|
|
char = C;code = 67
char = y;code = 121
char = c;code = 99
char = l;code = 108
char = o;code = 111
char = d;code = 100
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = n;code = 110
char = ;code = 32
char = i;code = 105
char = n;code = 110
char = d;code = 100
char = u;code = 117
char = c;code = 99
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = c;code = 99
char = i;code = 105
char = u;code = 117
char = m;code = 109
char = -;code = 45
char = d;code = 100
char = e;code = 101
char = p;code = 112
char = e;code = 101
char = n;code = 110
char = d;code = 100
char = e;code = 101
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char = t;code = 116
char = ;code = 32
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char = y;code = 121
char = s;code = 115
char = o;code = 111
char = s;code = 115
char = o;code = 111
char = m;code = 109
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = e;code = 101
char = x;code = 120
char = o;code = 111
char = c;code = 99
char = y;code = 121
char = t;code = 116
char = o;code = 111
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = .;code = 46
result sql = Cyclodextrin induces calcium-dependent lysosomal exocytosis.
Cyclodextrin induces calcium-dependent lysosomal exocytosis.char = C;code = 67
char = y;code = 121
char = c;code = 99
char = l;code = 108
char = o;code = 111
char = d;code = 100
char = e;code = 101
char = x;code = 120
char = t;code = 116
char = r;code = 114
char = i;code = 105
char = n;code = 110
char = s;code = 115
char = ;code = 32
char = (;code = 40
char = C;code = 67
char = D;code = 68
char = s;code = 115
char = );code = 41
char = ;code = 32
char = h;code = 104
char = a;code = 97
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = l;code = 108
char = o;code = 111
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = b;code = 98
char = e;code = 101
char = e;code = 101
char = n;code = 110
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char = u;code = 117
char = s;code = 115
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = n;code = 110
char = i;code = 105
char = p;code = 112
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = e;code = 101
char = l;code = 108
char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
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char = c;code = 99
char = h;code = 104
char = o;code = 111
char = l;code = 108
char = e;code = 101
char = s;code = 115
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = o;code = 111
char = l;code = 108
char = ;code = 32
char = l;code = 108
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = s;code = 115
char = ;code = 32
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char = o;code = 111
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char = h;code = 104
char = ;code = 32
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char = v;code = 118
char = i;code = 105
char = t;code = 116
char = r;code = 114
char = o;code = 111
char = ;code = 32
char = a;code = 97
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char = d;code = 100
char = ;code = 32
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char = n;code = 110
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char = v;code = 118
char = i;code = 105
char = v;code = 118
char = o;code = 111
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char = ;code = 32
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char = t;code = 116
char = ;code = 32
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char = h;code = 104
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char = ;code = 32
char = d;code = 100
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char = c;code = 99
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char = ;code = 32
char = e;code = 101
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = c;code = 99
char = e;code = 101
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char = l;code = 108
char = u;code = 117
char = l;code = 108
char = a;code = 97
char = r;code = 114
char = ;code = 32
char = l;code = 108
char = e;code = 101
char = v;code = 118
char = e;code = 101
char = l;code = 108
char = ;code = 32
char = a;code = 97
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = n;code = 110
char = o;code = 111
char = t;code = 116
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = l;code = 108
char = l;code = 108
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char = c;code = 99
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char = a;code = 97
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char = a;code = 97
char = c;code = 99
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char = z;code = 122
char = e;code = 101
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char = R;code = 82
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char = c;code = 99
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = l;code = 108
char = y;code = 121
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char = ;code = 32
char = C;code = 67
char = D;code = 68
char = s;code = 115
char = ;code = 32
char = h;code = 104
char = a;code = 97
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char = e;code = 101
char = ;code = 32
char = g;code = 103
char = a;code = 97
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char = n;code = 110
char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = m;code = 109
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char = c;code = 99
char = h;code = 104
char = ;code = 32
char = i;code = 105
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char = t;code = 116
char = e;code = 101
char = r;code = 114
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char = s;code = 115
char = t;code = 116
char = ;code = 32
char = b;code = 98
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char = c;code = 99
char = a;code = 97
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char = o;code = 111
char = f;code = 102
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char = a;code = 97
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char = e;code = 101
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char = o;code = 111
char = l;code = 108
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char = f;code = 102
char = r;code = 114
char = o;code = 111
char = m;code = 109
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char = N;code = 78
char = i;code = 105
char = e;code = 101
char = m;code = 109
char = a;code = 97
char = n;code = 110
char = n;code = 110
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char = P;code = 80
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char = c;code = 99
char = k;code = 107
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char = T;code = 84
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char = ;code = 32
char = C;code = 67
char = ;code = 32
char = (;code = 40
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char = C;code = 67
char = );code = 41
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char = c;code = 99
