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result sql = Evaluation of surface sampling techniques for collection of Bacillus spores on common drinking water pipe materials.
Evaluation of surface sampling techniques for collection of Bacillus spores on common drinking water pipe materials.
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char = r;code = 114
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char = s;code = 115
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char = ;code = 32
char = +;code = 43
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char = e;code = 101
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char = e;code = 101
char = s;code = 115
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char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = s;code = 115
char = e;code = 101
char = e;code = 101
char = n;code = 110
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = t;code = 116
char = w;code = 119
char = e;code = 101
char = e;code = 101
char = n;code = 110
char = ;code = 32
char = b;code = 98
char = r;code = 114
char = u;code = 117
char = s;code = 115
char = h;code = 104
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = s;code = 115
char = c;code = 99
char = r;code = 114
char = a;code = 97
char = p;code = 112
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = P;code = 80
char = V;code = 86
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char = r;code = 114
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char = r;code = 114
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char = h;code = 104
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = s;code = 115
char = c;code = 99
char = r;code = 114
char = a;code = 97
char = p;code = 112
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = c;code = 99
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char = e;code = 101
char = r;code = 114
char = i;code = 105
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char = ;code = 32
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char = r;code = 114
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char = ;code = 32
char = P;code = 80
char = V;code = 86
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char = ;code = 32
char = c;code = 99
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char = p;code = 112
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = ;code = 32
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char = e;code = 101
char = r;code = 114
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char = o;code = 111
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char = a;code = 97
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char = e;code = 101
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char = p;code = 112
char = o;code = 111
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char = ;code = 32
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char = p;code = 112
char = o;code = 111
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char = e;code = 101
char = ;code = 32
char = r;code = 114
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char = e;code = 101
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char = t;code = 116
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char = o;code = 111
char = n;code = 110
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char = i;code = 105
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = d;code = 100
char = ;code = 32
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char = e;code = 101
char = t;code = 116
char = w;code = 119
char = e;code = 101
char = e;code = 101
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char = ;code = 32
char = p;code = 112
char = i;code = 105
char = p;code = 112
char = e;code = 101
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = s;code = 115
char = ;code = 32
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char = d;code = 100
char = ;code = 32
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char = e;code = 101
char = ;code = 32
char = p;code = 112
char = r;code = 114
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char = s;code = 115
char = e;code = 101
char = n;code = 110
char = c;code = 99
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = a;code = 97
char = b;code = 98
char = l;code = 108
char = i;code = 105
char = s;code = 115
char = h;code = 104
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = i;code = 105
char = o;code = 111
char = f;code = 102
char = i;code = 105
char = l;code = 108
char = m;code = 109
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = l;code = 108
char = s;code = 115
char = o;code = 111
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = m;code = 109
char = p;code = 112
char = a;code = 97
char = c;code = 99
char = t;code = 116
char = .;code = 46
char = ;code = 32
char = C;code = 67
char = o;code = 111
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char = d;code = 100
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char = i;code = 105
char = o;code = 111
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char = d;code = 100
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char = P;code = 80
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char = ;code = 32
char = c;code = 99
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char = p;code = 112
char = o;code = 111
char = n;code = 110
char = s;code = 115
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char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = e;code = 101
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char = t;code = 116
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char = b;code = 98
char = l;code = 108
char = i;code = 105
char = s;code = 115
char = h;code = 104
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = i;code = 105
char = o;code = 111
char = f;code = 102
char = i;code = 105
char = l;code = 108
char = m;code = 109
char = s;code = 115
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char = ;code = 32
char = h;code = 104
char = a;code = 97
char = d;code = 100
char = ;code = 32
