char = G;code = 71
char = o;code = 111
char = l;code = 108
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char = ,;code = 44
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = r;code = 114
char = b;code = 98
char = o;code = 111
char = n;code = 110
char = ,;code = 44
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = l;code = 108
char = u;code = 117
char = m;code = 109
char = i;code = 105
char = n;code = 110
char = u;code = 117
char = m;code = 109
char = ;code = 32
char = l;code = 108
char = o;code = 111
char = w;code = 119
char = -;code = 45
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char = f;code = 102
char = l;code = 108
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
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char = o;code = 111
char = m;code = 109
char = p;code = 112
char = a;code = 97
char = c;code = 99
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char = ;code = 32
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char = i;code = 105
char = s;code = 115
char = c;code = 99
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = m;code = 109
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char = c;code = 99
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char = o;code = 111
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = a;code = 97
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char = n;code = 110
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char = p;code = 112
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char = a;code = 97
char = t;code = 116
char = f;code = 102
char = o;code = 111
char = r;code = 114
char = m;code = 109
char = s;code = 115
char = .;code = 46
result sql = Gold, carbon, and aluminum low-reflectivity compact discs as microassaying platforms.
Gold, carbon, and aluminum low-reflectivity compact discs as microassaying platforms.
char = C;code = 67
char = o;code = 111
char = m;code = 109
char = p;code = 112
char = a;code = 97
char = c;code = 99
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char = c;code = 99
char = ;code = 32
char = (;code = 40
char = C;code = 67
char = D;code = 68
char = );code = 41
char = ;code = 32
char = s;code = 115
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char = r;code = 114
char = f;code = 102
char = a;code = 97
char = c;code = 99
char = e;code = 101
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char = m;code = 109
char = o;code = 111
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char = i;code = 105
char = f;code = 102
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = d;code = 100
char = i;code = 105
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = n;code = 110
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char = ;code = 32
char = r;code = 114
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char = f;code = 102
char = l;code = 108
char = e;code = 101
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char = v;code = 118
char = e;code = 101
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char = e;code = 101
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char = a;code = 97
char = l;code = 108
char = s;code = 115
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char = o;code = 111
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char = f;code = 102
char = e;code = 101
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char = s;code = 115
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char = ;code = 32
char = w;code = 119
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char = e;code = 101
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char = i;code = 105
char = v;code = 118
char = e;code = 101
char = s;code = 115
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char = a;code = 97
char = s;code = 115
char = ;code = 32
char = b;code = 98
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char = o;code = 111
char = a;code = 97
char = n;code = 110
char = a;code = 97
char = l;code = 108
char = y;code = 121
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char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
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char = l;code = 108
char = a;code = 97
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char = f;code = 102
char = o;code = 111
char = r;code = 114
char = m;code = 109
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = T;code = 84
char = h;code = 104
char = e;code = 101
char = ;code = 32
char = p;code = 112
char = o;code = 111
char = t;code = 116
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = o;code = 111
char = f;code = 102
char = ;code = 32
char = g;code = 103
char = o;code = 111
char = l;code = 108
char = d;code = 100
char = ,;code = 44
char = ;code = 32
char = c;code = 99
char = a;code = 97
char = r;code = 114
char = b;code = 98
char = o;code = 111
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char = ,;code = 44
char = ;code = 32
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char = n;code = 110
char = d;code = 100
char = ;code = 32
char = a;code = 97
char = l;code = 108
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char = m;code = 109
char = i;code = 105
char = n;code = 110
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char = m;code = 109
char = ;code = 32
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char = o;code = 111
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char = -;code = 45
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char = f;code = 102
char = l;code = 108
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = i;code = 105
char = t;code = 116
char = y;code = 121
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = m;code = 109