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char = m;code = 109
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char = k;code = 107
char = e;code = 101
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char = l;code = 108
char = y;code = 121
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char = p;code = 112
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char = o;code = 111
char = l;code = 108
char = o;code = 111
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char = g;code = 103
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
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char = l;code = 108
char = i;code = 105
char = f;code = 102
char = e;code = 101
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = N;code = 78
char = P;code = 80
char = C;code = 67
char = 1;code = 49
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char = H;code = 72
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char = t;code = 116
char = h;code = 104
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char = h;code = 104
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char = o;code = 111
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char = e;code = 101
char = s;code = 115
char = i;code = 105
char = s;code = 115
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = a;code = 97
char = t;code = 116
char = ;code = 32
char = t;code = 116
char = r;code = 114
char = e;code = 101
char = a;code = 97
char = t;code = 116
char = m;code = 109
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = 2;code = 50
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char = d;code = 100
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char = x;code = 120
char = y;code = 121
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char = r;code = 114
char = o;code = 111
char = p;code = 112
char = y;code = 121
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char = #;code = 35
char = 9;code = 57
char = 4;code = 52
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char = c;code = 99
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char = c;code = 99
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char = d;code = 100
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char = t;code = 116
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char = i;code = 105
char = n;code = 110
char = ;code = 32
char = (;code = 40
char = H;code = 72
char = P;code = 80
char = B;code = 66
char = -;code = 45
char = C;code = 67
char = D;code = 68
char = );code = 41
char = ;code = 32
char = s;code = 115
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char = m;code = 109
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char = l;code = 108
char = a;code = 97
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char = e;code = 101
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char = c;code = 99
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char = n;code = 110
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char = .;code = 46
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char = W;code = 87
char = e;code = 101
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char = ;code = 32
char = H;code = 72
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char = B;code = 66
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result sql = Cyclodextrins (CDs) have long been used to manipulate cellular cholesterol levels both in vitro and in vivo, but their direct effects at a cellular level are not well characterized. Recently, CDs have garnered much interest because of their ability to clear stored cholesterol from Niemann Pick Type C (NPC) cells and markedly prolong the life of NPC1 disease mice. Here, we investigate the hypothesis that treatment with 2-hydroxypropyl- β-cyclodextrin (HPB-CD) stimulates lysosomal exocytosis in a calcium-enhanced manner. We propose that this exocytosis is the mechanism by which HPB-CD ameliorates the endolysosomal cholesterol storage phenotype in NPC cells. These findings have significant implications for the use of HPB-CD in biochemical assays and data interpretation as well as for their use for the treatment for NPC and other disorders.
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result sql = Cyclodextrin induces calcium-dependent lysosomal exocytosis.
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char = d;code = 100
char = i;code = 105
char = s;code = 115
char = o;code = 111
char = r;code = 114
char = d;code = 100
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = .;code = 46
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char = b;code = 98
char = r;code = 114
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char = >;code = 62
result sql = Cyclodextrins (CDs) have long been used to manipulate cellular cholesterol levels both in vitro and in vivo, but their direct effects at a cellular level are not well characterized. Recently, CDs have garnered much interest because of their ability to clear stored cholesterol from Niemann Pick Type C (NPC) cells and markedly prolong the life of NPC1 disease mice. Here, we investigate the hypothesis that treatment with 2-hydroxypropyl- β-cyclodextrin (HPB-CD) stimulates lysosomal exocytosis in a calcium-enhanced manner. We propose that this exocytosis is the mechanism by which HPB-CD ameliorates the endolysosomal cholesterol storage phenotype in NPC cells. These findings have significant implications for the use of HPB-CD in biochemical assays and data interpretation as well as for their use for the treatment for NPC and other disorders.
Publication: PLoS ONE 2010 ;5(11)::e15054. :doi: 10.1371/journal.pone.0015054.
Publication Date: 2010
Study Author(s): Chen, Fannie W;Li, Chunlei;Ioannou, Yiannis A;
Institution: Department of Genetics and Genomic Sciences, The Mount Sinai School of Medicine, New York, New York, USA.
Shortcut link to this study:
Publication Date: 2010
Study Author(s): Chen, Fannie W;Li, Chunlei;Ioannou, Yiannis A;
Institution: Department of Genetics and Genomic Sciences, The Mount Sinai School of Medicine, New York, New York, USA.
Shortcut link to this study:
Cyclodextrins (CDs) have long been used to manipulate cellular cholesterol levels both in vitro and in vivo, but their direct effects at a cellular level are not well characterized. Recently, CDs have garnered much interest because of their ability to clear stored cholesterol from Niemann Pick Type C (NPC) cells and markedly prolong the life of NPC1 disease mice. Here, we investigate the hypothesis that treatment with 2-hydroxypropyl- β-cyclodextrin (HPB-CD) stimulates lysosomal exocytosis in a calcium-enhanced manner. We propose that this exocytosis is the mechanism by which HPB-CD ameliorates the endolysosomal cholesterol storage phenotype in NPC cells. These findings have significant implications for the use of HPB-CD in biochemical assays and data interpretation as well as for their use for the treatment for NPC and other disorders.
PMID: 21124786
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