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char = i;code = 105
char = g;code = 103
char = n;code = 110
char = i;code = 105
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = a;code = 97
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char = t;code = 116
char = l;code = 108
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char = l;code = 108
char = o;code = 111
char = w;code = 119
char = e;code = 101
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char = ;code = 32
char = s;code = 115
char = p;code = 112
char = o;code = 111
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = r;code = 114
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char = e;code = 101
char = n;code = 110
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char = o;code = 111
char = n;code = 110
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char = ;code = 32
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char = -;code = 45
char = ;code = 32
char = 1;code = 49
char = 1;code = 49
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char = ;code = 32
char = t;code = 116
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char = n;code = 110
char = ;code = 32
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char = n;code = 110
char = d;code = 100
char = i;code = 105
char = t;code = 116
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char = o;code = 111
char = n;code = 110
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char = d;code = 100
char = ;code = 32
char = c;code = 99
char = e;code = 101
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char = e;code = 101
char = n;code = 110
char = t;code = 116
char = -;code = 45
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char = i;code = 105
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 6;code = 54
char = 1;code = 49
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 1;code = 49
char = 4;code = 52
char = %;code = 37
char = );code = 41
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = n;code = 110
char = d;code = 100
char = i;code = 105
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = i;code = 105
char = r;code = 114
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = u;code = 117
char = p;code = 112
char = o;code = 111
char = n;code = 110
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = 7;code = 55
char = 1;code = 49
char = ;code = 32
char = +;code = 43
char = /;code = 47
char = -;code = 45
char = ;code = 32
char = 8;code = 56
char = %;code = 37
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char = .;code = 46
char = <;code = 60
char = b;code = 98
char = r;code = 114
char = /;code = 47
char = >;code = 62
result sql = Drinking water utilities may face biological contamination of the distribution system from a natural incident or deliberate contamination. Determining the extent of contamination or the efficacy of decontamination is a challenge, because it may require sampling of the wetted surfaces of distribution infrastructure. This study evaluated two sampling techniques that utilities might use to sample exhumed pipe sections. Polyvinyl chloride (PVC), cement-lined ductile iron, and ductile iron pipe coupons (3 cm x 14 cm) cut from new water main piping were conditioned for three months in dechlorinated Cincinnati, Ohio tap water. Coupons were spiked with Bacillus atrophaeus subsp. globigii, a surrogate for Bacillus anthracis. Brushing and scraping were used to recover the inoculated spores from the coupons. Mean recoveries for all materials ranged from 37 +/- 30% to 43 +/- 20% for brushing vs. 24 +/- 10% to 51 +/- 29% for scraping. On cement-lined pipe, brushing yielded a significantly different recovery than scraping. No differences were seen between brushing and scraping the PVC and iron pipe coupons. Mean brushing and scraping recoveries from PVC coupons were more variable than mean recoveries from cement-lined and iron coupons. Spore retention differed between pipe materials and the presence of established biofilms also had an impact. Conditioned PVC coupons (with established biofilms) had significantly lower spore retention (31 +/- 11%) than conditioned cement-lined coupons (61 +/- 14%) and conditioned iron coupons (71 +/- 8%).

Publication: J Environ Monit 2010 Jan ;12(1)::361-8. :doi: 10.1039/b917570a.
Publication Date: 2010 Jan
Study Author(s): Packard, Benjamin H;Kupferle, Margaret J;
Institution: National Homeland Security Research Center, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, OH 45268, USA. [email protected]
Shortcut link to this study:
Drinking water utilities may face biological contamination of the distribution system from a natural incident or deliberate contamination. Determining the extent of contamination or the efficacy of decontamination is a challenge, because it may require sampling of the wetted surfaces of distribution infrastructure. This study evaluated two sampling techniques that utilities might use to sample exhumed pipe sections. Polyvinyl chloride (PVC), cement-lined ductile iron, and ductile iron pipe coupons (3 cm x 14 cm) cut from new water main piping were conditioned for three months in dechlorinated Cincinnati, Ohio tap water. Coupons were spiked with Bacillus atrophaeus subsp. globigii, a surrogate for Bacillus anthracis. Brushing and scraping were used to recover the inoculated spores from the coupons. Mean recoveries for all materials ranged from 37 +/- 30% to 43 +/- 20% for brushing vs. 24 +/- 10% to 51 +/- 29% for scraping. On cement-lined pipe, brushing yielded a significantly different recovery than scraping. No differences were seen between brushing and scraping the PVC and iron pipe coupons. Mean brushing and scraping recoveries from PVC coupons were more variable than mean recoveries from cement-lined and iron coupons. Spore retention differed between pipe materials and the presence of established biofilms also had an impact. Conditioned PVC coupons (with established biofilms) had significantly lower spore retention (31 +/- 11%) than conditioned cement-lined coupons (61 +/- 14%) and conditioned iron coupons (71 +/- 8%).
PMID: 20082033

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