char = U;code = 85 char = p;code = 112 char = t;code = 116 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = .;code = 46 result sql = Uptake of ferrous iron by cultured rat astrocytes.
Uptake of ferrous iron by cultured rat astrocytes.char = A;code = 65 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = i;code = 105 char = d;code = 100 char = e;code = 101 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = p;code = 112 char = l;code = 108 char = a;code = 97 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = m;code = 109 char = p;code = 112 char = o;code = 111 char = r;code = 114 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = r;code = 114 char = o;code = 111 char = l;code = 108 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = h;code = 104 char = o;code = 111 char = m;code = 109 char = e;code = 101 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = y;code = 121 char = e;code = 101 char = t;code = 116 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = o;code = 111 char = l;code = 108 char = v;code = 118 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = m;code = 109 char = a;code = 97 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = l;code = 108 char = u;code = 117 char = s;code = 115 char = i;code = 105 char = v;code = 118 char = e;code = 101 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = o;code = 111 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = v;code = 118 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = i;code = 105 char = g;code = 103 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = e;code = 101 char = ;code = 32 char = h;code = 104 char = a;code = 97 char = v;code = 118 char = e;code = 101 char = ;code = 32 char = a;code = 97 char = p;code = 112 char = p;code = 112 char = l;code = 108 char = i;code = 105 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = m;code = 109 char = m;code = 109 char = o;code = 111 char = n;code = 110 char = i;code = 105 char = u;code = 117 char = m;code = 109 char = ;code = 32 char = c;code = 99 char = i;code = 105 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = (;code = 40 char = F;code = 70 char = A;code = 65 char = C;code = 67 char = );code = 41 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = -;code = 45 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = i;code = 105 char = m;code = 109 char = a;code = 97 char = r;code = 114 char = y;code = 121 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = c;code = 99 char = u;code = 117 char = l;code = 108 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = m;code = 109 char = R;code = 82 char = N;code = 78 char = A;code = 65 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = t;code = 116 char = w;code = 119 char = o;code = 111 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = m;code = 109 char = b;code = 98 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = e;code = 101 char = -;code = 45 char = b;code = 98 char = o;code = 111 char = u;code = 117 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = D;code = 68 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = b;code = 98 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = S;code = 83 char = D;code = 68 char = R;code = 82 char = 2;code = 50 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = (;code = 40 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = M;code = 77 char = );code = 41 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = 3;code = 51 char = .;code = 46 char = 2;code = 50 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 0;code = 48 char = .;code = 46 char = 4;code = 52 char = ;code = 32 char = n;code = 110 char = m;code = 109 char = o;code = 111 char = l;code = 108 char = /;code = 47 char = (;code = 40 char = h;code = 104 char = r;code = 114 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = m;code = 109 char = g;code = 103 char = );code = 41 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = b;code = 98 char = s;code = 115 char = t;code = 116 char = a;code = 97 char = n;code = 110 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = M;code = 77 char = ;code = 32 char = F;code = 70 char = A;code = 65 char = C;code = 67 char = ;code = 32 char = [;code = 91 char = 2;code = 50 char = 4;code = 52 char = .;code = 46 char = 7;code = 55 char = ;code = 32 char = +;code = 43 char = /;code = 47 char = -;code = 45 char = ;code = 32 char = 8;code = 56 char = .;code = 46 char = 9;code = 57 char = ;code = 32 char = n;code = 110 char = m;code = 109 char = o;code = 111 char = l;code = 108 char = /;code = 47 char = (;code = 40 char = h;code = 104 char = r;code = 114 char = ;code = 32 char = x;code = 120 char = ;code = 32 char = m;code = 109 char = g;code = 103 char = );code = 41 char = ];code = 93 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = i;code = 105 char = c;code = 99 char = h;code = 104 char = ;code = 32 char = s;code = 115 char = u;code = 117 char = g;code = 103 char = g;code = 103 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = F;code = 70 char = A;code = 65 char = C;code = 67 char = ;code = 32 char = d;code = 100 char = o;code = 111 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = b;code = 98 char = e;code = 101 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = p;code = 112 char = a;code = 97 char = r;code = 114 char = t;code = 116 char = i;code = 105 char = a;code = 97 char = l;code = 108 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = c;code = 99 char = e;code = 101 char = l;code = 108 char = l;code = 108 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = r;code = 114 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = N;code = 78 char = o;code = 111 char = n;code = 110 char = e;code = 101 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = w;code = 119 char = h;code = 104 char = e;code = 101 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = F;code = 70 char = A;code = 65 char = C;code = 67 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = l;code = 108 char = m;code = 109 char = o;code = 111 char = s;code = 115 char = t;code = 116 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = m;code = 109 char = p;code = 112 char = l;code = 108 char = e;code = 101 char = t;code = 116 char = e;code = 101 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = r;code = 114 char = e;code = 101 char = d;code = 100 char = u;code = 117 char = c;code = 99 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = c;code = 99 char = e;code = 101 char = s;code = 115 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = e;code = 101 char = x;code = 120 char = o;code = 111 char = g;code = 103 char = e;code = 101 char = n;code = 110 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = c;code = 99 char = o;code = 111 char = r;code = 114 char = b;code = 98 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = ;code = 32 char = t;code = 116 char = i;code = 105 char = m;code = 109 char = e;code = 101 char = -;code = 45 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = -;code = 45 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = m;code = 109 char = a;code = 97 char = n;code = 110 char = n;code = 110 char = e;code = 101 char = r;code = 114 char = ;code = 32 char = w;code = 119 char = i;code = 105 char = t;code = 116 char = h;code = 104 char = ;code = 32 char = s;code = 115 char = p;code = 112 char = e;code = 101 char = c;code = 99 char = i;code = 105 char = f;code = 102 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = r;code = 114 char = e;code = 101 char = a;code = 97 char = s;code = 115 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = l;code = 108 char = i;code = 105 char = n;code = 110 char = e;code = 101 char = a;code = 97 char = r;code = 114 char = l;code = 108 char = y;code = 121 char = ;code = 32 char = f;code = 102 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = c;code = 99 char = o;code = 111 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = s;code = 115 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = 1;code = 49 char = 0;code = 48 char = 0;code = 48 char = ;code = 32 char = m;code = 109 char = u;code = 117 char = M;code = 77 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = i;code = 105 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = w;code = 119 char = a;code = 97 char = s;code = 115 char = ;code = 32 char = a;code = 97 char = t;code = 116 char = t;code = 116 char = e;code = 101 char = n;code = 110 char = u;code = 117 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = c;code = 99 char = u;code = 117 char = b;code = 98 char = a;code = 97 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = e;code = 101 char = m;code = 109 char = p;code = 112 char = e;code = 101 char = r;code = 114 char = a;code = 97 char = t;code = 116 char = u;code = 117 char = r;code = 114 char = e;code = 101 char = ,;code = 44 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = r;code = 114 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = n;code = 110 char = c;code = 99 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = c;code = 99 char = h;code = 104 char = e;code = 101 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = o;code = 111 char = r;code = 114 char = s;code = 115 char = ,;code = 44 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = l;code = 108 char = o;code = 111 char = w;code = 119 char = e;code = 101 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = g;code = 103 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = p;code = 112 char = H;code = 72 char = ;code = 32 char = f;code = 102 char = r;code = 114 char = o;code = 111 char = m;code = 109 char = ;code = 32 char = 7;code = 55 char = .;code = 46 char = 4;code = 52 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = 6;code = 54 char = .;code = 46 char = 8;code = 56 char = .;code = 46 char = ;code = 32 char = T;code = 84 char = h;code = 104 char = e;code = 101 char = s;code = 115 char = e;code = 101 char = ;code = 32 char = d;code = 100 char = a;code = 97 char = t;code = 116 char = a;code = 97 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = i;code = 105 char = c;code = 99 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ,;code = 44 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = d;code = 100 char = d;code = 100 char = i;code = 105 char = t;code = 116 char = i;code = 105 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = t;code = 116 char = o;code = 111 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = e;code = 101 char = ;code = 32 char = D;code = 68 char = M;code = 77 char = T;code = 84 char = 1;code = 49 char = -;code = 45 char = m;code = 109 char = e;code = 101 char = d;code = 100 char = i;code = 105 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = d;code = 100 char = ;code = 