char = p;code = 112
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char = c;code = 99
char = t;code = 116
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char = d;code = 100
char = i;code = 105
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char = c;code = 99
char = s;code = 115
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char = a;code = 97
char = s;code = 115
char = ;code = 32
char = a;code = 97
char = ;code = 32
char = s;code = 115
char = u;code = 117
char = b;code = 98
char = s;code = 115
char = t;code = 116
char = r;code = 114
char = a;code = 97
char = t;code = 116
char = e;code = 101
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char = f;code = 102
char = o;code = 111
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char = ;code = 32
char = m;code = 109
char = i;code = 105
char = c;code = 99
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char = a;code = 97
char = s;code = 115
char = s;code = 115
char = a;code = 97
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char = n;code = 110
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char = i;code = 105
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char = s;code = 115
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char = t;code = 116
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char = .;code = 46
char = ;code = 32
char = C;code = 67
char = D;code = 68
char = ;code = 32
char = p;code = 112
char = o;code = 111
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char = c;code = 99
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char = b;code = 98
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char = a;code = 97
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char = e;code = 101
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char = s;code = 115
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = ;code = 32
char = c;code = 99
char = o;code = 111
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = t;code = 116
char = h;code = 104
char = e;code = 101
char = s;code = 115
char = e;code = 101
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char = r;code = 114
char = e;code = 101
char = f;code = 102
char = l;code = 108
char = e;code = 101
char = c;code = 99
char = t;code = 116
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char = n;code = 110
char = g;code = 103
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = t;code = 116
char = e;code = 101
char = r;code = 114
char = i;code = 105
char = a;code = 97
char = l;code = 108
char = s;code = 115
char = ,;code = 44
char = ;code = 32
char = m;code = 109
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = t;code = 116
char = a;code = 97
char = i;code = 105
char = n;code = 110
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = ;code = 32
char = o;code = 111
char = p;code = 112
char = t;code = 116
char = i;code = 105
char = c;code = 99
char = a;code = 97
char = l;code = 108
char = ;code = 32
char = p;code = 112
char = r;code = 114
char = o;code = 111
char = p;code = 112
char = e;code = 101
char = r;code = 114
char = t;code = 116
char = i;code = 105
char = e;code = 101
char = s;code = 115
char = .;code = 46
char = ;code = 32
char = P;code = 80
char = r;code = 114
char = o;code = 111
char = b;code = 98
char = e;code = 101
char = s;code = 115
char = ;code = 32
char = i;code = 105
char = m;code = 109
char = m;code = 109
char = o;code = 111
char = b;code = 98
char = i;code = 105
char = l;code = 108
char = i;code = 105
char = z;code = 122
char = a;code = 97
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = o;code = 111
char = n;code = 110
char = t;code = 116
char = o;code = 111
char = ;code = 32
char = r;code = 114
char = e;code = 101
char = f;code = 102
char = l;code = 108
char = e;code = 101
char = c;code = 99
char = t;code = 116
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = l;code = 108
char = a;code = 97
char = y;code = 121
char = e;code = 101
char = r;code = 114
char = s;code = 115
char = ;code = 32
char = w;code = 119
char = a;code = 97
char = s;code = 115
char = ;code = 32
char = s;code = 115
char = t;code = 116
char = u;code = 117
char = d;code = 100
char = i;code = 105
char = e;code = 101
char = d;code = 100
char = ;code = 32
char = b;code = 98
char = y;code = 121
char = ;code = 32
char = b;code = 98
char = o;code = 111
char = t;code = 116
char = h;code = 104
char = ;code = 32
char = p;code = 112
char = a;code = 97
char = s;code = 115
char = s;code = 115
char = i;code = 105
char = v;code = 118
char = e;code = 101
char = ;code = 32
char = a;code = 97
char = d;code = 100
char = s;code = 115
char = o;code = 111
char = r;code = 114
char = p;code = 112
char = t;code = 116
char = i;code = 105
char = o;code = 111
char = n;code = 110
char = ;code = 32
char = a;code = 97
char = n;code = 110
char = d;code = 100
char = ;code = 32
char = c;code = 99
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char = a;code = 97
char = l;code = 108
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = l;code = 108
char = i;code = 105
char = n;code = 110
char = k;code = 107
char = i;code = 105
char = n;code = 110
char = g;code = 103
char = .;code = 46
char = ;code = 32
char = D;code = 68
char = i;code = 105
char = f;code = 102
char = f;code = 102
char = e;code = 101
char = r;code = 114
char = e;code = 101
char = n;code = 110
char = t;code = 116
char = ;code = 32
char = c;code = 99
char = h;code = 104
char = e;code = 101
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char = i;code = 105
char = c;code = 99
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char = ;code = 32
char = m;code = 109
char = o;code = 111
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char = f;code = 102
char = i;code = 105
