = 118
char = a;code = 97
char = r;code = 114
char = i;code = 105
char = o;code = 111
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char = a;code = 97
char = n;code = 110
char = o;code = 111
char = p;code = 112
char = a;code = 97
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char = i;code = 105
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char = ,;code = 44
char = ;code = 32
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char = A;code = 65
char = P;code = 80
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char = e;code = 101
char = /;code = 47
char = D;code = 68
char = N;code = 78
char = A;code = 65
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = m;code = 109
char = p;code = 112
char = l;code = 108
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char = e;code = 101
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char = l;code = 108
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char = s;code = 115
char = m;code = 109
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char = t;code = 116
char = o;code = 111
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char = t;code = 116
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char = e;code = 101
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char = l;code = 108
char = e;code = 101
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char = ;code = 32
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char = n;code = 110
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char = s;code = 115
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char = e;code = 101
char = l;code = 108
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = r;code = 114
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = (;code = 40
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char = 9;code = 57
char = );code = 41
char = T;code = 84
char = c;code = 99
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char = l;code = 108
char = a;code = 97
char = b;code = 98
char = e;code = 101
char = l;code = 108
char = e;code = 101
char = d;code = 100
char = ;code = 32
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char = A;code = 65
char = P;code = 80
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char = 2;code = 50
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char = N;code = 78
char = A;code = 65
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char = p;code = 112
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char = o;code = 111
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char = e;code = 101
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char = e;code = 101
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char = a;code = 97
char = l;code = 108
char = b;code = 98
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char = i;code = 105
char = c;code = 99
char = e;code = 101
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char = e;code = 101
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char = e;code = 101
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char = r;code = 114
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char = s;code = 115
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = m;code = 109
char = p;code = 112
char = l;code = 108
char = e;code = 101
char = x;code = 120
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = m;code = 109
char = o;code = 111
char = s;code = 115
char = t;code = 116
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char = o;code = 111
char = f;code = 102
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = n;code = 110
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char = t;code = 116
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char = ;code = 32
char = t;code = 116
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char = h;code = 104
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char = r;code = 114
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char = t;code = 116
char = e;code = 101
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char = t;code = 116
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char = o;code = 111
char = n;code = 110
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char = i;code = 105
char = n;code = 110
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char = i;code = 105
char = v;code = 118
char = e;code = 101
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char = ;code = 32
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char = n;code = 110
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char = n;code = 110
char = c;code = 99
char = l;code = 108
char = u;code = 117
char = s;code = 115
char = i;code = 105
char = o;code = 111
char = n;code = 110
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char = ;code = 32
char = H;code = 72
char = P;code = 80
char = P;code = 80
char = ,;code = 44
char = ;code = 32
char = A;code = 65
char = A;code = 65
char = P;code = 80
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = B;code = 66
char = A;code = 65
char = P;code = 80
char = ;code = 32
char = n;code = 110
char = a;code = 97
char = n;code = 110
char = o;code = 111
char = p;code = 112
char = a;code = 97
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = a;code = 97
char = f;code = 102
char = e;code = 101
char = ;code = 32
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = ;code = 32
char = e;code = 101
char = f;code = 102
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = i;code = 105
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = g;code = 103
char = e;code = 101
char = n;code = 110
char = e;code = 101
char = ;code = 32
char = d;code = 100
char = e;code = 101
char = l;code = 108
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = r;code = 114
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result sql = Polyethylenimine (PEI), a widely used cationic polymeric vector with high transfection efficiency, was converted into nanoparticles by introducing ionic and covalent crosslinkers with varying proportion of 1,6-hexanebisphosphate (HP), adipic acid (AA) and 1,4-butane dialdehyde (BA) to obtain a small library of HP-PEI (HPP), AA-PEI (AAP) and BA-PEI (BAP) nanoparticles, respectively. Particles were characterized by spectroscopic technique as well as physicochemical parameters such as size, morphology, surface charge, effect of crosslinking on buffering capacity and DNA binding ability. The entire series of nanoparticles were compared for their cytoxicity and ability to deliver genes in various cell lines. Among various nanoparticles, AAP-3 nanoparticle/DNA complex exhibited higher transfection efficiency (1.5-7.8 folds) than the native PEI (25kDa) and commercially available transfection reagents, such as GenePorter, GenePorter2, Fugene and Superfect, with cell viability >85%. The highest cell viability was observed with BAP nanoparticles (>95%). Importantly, the transfection activity of nanoparticle/DNA complexes was preserved in the presence of serum. Transfection with GFP-siRNA inhibited expression of transfected GFP gene by approximately 81-92%. All nanoparticle types (HPP, AAP and BAP) required a comparable time for entry into cells and subsequent intracellular passage from the cytoplasm to the nucleus. Intravenous delivery of (99)Tc labeled BAP-2/DNA complex to female Balb/c mice revealed the presence of the complex in most of the organs with the highest retention in liver. In conclusion, HPP, AAP and BAP nanoparticles are safe for efficient gene delivery.
Publication: Int J Pharm 2009 Jun 5 ;374(1-2)::125-38. :doi: 10.1016/j.ijpharm.2009.03.009.
Publication Date: 2009 Jun 5
Study Author(s): Swami, Archana;Goyal, Ritu;Tripathi, Sushil Kumar;Singh, Naresh;Katiyar, Neeraj;Mishra, Anil K;Gupta, K C;
Institution: Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi, India.
Shortcut link to this study:
Publication Date: 2009 Jun 5
Study Author(s): Swami, Archana;Goyal, Ritu;Tripathi, Sushil Kumar;Singh, Naresh;Katiyar, Neeraj;Mishra, Anil K;Gupta, K C;
Institution: Institute of Genomics and Integrative Biology, Delhi University Campus, Mall Road, Delhi, India.
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Polyethylenimine (PEI), a widely used cationic polymeric vector with high transfection efficiency, was converted into nanoparticles by introducing ionic and covalent crosslinkers with varying proportion of 1,6-hexanebisphosphate (HP), adipic acid (AA) and 1,4-butane dialdehyde (BA) to obtain a small library of HP-PEI (HPP), AA-PEI (AAP) and BA-PEI (BAP) nanoparticles, respectively. Particles were characterized by spectroscopic technique as well as physicochemical parameters such as size, morphology, surface charge, effect of crosslinking on buffering capacity and DNA binding ability. The entire series of nanoparticles were compared for their cytoxicity and ability to deliver genes in various cell lines. Among various nanoparticles, AAP-3 nanoparticle/DNA complex exhibited higher transfection efficiency (1.5-7.8 folds) than the native PEI (25kDa) and commercially available transfection reagents, such as GenePorter, GenePorter2, Fugene and Superfect, with cell viability >85%. The highest cell viability was observed with BAP nanoparticles (>95%). Importantly, the transfection activity of nanoparticle/DNA complexes was preserved in the presence of serum. Transfection with GFP-siRNA inhibited expression of transfected GFP gene by approximately 81-92%. All nanoparticle types (HPP, AAP and BAP) required a comparable time for entry into cells and subsequent intracellular passage from the cytoplasm to the nucleus. Intravenous delivery of (99)Tc labeled BAP-2/DNA complex to female Balb/c mice revealed the presence of the complex in most of the organs with the highest retention in liver. In conclusion, HPP, AAP and BAP nanoparticles are safe for efficient gene delivery.
PMID: 19446769
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