32 char = u;code = 117 char = p;code = 112 char = t;code = 116 char = a;code = 97 char = k;code = 107 char = e;code = 101 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ,;code = 44 char = ;code = 32 char = a;code = 97 char = s;code = 115 char = t;code = 116 char = r;code = 114 char = o;code = 111 char = c;code = 99 char = y;code = 121 char = t;code = 116 char = e;code = 101 char = s;code = 115 char = ;code = 32 char = c;code = 99 char = a;code = 97 char = n;code = 110 char = ;code = 32 char = a;code = 97 char = c;code = 99 char = c;code = 99 char = u;code = 117 char = m;code = 109 char = u;code = 117 char = l;code = 108 char = a;code = 97 char = t;code = 116 char = e;code = 101 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = c;code = 99 char = ;code = 32 char = a;code = 97 char = n;code = 110 char = d;code = 100 char = ;code = 32 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = o;code = 111 char = u;code = 117 char = s;code = 115 char = ;code = 32 char = i;code = 105 char = r;code = 114 char = o;code = 111 char = n;code = 110 char = ;code = 32 char = b;code = 98 char = y;code = 121 char = ;code = 32 char = m;code = 109 char = e;code = 101 char = c;code = 99 char = h;code = 104 char = a;code = 97 char = n;code = 110 char = i;code = 105 char = s;code = 115 char = m;code = 109 char = s;code = 115 char = ;code = 32 char = t;code = 116 char = h;code = 104 char = a;code = 97 char = t;code = 116 char = ;code = 32 char = a;code = 97 char = r;code = 114 char = e;code = 101 char = ;code = 32 char = i;code = 105 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = p;code = 112 char = e;code = 101 char = n;code = 110 char = d;code = 100 char = e;code = 101 char = n;code = 110 char = t;code = 116 char = ;code = 32 char = o;code = 111 char = f;code = 102 char = ;code = 32 char = D;code = 68 char = M;code = 77 char = T;code = 84 char = 1;code = 49 char = ;code = 32 char = o;code = 111 char = r;code = 114 char = ;code = 32 char = t;code = 116 char = r;code = 114 char = a;code = 97 char = n;code = 110 char = s;code = 115 char = f;code = 102 char = e;code = 101 char = r;code = 114 char = r;code = 114 char = i;code = 105 char = n;code = 110 char = .;code = 46 char = <;code = 60 char = b;code = 98 char = r;code = 114 char = /;code = 47 char = >;code = 62 result sql = Astrocytes are considered to play an important role in iron homeostasis of the brain, yet the mechanisms involved in the uptake of iron into astrocytes remain elusive. To investigate the uptake of iron into astrocytes, we have applied ferric ammonium citrate (FAC) to rat astrocyte-rich primary cultures. These cultures express the mRNAs of two membrane-bound ferric reductases, Dcytb and SDR2, and reduce extracellular ferric iron (100 muM) with a rate of 3.2 +/- 0.4 nmol/(hr x mg). This reduction rate is substantially lower than the rate of cellular iron accumulation from 100 muM FAC [24.7 +/- 8.9 nmol/(hr x mg)], which suggests that iron accumulation from FAC does at best partially depend on extracellular ferric reduction. Nonetheless, when the iron in FAC was almost completely reduced by an excess of exogenous ascorbate, astrocytes accumulated iron in a time- and concentration-dependent manner with specific iron accumulation rates that increased linearly for concentrations of up to 100 muM ferrous iron. This accumulation was attenuated by lowering the incubation temperature, by the presence of ferrous iron chelators, or by lowering the pH from 7.4 to 6.8. These data indicate that, in addition to the DMT1-mediated uptake of ferrous iron, astrocytes can accumulate ferric and ferrous iron by mechanisms that are independent of DMT1 or transferrin.
Publication: J. Neurosci. Res. 2010 Feb 15 ;88(3)::563-71. :doi: 10.1002/jnr.22217.
Publication Date: 2010 Feb 15
Study Author(s): Tulpule, Ketki;Robinson, Stephen R;Bishop, Glenda M;Dringen, Ralf;
Institution: Center for Biomolecular Interactions Bremen, University of Bremen, Bremen, Germany.
Shortcut link to this study:
Astrocytes are considered to play an important role in iron homeostasis of the brain, yet the mechanisms involved in the uptake of iron into astrocytes remain elusive. To investigate the uptake of iron into astrocytes, we have applied ferric ammonium citrate (FAC) to rat astrocyte-rich primary cultures. These cultures express the mRNAs of two membrane-bound ferric reductases, Dcytb and SDR2, and reduce extracellular ferric iron (100 muM) with a rate of 3.2 +/- 0.4 nmol/(hr x mg). This reduction rate is substantially lower than the rate of cellular iron accumulation from 100 muM FAC [24.7 +/- 8.9 nmol/(hr x mg)], which suggests that iron accumulation from FAC does at best partially depend on extracellular ferric reduction. Nonetheless, when the iron in FAC was almost completely reduced by an excess of exogenous ascorbate, astrocytes accumulated iron in a time- and concentration-dependent manner with specific iron accumulation rates that increased linearly for concentrations of up to 100 muM ferrous iron. This accumulation was attenuated by lowering the incubation temperature, by the presence of ferrous iron chelators, or by lowering the pH from 7.4 to 6.8. These data indicate that, in addition to the DMT1-mediated uptake of ferrous iron, astrocytes can accumulate ferric and ferrous iron by mechanisms that are independent of DMT1 or transferrin.
PMID: 19746426