char = c;code = 99
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char = t;code = 116
char = i;code = 105
char = o;code = 111
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char = s;code = 115
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char = w;code = 119
char = e;code = 101
char = r;code = 114
char = e;code = 101
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char = a;code = 97
char = d;code = 100
char = e;code = 101
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char = b;code = 98
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char = e;code = 101
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char = t;code = 116
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char = p;code = 112
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char = e;code = 101
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char = f;code = 102
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char = T;code = 84
char = h;code = 104
char = u;code = 117
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char = p;code = 112
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char = f;code = 102
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char = 1;code = 49
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char = a;code = 97
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char = g;code = 103
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char = r;code = 114
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char = a;code = 97
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char = s;code = 115
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char = i;code = 105
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char = n;code = 110
char = ;code = 32
char = w;code = 119
char = i;code = 105
char = t;code = 116
char = h;code = 104
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char = N;code = 78
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char = ;code = 32
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char = p;code = 112
char = o;code = 111
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char = ;code = 32
char = w;code = 119
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char = ;code = 32
char = s;code = 115
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char = e;code = 101
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char = s;code = 115
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char = n;code = 110
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char = t;code = 116
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result sql = Compact disc (CD) surface modification with different reflective materials offers new working perspectives as bioanalytical platforms. The potential of gold, carbon, and aluminum low-reflectivity compact discs as a substrate for microassaying is presented. CD polycarbonate bases were coated with these reflecting materials, maintaining optical properties. Probes immobilization onto reflective layers was studied by both passive adsorption and covalent linking. Different chemical modifications were made on substrates to provide functional groups capable of anchoring probes with primary amines covalently. Thus, self-assembled monolayers were performed by chemisorption of 11-mercaptoundecanoic acid on gold surfaces, and silanization with N-(trimethoxysilylpropyl)ethylenediamine triacetic acid on aluminum to provide a carboxylic acid functional group. Carbon oxidation with oxygen plasma afforded similar functionalization on the discs coated with this material. Performance of the studied materials as reflective layers was evaluated, and as proof of concept, a microimmunoassay for a neurotoxic compound was studied on the three surfaces. The results show the possibility of doing assays on this new supports with good analytical performances while maintaining discs' optical and mechanical properties to be read by a CD player.

Publication: Anal. Chem. 2013 Apr 16 ;85(8)::4178-86. :doi: 10.1021/ac4004985.
Publication Date: 2013 Apr 16
Study Author(s): Brun, Eva M;Puchades, Rosa;Maquieira, Ángel;
Institution: Centro de Reconocimiento Molecular y Desarrollo Tecnológico, Departamento de Química, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain.
Shortcut link to this study:
Compact disc (CD) surface modification with different reflective materials offers new working perspectives as bioanalytical platforms. The potential of gold, carbon, and aluminum low-reflectivity compact discs as a substrate for microassaying is presented. CD polycarbonate bases were coated with these reflecting materials, maintaining optical properties. Probes immobilization onto reflective layers was studied by both passive adsorption and covalent linking. Different chemical modifications were made on substrates to provide functional groups capable of anchoring probes with primary amines covalently. Thus, self-assembled monolayers were performed by chemisorption of 11-mercaptoundecanoic acid on gold surfaces, and silanization with N-(trimethoxysilylpropyl)ethylenediamine triacetic acid on aluminum to provide a carboxylic acid functional group. Carbon oxidation with oxygen plasma afforded similar functionalization on the discs coated with this material. Performance of the studied materials as reflective layers was evaluated, and as proof of concept, a microimmunoassay for a neurotoxic compound was studied on the three surfaces. The results show the possibility of doing assays on this new supports with good analytical performances while maintaining discs' optical and mechanical properties to be read by a CD player.
PMID: 23489115

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