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char = s;code = 115
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char = J;code = 74
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char = a;code = 97
char = n;code = 110
char = .;code = 46
result sql = Microbial methane production in deep aquifer associated with the accretionary prism in Japan.
Microbial methane production in deep aquifer associated with the accretionary prism in Japan.
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char = s;code = 115
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char = w;code = 119
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char = ;code = 32
char = g;code = 103
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char = c;code = 99
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char = e;code = 101
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char = a;code = 97
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char = ;code = 32
char = a;code = 97
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char = c;code = 99
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char = i;code = 105
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char = o;code = 111
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char = i;code = 105
char = c;code = 99
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char = a;code = 97
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char = s;code = 115
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char = o;code = 111
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char = m;code = 109
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char = e;code = 101
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char = i;code = 105
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char = a;code = 97
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char = g;code = 103
char = a;code = 97
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char = d;code = 100
char = ;code = 32
char = d;code = 100
char = i;code = 105
char = s;code = 115
char = s;code = 115
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char = e;code = 101
char = d;code = 100
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char = i;code = 105
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char = o;code = 111
char = r;code = 114
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char = a;code = 97
char = n;code = 110
char = i;code = 105
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char = c;code = 99
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char = b;code = 98
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char = n;code = 110
char = ;code = 32
char = (;code = 40
char = m;code = 109
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = l;code = 108
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char = b;code = 98
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char = c;code = 99
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char = b;code = 98
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char = g;code = 103
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char = t;code = 116
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char = a;code = 97
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char = t;code = 116
char = h;code = 104
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char = m;code = 109
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char = h;code = 104
char = a;code = 97
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char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = d;code = 100
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char = r;code = 114
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char = e;code = 101
char = d;code = 100
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char = f;code = 102
char = r;code = 114
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char = m;code = 109
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char = t;code = 116
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char = s;code = 115
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char = s;code = 115
char = .;code = 46
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char = h;code = 104
char = a;code = 97
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char = a;code = 97
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char = 1;code = 49
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char = S;code = 83
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char = r;code = 114
char = R;code = 82
char = N;code = 78
char = A;code = 65
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char = g;code = 103
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char = g;code = 103
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char = H;code = 72
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char = d;code = 100
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char = C;code = 67
char = O;code = 79
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char = .;code = 46
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char = B;code = 66
char = a;code = 97
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char = S;code = 83
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char = A;code = 65
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char = g;code = 103
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char = e;code = 101
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char = n;code = 110
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char = p;code = 112
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char = u;code = 117
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char = s;code = 115
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char = b;code = 98
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char = a;code = 97
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char = a;code = 97
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char = d;code = 100
char = ;code = 32
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char = (;code = 40
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char = s;code = 115
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char = n;code = 110
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char = h;code = 104
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char = s;code = 115
char = ;code = 32
char = a;code = 97
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char = t;code = 116
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char = r;code = 114
char = m;code = 109
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char = f;code = 102
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char = a;code = 97
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char = s;code = 115
char = o;code = 111
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char = a;code = 97
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char = e;code = 101
char = d;code = 100
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char = h;code = 104
char = ;code = 32
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char = o;code = 111
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char = r;code = 114
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char = s;code = 115
char = m;code = 109
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char = n;code = 110
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result sql = To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H(2)-using methanogens in the groundwater. Furthermore, the high potential of methane production by H(2)-using methanogens was shown in enrichments using groundwater amended with H(2) and CO(2). Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H(2) and CO(2) generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H(2)-producing fermentative bacteria and H(2)-using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H(2) accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 degrees C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H(2)-producing fermentative bacteria and H(2)-using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.

Publication: ISME J 2010 Apr ;4(4)::531-41.
Publication Date: 2010 Apr
Study Author(s): Kimura, Hiroyuki;Nashimoto, Hiroaki;Shimizu, Mikio;Hattori, Shohei;Yamada, Keita;Koba, Keisuke;Yoshida, Naohiro;Kato, Kenji;
Institution: Department of Geosciences, Faculty of Science, Shizuoka University, Shizuoka, Japan. [email protected]
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To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H(2)-using methanogens in the groundwater. Furthermore, the high potential of methane production by H(2)-using methanogens was shown in enrichments using groundwater amended with H(2) and CO(2). Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H(2) and CO(2) generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H(2)-producing fermentative bacteria and H(2)-using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H(2) accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 degrees C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H(2)-producing fermentative bacteria and H(2)-using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.
PMID: 19956275

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